• Title/Summary/Keyword: engineering site

Search Result 7,646, Processing Time 0.042 seconds

The Growth of Human Osteoblasts in Culture Dishes Made with Poly-glycolic Acid Containing Fetal Bovine Serum (우태아 혈청이 포함된 Poly-glycolic Acid 배양판에서 인간 조골세포의 성장)

  • Choi, Jae Won;Kim, Yong Ha;Moon, Young Mi;Kim, Yoen Jung;Choi, Sik Young
    • Archives of Plastic Surgery
    • /
    • v.33 no.5
    • /
    • pp.612-615
    • /
    • 2006
  • Purpose: An ideal bony construct can be divided into two broad categories: (1) the design and fabrication of biodegradable, biomimetic scaffolds that provide correct signals to induce osteogenesis: (2) the identification of an ideal source of osteoprogenitor cells to seed onto the scaffold. We selected poly-glycolic acid as a synthetic scaffold among various scaffolds because of these properties. Meanwhile, culture medium is supplemented with fetal bovine serum(FBS): such serum contains essential elements such as proteins, hormones, growth factors and trace minerals. The composition of FBS can be ideal for various cell growth in vitro. We supposed that we could enhance bone growth at a fractured site if FBS was mixed with synthetic scaffold-PGA. Methods: We cultured human osteoblasts in five different prepared culture dishes made with FBS and PGA mixture. The mixtures contained different ratio of FBS, that is, 0, 1.5, 3, 7, and 10%. We cultured human osteoblasts for seven days and examined the growth and attachment of the cells at the 1st, 3rd, 5th, 7th days, respectively. Results: In the mixture of 0% FBS and PGA, the growth of the cells lasted for one day. In 1.5 and 3% FBS and PGA, the growth of the cells was examined at the 3rd day, then minimally declined at the 5th and 7th days. In 7% FBS and PGA, the growth of the cells lasted for 5 days, then declined at the 7th day. In 10% FBS and PGA, the growth of the cells lasted for 5 days, then declined at the 7th day. Staining status of the osteoblasts with alkaline phosphatase showed pale pink color in 0% FBS and PGA groups, but bright pink color in 1.5, 3, 7, 10% FBS and PGA groups, especially in 3%, 7%. Conclusion: In consequence, the growth of human osteoblast was higher in the mixture of FBS and PGA groups than in pure PGA ones. It is assumed that the mixture of FBS and PGA affects the proliferation of human osteoblasts.

Formation of an Intestine-Cartilage Composite Graft for Tracheal Reconstruction (기관 재건을 위한 장과 연골의 복합 이식판 개발)

  • Jheon, Sang-Hoon;Lee, Sub;Jung, Jin-Yong;Kong, Jun-Hyuk;Lim, Jeong-Ok;Kim, Yu-Mi;Jin, Chun-Jin;Park, Tae-In;Lee, jae-Ik;Sung, Seok-Whan;Choh, Joong-Haeng
    • Journal of Chest Surgery
    • /
    • v.37 no.6
    • /
    • pp.474-481
    • /
    • 2004
  • Background: Tracheal transplantation is necessary in patients with extensive tracheal stenosis, congenital lesions and other oncologic conditions but bears. many critical problems compared to other organ transplantations. The purpose of this study was to develop intestine-cartilage composite grafts for potential application in tracheal reconstruction by free intestinal graft. Material and Method: Hyaline cartilage was harvested from trachea of 2 weeks old New Zealand White Rabbits. Chondrocytes were isolated and cultured for 8 weeks. Cultured chondrocytes were seeded in the PLGA scaffolds and mixed in pluronic gel Chondrocyte bearing scaffolds and gel mixture were embedded in submucosal area of stomach and colon of 3 kg weighted New Zealand White Rabbits under general anesthesia. 10 weeks after implantation, bowels were harvested for evaluation. Result: We identified implantation site by gross examination and palpation. Developed cartilage made a good frame for shape memory. Microscopic examinations included special stain s howed absorption of scaffold and cartilage formation even though it was not fully matured. Conclusion: Intestine-cartilage composite graft could be applicable in the future as tracheal substitute and should be further investigated.

Research and Policy Directions against Ambient Fine Particles (초미세먼지 문제 해결을 위한 연구 및 정책 방향)

  • Kim, Yong Pyo
    • Journal of Korean Society for Atmospheric Environment
    • /
    • v.33 no.3
    • /
    • pp.191-204
    • /
    • 2017
  • Concerns on the air pollution problem caused by ambient fine particles have become a big social issue in Korea. Important factors that should be addressed to develop effective and efficient air quality management policy, especially, against fine particles are discussed and research and policy directions to address these factors are suggested. It is suggested that two factors are in high priority; one is scientific understanding of the major formation mechanisms of fine particles and the other is the process of policy decision and implementation. For the scientific understanding, smog chamber measurement, intensive field study, and chemical transport model development that can simulate the characteristics of Northeast Asia are considered to be important. For the policy directions, priority setting of the proposed policies and development and implement of effective communication sytem are considered to be important.

