• 제목/요약/키워드: environment resources

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Bioaugmentation with GFP-Tagged Pseudomonas migulae AN-1 in Aniline-Contaminated Aquifer Microcosms: Cellular Responses, Survival and Effect on Indigenous Bacterial Community

  • Zhao, Yongsheng;Qu, Dan;Zhou, Rui;Ma, Yunge;Wang, Hao;Ren, Hejun
    • Journal of Microbiology and Biotechnology
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    • 제26권5호
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    • pp.891-899
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    • 2016

  • The recently isolated aniline-degrading bacterium Pseudomonas migulae AN-1 was tagged with green fluorescent protein (GFP) to investigate its bioaugmentation potential against aniline-contaminated groundwater through microcosm experiments. The survival and cellular response of GFP-tagged AN-1 introduced in a lab-scale aquifer corresponded directly with aniline consumption. During the process, the GFP-tagged AN-1 biomass increased from 7.52 × 105 cells/ml to 128 × 105 cells/ml and the degradation rate of aniline was 6.04 mg/l/h. GFP-tagged AN-1 was moderately hydrophobic (41.74%-47.69%) when treated with 20-100 mg/l aniline and exhibited relatively strong hydrophobicity (55.25%-65.78%) when the concentration of aniline was ≥100 mg/l. The membrane permeability of AN-1 increased followed by a rise in aniline below 100 mg/l and was invariable with aniline above 100 mg/l. Pyrosequencing analysis showed that the relative abundance of Proteobacteria (accounted for 99.22% in the non-bioaugmentation samples) changed to 89.23% after bioaugmentation with GFP-tagged AN-1. Actinobacteria increased from 0.29% to 2.01%, whereas the abundance of Firmicutes barely changed. These combined findings demonstrate the feasibility of removing aniline in aquifers by introducing the strain AN-1 and provide valuable information on the changes in the diversity of dominant populations during bioaugmentation.

  • https://doi.org/10.4014/jmb.1511.11070 인용 PDF KSCI KPUBS HTML

농촌경관 생태계서비스 가치를 고려한 관리지표 개발 (Development of Rural Ecological Landscape Management Indicator considering Ecosystem Service Value)

  • 박미정;장도담;전정배;최진아;임창수;김은자
    • 농촌계획
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    • 제23권4호
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    • pp.127-141
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    • 2017

  • The interest on rural ecological landscape has recently grown on the part of policy makers for rural development. While considerable efforts and resources have been invested to preserve and utilize rural landscape, we still lack a systematic means to quantify and evaluate ecosystem service value of the rural landscape and management status. So this study suggests rural ecological landscape management indicator considering ecosystem service value. It consists of 28 criteria and 107 subcategories, which can be applied to rural village. And We have applied ecosystem service value assessment and resident's management status on three villages, Moondang village, Yonggye village, and Sesim village.

  • https://doi.org/10.7851/Ksrp.2017.23.4.127 인용 PDF KSCI