• Title/Summary/Keyword: enhanced biomass

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Performance Assessment of Three Turfgrass Species, in Three Different Soil Types, and their Responses to Water Deficit in Reinforced Cells, Growing in the Urban Environment

  • Ow, L.F;Ghosh, S.;Chin, S.W.
    • Weed & Turfgrass Science
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    • v.4 no.4
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    • pp.338-347
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    • 2015
  • Reinforcement cells are used to aid grass growth and taken together, this serves to extend greenery beyond the conventional spaces of lawns, tree pits, gardens, and parks, and is advantageous to urban cities since space for greening is often limited. Drought has variable effects on plant life and the resilience of turf to drought resistance also varies with species. Changes in photosynthetic ability were more pronounced for media rather than grass species. The media of sand without organic matter was found to be least suited for drought resistance. Normalized difference vegetation index (NDVI) and digital image analysis (DIA) data were generally in favour of Zoysia species as oppose to A. compressus. In A. compressus, selective traits such as, a more extensive root system and lower specific leaf area (SLA) were not an underlying factor that assisted this grass with enhanced drought resistance. Generally, WUE was found to be strongly related to plant characterises such as overall biomass, photosynthetic features as well as the lushness indexes, and specific leaf area. This study found a strong relationship between WUE and a suite of plant characteristics. These traits should serve as useful selection criteria for species with the ability to resist water stress.

Coproduction of Enzymes and Beta-Glucan by Aspergillus oryzae Using Solid-State Fermentation of Brown Rice

  • Ji, Su Bin;Ra, Chae Hun
    • Journal of Microbiology and Biotechnology
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    • v.31 no.7
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    • pp.1028-1034
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    • 2021
  • The effect of medium composition on enzyme and β-glucan production by Aspergillus oryzae KCCM 12698 was investigated. Brown rice, rice bran, nitrogen, and ascorbic acid are key components of the synthetic medium used in liquid-state fermentation. To determine the optimal concentrations of these components for enzyme and β-glucan production, we conducted one factor at a time experiments, which showed that the optimal concentrations were 30 g/l brown rice, 30 g/l rice bran, 10 g/l soytone, and 3 g/l ascorbic acid. Pretreatment of brown rice for 60 min prior to inoculation enhanced fungal biomass, while increasing the production of enzymes and β-glucan using solid-state fermentation. Maximum fungal biomass of 0.76 mg/g, amylase (26,551.03 U/g), protease (1,340.50 U/g), and β-glucan at 9.34% (w/w) were obtained during fermentation. Therefore, solid-state fermentation of brown rice is a process that could enhance yield and overall production of enzymes and β-glucan for use in various applications.

Enhanced Arsenic(V) Removal from Aqueous Solution by a Novel Magnetic Biochar Derived from Dairy Cattle Manure

  • Akyurek, Zuhal;Celebi, Hande;Cakal, Gaye O.;Turgut, Sevnur
    • Korean Chemical Engineering Research
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    • v.60 no.3
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    • pp.423-432
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    • 2022
  • Magnetic biochar produced from pyrolysis of dairy cattle manure was used to develop an effective sorbent for arsenic purification from aqueous solution. Biomass and magnetized biomass were pyrolyzed in a tube furnace with 10 ℃/min heating rate at 450 ℃ under nitrogen flow of 100 cm3/min for 2 h. Biochars were characterized by SEM-EDX, BET, XDR, FTIR, TGA, zeta potential analysis. The resultant biochar and magnetic biochar were opposed to 50-100-500 ppm As(V) laden aqueous solution. Adsorption experiments were performed by using ASTM 4646-03 batch method. The effects of concentration, pH, temperature and stirring rate on adsorption were evaluated. As(V) was successfully removed from aqueous solution by magnetic biochar due to its highly porous structure, high aromaticity and polarity. The results suggest dairy cattle manure pyrolysis is a promising route for managing animal manure and producing a cost effective biosorbent for efficient immobilization of arsenic in aqueous solutions.

Effects of Fertilization on Physiological Parameters in American Sycamore (Platanus occidentalis) during Ozone Stress and Recovery Phase

  • Han, Sim-Hee;Kim, Du-Hyun;Lee, Jae-Cheon;Kim, Pan-Gi
    • Journal of Ecology and Environment
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    • v.32 no.3
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    • pp.149-158
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    • 2009
  • American sycamore seedlings were grown in chambers with two different ozone concentrations ($O_3$-free air and air with additional $O_3$) for 45 days. Both the control and the $O_3$ chambers included non-fertilized and fertilized plants. After 18 days of $O_3$ fumigation, seedlings were placed in a clean chamber for 27 days. Seedlings under ozone fumigation showed a significant decrease in pigment contents and photosynthetic activity, and a significant increase in lipid peroxidation. Fertilization enhanced physiological damage such as the inhibition of photosynthetic activity and the increase of lipid peroxidation under ozone fumigation. During the recovery phase, the physiological damage level of seedlings increased with ozone fumigation. In addition, physiological damage was observed in the fertilized seedlings. Superoxide dismutase (SOD) and glutathione reductase (GR) activities of $O_3$-treated seedlings increased up to 33.8% and 16.3% in the fertilized plants. The increase of SOD activity was higher in the fertilized plants than in the non-fertilized plants. Negative effects of ozone treatment were observed in the biomass of the leaves and the total dry weight of the fertilized sycamore seedlings. The $O_3$-treated seedlings decreased in stem, root and total dry weight, and the loss of biomass was statistically significant in the fertilized plants. In conclusion, physiological disturbance under normal nutrient conditions has an effect on growth response. In contrast, in conditions of energy shortage, although stress represents a physiological inhibition, it does not seem to affect the growth response.