Using Google Earth for a Dynamic Display of Future Climate Change and Its Potential Impacts in the Korean Peninsula (한반도 기후변화의 시각적 표현을 위한 Google Earth 활용)

  • Yoon, Kyung-Dahm;Chung, U-Ran;Yun, Jin-I.
    • Korean Journal of Agricultural and Forest Meteorology
    • /
    • v.8 no.4
    • /
    • pp.275-278
    • /
    • 2006
  • Google Earth enables people to easily find information linked to geographical locations. Google Earth consists of a collection of zoomable satellite images laid over a 3-D Earth model and any geographically referenced information can be uploaded to the Web and then downloaded directly into Google Earth. This can be achieved by encoding in Google's open file format, KML (Keyhole Markup Language), where it is visible as a new layer superimposed on the satellite images. We used KML to create and share fine resolution gridded temperature data projected to 3 climatological normal years between 2011-2100 to visualize the site-specific warming and the resultant earlier blooming of spring flowers over the Korean Peninsula. Gridded temperature and phonology data were initially prepared in ArcGIS GRID format and converted to image files (.png), which can be loaded as new layers on Google Earth. We used a high resolution LCD monitor with a 2,560 by 1,600 resolution driven by a dual link DVI card to facilitate visual effects during the demonstration.

Performance of Northern Exposure Index in Reducing Estimation Error for Daily Maximum Temperature over a Rugged Terrain (북향개방지수가 복잡지형의 일 최고기온 추정오차 저감에 미치는 영향)

  • Chung, U-Ran;Lee, Kwang-Hoe;Yun, Jin-I.
    • Korean Journal of Agricultural and Forest Meteorology
    • /
    • v.9 no.3
    • /
    • pp.195-202
    • /
    • 2007
  • The normalized difference in incident solar energy between a target surface and a level surface (overheating index, OHI) is useful in eliminating estimation error of site-specific maximum temperature in complex terrain. Due to the complexity in its calculation, however, an empirical proxy variable called northern exposure index (NEI) which combines slope and aspect has been used to estimate OHI based on empirical relationships between the two. An experiment with real-world landscape and temperature data was carried out to evaluate performance of the NEI - derived OHI (N-OHI) in reduction of spatial interpolation error for daily maximum temperature compared with that by the original OHI. We collected daily maximum temperature data from 7 sites in a mountainous watershed with a $149 km^2$ area and a 795m elevation range ($651{\sim}1,445m$) in Pyongchang, Kangwon province. Northern exposure index was calculated for the entire 166,050 grid cells constituting the watershed based on a 30-m digital elevation model. Daily OHI was calculated for the same watershed ana regressed to the variation of NEI. The regression equations were used to estimate N-OHI for 15th of each month. Deviations in daily maximum temperature at 7 sites from those measured at the nearby synoptic station were calculated from June 2006 to February 2007 and regressed to the N-OHI. The same procedure was repeated with the original OHI values. The ratio sum of square errors contributable by the N-OHI were 0.46 (winter), 0.24 (fall), and 0.01 (summer), while those by the original OHI were 0.52, 0.37 and 0.15, respectively.

An Empirical Study of Hot Water Supply Patterns and Peak Time in Apartment Housing with District Heating System (공동주택의 급탕부하 지속시간 및 부하 패턴에 관한 실증연구)

  • Kim, Sung-Min;Chung, Kwang-Seop;Kim, Young-Il
    • Journal of Energy Engineering
    • /
    • v.21 no.4
    • /
    • pp.435-443
    • /
    • 2012
  • The combination of space shortage and the high population density concentrated in urban areas of South Korea has resulted in the growth of large-scale high-rise residential complexes, naturally affecting water and hot water usage patterns as well. But the current designs for water and hot water supply in South Korea rely mostly on international design standards and data calculated on site due to the severe shortage of basic data in relation to actual use, which result in the frequent problem of the under-or over-design of water and hot water supply. The following study measures the hot water supplier's conditions and the user's heat usage to realize the amount of time required for hot water supply load generation and the pattern of actual use in order to create basic data for effective hot water supply facility design and maintenance.

Analysis of Temperature Influence Experiment on Green Spaces in Campus (캠퍼스 내 녹지공간의 온도분석 및 온도영향요인 규명 실험)

  • Kim, Jaekyoung;Kim, Wonhee;Kim, Eunil
    • Journal of the Korea Academia-Industrial cooperation Society
    • /
    • v.21 no.2
    • /
    • pp.511-520
    • /
    • 2020
  • Owing to global warming, heat waves have become stronger in the summer, and research on improving the thermal environment of green spaces, such as urban parks, is being conducted. On the other hand, studies on improving the urban thermal environment, which is changing due to the greening pattern and the intensity of the wind, are still insufficient. This study analyzed the temperature of the green spaces on campus to understand the factors affecting the temperature changes. After investigating the covering condition and planting form of the site, factors, such as temperature, humidity, wind direction, wind speed, and illuminance, were measured. The most influential factors on the temperature distribution are evapotranspiration and wind - induced heat transfer. The other major factors affecting the temperature change were the type of cover, wind velocity/wind direction, type of planting, shade / solar irradiance. In the type of cover, the plant was classified as low temperature, and the asphalt pavement was classified as high temperature. In wind speed, instantaneous temperature was reduced by 1.2 ℃ in southern wind, 0.7 ℃ in the westerly wind, 0.4 ℃ in the north wind and 0.5 ℃ in the east wind when a wind of 3.5m/s or more was blown.

