• Title/Summary/Keyword: environment-Friendly agriculture

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A Study on Importance-Performance of Wellbeing Fusion Menu using IPA (IPA를 활용한 웰빙 퓨전 메뉴의 중요도-성취도 연구)

  • Kang, Hye-Jung;Lee, Yeon-Jung
    • Culinary science and hospitality research
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    • v.16 no.2
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    • pp.77-95
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    • 2010
  • This study aims to analyze importance and performance factors on the quality of wellbeing fusion menu of fusion restaurants to provide helpful information for building up a detailed marketing strategy and present considerations for sales increase and more efficient business results. Importance on menu quality scored a higher level than performance on the whole in fusion restaurants. Notably, in regard to attributes of menu quality, it was found that respondents put higher stress on 'taste of food', 'sanitary of food', 'cleanliness of vessels', 'quality of menu' and 'freshness of food' than anything else. Wellbeing fusion menu which has an high intake frequency rate includes 'green vegetable noodles with black bean sauce', 'sweet pumpkin salad', 'salmon salad', and 'shrimp vegetable gratin' in that order. On the other hand, the intake frequencies of 'ovened green perilla gratin', 'pomegranate dressing tofu', 'bacon roll with glutinous rice powder', and 'pomegranate dressing bacon' were rated very low. In terms of the IPA analysis on wellbeing fusion menu quality, it was important to continuously maintain 'taste of food', 'sanitary of food', 'cleanliness of vessels', 'freshness of food', 'quality of menu', 'diet menu(low fat, low calories)', 'vegetable menu', 'nutrition of food', 'variety of menu' etc. Such items as 'price', 'distinction with existing food', and 'environment-friendly organic agriculture food material' are in need of intensive care and operation.

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Impacts of Chemical Properties on Microbial Population from Upland Soils in Gyeongnam Province (경남지역 밭 토양 화학성분이 미생물 생태에 미치는 영향)

  • Lee, Young-Han;Ha, Sang-Keun
    • Korean Journal of Soil Science and Fertilizer
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    • v.44 no.2
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    • pp.242-247
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    • 2011
  • Soil management for environment-friendly agriculture depends on the effects of soil microbial activities and soil fertility. To improve soil health for the upland crops, this study evaluated a relationship between soil chemical properties and soil microbial diversities at 25 sites in upland soils in Gyeongnam Province. The average nutrients in the upland soils were 1.7 times for available phosphorous, 1.4 times for exchangeable potassium and 1.5 times for exchangeable calcium higher compared to recommend concentrations in the upland soils. We found a significant positive correlation between the soil organic matter and the soil microbial biomass C (p<0.01). Contents of organic matter and dehydrogenase in the inclined piedmont soils were significantly higher than those in the other topographical soils (p<0.05). In addition, concentrations of organic matter and microbial biomass C in the loam soils were significantly higher than in the silt loam soils (p<0.05). In principal component analyses of chemical properties and microbial populations in the upland soils, our findings suggested that available phosphorous should be considered as potential factor responsible for the clear upland soils differentiation. The soil organic matter was positive correlation with Bacillus sp. and fungi, whereas soil pH was also positive correlation with Pseudomonas sp. in upland soils.

Phylogenetic Analysis of Native Vigna sinensis in Korea Using DNA Sequence of Internal Transcribed spacer (ITS) Region (토종 갓끈동부의 ITS1, 5.8S 및 ITS2의 염기서열을 이용한 계통 분석)

  • Seo, Pil-Soo;Lee, Sook-Young;Shin, Yong Kook
    • Journal of Life Science
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    • v.27 no.3
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    • pp.351-354
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    • 2017
  • Cowpea (Vigna unguiculata (L.) Walp.) is recognized as a potential source of protein and other nutrients. The genus Vigna includes 100 wild species of plants. Especially, Vigna unguiculata includes annual cowpeas (ssp. unguiculata) and ten wild perennial subspecies. DNA sequence of internal transcribed spacer (ITS) region was determined for Vigna sinensis, one of native plant, which was found in recent but thought to have gone extinct in Korea. The seeds of Vigna sinensis used in this study were donated from Dong-Young Jo. The DNA sequence of ITS-5.8S-ITS2 for Vigna sinensis obtained from this study was deposited as Vigna sinensis AY195581 on GenBank of NCBI (National Center for Biotechnology Information). We investigated the sequence-based phylogenetic relationships of plants related and clarified its taxonomical position. DNA similarities among subspecies including Vigna unguiculata showed the range 98 to 100% in sequence-based phylogenetic analysis using total 507 base pairs of ITS1, 5.8S and ITS2. Vigna unguiculata and subspecies were grouped independently as one cluster from other Vigna species used in the phylogenetic analysis. In this study, based on the phylogenetic analysis using the ITS1-5.8S-ITS2 sequence of Vigna sinensis, it may be concluded to be classified to one of Vigna unguiculata substrains.

