• BMB Reports
• /
• v.34 no.1
• /
• pp.28-32
• /
• 2001

The Fos-Jun heterodimers are part of the regulatory network of gene expression and nuclear proteins encoded by proto-oncogenes. The activation of Fos-Jun is important in the transmission of the tumor-promoting signal from the extracellular environment to the nuclear transcription mechanism. To search for the inhibitors of the Fos-Jun DNA complex formation, several natural products were screened and water-soluble paeoniflorin reduced the binding activity of the Fos-Jun heterodimer. This active compound was purified by silica gel column chromatography and HPLC. The electrophoresis mobility shift assay and reverse-phase HPLC test showed that paeoniflorin reduced the AP-l function. The cytotoxic effect of paeoniflorin was observed in HL-60. These results indicate that paeoniflorin blocks the Fos-Jun heterodimer-binding site of the AP-l DNA and it also has cytotoxic effects on human leukemia cell lines.

• BMB Reports
• /
• v.32 no.5
• /
• pp.497-501
• /
• 1999

Biotinylation of recombinant proteins is a powerful tool for the detection and analysis of proteins of interest in a large variety of assay systems. The recent development of in vivo biotinylation techniques in E. coli has opened new possibilities for the production of site-specifically biotinylated proteins without the need for further manipulation after the isolation of the recombinantly expressed proteins. In the present study, a novel vector set was generated which allows the convenient cloning and expression of proteins of interest fused with an N-terminal in vivo biotinylated thioredoxin (TRX) protein. These vectors were derived from the previously reported pBIOTRX vector into which was incorporated part of the pBluescript II+phagemid multiple cloning site (MCS), amplified by PCR using a pair of sophisticated oligonucleotide primers. The functionality of these novel vectors was examined in this system by recombinant expression of rat transforming growth factor-$\beta$. Western-blot analysis using TRX-specific antibodies or peroxidase-conjugated streptavidin confirmed the successful induction of the fusion protein and the in vivo conjugation of biotin molecules, respectively. The convenience of molecular subcloning provided by the MCS and the effective in vivo biotinylation of proteins of interest makes this novel vector set an interesting alternative for the production of biotinylated proteins.

• Journal of Microbiology and Biotechnology
• /
• v.3 no.3
• /
• pp.139-145
• /
• 1993

The nucleotide sequence of the xylanase gene (xynS) from alkali-tolerant Bacillus sp. YA.14 was determined and analyzed. A 639 base pairs open reading frame for xynS gene was observed and encoded for a protein of 213 amino acids with a molecular weight of 23, 339. S1 nuclease mapping showed that the transcription initiation site of the xynS gene did not exist in the cloned DNA. Ribosome binding site sequence with the free energy of -18.8 Kcal/mol was observed 8 base pairs upstream from the initiation codon, ATG. The proposed signal sequence consisted of 28 amino acids, of which 3 were basic amino acid residues and 21 were hydrophobic amino acid residues. When the amino acid sequences of xylanases were compared, Bacillus sp. YA-14 xylanase showed 48% homology with Bacillus sp. YC-335 xylanase and 96% homology with xylanases from B. subtilis and B. circulans.

• Environmental Engineering Research
• /
• v.25 no.1
• /
• pp.29-35
• /
• 2020

The aim of this study was to estimate the microbial metabolic activity of indigenous soil microbes under the radon exposure with different intensity and times in the secured laboratory radon chamber. For this purpose, the soil microbes were collected from radon-contaminated site located in the G county, Korea. Thereafter, their metabolic activity was determined after the radon exposure of varying radon concentrations of 185, 1,400 and 14,000 Bq/㎥. The average depth variable concentrations of soil radon in the radon-contaminated site were 707, 860 and 1,185 Bq/㎥ from 0, 15, and 30 cm in deep, respectively. Simultaneously, the soil microbial culture was mainly composed of Bacillus sp., Brevibacillus sp., Lysinibacillus sp., and Paenibacillus sp. From the radon exposure test, higher or lower radiation intensities compared to the threshold level attributed the metabolic activity of mixed microbial consortium to be reduced, whereas the moderate radiation intensity (i.e. threshold level) induced it to the pinnacle point. It was decided that radon radiation could instigate the microbial metabolic activity depending on the radon levels while they were exposed, which could consequently address that the certain extent of threshold concentration present in the ecosystem relevant to microbial diversity and population density to be more proliferated.

• Proceedings of the Korean Geotechical Society Conference
• /
• 2007.09a
• /
• pp.305-316
• /
• 2007

Free field ground motion during earthquake is significantly affected by the local soil conditions and it is essential for the seismic design to perform the site specific ground response analysis. So, Round Robin Test (RRT) on ground response analysis was performed for three sites in Korea. A total of 12 teams presented the results of ground response analysis with used input soil properties based on own judgement. In this paper, the results of one dimensional equivalent linear analysis presented by 11 teams were compared to evaluate the effect of input soil properties on ground response analysis. Additionally, 4 influence factors on ground response analysis, that is shear wave velocity of soil layer, nonlinear dynamic deformational characteristics, bedrock depth and bedrock velocity were studied for assumed simple soil conditions.

