• 한국복합재료학회:학술대회논문집
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• 한국복합재료학회 2002년도 추계학술발표대회 논문집
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• pp.75-78
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• 2002

복합재구조물에 대한 피로수명과 잔류강도를 평가하기 위한 단순화된 방법을 소개하였다. 모델의 특성화를 위해 필요한 실험을 줄이기 위하여 강도저하 파라미터수를 피로수명의 함수로 가정하였다. 임의의 순서로 배열된 하중 스펙트럼에서 응력수준에 따른 피로수명을 추출하기위해 S-N 선도를 사용하였다. 그리고 상이한 응력비(stress ratio)에 대한 영향을 고려하기위해 Goodman 형식의 방법(fatigue envelope)을 사용하였다. 잔류강도는 하중 사이클 수와 응력진폭의 함수로 가정하였으며 임의의 하중사이클 후의 잔류강도분포는 2 모수 Weibull 분포로 표현하였다.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2006년도 춘계학술발표회 논문집(I)
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• pp.118-121
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• 2006

The research reported here is concerned with the effects of the fiber combination condition and water/cement ratio on the mechanical properties of high performance fiber-reinforced cementitious composites(HPFRCC). An experimental investigation of the behavior of steel cords(SC) and SC and Polyethylene(PE) hybrid fiber reinforced cementitious material under compressive and tensile loading is presented. In this experimental research, the tensile and compressive strength and strain capacity of HPFRCC were selected using the cylindrical specimens. The results show that W/C ratio is a significant effect factor on the compressive and tensile performance of HPFRCC. The envelope curve concept applies to hybrid fiber-reinforced cementitious composites in tension just as it does to compressive stress-strain curve of fiber-reinforced cement composites. For practical purposes, the tensile envelope curve may be taken to be the same as the monotonic tensile stress-strain curve.

• 한국복합재료학회:학술대회논문집
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• 한국복합재료학회 2004년도 춘계학술발표대회 논문집
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• pp.87-90
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• 2004

In this paper, geometrically non-linear finite element analyses were performed to study the mechanical behavior of the material system of the envelope of stratospheric airships. The microstructure of the load­bearing plain weave layer was identified and modeled. The Updated Lagrangian formulation was employed to consider the geometric non-linearity as well as the induced structural non-linearity for the fiber tows. The stress-strain behavior was predicted and the effective elastic modulus was calculated by numerical experiments. It was found the non-linear stress-strain curves were largely different from those by linear analysis with much higher non-linear elastic moduli. The difference was more distinguishable when the tow waviness was smaller.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 1988년도 학술세미나 강연집
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• pp.71-123
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• 1988

The aim of this paper is to examine the end bearing capacity of a pile in cohesionless soils. The ode of failure of soil due to pile installation is assumed from experimental observation of actual soil deformation. A new solution is proposed complying with the assumed mode of failure by employing the theory of cavity expansion. The effect of curvature of failure envelope is studied in relation to tile proposed solution. The influence of a curved failure envelope becomes larger with increasing degree of curvature and the level of confining stress. This effect in some cases or reduce the end bearing capacity by tore the 80 percent compared with that given by a straight failure envelope. For practical application of tile proposed solution, the method of determining the average volume change in the plastic zone is re-evaluated. The proposed solution is confirmed by comparing the theoretical values with experimental results obtained from model pile tests in a calibration chamber. The comparison shows that the proposed solution provides a reasonable prediction of end bearing capacity for both weak and strong grained soils.

• Journal of Microbiology and Biotechnology
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• 제22권4호
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• pp.470-478
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• 2012

Recent genome comparisons of E. coli B and K-12 strains have indicated that the makeup of the cell envelopes in these two strains is quite different. Therefore, we analyzed and compared the envelope proteomes of E. coli BL21(DE3) and MG1655. A total of 165 protein spots, including 62 nonredundant proteins, were unambiguously identified by two-dimensional gel electrophoresis and mass spectrometry. Of these, 43 proteins were conserved between the two strains, whereas 4 and 16 strain-specific proteins were identified only in E. coli BL21(DE3) and MG1655, respectively. Additionally, 24 proteins showed more than 2-fold differences in intensities between the B and K-12 strains. The reference envelope proteome maps showed that E. coli envelope mainly contained channel proteins and lipoproteins. Interesting proteomic observations between the two strains were as follows: (i) B produced more OmpF porin with a larger pore size than K-12, indicating an increase in the membrane permeability; (ii) B produced higher amounts of lipoproteins, which facilitates the assembly of outer membrane ${\beta}$-barrel proteins; and (iii) motility- (FliC) and chemotaxis-related proteins (CheA and CheW) were detected only in K-12, which showed that E. coli B is restricted with regard to migration under unfavorable conditions. These differences may influence the permeability and integrity of the cell envelope, showing that E. coli B may be more susceptible than K-12 to certain stress conditions. Thus, these findings suggest that E. coli K-12 and its derivatives will be more favorable strains in certain biotechnological applications, such as cell surface display or membrane engineering studies.

