• Title/Summary/Keyword: environmental induction

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Effect of Shading Level on the Induction of Inflorescence and Growth of Phalaenopsis Hybrid (차광 수준이 팔레놉시스의 화경 발생과 생육에 미치는 영향)

  • Lee, Dong Soo;Yae, Byeong Woo;Lee, Yong Beom;Lee, Young Ran
    • FLOWER RESEARCH JOURNAL
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    • v.19 no.1
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    • pp.37-41
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    • 2011
  • This experiment was carried out to find an optimum shading level for the growth of root and shoot, and to find the effect of shading level after August on the induction and growth of inflorescence of Phalaenopsis hybrid. The shading levels were 50%, 60%, 70%, 80% and 90% of natural light($1200{\mu}molm^{-2}s^{-1})$. The $CO_2$ uptake, transpiration rate, carbohydrate content, fresh weight and dry weight of Phalaenopsis hybrid were higher at 50-60% level than the others. But, it was diminished when the shading level was increased from 70% to 90%. Inflorescence length, the number of inflorescence and flower per plant all increased under 50-60% shading level and the day needed for the flowering after treatment decreased. Especially, the induction of inflorescence was depressed and flowering is not occurred under 90% during experiment period. These results suggest that the optimal shading level for the growth of Phalaenopsis including inflorescence was founded to be 50-60% in the season of light intensity and amount of sunshine decrease after august.

A Basic Experimental Study on Vibration Power Generator for A Green Traffic Network (녹색교통망을 위한 진동력 발전 기초 실험연구)

  • Jo, Byung-Wan;Lee, Yun-Sung;Kim, Young-Ji;Park, Jung-Hoon
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.29 no.6D
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    • pp.675-683
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    • 2009
  • A Basic experimental study for the development of vibration-power generation system from the vibration energy of urban infrastructure, such as a railroad, highway, and bridges, was carried out to harvest electricity from moving vehicles. Starting with the proposal of vibration power generator which converts vibration energy to an electric power by using self-induction technology, the research explains the basic concept of self-induction technology and the dynamic characteristics of a ibration power generator. Also, in order to analyze the correlation of an electromotive force from vibration power generator which depends on external force and vibration speeds, many indoor experiments with various variables were achieved. Based on the experimental results, a vibration power generator system's ability were analyzed. With those results, basic data of vibration power generator system to acquire the maximum available power was confirmed.

A Whole Cell Bioluminescent Biosensor for the Detection of Membrane-Damaging Toxicity

  • Park, Sue-Hyung;Gu, Man-Bock
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.4 no.1
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    • pp.59-62
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    • 1999
  • The recombinant bacteria strain DPD2540, containing a fabA::luxCDABE fusion, was used to detect the toxicity of various chemicals in this study. Membrane damaging agents such as phenol, ethanol, and cerulenin induced a rapid bioluminescent response from this strain. Other toxic agents, such as DNA-damaging or oxidative-damaging chemicals, showed a delayed bioluminescent response in which the maximum peak appeared over 150 min after induction. This strain was also tested for measurement of toxicity in field samples such as wastewater and river water effluents.

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Genotoxicily Studies of An Anticancer Agent of Camptothecin Series, CKD-602 (Camptothecin계 항암제 CKD-602의 유전독성평가)

  • 하광원;오혜영;허옥순;박장환;손수정;한의식;김종원;강일현;강혁준
    • Environmental Mutagens and Carcinogens
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    • v.18 no.2
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    • pp.129-134
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    • 1998
  • To evaluate the genotoxicity of CKD-602, an anticancer agent the in viかo reverse mutation assay using Salmonella typhimurium, the Chromosome aberration assay using Chinese hamster lung (CHL) cells and the in vivo micronucleus assay using bone marrow cells of ddY mice were performed. In the reverse mutation assay, CKD-602 did not induced mutagenicity in Salmonella typhimurium TA 98, TA 100, TA 1535, and TA 1537 strains with and without metabolic activation. In the chromosome aberration test using CHL cells, there was an increased incidence of structural aberrations induced by CKD-602 without metabolic activation during 24 and 48 hours, but CKD-602 did not induce chromosome aberration with metabolic activation. The in vivo induction of micronuclei was measured in polychromatic erythrocytes of bone marrow of male ddY mice. At 24 hours after treatment with CED-602 by i.p. once, there was an increased incidence of micronucleated polychromatic erythrocytes in bone marrow of ddY male mice.

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Genotoxicify Studies of on Antihypertensive Agent, SKP-450 (고혈압 치료제 SKP-450의 유전독성평가)

  • 하광원;오혜영;박장환;허옥순;손수정;한의식;류근호;조용백
    • Environmental Mutagens and Carcinogens
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    • v.18 no.2
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    • pp.123-128
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    • 1998
  • To evaluate the genotoxicity of SKP-450, an antihypertensive agent the in vitro reverse mutation assay using Salmonella typhimurium, the Chromosome aberration assay using Chinese hamster lung (CHL) cells and the in vivo micronucleus assay using bone marrow cells of ddY mice were performed. In the Reverse mutation test, SKP-450 did not induced mutagenicity in Salmonella typhimurium TA 98, TA 100, TA 1535 and TA 1537 with and without metabolic activation. In the chromosome aberration assay using CHL cells, there was no increased incidence of structural and numerical aberrations with and without metabolic activation. The in vivo induction of micronuclei was measured in polychromatic erythrocytes of bone marrow of male ddY mice at 30 hours after treatment with SKP-450 by p.o once. The results showed no increased incidence of micronucleated polychromatic erythrocytes in bone marrow of ddY male mice treated with SKP-450.