Regional Optimization of Food Waste Management - Focused on Three Cities in Kyungnam Province - (음식물쓰레기 관리의 광역적 최적화 방안 - 경남 3개 도시를 중심으로 -)

  • Lee, Myung-Hi;Park, Jung-Seok;Kin, Jong-Oh
    • Journal of the Korea Organic Resources Recycling Association
    • /
    • v.7 no.1
    • /
    • pp.53-66
    • /
    • 1999
  • Limited landfill area and finance as well as a large generation of food wastes, have called for systematic approaches towards potential cost savings. In this study, the regional management system of food wastes generated from the residential and commercial sources was considered in three cities bounded each other; Changwon, Masan, and Jinhae city. Total thirteen alternatives were established and compared by applying the WRAP (Waste Resource Allocation Program). The following results were obtained: 1. While relatively small amounts were generated from the commercial sources such as cafeteria, restaurants, and market facilities, almost 80% of food wastes were generated from the residential sources. 2. Unit costs for food waste management in three cities were different according to their present situation such as the type and location of major generation sources and treatment facilities. Especially, the highest cost appeared in Jinhae city due to the most expensive construction of coastal landfill site. 3. Considering proper revenue, the whole conversion of food waste into animal feed was selected as the optimal alternative and represented 60% to 74% of the management cost of the whole landfill alternative in all cities. Comparing the other alternatives, composting of food waste was more economical than landfill alternative and the incineration was the most expensive alternative. 4. Some of the regional management systems using common food waste processing facilities together in three cities showed to be more economical than the single management system. Therefore, more detailed research for the regional management systems of food waste was recommended.

  • PDF

Stochastic numerical study on the propagation characteristics of P-Wave in heterogeneous ground (지반의 비균질성이 탄성파 전파 특성에 미치는 영향에 대한 추계론적 수치해석 연구)

  • Song, Ki-Il
    • Journal of Korean Tunnelling and Underground Space Association
    • /
    • v.15 no.1
    • /
    • pp.13-24
    • /
    • 2013
  • Various elastic wave-based site investigation methods have been used to characterize subsurface ground because the dynamic properties can be correlated with various geotechnical parameters. Although the inherent spatial variability of the geotechnical parameters affects the P-wave propagation characteristics, ground heterogeneity has not been considered as an influential factor. Thus, the effect of heterogeneous ground on the travel-time shift and wavefront characteristics of elastic waves through stochastic numerical analyses is investigated in this study. The effects of the relative correlation lengths and relative propagation distances on the travel-time shift of P-waves considering various intensities of ground heterogeneity were investigated. Heterogeneous ground fields of stiffness (e.g., the coefficient of variation = 10 ~ 40%) were repeatedly realized in numerical finite difference grids using the turning band method. Monte Carlo simulations were undertaken to simulate P-wave propagation in heterogeneous ground using a finite difference method-based numerical approach. The results show that the disturbance of the wavefront becomes more significant with stronger heterogeneity and induces travel-time delays. The relative correlation lengths and propagation distances are systematically related to the travel-time shift.

Impacts of Combined Hydrogeological and Chemical Heterogeneities on the Transport of Leachate through Landfill Sites (수리지질학적, 화학적 특성의 복합 불균질성이 매립지반 내 침출수 이동에 미치는 영향)

  • Lee, Kun-Sang
    • Journal of Korean Society of Environmental Engineers
    • /
    • v.31 no.4
    • /
    • pp.300-307
    • /
    • 2009
  • The transport of landfill leachate in the subsurface formations of unlined landfill sites is considered. The impacts of hydrogeological and chemical heterogeneities on the leachate transport are assessed by examining the results from a series of Monte-Carlo simulations. The landfill system simulated in this study is hypothetically represented with three levels of spatial variability for the hydrogeological and chemical parameter; (1) homogeneous hydraulic conductivity (K) and distribution coefficient ($K_d$), (2) K heterogeneity only, and (3) combined heterogeneities of K and $K_d$. To calculate the transport of leachate through negatively-correlated random hypothetical K-$K_d$ fields generated using geostatistical input parameters, a saturated flow model is linked with a contaminant transport model. Point statistic values such as mean, standard deviation, and coefficient of variation of the concentration were obtained from 100 Monte-Carlo trials. Results of point statistics show that the heterogeneities of K and $K_d$ in the landfill site prove to be an important parameter in controlling leachate concentrations. Consideration of combined K and $K_d$ heterogeneities results in enhancing the variability of contaminant transport. The variability in the leachate concentration for different realizations also increases as the distance between source and monitoring well increase.