• 한국미생물·생명공학회지
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• 제37권2호
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• pp.176-182
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• 2009

안양천 공단 주변 슬러지를 미생물 접종원으로 무기염배지에 10 g/L의 자당을 첨가하여 수소 생산 균주 MeL 6-2을 분리하였다. 분리 균주 MeL 6-2은 호기성조건과 혐기성 조건에서 모두 생장하는 통성 혐기성 균주 Rhodopseudomonas sp.였다. 유기성 폐기물 내에 다량 함유되어있는 포도당과 자당의 농도변화가 수소 생산 속도 및 수소 생성효율에 미치는 영향에 대하여 알아보았다. 포도당을 1~12 g/L의 범위로 첨가할 경우 lag phase 없이 생장하였으며, 첨가량이 증가할수록 단위시간 및 단위부피당 수소 생산성 이 증가하여, 10 g/L에서 최대값인 $4.2\;mmol-H_2{\cdot}L^{-1}{\cdot}h^{-1}$을 보이고 그 이후 다소 감소하는 경향을 보였다. 균체량에 대한 수소생산수율은 $0.76{\sim}2.46\;L-H_2{\cdot}g-DCW^{-1}$의 값을 보였으며, 첨가된 기질인 포도당에 의한 수소생산수율은 $2.6{\sim}3.1\;mol-H_2{\cdot}mol-glucose^{-1}$의 범위였다. 자당을 1~12 g/L의 범위에서 첨가할 경우 약 10시간의 지체기 후 원할한 생장을 보였다. 단위시간 및 단위 세포무게 당 비수소 생산속도는 및 수소 생산수율은 자당의 첨가량이 증가할수록 증가하여 각각 $163\;mmol-H_2{\cdot}mg-DCW^{-1}{\cdot}h^{-1}$ 및 $4.5\;mol-H_2{\cdot}mol-glucose^{-1}$의 최대값을 보였다.

• 생명과학회지
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• 제16권3호
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• pp.454-458
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• 2006

출아효모인 Sacharomyces cerevisiae S288C균주를 이용한 효모의 게놈이 완성된 후 S. cerevisiae는 다양한 연구 모델로 이용되어져 왔다. 현재까지 효모를 이용한 기능 유전체학 측면에서의 연구는 laboratory strainin인 S288C 균주 또는 그 유래의 균주들이다. 그러나 자연에서 분리된 효모 또는 산업적으로 이용되어지고 있는 S. cerevisiae의 유전학 측면에서의 연구는 낮은 포자형성률 및 형질전환률, 그리고 S288C 균주와의 게놈상의 상이성 때문에 거의 이루어지지 않고 있다. 여기서 우리 연구진은 자연에서 분리된 Saccharomyces cerevisiae KNU5377 균주를 이용하여 random spore analysis를 통해 MATa 및 $MAT{\alpha}$ 타입의 각각의 haploid cell을 분리 후 이미 보고된 KanMX module를 가지고 round PCR기법에 의한 short flanking homology 기법을 이용하여 전사조절인자인 HSF1 유전자가 치환된 변이주를 구축할 수 있었다. 덧붙여, 모든 유전자에 이 기법을 적용할 수는 없다는 것을 확인하였다. 앞으로 이 변이주를 통해 기능 유전체학적인 측면에서 이 유전자의 스트레스와의 관련성을 연구하고자 한다.

• Toxicological Research
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• 제23권1호
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• pp.55-63
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• 2007

Diethylnitrosamine (DEN) is a nitrosamine compound that can induce a variety of liver lesions including hepatic carcinoma, forming DNA-carcinogen adducts. In the present study, microarray analyses were performed with Affymetrix Murine Genome 430A Array in order to identify the gene-expression profiles for DEN and to provide valuable information for the evaluation of potential hepatotoxicity. C57BL/6NCrj mice were orally administered once with DEN at doses of 0, 3, 7 and 20 mg/kg. Liver from each animal was removed 2, 4, 8 and 24 hrs after the administration. The histopathological analysis and serum biochemical analysis showed no significant difference in DEN-treated groups compared to control group. Conversely, the principal component analysis (PCA) profiles demonstrated that a specific normal gene expression profile in control groups differed clearly from the expression profiles of DEN-treated groups. Within groups, a little variance was found between individuals. Student's t-test on the results obtained from triplicate hybridizations was performed to identify those genes with statistically significant changes in the expression. Statistical analysis revealed that 11 genes were significantly downregulated and 28 genes were upregulated in all three animals after 2 h treatment at 20 mg/kg. The upregulated group included genes encoding Gdf15, JunD1, and Mdm2, while the genes including Sox6, Shmt2, and SIc6a6 were largely down regulated. Hierarchical clustering of gene expression also allowed the identification of functionally related clusters that encode proteins related to metabolism, and MAPK signaling pathway. Taken together, this study suggests that match with a toxicant signature can assign a putative mechanism of action to the test compound if is established a database containing response patterns to various toxic compounds.