• Journal of Plant Biotechnology
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• 제31권4호
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• pp.273-278
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• 2004

Differential display 방법으로 NaCl에 의하여 발현이 증가되는 cDNA들을 분리하였으며 그중 하나가 식물유래의 outer envelope membrane protein과 높은 유사성을 보였으므로 이를 ShOEP로 명명하였다. ShOEP는 1293 bp 길이에 359개의 아미노산으로 구성된 open reading frame을 포함하고 있으며, 이로부터 추정되는 분자량은 8.9 kDa이었다. ShOEP 단백질은 애기장대의 OEP와는 40.6%, 시금치와는 38%의 유사성을 나타내었다. Northern 분석결과, ShOEP 유전자는 NaCl의 농도가 증가함에 따라 발현량이 급격히 증가하는 것으로 나타났다. 염생식물인 퉁퉁마디의 OEP는 PEG에 의하여 발현이 증가하는 반면 비염생식물인 애기장대의 OEP는 큰 차이를 보이지 않았다. 효모 complementation 실험결과 ShOEP는 NaCl에 특이적이었으며 식물의 염분스트레스 내성 기작에 직접적으로 관여하고 있음을 알 수 있었다.

• 한국지반신소재학회논문집
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• 제9권3호
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• pp.19-27
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• 2010

본 연구에서는 제주도 현무암에 대한 삼축압축시험을 실시하고 Lade(1977)의 3차원 파괴규준을 적용하여 강도정수를 산정하였다. 그리고 3차원 파괴규준에 의해 산정된 강도정수와 파괴면의 특성을 살펴보고, Mohr-Coulomb의 파괴규준으로 산정한 전단강도정수와 비교검토하였다. 삼축압축시험결과를 토대로 Lade(1977)의 3차원 파괴규준을 이용하여 파괴시의 ($I_1^3/I_3-27$)과 ($P_a/I_1$) 관계로부터 매개변수인 ${\eta}_1$ 및 m을 구하였다. 3차원 파괴면을 나타내는 정팔면체 평면에서 조면암질 현무암의 파괴면이 가장 크고 스코리아가 가장 작으며, 단면형상은 조면암질 현무암이 가장 삼각형에 가깝고 스코리아가 가장 원형에 가까운 것으로 나타났다. 정팔면체 평면에서 삼축실험결과와 Lade의 파괴포락선 및 Mohr-Coulomb의 파괴포락선을 비교한 결과 높은 응력하에서는 Lade의 파괴규준이 실험치와 일치하며, 낮은 응력하에서는 Mohr-Coulomb의 파괴규준이 실험치와 일치하는 것으로 나타났다. 그리고 Lade의 3차원 파괴면은 Mohr-Coulomb의 3차원 파괴면보다 크며, 이는 Lade의 파괴규준으로 산정된 전단강도정수가 Mohr-Coulomb의 파괴규준으로 구한 것 보다 더 크게 산정됨을 의미한다.

• Composites Research
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• 제18권2호
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• pp.30-37
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• 2005

본 논문에서는 평직구조를 갖는 성층권 비행선 기낭의 하중막재에 대한 비선형 유한요소 해석 결과를 기술하였다. 평직구조를 갖는 하중막재의 미세구조를 3차원적으로 구현하였고, Updated Lagrangian 방법을 사용하여 기하학적 비선형성을 고려하였다 계산결과, 큰 변형률에서 비선형해석으로부터 얻은 응력-변형률 곡선은 선형해석의 결과와 큰 차이를 보였다. 또한 응력-변형률 곡선으로부터 얻은 비선형 탄성계수 값은 선형 탄성계수보다 큰 값을 보였는데 그 차이는 섬유의 굴곡도가 작은 경우 더욱 두드러지게 나타났다

• Molecules and Cells
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• 제26권3호
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• pp.270-277
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• 2008

This study addresses the physiological functions of the Ran-binding protein homolog NbRanBP1 in Nicotiana benthamiana. Virus-induced gene silencing (VIGS) of NbRanBP1 caused stunted growth, leaf yellowing, and abnormal leaf morphology. The NbRanBP1 gene was constitutively expressed in diverse tissues and an NbRanBP1:GFP fusion protein was primarily localized to the nuclear rim and the cytosol. BiFC analysis revealed in vivo interaction between NbRanBP1 and NbRan1 in the nuclear envelope and the cytosol. Depletion of NbRanBP1 or NbRan1 reduced nuclear accumulation of a NbBTF3:GFP marker protein. In the later stages of development, NbRanBP1 VIGS plants showed stress responses such as reduced mitochondrial membrane potential, excessive production of reactive oxygen species, and induction of defense-related genes. The molecular role of RanBP1 in plants is discussed in comparison with RanBP1 function in yeast and mammals.

• 한국직업건강간호학회지
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• 제17권1호
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• pp.34-44
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• 2008

Purpose: This study was conducted to find out the effects of self-efficacy on job stress symptoms and coping strategies depending on perceived job stress. Method: The subjects were 447 workers employed in a manufacturing company. Demographic characteristics, self-efficacy, job stress, job stress symptoms and coping strategies were assessed by a self-administered questionnaire. Each envelope to keep the secret sealed completed questionnaires. Result: Job stress and job stress symptoms in workers with a high level of self-efficacy were lower than those of a low level of self-efficacy. Active coping strategies in workers with a high level of self-efficacy group were higher than those of a low level of self-efficacy. In multiple regression analysis, job stress symptoms were significantly higher in increasing job stress, increasing self-efficacy, office workers, manager group and increasing age. Active coping strategies were significantly higher in increasing self-efficacy, increasing career, males and decreasing job stress. Whereas passive coping strategies were significantly higher in females, increasing job stress and increasing self-efficacy. Conclusion: This study suggests that self-efficacy is a significant factor on job stress, job stress symptoms and coping strategies. Therefore, developing a job stress management program to increase self-efficacy and verifying its effects are needed.