• Korean Journal of Plant Resources
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• v.19 no.3
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• pp.427-431
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• 2006

In vitro high-frequency plant regeneration of Muscari comosum var. plumosum through somatic embryogenesis was obtained via two developmental pathways: direct embryos and multiple shoots regenerated from embryogenic callus. Flower bud with pedicel, receptacle, petal and ovary wall, floral stalk and leaf as explants were cultured in MS medium supplemented with various plant growth regulators. Embryos formed directly from pedicel, receptacle and floral stalk. Depending on explant sources, the optimal medium was MS medium supplemented with 0.2 mg/L IBA and 0.3 mg/L BA, 3.0 mg/L IBA and 3.0 mg/L BA, and MS-free medium for pedicel, receptacle, and floral stalk, respectively. Multiple shoots regenerated from embryogenic cal]i which was initiated from petal, ovary and leaf were observed in MS medium with different concentrations and combinations of hormone. The most suitable medium for each type of explant was 3.0 mg/L IBA and 3.0 mg/L BA(petal and ovary) and 5.0 mg/L IBA and 5.0 mg/L BA (leaf) Furthermore, the combination of 0.1 mg/L 2,4-D and 1.0 mg/L BA was also good for all sources of explants not only for direct embryo formation, but also, for embryogenic callus induction.

• Macromolecular Research
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• v.13 no.4
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• pp.285-292
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• 2005

We developed alginate beads loaded with transforming growth $factor-{\beta}_{1}(TGF-{\beta}_{1})$ to examine the possible application of the scaffold and cytokine carrier in tissue engineering. In this study, bone marrow stromal cells (BMSCs) and $TGF{\beta}_{1}$ were uniformly encapsulated in the alginate beads and then cultured in vitro. The cell morphology and shape of the alginate beads were observed using inverted microscope, scanning electron microscope (SEM), histological staining and RT-PCR to confirm chondrogenic differentiation. The amount of the $TGF{\beta}_{1}$ released from the $TGF-{\beta}_{1}$ loaded alginate beads was analyzed for 28 days in vitro in a phosphate buffered saline (pH 7.4) at $37^{\circ}C$. We observed the release profile of $TGF-{\beta}_{1}$ from $TGF-{\beta}_{1}$ loaded alginate beads with a sustained release pattern for 35 days. Microscopic observation showed the open cell pore structure and abundant cells with a round morphology in the alginate beads. In addition, histology and RT-PCR results revealed the evidence of chondrogenic differentiation in the beads. In conclusion, these results confirmed that $TGF-{\beta}_{1}$ loaded alginate beads provide excellent conditions for chondrogenic differentiation.

• Journal of Periodontal and Implant Science
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• v.54 no.1
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• pp.25-36
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• 2024

Purpose: Mucogingival defects (MGDs), such as dental root recessions, decreased vestibular depth, and absence of keratinized tissues, are commonly seen in dental clinics. MGDs may result in functional, aesthetic, and hygienic concerns. In these situations, autogenous soft tissue grafts are considered the gold-standard treatment. This study compares the healing process of free gingival grafts (FGGs) to bacterial cellulose matrix (BCM) and human acellular dermal matrix (ADM) seeded with fibroblasts from culture supplemented with platelet-rich plasma in a rat model. Methods: Surgical defects were made in rats, which received the following treatments in a randomized manner: group I, negative control (defect creation only); group II, positive control (FGG); group III, BCM; group IV, BCM + fibroblasts; group V, ADM; and group VI, ADM + fibroblasts. Clinical, histological, and immunological analyses were performed 15 days after grafting. Clinical examinations recorded epithelium regularity and the presence of ulcers, erythema, and/or edema. Results: The histological analysis revealed the degree of reepithelization, width, regularity, and presence of keratin. The Fisher exact statistical test was applied to the results (P<0.05). No groups showed ulcers except for group I. All groups had regular epithelium without erythema and without edema. Histologically, all groups exhibited regular epithelium with keratinization, and myofibroblasts were present in the connective tissue. The groups that received engineered grafts showed similar clinical and histological results to the FGG group. Conclusions: Within the limitations of this study, it was concluded that BCM and ADM can be used as cell scaffolds, with ADM yielding the best results. This study supports the use of this technical protocol in humans.