• Toxicological Research
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• 제24권1호
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• pp.29-36
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• 2008

The present study investigated the anti-proliferative and chemosensitizing effects of Crinum asiaticum var. japonicum against multi-drug resistant (MDR) cancer cells. The 80% methanol extract, chloroform ($CHCl_3$) fraction and butanol (BuOH) fraction of C. asiaticum inhibited the growth of mitoxantrone (MX) resistant HL-60 (HL-60/MX2) cells. When HL-60/MX2 cells were treated with the $CHCl_3$ and BuOH fractions, DNA ladder and sub-G1 hypodiploid cells were observed. Furthermore, the fractions reduced BcI-2 mRNA levels, whereas Bax mRNA levels were increased. These results suggest that the inhibitory effect of C. asiaticum on the growth of the HL-60/MX2 cells might arise from the induction of apoptosis. Treatment of HL-60/MX2 cells with the fractions markedly decreased the mRNA levels of the multi-drug resistance protein-1 and breast cancer resistance protein. The $CHCl_3$ fraction and hexane fraction increased MX accumulation in HL-60/MX2 cells. These results imply that the $CHCl_3$ fraction of C. asiaticum plays a pivotal role as a chemosensitizer. We suggest that components of C. asiaticum might have a therapeutic potential for the treatment of MDR leukemia.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2003년도 식물바이오벤처 페스티발
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• pp.65-71
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• 2003

Bax, a mammalian pro-apoptotic member of the Bcl-2 family, induces cell death when expressed in yeast. To investigate whether Bax expression can induce cell death in plant, we produced transgenic Arabidopsis plants that contained murine Bax cDNA under control of a glucocorticoid-inducible promoter. Transgenic plants treated with dexamethasone, a strong synthetic glucocorticoid, induced Bax accumulation and cell death, suggesting that some elements of cell death mechanism by Bax may be conserved among various organisms. Therefore, we developed novel yeast genetic system, and cloned several Plant Bax Inhibitors (PBIs). Here, we report the function of two PBIs in detail. PBI1 is ascorbate peroxidase (sAPX). Fluorescence method of dihydrorho-damine 123 oxidation revealed that expression of Bax in yeast cells generated reactive oxygen species (ROS), and which was greatly reduced by co-expression with sAPX. These results suggest that sAPX inhibits the generation of ROS by Bax, which in turn suppresses Baxinduced cell death in yeast. PBI2 encodes nucleoside diphosphate kinase (NDPK). ROS stress strongly induces the expression of the NDPK2 gene in Arabidopsis thaliana (AtNDPK2). Transgenic plants overexpressing AtNDPK2 have lower levels of ROS than wildtype plants. Mutants lacking AtNDPK2 had higher levels of ROS than wildtype. $H_2O_2$ treatment induced the phosphorylation of two endogenous proteins whose molecular weights suggested they are AtMPK3 and AtMPK6. In the absence of $H_2O_2$ treatment, phosphorylation of these proteins was slightly elevated in plants overexpressing AtNDPK2 but markedly decreased in the AtNDPK2 deletion mutant. Yeast two-hybrid and in vitro protein pull-down assays revealed that AtNDPK2 specifically interacts with AtMPK3 and AtMPK6. Furthermore, AtNDPK2 also enhances the MBP phosphorylation activity of AtMPK3 in vitro. Finally, constitutive overexpression of AtNDPK2 in Arabidopsis plants conferred an enhanced tolerance to multiple environmental stresses that elicit ROS accumulation in situ. Thus, AtNDPK2 appears to play a novel regulatory role in $H_2O_2$-mediated MAPK signaling in plants.

