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Conserved Genes and Metabolic Pathways in Prokaryotes of the Same Genus (동일한 속 원핵생물들의 보존 유전자와 대사경로)

  • Lee, Dong-Geun;Lee, Sang-Hyeon
    • Journal of Life Science
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    • v.29 no.1
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    • pp.123-128
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    • 2019
  • The use of 16S rDNA is commonplace in the determination of prokaryotic species. However, it has limitations, and there are few studies at the genus level. We investigated conserved genes and metabolic pathways at the genus level in 28 strains of 13 genera of prokaryotes using the COG database (conserved genes) and MetaCyc database (metabolic pathways). Conserved genes compared to total genes (core genome) at the genus level ranged from 27.62%(Nostoc genus) to 71.76%(Spiribacter genus), with an average of 46.72%. The lower ratio of core genome meant the higher ratio of peculiar genes of a prokaryote, namely specific biological activities or the habitat may be varied. The ratio of common metabolic pathways at the genus level was higher than the ratio of core genomes, from 58.79% (Clostridium genus) to 96.31%(Mycoplasma genus), with an average of 75.86%. When compared among other genera, members of the same genus were positioned in the closest nodes to each other. Interestingly, Bacillus and Clostridium genera were positioned in closer nodes than those of the other genera. Archaebacterial genera were grouped together in the ortholog and metabolic pathway nodes in a phylogenetic tree. The genera Granulicella, Nostoc, and Bradyrhizobium of the Acidobacteria, Cyanobacteria, and Proteobacteria phyla, respectively, were grouped in an ortholog content tree. The results of this study can be used for (i) the identification of common genes and metabolic pathways at each phylogenetic level and (ii) the improvement of strains through horizontal gene transfer or site-directed mutagenesis.

Review of Quantification of Fracture Characteristics Based on Topological Analysis (위상기하 분석법을 이용한 단열계 특성 정량화의 소개)

  • Son, Hyorok;Kim, Young-Seog
    • The Journal of Engineering Geology
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    • v.31 no.1
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    • pp.1-17
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    • 2021
  • It is important to evaluate the fracture network in a rock volume because fractures control the ground conditions and fluid flow characteristics. Therefore, various attempts have been made to quantify fracture networks to better understand ground and flow conditions. The use of fracture density alone (a quantitative parameter based on geometric analysis) does not fully explain the evolution of fracture networks, or quantify the spatial relationship (e.g. connectivity) of fractures in a rock mass. Therefore, the need for fracture network characterization based on topological analysis has recently emerged. In Korea however, the topological analysis of fracture networks within a rock mass has rarely been studied. As such, the definition of the topological analysis of fracture networks and the graph theory related to the topological analysis are briefly summarized in this study. We also introduce an application method for these analyses to fracture characterization. If the topological method is used for the analysis of fracture networks, it can also be adopted to analyze fluid flow characteristics of groundwater, characterize petroleum reservoirs, and analyze the evolution of a fracture network. In addition, topological analysis can be useful for site selection of major facilities such as nuclear waste disposal sites because it can be used to evaluate the stability of the potential sites.

Pollution characteristics of PM2.5 observed during January 2018 in Gwangju (광주 지역에서 2018년 1월 측정한 초미세먼지의 오염 특성)

