• KSBB Journal
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• v.17 no.3
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• pp.213-227
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• 2002

To date, the majority of biosensor technologies use binding components such as enzymes antibodies, nucleic acids and protein ligands. In contrast, the goal underlying the use of cells and tissues of animals and plants for a sensor system is to obtain systems capable of extracting information based on the biological activity, mechanisms of action and consequences of exposure to a chemical or biological agent of interest. These systems enable the interrogation of more complex biological response and offer the potential to gather higher information content from measuring physiologic and metabolic response. In these articles, same of the recent trends and applications of microbial biosensors in environmental monitoring and for use in food and fermentations have been reviewed. This endeavor presents many technological challenges to fabricate new microbial biosensors for other scientific field.

• KSBB Journal
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• v.15 no.5
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• pp.423-427
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• 2000

Intense research on biosensors has been performed in a number of different institution over the past 15 years, but relatively few commercial products have resultingly, the blood glucose sensor is a good example of a product which penetrated the market. However recently, the development of electrochemical and optical technologies has accelerated the turnover of the research as is illustrated by a rapid increase in the number of point-of-care diagnostic systems and analytical devices. Examples of such biosensors used in the fields of medical diagnostics, bioprocess control, and environmental monitoring are described, and summarized in an introduction to their characteristics, structures, and functions, given.

• Journal of Microbiology and Biotechnology
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• v.25 no.11
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• pp.1773-1781
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• 2015

Environmental security is one of the major concerns for the safety of living organisms from a number of harmful pollutants in the atmosphere. Different initiatives, legislative actions, as well as scientific and social concerns have been discussed and adopted to control and regulate the threats of environmental pollution, but it still remains a worldwide challenge. Therefore, there is a need for developing certain sensitive, rapid, and selective techniques that can detect and screen the pollutants for effective bioremediation processes. In this perspective, isolated enzymes or biological systems producing enzymes, as whole cells or in immobilized state, can be used as a source for detection, quantification, and degradation or transformation of pollutants to non-polluting compounds to restore the ecological balance. Biosensors are ideal for the detection and measurement of environmental pollution in a reliable, specific, and sensitive way. In this review, the current status of different types of microbial biosensors and mechanisms of detection of various environmental toxicants are discussed.

• Journal of Life Science
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• v.21 no.1
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• pp.159-164
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• 2011

Microbial whole-cell biosensors can be excellent analytical tools for monitoring environmental pollutants. They are constructed by fusing reporter genes (e.g., lux, gfp or lacZ) to inducible regulatory genes which are responsive to the relevant pollutants, such as aromatic hydrocarbons and heavy metals. A large spectrum of microbial biosensors has been developed using recombinant DNA technology and applied in fields as diverse as environmental monitoring, medicine, food processing, agriculture, and defense. Furthermore, their sensitivity and target range could be improved by modification of regulatory genes. Recently, microbial biosensor cells have been immobilized on chips, optic fibers, and other platforms of high-throughput cell arrays. This paper reviews recent advances and future trends of genetically modified microbial biosensors used for monitoring of specific environmental pollutants.

• Journal of Sensor Science and Technology
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• v.32 no.6
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• pp.403-411
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• 2023

The shift in the medical paradigm from treatment to prevention and diagnosis has underscored the growing significance of biosensors. Notably, the recent COVID-19 pandemic has spurred the widespread adoption of biosensors for the detection of viral genes and antigens. Consequently, there has been a substantial increase in both the demand for biosensors and the industries associated with their production. Furthermore, biosensors find applications not only in healthcare but also in diverse fields such as environmental monitoring, food quality control, military defense, and industrial processes. In this brief review, we delve into the essential attributes of biosensors, namely sensitivity, selectivity, and stability. We provide an overview of the latest research trends aimed at improving these attributes. Additionally, we introduce recent research cases in which these attributes are being applied both in vivo and in vitro.