• 한국토양비료학회지
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• 제41권6호
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• pp.415-418
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• 2008

폐기물 연용토양의 미생물 군집 분석을 위해 공단지역 하수 슬러지 처리등 16처리(3수준)에 대하여 가장 좋은 방법을 찾고자, RFLP, CLSU, PLFA, TGGE 의 4가지 방법을 이용하였으며, 이들 4가지 방법에 대하여 시기별 군집변동 조사를 통한 통계적 비교 분석을 하였다. 그 결과, 미생물의 경시적 군집 변동상 관찰은 PLFAs (Phospholipid Fatty Acids) 방법이 가장 효율적이었으며 RFLP는 많은 종류의 제한 효소의 사용이 필요하다. TGGE는 DNA 추출을 통해 비배양학적인 분석에서는 가장 좋은 방법이나 PCR조건 등 실험 과정에서 토양종류에 따라 민감하게 반응하여 비슷한 조성의 토양분석에 효율적이라고 생각한다.

• 한국식품영양과학회지
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• 제38권8호
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• pp.1003-1007
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• 2009

부추를 암 예방 식품 소재로 활용하기 위하여 부추로부터 주요 생리활성 물질인 함황화합물을 분리하여 인체 암세포성장 억제 및 간암세포의 사멸이 apoptosis에 의해 유도되는지를 조사하였다. 부추 함황화합물을 1, 5, 10, 20 및 30 $\mu g$/mL 농도로 24, 48 및 72시간별로 간암(HepG2) 및 폐암세포 (A549)에 처리하여 암세포 증식억제 효과를 측정한 결과 HepG2 및 A549 세포에서 농도 및 시간 의존적으로 그 성장을 억제하였으며, 20 $\mu g$/mL 농도 이상에서 암세포 성장이 60% 이상 억제되었다. 또한 부추 함황화합물 30 $\mu g$/mL 농도로 처리 시 대조군에 비하여 폐암 및 간암 세포수 감소 및 심한 형태학적 변화가 관찰되었다. 이들 암세포의 $IC_{50}$ 값을 측정한 결과 부추 함황화합물은 폐암세포(A549)보다 간암세포(HepG2)에 더 효과가 있었다. 한편 부추 함황화합물은 30 $\mu g$/mL 농도에서 핵 응축 및 apoptotic body를 나타내었으며, 농도 의존적으로 subG1 DNA 함량이 증가함으로써 HepG2 암세포 사멸이 apoptosis에 의해 유도되는 것을 확인할 수 있었다.

• Journal of Microbiology and Biotechnology
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• 제27권3호
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• pp.561-572
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• 2017

The global commercial cultivation of transgenic crops, including glyphosate-tolerant soybean, has increased widely in recent decades with potential impact on the environment. The bulk of previous studies showed different results on the effects of the release of transgenic plants on the soil microbial community, especially rhizosphere bacteria. In this study, comparative analyses of the bacterial communities in the rhizosphere soils and surrounding soils were performed between the glyphosate-tolerant soybean line NZL06-698 (or simply N698), containing a glyphosate-insensitive EPSPS gene, and its control cultivar Mengdou12 (or simply MD12), by a 16S ribosomal RNA gene (16S rDNA) amplicon sequencing-based Illumina MiSeq platform. No statistically significant difference was found in the overall alpha diversity of the rhizosphere bacterial communities, although the species richness and evenness of the bacteria increased in the rhizosphere of N698 compared with that of MD12. Some influence on phylogenetic diversity of the rhizosphere bacterial communities was found between N698 and MD12 by beta diversity analysis based on weighted UniFrac distance. Furthermore, the relative abundances of part rhizosphere bacterial phyla and genera, which included some nitrogen-fixing bacteria, were significantly different between N698 and MD12. Our present results indicate some impact of the glyphosate-tolerant soybean line N698 on the phylogenetic diversity of rhizosphere bacterial communities together with a significant difference in the relative abundances of part rhizosphere bacteria at different classification levels as compared with its control cultivar MD12, when a comparative analysis of surrounding soils between N698 and MD12 was used as a systematic contrast study.