  • Yu, Geun-Hye;Park, Seung-Shik;Jung, Sun A;Jo, Mi Ra;Jang, Yu Woon;Lim, Yong Jae;Ghim, Young Sung
    • Particle and aerosol research
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    • v.15 no.3
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    • pp.91-104
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    • 2019
  • In this study, hourly measurements of $PM_{2.5}$ and its major chemical constituents such as organic and elemental carbon (OC and EC), and ionic species were made between January 15 and February 10, 2018 at the air pollution intensive monitering station in Gwangju. In addition, 24-hr integrated $PM_{2.5}$ samples were collected at the same site and analyzed for OC, EC, water-soluble OC (WSOC), humic-like substance (HULIS), and ionic species. Over the whole study period, the organic aerosols (=$1.6{\times}OC$) and $NO_3{^-}$ concentrations contributed 26.6% and 21.0% to $PM_{2.5}$, respectively. OC and EC concentrations were mainly attributed to traffic emissions with some contribution from biomass burning emissions. Moreover, strong correlations of OC with WSOC, HULIS, and $NO_3{^-}$ suggest that some of the organic aerosols were likely formed through atmospheric oxidation processes of hydrocarbon compounds from traffic emissions. For the period between January 18 and 22 when $PM_{2.5}$ pollution episode occurred, concentrations of three secondary ionic species ($=SO{_4}^{2-}+NO_3{^-}+NH_4{^+}$) and organic matter contributed on average 50.8 and 20.1% of $PM_{2.5}$, respectively, with the highest contribution from $NO_3{^-}$. Synoptic charts, air mass backward trajectories, and local meteorological conditions supported that high $PM_{2.5}$ pollution was resulted from long-range transport of haze particles lingering over northeastern China, accumulation of local emissions, and local production of secondary aerosols. During the $PM_{2.5}$ pollution episode, enhanced $SO{_4}^{2-}$ was more due to the long-range transport of aerosol particles from China rather than local secondary production from $SO_2$. Increasing rate in $NO_3{^-}$ was substantially greater than $NO_2$ and $SO{_4}^{2-}$ increasing rates, suggesting that the increased concentration of $NO_3{^-}$ during the pollution episode was attributed to enhanced formation of local $NO_3{^-}$ through heterogenous reactions of $NO_2$, rather than impact by long-range transportation from China.

Correction Algorithm of Errors by Seagrasses in Coastal Bathymetry Surveying Using Drone and HD Camera (드론과 HD 카메라를 이용한 수심측량시 잘피에 의한 오차제거 알고리즘)

  • Kim, Gyeongyeop;Choi, Gunhwan;Ahn, Kyungmo
    • Journal of Korean Society of Coastal and Ocean Engineers
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    • v.32 no.6
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    • pp.553-560
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    • 2020
  • This paper presents an algorithm for identifying and eliminating errors by seagrasses in coastal bathymetry surveying using drone and HD camera. Survey errors due to seagrasses were identified, segmentated and eliminated using a L∗a∗b color space model. Bathymetry survey using a drone and HD camera has many advantages over conventional survey methods such as ship-board acoustic sounder or manual level survey which are time consuming and expensive. However, errors caused by sea bed reflectance due to seagrasses habitat hamper the development of new surveying tool. Seagrasses are the flowering plants which start to grow in November and flourish to maximum density until April in Korea. We developed a new algorithm for identifying seagrasses habitat locations and eliminating errors due to seagrasses to get the accurate depth survey data. We tested our algorithm at Wolpo beach. Bathymetry survey data which were obtained using a drone with HD camera and calibrated to eliminate errors due to seagrasses, were compared with depth survey data obtained using ship-board multi-beam acoustic sounder. The abnormal bathymetry data which are defined as the excess of 1.5 times of a standard deviation of random errors, are composed of 8.6% of the test site of area of 200 m by 300 m. By applying the developed algorithm, 92% of abnnormal bathymetry data were successfully eliminated and 33% of RMS errors were reduced.

Accuracy Assessment of Aerial Triangulation of Network RTK UAV (네트워크 RTK 무인기의 항공삼각측량 정확도 평가)