• Biotechnology and Bioprocess Engineering:BBE
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• v.5 no.6
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• pp.407-412
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• 2000

Environmental biosensors and related techniques for monitoring organochlorines, endocrine disrupting chemicals and cyanobacterial toxins are described. The practical requirements for an ideal environmental biosensor are analyzed. Specific case studies for environmental applications are reported for triazines, chlorinated phenols, PCBs, microcystins, and endocrine disrupting chemicals. A new promising approach is reported for microcystins and alkylphenols that utilize electrooptical detection.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2000.04a
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• pp.94-99
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• 2000

Bioluminescence is being used as a prevailing reporter of gene expression in microorganisms and mammalian cells. Bacterial bioluminescence draws special attention from environmental biotechnologists since it has many advantageous characteristics, such as no requirement of extra substractes, highly sensitive, and on-line measurability. Using bacterial bioluminescence as a reporter of toxicity has replaced the classical toxicity monitoring technology of using fish or daphnia with a cutting-edge technology. Fusion of bacterial stress promoters, which control the transcription of stress genes corresponding to heat-shock, DNA-, or oxidative-damaging stress, to the bacterial lux operon has resulted in the development of novel toxicity biosensors with a short measurement time, enhanced sensitivity, and ease and convenient usage. Therefore, these recombinant bioluminescent bacteria are expected to induce bacterial bioluminescence when the cells are exposed to stressful conditions, including toxic chemicals. We have used these recombinant bioluminescent bacteria in order to develop toxicity biosensors in a continuous, portable, or in-situ measurement from for air, water, and soil environments. All the data obtained from these toxicity biosensors for these environments were found to be repeatable and reproducible, and the minimum detection level of toxicity was found to be ppb (part per billion) levels for specific chemicals.

• 한국균학회소식:학술대회논문집
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• 2016.05a
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• pp.35-35
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• 2016

A mycotoxin is a toxic secondary metabolite produced by organisms of the fungus kingdom, commonly known as molds and has been widely contaminated in agricultural products such as grains and cereals. Many methods including high performance liquid chromatography (HPLC) and gas chromatography (GC) have already been proposed and reviewed for mycotoxins. These methods are either expensive or time-consuming due to the complication of sample preparation and pre-concentration before determination. In addition, both methods are unsuitable for the routine screening of large sample numbers. A biosensor is a fictive analytical device that combines a biological component with a physicochemical detector for the detection of an analyte. Biosensors represent a rapidly expanding field, at the present time, with an estimated 60% annual growth rate; the major impetus coming from the health-care industry but with some pressure from other areas, such as food safety and environmental monitoring. Antibodies and aptamers are bioreceptors which have been used in the development of biosensors. There are many kinds of antibodies and aptamers specific to mycotoxin, and antibody (or aptamer)-based biosensors have been successfully developed for the detection of mycotoxin. The biosensors permit the rapid, sensitive, simple, and on-site detection of a range of mycotoxins and can be an alternative method to traditional methods such as HPLC and GC. This presentation provides the development trends of biosensors to mycotoxins and their application to food and agricultural products.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.36 no.6
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• pp.556-562
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• 2023

This paper presents the development and market trends of nano biosensors. These biosensors must possess high sensitivity and selectivity to effectively detect diseases. Presently, many research groups are focusing on the field-effect transistor aspect of nano biosensors, which can identify diseases such as Down syndrome, bladder cancer, breast cancer, and numerous other cancers, utilizing graphene and transition metal dichalcogenide materials. In the case of in-vitro diagnostics, the use of nano biosensors has been rapidly growing since the onset of the COVID-19 pandemic. This paper also discusses market trends and the outlook for both national and international enterprises engaged in the nano biosensor field. Nano biosensors are expected to play a beneficial and significant role soon, contributing to the early diagnosis of diseases and subsequently improving patient outcomes.

• Journal of the Microelectronics and Packaging Society
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• v.21 no.3
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• pp.19-24
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• 2014

This article reviews the recent progress in nanowell array biosensors that use the label-free detection protocol, and are detected in their natural forms. These nanowell array biosensors are fabricated by nanofabrication technologies that should be useful for developing highly sensitive and selective also reproducible biosensors. Moreover, electrochemical method was selected as analysis method that has high sensitivity compared with other analysis. Finally, highly sensitive nanobiosensor was achieved by combining nanofabrication technologies and classical electrochemical method. Many examples are mentioned about the sensing performance of nanowell array biosensors will be evaluated in terms of sensitivity and detection limit compared with other micro-sized electrode without nanowell array.