• 제목/요약/키워드: environmental induction

검색결과 773건 처리시간 0.025초

Inhibition of Seed Germination and Induction of Systemic Disease Resistance by Pseudomonas chlororaphis O6 Requires Phenazine Production Regulated by the Global Regulator, GacS

  • Kang, Beom-Ryong;Han, Song-Hee;Zdor, Rob E.;Anderson, Anne J.;Spencer, Matt;Yang, Kwang-Yeol;Kim, Yong-Hwan;Lee, Myung-Chul;Cho, Baik-Ho;Kim, Young-Cheol
    • Journal of Microbiology and Biotechnology
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    • 제17권4호
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    • pp.586-593
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    • 2007

  • Seed coating by a phenazine-producing bacterium, Pseudomonas chlororaphis O6, induced dose-dependent inhibition of germination in wheat and barley seeds, but did not inhibit germination of rice or cucumber seeds. In wheat seedlings grown from inoculated seeds, phenazine production levels near the seed were higher than in the roots. Deletion of the gacS gene reduced transcription from the genes required for phenazine synthesis, the regulatory phzI gene and the biosynthetic phzA gene. The inhibition of seed germination and the induction of systemic disease resistance against a bacterial soft-rot pathogen, Erwinia carotovora subsp. carotovora, were impaired in the gacS and phzA mutants of P chlororaphis O6. Culture filtrates of the gacS and phzA mutants of P. chlororaphis O6 did not inhibit seed germination of wheat, whereas that of the wild-type was inhibitory. Our results showed that the production of phenazines by P. chlororaphis O6 was correlated with reduced germination of barley and wheat seeds, and the level of systemic resistance in tobacco against E. carotovora.

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Construction of a Reporter Strain Pseudomonas putida for the Detection of Oxidative Stress Caused by Environmental Pollutants

  • Lee Yun-Ho;Ahn Eun-Young;Park Sung-Su;Madsen Eugene L.;Jeon Che-Ok;Park Woo-Jun
    • Journal of Microbiology and Biotechnology
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    • 제16권3호
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    • pp.386-390
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    • 2006

  • A green fluorescent protein-based Pseudomonas putida reporter was successfully constructed and shown to be capable of detecting oxidative stress. In this whole-cell reporter, the promoter of the paraquat-inducible ferredoxin-$NADP^+$ reductase (fpr) was fused to a promoterless gfp gene on a broad-host-range promoter probe vector. Pseudomonas putida KT2440 harboring this reporter plasmid exhibited an increased level of gfp expression in the presence of redox-cycling agents (paraquat and menadione), hydrogen peroxide, and potential environmental pollutant chemicals such as toluene, paint thinner, gasoline, and diesel. Induction of fpr in the presence of these chemicals was confirmed using Northern blot analysis.

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Identification of Proteineous Biomarkers for Cadmium- and Ceramide- Induced Toxicity in Human Brain Cells through Display Proteomic Analysis

  • Oh, Mi-Jung;Chae, Kyu-Young;Park, Mi-Ja;Cho, Dong-Hawn;Kim, Dae-Kyong
    • 대한약학회:학술대회논문집
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    • 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-2
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    • pp.104.2-104.2
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    • 2003

  • Cadmium is an environmental pollutant and exhibits nephrotoxicity, hepatotoxicity and immunotoxicity. Recently, cadmium was found to induce DNA fragmentation, a biochemical hallmark of apoptosis, in cultured renal cells, hepatocytes and neuroblastoma cell. Therefore, the various toxicities of cadmium are thought to be caused by the induction of apoptosis. Lipids-derived pro-apoptotic ceramide has emerged as an important intracellular signaling molecule that mediates diverse cellular effects, of which programmed cell death, or apoptosis, has attracted significant interest. (omitted)

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