  • Han, Soohee;Hong, Chang-Ki
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.38 no.6
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    • pp.663-670
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    • 2020
  • In the present study, we assessed the accuracy of aerial triangulation using a UAV (Unmanned Aerial Vehicle) capable of network RTK (Real-Time Kinematic) survey in a disaster situation that may occur in a semi-urban area mixed with buildings. For a reliable survey of check points, they were installed on the roofs of buildings, and static GNSS (Global Navigation Satellite System) survey was conducted for more than four hours. For objective accuracy assessment, coded aerial targets were installed on the check points to be automatically recognized by software. At the instance of image acquisition, the 3D coordinates of the UAV camera were measured using VRS (Virtual Reference Station) method, as a kind of network RTK survey, and the 3-axial angles were achieved using IMU (Inertial Measurement Unit) and gimbal rotation measurement. As a result of estimation and update of the interior and exterior orientation parameters using Agisoft Metashape, the 3D RMSE (Root Mean Square Error) of aerial triangulation ranged from 0.153 m to 0.102 m according to the combination of the image overlap and the angle of the image acquisition. To get higher aerial triangulation accuracy, it was proved to be effective to incorporate oblique images, though it is common to increase the overlap of vertical images. Therefore, to conduct a UAV mapping in an urgent disaster site, it is necessary to acquire oblique images together rather than improving image overlap.

Estimation of SO2 emissions in large point sources at Dangjin City using airborne measurements (항공관측 결과를 활용한 당진시 대형사업장에서의 황산화물 배출량 평가)

  • Kim, Yong Pyo;Kim, Saewung;Kim, Jongho;Lee, Taehyoung
    • Particle and aerosol research
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    • v.16 no.4
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    • pp.107-117
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    • 2020
  • Based on the airborne measurement results over a coal fired power plant and steel work in Dangjin city, SO2 emission amounts of each site are estimated (top-down emission). Airborne measurements were carried out on May-June and October-November 2019. The estimated SO2 emission in 2019 for the power plant was 1502.1 kg/hr and that for the steel work was 2850.5 kg/hr, higher as much as a factor of 2.5 and 2.0, respectively, than the emission amounts provided by both facilities (bottom-up emission). The outcomes strongly illustrates that well designed airborne observations can serve a quantitative diagnostic tool for bottom-up emission estimates. Further research direction to improve the reliability of the top-down emission estimates is suggested.

A Pilot Study on Installation Criteria of Speed Change Lanes according to the Implementation of Safety Speed "5030" in the Urban Area (도심내 안전속도 "5030"시행에 따른 변속차로 설치기준에 관한 기초연구)

  • Oh, Seok-Jin;Ha, Tae-Jun;Jin, Tae-Hee;Shin, Kwang-Min;Lee, Gang-Seok
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.41 no.1
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    • pp.57-64
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    • 2021
  • The speed change lane is installed at the point where the roads are connected on the urban roads, reducing the relative speed to facilitate traffic communication. However, in the case of the speed limit of 30 km/h in accordance with the implementation of the safe speed "5030" in the city, the standard is ambiguous, and there are criticisms about the installation of the transmission lane. This study conducted a pilot study on the traffic patterns of the speed change lanes, focusing on the section where the safe speed "5030" policy was scheduled to be implemented after theoretical consideration of the speed change lanes. After on-site investigation, as a result of analyzing the traffic pattern according to the travel speed of the main road according to road conditions and traffic conditions, the effectiveness of the speed change lane according to the safety speed "5030" was analyzed. In this study, the installation criteria for speed change lane by speed were presented. The criteria is that "speed change lanes should not be installed when the speed limit is 30 km/h, and speed change lanes should be installed when the speed limit is 50 km/h." The criteria will be considered as a reference when establishing engineering standards for connection of other facilities in urban areas in the future. It is expected that unnecessary restrictions on individuals' exercise of private property rights will be minimized, smooth traffic flow and secure safety by the installation of speed change lanes and expansion of turning radius.