Saccharification of lignocellulosics by Supercritical Water (초임계수를 이용한 목질바이오매스의 당화 특성)

  • Choi, Joon-Weon;Lim, Hyun-Jin;Jo, Tae-Su;Han, Gyu-Sung;Choi, Don-Ha
    • New & Renewable Energy
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    • v.3 no.1 s.9
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    • pp.38-45
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    • 2007
  • To characterize thermo-chemical feature of sugar conversion of woody biomass, poplar wood ($Populus\;alba{\times}glandulosa$) powder was treated with supercritical water system. Supercritical water treatment (SCWT) was performed for 60 seconds at different temperatures (subcritical zone 350; supercritical zone $300,\;400,\;425^{\circ}C$) under two pressures $230{\pm}10atm$ as well as $330{\pm}10atm$, respectively, using flow type system. After separation of solid residues from SCWT products, the monomeric sugars in aqueous part converted from poplar wood powder were quantitatively determined by high performance anionic exchange chromatography [HPAEC] equipped with PAD detector and Carbo Pac PA10 column. As the temperature treated increased, the degradation of poplar wood powder was enhanced and ca 83% of woody biomass was dissolved into the water at $425^{\circ}C$. However, the pressure didn't help the degradation of biomass components. At subcritical temperature range, xylose was first formed by degradation of xylan, which is main hemicellulose component in hardwood species, while cellulose degradation started at the transition zone between sub and supercritical conditions and was remarkably accelerated at the supercritical temperature. In the supercritical water system the maximum yield of monomeric sugars amounts to ca. 7.3% based on oven dried wood weight at $425^{\circ}C$.

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Influence of $CO_2$ on Growth and Hydrocarbon Production in Botryococcus braunii

  • Ranga Rao, A.;Sarada R.;Ravishankar G.A.
    • Journal of Microbiology and Biotechnology
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    • v.17 no.3
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    • pp.414-419
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    • 2007
  • Botryococcus braunii is a green colonial fresh water microalga and it is recognized as one of the renewable resources for production of liquid hydrocarbons. CFTRI-Bb-l and CFTRI-Bb-2 have been reported for the first time and their performance with regard to growth and biochemical profile is presented here. The present study focused on effect of carbon dioxide $(CO_2)$ on biomass, hydrocarbon, carbohydrate production, fatty acid profile, and carotenoid content in various species of B. braunii (LB-572, SAG 30.81, MCRC-Bb, N-836, CFTRI-Bb-l, and CFTRI-Bb-2) at 0.5, 1.0, and 2.0% (v/v) levels using a two-tier flask. $CO_2$ at 2.0% (v/v) level enhanced growth of the organism, and a two-fold increase in biomass and carotenoid contents was observed in all the B. braunii strains studied compared with control culture (without $CO_2$ supplementation). At 1 % and 2% (v/v) $CO_2$ concentrations, palmitic acid and oleic acid levels increased by 2.5 to 3 folds in one of the strains of B. braunii (LB-572). Hydrocarbon content was found to be above 20% at 2% $CO_2$ level in the B. braunii LB-572, CFTRI-Bb-2, CFTRI-Bb-l, and N-836 strains, whereas it was less than 20% in the SAG 30.81 and MCRC-Bb strains compared with control culture. This culture methodology will provide information on $CO_2$ requirement for growth of algae and metabolite production. B. braunii spp. can be grown at the tested levels of $CO_2$ concentration without much influence on culture pH.

Organosolv Pretreatment of Slurry Composting and Biofiltration of Liquid Fertilizer-Treated Yellow Poplar for Sugar Production

  • Kim, Ho-Yong;Gwak, Ki-Seob;Jang, Soo-Kyeong;Ryu, Keun-Ok;Yeo, Hwanmyeong;Choi, In-Gyu
    • Journal of the Korean Wood Science and Technology
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    • v.43 no.5
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    • pp.578-590
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    • 2015
  • The present study examines the influence of slurry composting and biofiltration liquid fertilizer (SCBLF) treatment on the biomass characteristics of yellow poplar, and the optimization of organosolv pretreatment for sugar production. After SCBLF treatment, total exchangeable cation contents of yellow poplar was increased from $3.1g\;kg^{-1}$ to $4.4g\;kg^{-1}$, and as a result, biomass production of yellow poplar was also enhanced by 82.3%. Organosolv pretreatment was conducted with three independent variables: 1) reaction temperature: $133.2^{\circ}C$ to $166.8^{\circ}C$; 2) acid concentration: 0.2% to 1.8%; and 3) reaction time: 1.6 min to 18.4 min. Reaction temperature was the most significant variable in water insoluble solid (WIS) recovery rate. High overall sugar yield was attained from pretreatment conditions approximately 50% of WIS recovery rate, and the highest overall glucose yield (44.0%) was achieved from pretreatment at $140^{\circ}C$ with 1.5% acid concentration for 5 min. Consequently, 21.1% of glucose and 5.8% of xylose were produced from the organosolv pretreatment of SCBLF-treated 8-year-old yellow poplar.