Isolation and Taxonomical Characterization of Streptomyces sp. JR-24 with Antibacterial Activity of Bacterial Leaf Spot of Pepper (Xanthomonas axonopodis pv. vesicatoria) (고추 세균성 점무늬병원균(Xanthomonas axonopodis pv. vesicatoria)의 항균활성 Streptomyces sp. JR-24 균주의 분리 및 분류학적 특성)

  • Han, Song-Ih;Lee, Hyo-Jin;Whang, Kyung-Sook
    • Korean Journal of Microbiology
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    • v.46 no.4
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    • pp.359-365
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    • 2010
  • Fifty Actinobacteria strains were isolated from rhizosphere soil of Sasa borealis. In the course of screening for antibacterial activity against bacterial leaf spot of pepper (Xanthomonas axonopodis pv. vesicatoria) of isolates, 12 isolates showed strong antibiotic activity. Basis on the 16S rRNA gene sequence, they were belonging to Streptomyces cluster II. Strain JR-24 exhibited strong antibiotic activity against X. axonopodis pv. vesicatoria, had a minimum inhibitory concentration of 10 ${\mu}l$/disc. The strain JR-24 was most closely related to Streptomyces galbus $DSM40089^T$ (98.1%), Streptomyces longwoodensis $LMG20096^T$ (98%) and Streptomyces capoamus $JCM4734^T$ (97.8%). When assayed with the API 20NE and 50 CHE kit, it is positive for utilization of L-arabinose, D-fructose, D-glucose, D-galactose and hydrolysis of gelatin, protein, starch. The strains contained iso-$C_{14:0}$ (25.93%), iso-$C_{15:0}$ (10.13%), anteiso-$C_{15:0}$ (19.29%) and iso-$C_{16:0}$ (20.35%) as major fatty acids and MK-9 (H4), MK-9 (H6), and MK-9 (H8) as the isoprenoid quinone. Strain JR-24 was suggested new species of genus Streptomyces by nearest neighbors of genotypic relationships and phenotypic characterization. This study was important to microbial resources investigation for environment-friendly agriculture.

Comparison of nutrient balance and nutrient loading index for cultivated land nutrient management (농경지 양분관리를 위한 양분수지 지표와 양분부하 지표간의 비교)

  • Lee, Jun-Hyung;Yoon, Young-Man
    • Korean Journal of Environmental Biology
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    • v.37 no.4
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    • pp.554-567
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    • 2019
  • Recently, concerns regarding the environmental impact due to nutrient input in croplands have increased. Therefore, the government is promoting the introduction of a nutrient management system in croplands to solve the problem of excessive nutrient input. This study was carried out to establish nutrient indicators in regional croplands to facilitate the introduction of the national nutrient management system in Korea. The nutrient load and balance indicators for nitrogen and phosphorus were analyzed for nine provinces (Gang-won, Gyeong-gi, Chung-buk, Chung-nam, Jeon-buk, Jeon-nam, Gyeong-buk, Gyeong-nam, and Jeju). In the correlation analysis between the nutrient load and nutrient balance, the correlation coefficient (r) for nitrogen was 0.2504, which was not statistically significant at the 5% significance level. However, the correlation coefficient for phosphorus was 0.7375, which was statistically significant at the 5% significance level. In the nutrient management index, phosphorus showed mutual compatibility between the nutrient load and the nutrient balance indicators, but nitrogen showed no mutual compatibility between the nutrient load and the nutrient balance indicators. Therefore, utilization of the nutrient balance indicator, reflecting the characteristics of the agricultural environment, was more reasonable as a nutrient management index for regional nutrient management.