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Effects of($\pm$)-camphor on Hepatic Cytochrome P450 Enzymes in Male ICR Mice (($\pm$)-Camphor가 ICR 마우스 수컷의 간 cytochrome P450 효소 활성에 미치는 영향)

  • 오은경;박형건;배기헌;최옥진;최은경;최창근;한진희;정태천
    • Environmental Analysis Health and Toxicology
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    • v.17 no.4
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    • pp.307-314
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    • 2002
  • Effects of ($\pm$)-camphor on liver cytochrome P450 enzymes were investigated in male ICR mice. Mice were treated intraperitoneally with 0, 200, 400 and 800 mg/kg of ($\pm$)-camphor in corn oil for 3 consecutive days. Twenty four hr after the final treatment, the animals were subjected to necropsy. The activities of serum aspartate aminotransferase and serum alanine aminotransferase were slightly changed by the treatment with ($\pm$)-camphor at the doses used. Administration of ($\pm$)-camphor to mice significantly induced the hepatic activities of pentoxyresorufin O-depentylase and benzyloxyresorufin O-debenzylase and weakly induced ethoxyresorufin O-deethylase in dose-dependent manners. The present results suggested that ($\pm$)-camphor might act as a relatively specific inducer of hepatic cytochrome P450 2B in male ICR mice.

Preventive Efficacy of Kam Doo Extract Against Dichlovos Intoxication in Mouse (유기인중독에 대한 감두추출액의 방제효과)

  • 김학엽;임국환
    • Journal of Environmental Health Sciences
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    • v.15 no.2
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    • pp.75-83
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    • 1989
  • To evaluate the effect of Kam Doo-extract (KDE) on organophosphorous (OP)intoxication in mouse, this research was conducted. KDE prescribed with the equal weights of both Padix Glycyrrizae and Simen Glycine was extracted in water at 100$^{\circ}$C for 2hr, and concentrated in a vacuum evaporator. Animmal used in this research was ICR-strained male mice (bodyweight: 20 ~ 25g), and induction material for OP intoxication was DDVP(Dichlovos). Toxicity parameters used to evaluate KDE-preventive effect on DDVP were cholinesterase activity, and protection index of KDE against LDso values of DDVP. As the results, KDE prevented the inhibition of cholinesteranse activity due to DDVP-treatment, and inhanced the protecion index. Consequently our experimental data show the KDE will be useful as an preventive agent in respect that KDE is safe and effective against OP intoxication in mouse.

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Biodegradation and Kinetics of Trichloroethylene by Micrococcus sp. MS-64K (Micrococcus sp. MS-64K에 의한 Trichloroethylene의 분해특성 및 Kinetics)

  • 김종수;박근태
    • Journal of Environmental Science International
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    • v.6 no.5
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    • pp.481-488
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    • 1997
  • Microorganisms capable of degrading trlchloroethylene(TCEI using phenol as a induction substrate were isolated from industrial effluents and soil. The strain MS-64K which had the highest blodegradablllty was identified as the genus Micrococcus. The optimal conditions of medium for the growth and blodegadatlon of trlchloroethylene were observed as follows; the initial pH 7.0, trlchloroethylene 1, 000ppm as the carbon source, 0.2% ${(NH_4)}_2SO_4$, as the nitrogen source. respectively. Lag period and degradation time on optimal medium were shorter than those on Isolation medium. Growth on the optimal medium was Increased. Addition of 0.1% Triton X-100 Increased the growth rate of Micrococcus sp. MS-64K, but degradation was equal to optimal medium. Trlchloroethylene degradation by Micrococcus sp. MS-64K was shown to fit logarithmic model when the compound was added at initial concentration of 1, 000ppm.

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Induction of Oxidative Stress by Silver Nanoparticles in Cultured Leydig Cells (배양 레이디히 세포를 이용한 은나노 물질의 산화적 스트레스발생 연구)

  • Park, Eun-Jung;Park, Kwang-Sik
    • Environmental Analysis Health and Toxicology
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    • v.22 no.1 s.56
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    • pp.57-64
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    • 2007
  • Nanomaterials have been used to create unique devices at the nanoscale level. However, the toxicities of nanomaterials have not been fully tested and the risk of nanomaterials has been raised as an emerging issue in these days. In this study, the cytotoxicity of silver nanoparticles was tested using cultured mouse leydig cells. As results, silver nanoparticles showed cytotoxicity with the generation of reactive oxygen species (ROS). With the increased level of ROS, intracellular glutathione level was decreased. DNA fragmentation and caspase-3 activation suggested the apoptotic mechanism of cell death in leydig cells treated with silver nanoparticles.