• 제목/요약/키워드: environmental biotechnology

검색결과 4,113건 처리시간 0.033초

조건불리지역 재배를 위한 산화스트레스 내성 고구마 품종의 선발 (Selection of oxidative stress-tolerant sweetpotato cultivars for cultivation on marginal lands)

  • 김윤희;박성철;양경실;주지린;자오동란;마다이푸;정재철;이행순;곽상수
    • Journal of Plant Biotechnology
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    • 제36권3호
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    • pp.219-223
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    • 2009
  • Oxidative stress derived from excess reactive oxygen species (ROS) is a major damaging factor for plants exposed to environmental stresses. Sweetpotato [Ipomoea batatas (L.) Lam] has a relatively broad adaptability to harsh environmental conditions compared to other staple crops. In this study, to select stress-tolerant sweetpotato cultivars for sources of molecular breeding on marginal lands, we evaluated the ion leakage values in 10 different cultivars after treatment of methyl viologen (MV), an ROS-generating nonselective herbicide, to leaf discs. DPPH radical scavenging activity and the contents of total phenolics were also investigated. The ion leakage of each cultivar showed a diverse value, which is well correlated with DPPH radical scavenging activity of each cultivar. DPPH radical scavenging activity also showed a high corelation with the contents of total phenolic contents. Three cultivars of Yanshu 8, Shinhwangmi and Shinzami showed high antioxidant activity. Our results suggest that a simple and efficient DPPH radical scavenging activity would be a suitable method to select potential cultivars with enhanced tolerance to multiple environmental stress.

Characterization of full-length enriched expressed sequence tags of dehydration-treated white fibrous roots of sweetpotato

  • Kim, Sun-Hyung;Song, Wan-Keun;Kim, Yun-Hee;Kwon, Suk-Yun;Lee, Haeng-Soon;Lee, In-Chul;Kwak, Sang-Soo
    • BMB Reports
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    • 제42권5호
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    • pp.271-276
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    • 2009
  • Sweetpotato (Ipomoea batatas (L). Lam.) is relatively tolerant to unfavorable growth conditions such as drought, yet has not been exploited to provide a better understanding of the molecular basis of drought stress tolerance. We obtained 983 high-quality expressed sequence tags of 100 bp or longer (average length of 700 bp) from cDNA libraries of detached white fibrous root tissues by subjecting them to dehydration for 6 h. The 431 cDNAs were each assigned a function by alignment using the BLASTX algorithm. Among them, three genes associated with various abiotic stresses and nine genes not previously associated with drought stress were selected for expression pattern analysis through detailed reverse transcription-polymerase chain reaction. The direct and indirect relationships of the 12 genes with drought tolerance mechanisms were ascertained at different developmental stages and under various stress conditions.