• Journal of the Korean Society of Food Culture
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• v.15 no.3
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• pp.163-174
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• 2000

This research was designed to develop a computer program and evaluate the nutritional balances especially the balance of fatty acids, amino acids and antioxidant vitamins for convenience foods. The Korean convenience food, Kimbab purchased from markets was evaluated by using the self-developed computer program. Contents of calories, protein and calcium were lower$(1/3^{\circ}{\neq}1/2)$ than the recommended levels of Korean adult woman, and the carbohydrate/ protein/ fat(CPF) energy ratio was 70: 13: 17. The mean P/ M/ S ratio was 2.2/ 1.4/ 1 and that of ${\omega}6/\;{\omega}3$ fatty acids was 17.9/ 1, which was higher than the desirable ratio of $4{\sim}8/\;1$. Average essential amino acid balance of market-Kimbab samples was within the desirable range even though the absolute amount of protein was lower than the recommended level. Contents of antioxidant vitamins (A, C &E) were lower than recommended levels. Two kinds of nutritionally adjusted Kimbab menu were established by self-developed computer program. Some of major changes was adding food ingredients such as tuna fish and perilla leaf cooked with sesame oil and soybean oil to increase ${\omega}3$ series fatty acids. Some fruits and milk were also added to the menu. The adjusted CPF ratios was 63: 15: 22 and the new values for P/ M/ S and ${\omega}6/\;{\omega}3$ fatty acids ratios were 1.0/ 1.2/ 1/0 and 6.1/ 1 respectively. In sensory evaluation of two kinds of adjusted Kimbab, the taste and overall estimation scores were higher than unadjusted Kimbab. The computer program developed in this study might be used as a tool for the evaluation of nutritional balance of other convenience foods and menu planning.

• Asia-Pacific Journal of Business Venturing and Entrepreneurship
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• v.15 no.1
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• pp.269-284
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• 2020

This study explored the core management competencies of ventures formed by the entrepreneur's incubator organization and startup experience in the biomedical industry in Korea. An in-depth interview was conducted with 13 entrepreneurs of biomedical ventures. Based on the previous literature, the core management competencies of the ventures, which are influenced by the incubator organization and startup experience, are classified into 'technical competency', 'organization management competency', 'network competency' and 'market pioneering competency'. Analysis of the in-depth interview has revealed 18 factors influencing the formation of the core management competencies of ventures. Qualitative factors that were not addressed by the previous empirical studies were identified in this study. These include 'confidence in technology development', 'way of performing R&D', 'organizational culture' etc. This study is characterized by its scarcity as a qualitative study that deals with the entrepreneurs' prior experience. In addition, this study categorize the core management competencies which are formed by entrepreneurs' incubator organization and startup experience as four factors. This result is expected to be useful in future research.

• Journal of Environmental Impact Assessment
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• v.24 no.5
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• pp.432-443
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• 2015

Ski industry is sensitive to climate change. Many studies were carried out to learn the impact on climate change to large scale ski resorts around the world and the results are difficult to be applied to small scale ski resorts in general. So, this study targeted small ski resorts composing the ski industry of Korea and forecasted the impact of climate change. As a result, based on the mitigation efforts to minimize climate changes of the future (RCP 4.5), ski industry could be maintained at the same level of today. However, if climate change continues at the current trend (RCP 8.5), ski resorts will face loss of business days. If 100 days are considered as the minimum days to maintain the ski business, among 17 ski resorts in Korea, 3 ski resorts will be driven out of business by 2030s, 12 more ski resorts by 2060s and remaining 2 ski resort by 2090s will end the business. It means that smaller ski resorts has higher chance of facing difficulties in running business just as large scale ski resorts. Therefore, to sustain the ski business, technical and managerial efforts to adapt to the changing environment is needed.

• Journal of the Korean Society of Marine Environment & Safety
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• v.23 no.7
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• pp.858-868
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• 2017

Korea's shipbuilding industry has led the world in the technical area over the last century. Despite this commendable performance, around 2,000 workers experience accidents almost every year with 40 being killed. This raises a question of whether the safety level of our shipbuilding industry, in particular the safety of workers, is actually at the world-class level. Accordingly, this research has analyzed several types of safety training currently provided in the field through investigating statistical data of serious accidents occurring from 2006 to 2015 in the domestic shipbuilding industry, analyzing its occurrence and causes, and conducting a survey targeting employees in the shipbuilding industry. Based on this, it has investigated problems of safety training in the shipbuilding industry and suggested improvements. First, it is essential to create a standard system for safety training in the shipbuilding industry to address problems of different kinds and levels of safety training provided by each shipyard and low quality of training, and operate more organized and systemized training. Second, safety training curriculum specializing in the shipbuilding industry should continue to be developed and standardized based on a standard system for safe training to prevent serious accidents and improve safety awareness of workers. Lastly, both employers and employees should actively provide and participate in safety training to secure safety of workers through preventing serious accidents and ultimately create safety-first culture in workplace.