Evidence for Polyphyletic Origin of the Members of the Subsection IV Cyanobacteria as Determined by 16S rRNA Analysis (16S rRNA 분석에 의한 Subsection IV cyanobacteria 균주들의 다계통성 기원의 증거)

  • Shin, Yong Kook;Seo, Pil-Soo
    • Journal of Life Science
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    • v.26 no.10
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    • pp.1202-1206
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    • 2016
  • Unicellular cyanobacterial strains of Subsections I and II and filamentous cyanobacterial strains of Subsection III have been shown to be polyphyletic, heterocystous strains of Subsections IV and V, both of which were previously reported to be monophyletic. In this study, the small subunit ribosomal RNA (16S rRNA) sequences of 13 strains of cyanobacteria - one strain, Oscillatoria nigro-viridis PCC7112, of the Subsection III, 6 strains including genus Anabaena, Nostoc, Tolypothrix, Calothrix and Scytonema of the Subsection IV, and 6 strains including genus Hapalosiphon, Fischerella and Chlorogloeopsis of the Subsection V - were determined. The phylogenetic analysis of cyanobacteria was carried out using the 16S rRNA sequences. The results of the phylogenetic analyses of 16S rRNA sequences, based on Neighbour-joining, maximum-parsimony, and maximum-likelihood methods, indicated that the members of Subsection IV were not monophyletic but polyphyletic. In addition, the phylogenetic results strongly indicated that the genus Scytonema in Subsection IV could be a common ancestor of heterocystous cyanobacteria in Subsection IV and V. Furthermore, the phylogenetic analyses revealed that the genus Anabaena could be phylogenetically diverse and that cyanobacterial strains in Subsection IV might be polyphyletic, whereas those in Subsection V could be monophyletic, as reported before. The results for the genus Anabaena indicate that it should be reclassified.

Plant Growth Promotion by Purple Nonsulfur Rhodopseudomonas faecalis Strains (자색비유황세균 Rhodopseudomonas faecalis의 식물생장촉진능)

  • Lee, Eun-Seon;Song, Hong-Gyu
    • Korean Journal of Microbiology
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    • v.46 no.2
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    • pp.157-161
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    • 2010
  • Photosynthetic purple nonsulfur bacterial strains were isolated from the sediments collected from rice paddy fields and sludges of wastewater treatment plant, and their plant growth promoting capabilities were examined. Most well known phytohormones, auxin such as indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) and 5'-aminolevulinic acid (ALA) were detected by HPLC in the culture broth of these isolates. Among the isolated bacteria, Rhodopseudomonas faecalis D15 showed the highest production rate of 769.8 ${\mu}g$/mg protein of IAA, 1323 ${\mu}g$/mg protein of IBA and 7.4 mM/mg protein of ALA in the modified Biebl and Pfennig's medium. R. faecalis C9 showed the highest production rate of 20.82 ${\mu}g$/mg protein of gibberellin. In consequence, the root length and dry weight of the germinated tomato seedling treated with R. faecalis isolates were longer and heavier than those of uninoculated control after 15 days of incubation in the soil. Especially, the dry weight of germinated tomato seedling increased by 119.4% in C9-treated samples after 15 days. These purple nonsulfur bacteria may be utilized as environment-friendly biofertilizer in the agriculture.

The Effect of Activated Ion Calcium for Production of Panax ginseng Seedlings in Paddy Field (논 인삼 우량묘 생산을 위한 활성이온칼슘 처리효과)

  • Kim, Dong-Won;Kim, Jong-Yeob;You, Dong-Hyun;Kim, Chang-Su;Kim, Hee-Jun;Park, Jong-Suk;Kim, Jeong-Man;Lee, Kang-Soo
    • Korean Journal of Medicinal Crop Science
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    • v.20 no.2
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    • pp.124-128
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    • 2012
  • When ginseng seedlings are cultured in paddy fields, quality degradation and yield reduction are induced by severe plant loss with chlorosis on leaves occurred physiological disorder by excessive salt and poor drainage, rusty-root occurrence, and root rot etc. Accordingly, in order to solve these problems, this study was performed to investigate the treatment method, concentrations and time of activated ion calcium as environment-friendly agricultural materials. Activated ion calcium is an enriched and purified water-soluble mineral calcium component for absorbing quickly into plant as a highly functional calcium and it is an alkaline calcium of 37% (370 $m{\ell}$/1 ${\ell}$) concentration with pH 13. Treatment method was that ginseng seeds were sown after removing water in the shade after seed immersion for 1 minute with active ion calcium of 20-fold diluted solution, and then irrigated $4{\ell}$ per 3.3 $m^2$ with 200-fold, 400-fold, and 600-fold diluted solution before emergence on late March, and supplied 1 time on leaves with 500-fold diluted solution in June and July respectively. The disease rate by treatment of activated ion calcium was that on the treatment of soil irrigated with 200-fold diluted solution compared to non-treated soil, damping-off was 33%, anthracnose was 100% reduced and the occurrence rate of rusty-root was 30% reduced. In addition, when active ion calcium of 200-fold diluted solution were soil irrigated, first and second grade ginseng were respectively 26% and 22% produced more, compared with control.