• 미생물학회지
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• 제41권2호
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• pp.152-156
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• 2005

신생아의 분변에서 분리한 Pediococcus acidilacti GMB7330은 내산성(pH 2-3) 및 내담즙성$(1.0\%)$을 가지며 Helicobacter pylori에 대해 항균 활성을 나타내었다. P. acidilactici GMB7330의 H. pylori에 대한 저해 특성을 H. Pylori의 균수와 urease 활성 변화, 그리고 전자현미경을 이용한 세포구조 파괴를 관찰하므로써 조사하였다. P. acidilactici GMB 7330의 배양여액(pH 4.5)은 배양 24시간만에 H. pylori의 중식을 약 4 log 감소시켰다. 이때 P. acidilactici GMB7330의 배양여액의 pH를 중성으로 조절하였을 경우에는 pH 4.5의 경우보다 항균력이 다소 감소하기는 하였으나 완전히 소실되지는 않았다. 뿐만 아니라, P. acidilactici GMB7330과 유사한 농도의 젖산을 생성하는 유산균주 Lactobacillus sp. GM7311의 경우 H. pylori에 대해서 항균력을 나타내지 않는 것으로 미루어 P. acidilactici GMB7330의 항균 활성에는 배양 중 생성된 젖산 이외의 물질이 관여하는 것으로 관찰되었다. 또한 P. acidilactici GMB7330은 pH값에 관계없이 H. pylori의 urease 활성을 $50\%$이상 감소시켰으며, P. acidilactici GMB7330의 배양여액을 처리한 H. pylori 균주는 세포벽 구조의 파괴로 인한 세포 내부물질의 유출이 관찰되었다.

• 한국독성학회:학술대회논문집
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• 한국독성학회 2002년도 국제심포지움
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• pp.88.2-98
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• 2002

A wide arry of naturally occurring substances particularly those present in dietary and medicinal plants, have been reported to possess substantial cancer chemopreventive properties. Certain phytochemicals retain strong antioxidative and anti-inflammatory properties which appear to contribute to their chemopreventive or chemoprotective activities. Inducible cyclooxygenase(COX-2) and nitric oxide synthase (iNOS) are important enzymes that mediate inflammatory processes. There is some evidence that expression of both COX-2 and iNOS is co-regulated by the eukaryotic transcription factor NF-$textsc{k}$B. Increased expression of COX-2 and/or iNOS has been associated with pathophysiology of certain types of human cancers as well as inflammatory diseases. Since inflammation is closely linked to tumor promotion, substances with potent anti-inflammatory activies are anticipated to exert chemopreventive effects on carcinogenesis, particularly in the promotion stage. An example is curcumin, a yellow pigment of turmeric (Curcuma longa L., Zingiberaceae), that strongly occurring diaryl heptanoids structurally related to curcumin have substantial anti-tumor promotional activities in two-stage mouse skin carcinogenesis. Thus, yakuchinone A [1-(4'-hydroxy-3'-methoxyphenyl)-7-phenyl-3heptanone] and yakuchinone B [1-(4'-hydroxy-3'methoxyphenyl)-7-phenylhept-1-en-3-one] present in Alpinia oxyphylla Miquel (Zingiberacease) attenuate phorbol ester-induced inflammation and papilloma formation in female ICR mice. These diarylheptanoids also suppressed phorbol ester-induced activation of epdermal ornithine decarboxylase and its mRNA expression when applied onto shaven backs of mice. Yakuchinone A and B as well as curcumin inhibited phorbol ester-induced expression of COX-2 and iNOS and their mRNA in mouse skin via inactivation of NF-$textsc{k}$B. Capsaicin, a major pungent ingredient of red pepper also attenuated phorbol ester-induced NF-$textsc{k}$B activation. Similar suppression of COX-2 and iNOS and down-regulation of NF-$textsc{k}$B activation for its DNA binding were observed with the ginsenosied Rg3 and the ethanol extract of Artemisia asiatica. We have also found that certain anti-inflammatory phytochemicals exert inhibitory effects on phorbol ester-induced COX-2 expression and NF-$textsc{k}$B activation in immortalized human breast epithelial (MCF-10A) cells in culture. One of the plausible mechanisms undelying inhibition by aforementioned phytochemicals of phorbol ester-induced NF-$textsc{k}$B activation involves interference with degragation of the inhibitory unit, I$textsc{k}$Ba, which blocks subsequent nuclear translocation of the functionally active p65 subunit of NF-$textsc{k}$B. the activation of epidermal NF-$textsc{k}$B by phorbol ester and subsequent induction of COX-2 hence appear to play an important role in intracellular signaling pathwasy leading to tumor promotion and targeted inhibition of NF-$textsc{k}$B may provide a new promising cancer chemopreventive strategy.