• Korea Science and Art Forum
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• v.23
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• pp.95-109
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• 2016

The background and objective of this study are as follows. Gwangheemun, one of the 4 small gates of Seoul Castle is a space to represent ordinary people and it is a valuable cultural heritage that shows the process of technical transition of fortification technology during Chosun Dynasty. It is a place that we can expect to play a role as a field where history and culture mix and communicate together. But currently, the environment and facilities around Gwangheemun have fallen behind and become old, so they need to be reorganized as their local feature is not shown distinctly. We need to vitalize the new traditional space that shows local feature. This study has drawn out the method, contents and the result of study like as follows. This study aims to establish an identity based on the historical and cultural backgrounds and suggest the contents to vitalize the space of Gwangheemun as a traditional cultural heritage. By this, this study aims to create a historical and cultural space where people can enjoy, eat and look around. Therefore, based on the historical and cultural feature, it gives an identity as moonlight street, and it has developed and suggested 5 contents to vitalize space: Gwangheemun maintenance, plan, castle restoration plan, village inside the castle, village outside the castle and fashion art street. Contents to vitalize space has a meaning as a specific developmen method of urban restoration, and we can expect to be used as a direction to develop the area to enhance the cultural quality of life of both inhabitants and visitors by forming the brand identity of surrounding area with traditional cultural heritage.

• Journal of Korean Home Economics Education Association
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• v.33 no.1
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• pp.129-149
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• 2021

The purposes of this study were to examine the current status of the establishment of home economics-related departments in colleges and universities and the changes required in the home economics curriculum of secondary schools, and to structure the elective courses of home economics subject(group) that can be organized in the next high school curriculum. To achieve these purposes, related literature and data were analyzed, and a questionnaire survey and FGI were conducted by home economics experts. The research results are as follows. First, home economics was considered to be highly related not only to the human ecology but also to social sciences, education, engineering, and arts and physical education. The numbers of technical colleges and 4-year universities with departments related to home economics were 1,405 and 961 respectively in 2019. Therefore, it was confirmed that there is a sufficient basis for opening home economics subject(group) elective courses in high school. Second, in the secondary school home economics curriculum, the concepts of culture, relations, independence, and sustainability were emphasized based on the changing life patterns and values. It was proposed that the contents of the home economics course would be structured in a way that allows deep and high-level thinking and helps students to enjoy culture. This demand can be implemented by diversifying, specializing, and structuring the elective courses of the home economics subject(group). Third, a total of 18 elective subjects and subject outlines were structured in the fields of child/family, food/nutrition, clothing, housing, consumption/family management, and home economics integration. This study results will contribute to the establishment of the high school credit system by providing basic information for organizing the next home economics curriculum, and expanding the options for home economics subject(group) to high school students.

• Journal of the Korean Institute of Traditional Landscape Architecture
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• v.39 no.4
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• pp.99-110
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• 2021

This study intends to examine the characteristics of the construction method of the urban water system in the historical cities of Korea, focusing on Gyeongju, Gyeongju, Goryeo, Hanyang, and Suwon Hwaseong, which were created as new towns in the late Joseon Dynasty. It examines the meaning of waterways in connection with the urban skeletal structure, focusing on the location of cities, roads, and arrangement of urban facilities, and analyzes the compositional form of the water system. We tried to derive the relationship with the structure. In particular, it can be seen that water and natural water systems act as key factors in determining the location of a city, and have a close relationship with the urban structure, urban development process, and urban structure. In addition to the symbolic meaning of water in harmony with the geographical concept, realistic demands in terms of water level and water retention are an important background. In order to respond to various demands related to water space, various planning and technical elements for managing water space were introduced in the process of city formation and development. The planning elements of the urban water system in the process of urban formation and development are summarized as follows through the analysis of the research site. First, in the process of building the urban water system in Gyeongju, Goryeo, Goryeo, Hanyang, and Hwaseong, Suwon, which were selected as the research destinations, the water system in consideration of drainage and disaster is common, but the water system construction method and usability are common. shows the difference. Second, water and natural water systems act as symbolic elements to secure the legitimacy of the city location, and as a key factor in determining the location of the city in harmony with the geographical concept and determining the left direction of the city. Third, the natural water system prior to the formation of a city works as a basis for determining the compositional form of the urban water system constructed in the process of urban formation and development in harmony with the topographical conditions. Fourth, the urban water system built on the basis of natural water systems is constructed by linking natural waterways and planned artificial waterways. Fifth, the urban water system is being built in a planned manner in consideration of the utility in connection with the urban structure, such as securing of urban land, arrangement of urban facilities and areas, composition of functional areas, and land division, in addition to the perspective of drainage system and flood control in consideration of disasters.