Nematicidal Efficacy of Herbal Extracts against Meloidogyne hapla (당근뿌리혹선충에 대한 식물추출물의 살선충 효과)

  • Lee, Jung-Su;Choo, Ho-Yul;Lee, Dong-Woon
    • Korean journal of applied entomology
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    • v.50 no.4
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    • pp.315-324
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    • 2011
  • The nematicidal and egg haching inhibitory effects of extracts from 30 herbal plants (total 32 samples) against Meloidogyne hapla J2 juveniles and eggs was tested using the dipping method. At 1,000 ppm, extracts of Daphne genkwa flower buds, Eugenia caryophyllata flowers, Quisqualis indica fruits, and Zingiber officinale rhizomes produced > 80% mortality in J2 juveniles. At 125 ppm, extracts of D. genkwa and Q. indica produced 91 and 99% mortality, respectively. The toxicity of 5 selected plant extracts to M. hapla differed depending on the solvent used (i.e. hexane, methanol, hot water, or cold water). Hot water extracts of Z. officinale and Q. indica produced nematicidal efficacies of 99 and 99%, compared to 36 and 98%, respectively, with cold water extraction. Q. indica extract was highly active against M. hapla regardless of extraction method. The inhibitory effects of Areca catechu, D. genkwa, Desmodium caudatum, Pharbitis nil, Q. indica, and Z. officinale extracts on egg hatching of M. hapla was evaluated. At 1,000 ppm, D. genkwa, P. nil, and Q. indica extracts significantly reduced hatching at 7, 14 and 21 days after treatment. Numbers of juveniles in soil treated with the methanol extract D. genkwa (1,000 ppm) were significantly lower than in untreated soil in trials in pots and in a ginseng (Phanax ginseng) field. These results indicate that Q. indica extracts could be used as an environmental friendly control agent of M. hapla.

Biological Control of Beet Armyworm, Spodoptera exigua(Lepidoptera: Noctuidae) with Entomopathogenic Nematodes(Steinernematid and Heterorhabditid) in Greenhouse (시설재배지에서 곤충병원성 선충, Steinernematid와 Heterorhabditid를 이용한 파밤나방(Spodoptera exigua)의 생물적 방제)

  • Kim, Hyeong-Hwan;Cho, Sung-Rae;Lee, Dong-Woon;Lee, Sang-Myeong;Choo, Ho-Yul
    • The Korean Journal of Pesticide Science
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    • v.10 no.4
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    • pp.335-343
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    • 2006
  • Five strains of Korean entomopathogenic nematodes(EPN), steinernematids and heterorhabditids(Steinernama carpocapsae GSN1, Steinernema sp. GSNUS-10, Steinernama sp. GSNUS-14, Heterorhabditis bacteriophora Hamyang, Heterorhabditis sp. GSNUH-1) were evaluated and tried in petri dish, pot, and vegetable greenhouses for environmentally friendly control of beet armyworm, Spodoptera exigua. $LC_{50}$ values of five EPN strains against beet armyworm was different depending on nematode strain and beet armyworm instar. $LC_{50}$ value of Steinernema carpocapse GSN1(GSN1) was 3.8-5.1 infective juveniles(Ijs) in 2nd to 4th instars of beet armyworm. Pathogenicity of five EPN strains against beet armyworm different in nematode strain, concentration, application times, and vegetable species in pot and greenhouse. Steinernema spp. was more effective than Heterorhabditis spp. against beet armyworm. Two or three times of applications of EPN were found to be effective regardless of nematode strain and concentration in pot and greenhouse. ENP showed different reactions on vegetable species. Efficacy of EPN was higher on Chinese cabbage than that on cabbage and kale. GSN1 was one of the most effective nematodes and 100,000 infective juveniles per $m^2$(720,000 Ijs/$7.2m^2=1{\times}10^9$Ijs/ha) resulted in higher mortality in greenhouse.