• Journal of Environmental Science International
• /
• v.15 no.4
• /
• pp.359-364
• /
• 2006

Two sites with different average daily traffic volume at an intersection were selected in order to investigate water quality of the first flushes in the surface runoffs. The effects of rainfall intensities and accumulated non-rainy days before rainfall events on the water quality were also delineated. Samples were collected at every 2 min. interval from each first flush from February to May, 2004 for 4 major rainfall events. $COD_{cr}$ or SS concentrations at the site with an average daily traffic volume (ADTV) of 23,000 vehicles were 2-7 times higher than those at the site with an ADTV of 1,400 vehicles. The longer the accumulated non-rainy days were, the higher the concentration of heavy metals were than those of $COD_{cr}$ and SS in the first flushes.

• Proceedings of the Korean Geotechical Society Conference
• /
• 2005.03a
• /
• pp.171-178
• /
• 2005

It is unavoidable to be formed slopes under special circumstance of Korea where 70% of the whole area are composed of mountains when civil engineering projects such as roads, site developments are increased with industrial development and horizontal expansions of urban area. Moreover, stability of the slopes become one of quite important issues under special meteorological characteristics that over two-thirds of annual average rainfall are concentrated in summer season and the localized torrential downpour are getting more frequent recently. Resulting in these circumstances, partial slope failures by debris flow of the high water content soils are occurred frequently in cutting soil slopes. In this case of debris flow slope failure, slope declination method are selected for their stable recovery because it is impossible to recover entirely by existing recovery methods. Seeding or special grass planting methods are followed separately without exception. The method by which entire recover with bigger stability ratio would be possible and grass planting work would be done simultaneously is developed. In this study, the results of the tests including a real construction history in a failed slope using developed method are described

• BMB Reports
• /
• v.41 no.4
• /
• pp.305-309
• /
• 2008

Cobalt is an essential microelements in many biological processes involving enzymatic activity. We found that $Zn^{2+}$ and $Mg^{2+}$, which are in the active site of native calf intestine alkaline phosphatase (CIP), can be replaced by $Co^{2+}$ directly in solution. The effect of $Co^{2+}$ concentration on the substitution reaction was examined at ratios of [$Co^{2+}$]/[CIP] from 0:1 to 8:1. The quantity of $Zn^{2+}$ in CIP decreased progressively as the ratio was increased, but the amount of $Mg^{2+}$ changed in irrregular fashion. A series of active site models of the reaction mechanism of CIP are proposed. Low pH was found to promote the replacement of $Mg^{2+}$ by $Co^{2+}$. To understand how the substitution affects the enzyme, we also solved the secondary structure of CIP after reaction with $Co^{2+}$ in different conditions.

• Journal of Integrative Natural Science
• /
• v.11 no.2
• /
• pp.121-129
• /
• 2018

CXCR6 is a major target in drug design as it is a determinant receptor in many diseases like AIDS, Type I Diabetes, some cancer types, atherosclerosis, tumor formation, liver disease and steatohepatitis. In this study, we propose the active site residues of CXCR6 molecule. We employed homology modelling and molecular docking approach to generate the 3D structure for CXCR6 and to explore its interaction between the antagonists and agonists. 3D models were generated using 14 different templates having high sequence identity with CXCR6. Surflex docking studies using pyridine and pyrimidine derivatives enabled the analysis of the binding site and finding of the important residues involved in binding. 3D structure of CXCL16, a natural ligand for CXCR6, was modelled using PHYRE and protein - protein docking was performed using ClusPro. The residues which were found to be crucial in interaction with the ligand are THR110, PHE113, TYR114, GLN160, GLN195, CYS251 and SER255. This study can be used as a guide for therapeutic studies of human CXCR6.

• Journal of the Korean Society of Safety
• /
• v.15 no.4
• /
• pp.112-118
• /
• 2000

This paper is resulted from the research to activate Self-control Safety Management system that adopted to improve an assessment system for Hazard Prevention Plan. And members of company, university and research institute have jointly participated in the research. First, it is investigated that introduced background and processing method with existing practical data & materials references in order to understand what domestic Self-control Safety Management system will be available for. And general construction company at site have their ability to assess Hazard Prevention Plan by mailing questionnaire to on thousand site, visits, and interviews. Also, It is investigated how much they have the assessment ability. It is selected to do a questionnaire survey for the status of self-regulatory safety assessment ability of the designated self-regulatory companies in order to produce and enhance the self-regulatory assessment ability and the necessity of Self-control Safety Management system. Finally, it is selected to do a questionnaire survey for fixing and the enhance Self-control Safety Management system of general construction contractors.