Increased biomass and enhanced tolerance to salt stress in Chinese cabbage overexpressing Arabidopsis H+-PPase (AVP1) (애기장대 H+-PPase(AVP1) 과발현 배추에서 바이오매스 증가와 내염성 향상)

  • Park, Mehea;Won, Hee-Yeun;Kim, Chang Kil;Han, Jeung-Sul
    • Journal of Plant Biotechnology
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    • v.39 no.4
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    • pp.253-260
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    • 2012
  • On the basis of the reported agriculturally valuable phenotypes resulted from ectopic overexpression of Arabidopsis vacuolar $H^+$-PPase (AVP1), we generated the Chinese cabbage lines expressing AVP1 which then subjected to salt stress to determine the AVP1 expression if it consistently confers the capability for increasing biomass and enhancing tolerance to salinity in other species. Collectively, here we demonstrate that the transgenic young plants show more vigorous growth and higher tolerance to salt stress than wild-type ones. Increased biomass phenotype by AVP1 expression was supported by comparing fresh and dry weights of transgenic and wild type plants grown under normal condition, while higher salt tolerance trait was confirmed by tracing the kinetics of photosystem II quantum yield and DAB-staining under gradually intensified salt stress induced by MS salt or NaCl, followed by normal condition.

Species Alterations Caused by Nitrogen and Carbon Addition in Nutrient-deficient Municipal Waste Landfills

  • Kim, Kee-Dae
    • Journal of Ecology and Environment
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    • v.30 no.2
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    • pp.161-170
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    • 2007
  • The ultimate target of restoring waste landfills is revegetation. The most effective method for increasing species richness and biomass in nutrient limited waste landfills is the use of fertilizers. The aim of the present study was to investigate the effects of nitrogen fertilizer, and the addition of carbon through sawdust, sucrose and litter, on vegetation dynamics at a representative municipal waste landfill in South Korea: Kyongseodong. A total of 288 permanent plots $(0.25m^2)$ were established and treated with nitrogen fertilizer (5, 10 and $20Ng/m^2$), sawdust $(289g/m^2)$ sucrose $(222g/m^2)$ and litter $(222g/m^2)$. The aboveground biomass was significantly enhanced by nitrogen fertilizer at 5 and $10Ng/m^2$, compared with the control plots. The total cover of all plant species increased significantly on plots treated with 5 and $20Ng/m^2$, as well as on those treated with sawdust and sucrose, compared with the control plots. The higher species richness after nitrogen fertilization of 10 to $20Ng/m^2$, and the sawdust and sucrose treatment demonstrated that this was an appropriate restoration option for nutrient deficient waste landfills. This study demonstrated positive nutrient impacts on plant biomass and species richness, despite the fact that municipal waste landfills are ecosystems that are highly disturbed by anthropogenic and internal factors (landfill gas and leachate). Adequate N and C combined treatments will accelerate species succession (higher species richness and perennial increase) for restoration of waste landfills.

Auxin Affects on Production of Adventitious Roots and Secondary Metabolites in Echinacea angustifolia (오옥신 처리가 에키네시아의 부정근 및 생리활성물질 생산에 미치는 영향)

  • Jang, Young Se;Cui, Hai Yan;Lee, Eun Jung;Kim, Hae Won;Paek, Kee-Yoeup
    • Korean Journal of Medicinal Crop Science
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    • v.20 no.6
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    • pp.479-486
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    • 2012
  • The production of adventitious roots derived from root explant of Echinacea angustifolia and its secondary metabolite content were assessed in different types and levels of auxin. The induction of adventitious roots from root explant cultured in Murashige and Skoog solid medium supplemented with 1.0 mg/L indole -3-butyric acid (IBA) attained highest as 20.87 mg fresh weight and 3.07 mg dry weight per culture but root suspension culture at the same concentration of IBA enhanced biomass production as 3.07 g fresh weight and 0.38 g per culture after 4 weeks in culture. 3.0 mg/L ${\alpha}$-naphthalene acetic acid (NAA) treatment had similar effect on root biomass production as 3.07 g fresh weight and 0.38 g per culture with liquid suspension culture, whereas adventitious roots exposed to over 3.0-5.0 mg/L IBA or 5.0 mg/L NAA were less responsive by reducing the number of adventitious roots and/or changing root morphology such as short and thick. The content of secondary metabolites such as phenolic, flavonoids and total caffeic acid in adventitious roots cultured on MS medium supplemented with 1.0 mg/L IBA were attained highest as 27.20, 9.60. 10.67 mg/g dry weight, respectively. Overall, the best production of root biomass and secondary metabolites were given by 1.0 mg/L IBA.