Causal Loop Diagram for the Relation Between Degree of Field Safety Management and Productivity Based on Effectiveness Metrics : Focusing on Plumbing work in Mega Project (작업효율 지표 기반 안전관리행위 - 생산성 인과 모델 구축 - 메가 프로젝트 배관공사 중심으로 -)

  • Chae, Yeon;Park, Moonseo;Cho, Jongwoo;Lee, Jinsol;Joo, Seonu;Hong, Yeongmin
    • Korean Journal of Construction Engineering and Management
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    • v.22 no.2
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    • pp.12-20
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    • 2021
  • Unsafe working environments slow down working speed in that it takes a lot of time to respond in case of an accident, greatly reducing the productivity of construction work. Therefore, proper safety management that does not impede the productivity of construction works is a very important factor for successful construction project. However especially in mega project, the mutual effects of safety management and productivity are complex. Therefore, this study establishes a causal model between safety management and productivity influencing factors based on effectiveness metrics, and analyzes the effect of increased site congestion due to excessive personnel input, irregular safety measures and inefficient change of work schedules. The result of this study, is expected to contribute to enhancing the competitiveness of the construction industry as the basis for establishing a safety management plan that can secure appropriate productivity.

Feasibility of Activated-Carbon Adsorbent to Sequester Sunken Hazardous and Noxious Substances (HNS) (흡착제를 이용한 침강 HNS 처리 및 현장적용 가능성 연구 - 현장 처리를 위한 활성탄소 활용 조건 검토 및 제안 -)

  • Choi, Ki-young;Kim, Chang-joon;Kim, Hye-eun;Jung, Jun-mo;Hwang, Ho-jin;Lee, Moonjin
    • Journal of the Korean Society of Marine Environment & Safety
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    • v.26 no.7
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    • pp.858-863
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    • 2020
  • We experimented with the particle-settling velocity and CHCl3 absorption efficiency of seven activated-carbon and analyzed seven heavy metal contents by elution for application to the field treatment of sunken HNS on the marine seabed. The mean particle-settling velocity was in the range 0.5-8 cm/s, except when the 8-20 mesh was used. The larger the HNS particle, the faster the particle-settling velocity was, and the CHCl3 absorption efficiency increased considerably owing to the larger surface area. In addition, the elution test results showed that the total Zn and As contents in >100-meshed activated carbon was higher than the contents criteria for the standard for water-treatment agents, and Cr, Zn, and As were released at higher concentrations than those released by other activated-carbon groups. Taken together, the CHCl3 absorption efficiency, settling velocity, and elution test results suggested that the 20-60, 20-40, and 2mm&down mesh activated-carbon adsorbents could be applied to the field treatment of HNSs and that the minimum required amount for field treatment were 0.82, 0.90, and 1.28 ton/㎘, respectively, as calculated based on the HNS-adsorption-capacity priority.

A Study on Development of Portable Concrete Crack Measurement Device Using Image Processing Technique and Laser Sensors (이미지 처리기법 및 레이저 센서를 이용한 휴대용 콘크리트 균열 측정 장치 개발에 관한 연구)

  • Seo, Seunghwan;Ohn, Syng-Yup;Kim, Dong-Hyun;Kwak, Kiseok;Chung, Moonkyung
    • Journal of the Korean Geosynthetics Society
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    • v.19 no.4
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    • pp.41-50
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    • 2020
  • Since cracks in concrete structures expedite corrosion of reinforced concrete over a long period of time, regular on-site inspections are essential to ensure structural usability and prevent degradation. Most of the safety inspections of facilities rely on visual inspection with naked eye, so cost and time consuming are severe, and the reliability of results differs depending on the inspector. In this study, a portable measuring device that can be used for safety diagnosis and maintenance was developed as a device that measures the width and length of concrete cracks through image analysis of cracks photographed with a camera. This device captures the cracks found within a close distance (3 m), and accurately calculates the unit pixel size by laser distance measurement, and automatically calculates the crack length and width with the image processing algorithm developed in this study. In measurement results using the crack image applied to the experiment, the measurement of the length of a 0.3 mm crack within a distance of 3 m was possible with a range of about 10% error. The crack width showed a tendency to be overestimated by detecting surrounding pixels due to vibration and blurring effect during the binarization process, but it could be effectively corrected by applying the crack width reduction function.