• Journal of Microbiology and Biotechnology
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• v.16 no.6
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• pp.974-978
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• 2006

The cytochrome P450 monooxygenase from the pikromycin biosynthetic gene cluster in Streptomyces venezuelae, known as PikC, was observed to hydroxylate the unnatural substrate indole to indigo. Furthermore, the site-directed mutagenesis of PikC monooxygenase led to the mutant enzyme F171Q, in which Phe171 was altered to Gln, with enhanced activity for the hydroxylation of indole. From enzyme kinetic studies, F171Q showed an approximately five-fold higher catalytic efficiency compared with the wild-type PikC. Therefore, these results demonstrate the promising application of P450s originating from Streptomyces, normally involved in polyketide biosynthesis, to generate a diverse array of other industrially useful compounds.

• Proceedings of the Membrane Society of Korea Conference
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• 1995.09a
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• pp.57-71
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• 1995

A simple mathematical model for gacilitated mass transport with a fixed site carrier membrane was derived by assuming an instantaneous, microscopic concentration (activity) fluctuation, The concentration fluctuation, developed due to reversible chemical reaction between carrier and solute, could acuse the higher chemical potential gradient and the facilitated transport. For mathematical formulation, an analogy was employed between the mass transfer for the facilitated transport with fixed site carrier membrane and the electron transfer in a parallel resistor-capacitor (RC) circuit. For the single RC model, it was assumed that a single capacitor represented the total carrier and a solute could not inter-diffuse between matrix and carrier, allowing only two diffusional pathways, This assumption was relaxed by adopting a serial combination of the parallel RC circuit. Here, a solute diffuses in two elements (matrix or carrier) can exchange its pathway, exhibiting four diffusional pathways. The current models were examined against experimental data and the agreement was exceptional.

• Proceedings of the Korean Geotechical Society Conference
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• 2010.03a
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• pp.457-467
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• 2010

The settlement prediction is very important to preloading method for a construction site on a soft ground. At the design stage, however, it is hard to predict the settlement exactly due to limitations of the site survey. Most of the settlement prediction is performed by a regression settlement curve based on the field data during a construction. In Korea, hyperbolic method has been most commonly used to align the settlement curve with the field data, because of its simplicity and many application cases. The results from hyperbolic method, however, may be differed by data selections or data fitting methods. In this study, the analyses using hyperbolic method were performed about the field data of $\bigcirc\bigcirc$ site in Pusan. Two data fitting methods, using an axis transformation or an alternative method, were applied with the various data group. If data was used only after the ground water level being stabilized, fitting results using both methods were in good agreement with the measured data. Without the information about the ground water level, the alternative method gives better results with the field data than the method using an axis transformation.

• Proceedings of the Korean Geotechical Society Conference
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• 2007.09a
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• pp.611-622
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• 2007

In the design and construction of the coastal/offshore structures, it is very important to evaluate the geotechnical characteristics of marine soils, which support the structures. Although the offshore site investigation is much more difficult than onshore, safe and precise jobs have not been accomplished in Korea because of the insufficiency of the test equipments especially for the site with deep water depth. The main objective of this study is to develop a new type of marine cone penetration testing(CPT) system, which can be utilized to even deep sea and high depth of soil layer. The system is one of seabed types and employs the conventional cone, which shows more reliable results than miniature cone. The most important parts of the marine CPT including continuous rod system, cone penetration system with wheel drive, automatic cone rod assembly/dissembly system etc., were designed and manufactured. Some tests to verify the developed marine CPT system were performed at both onshore and offshore sites as well as mechanical test in laboratory. The test results show the consistent and promising performance of the new equipment, and thereafter the system would be applicable to various sites with practical/economical advantages.

• Journal of the Earthquake Engineering Society of Korea
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• v.17 no.5
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• pp.209-217
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• 2013

In order to verify the reliability of numerical site response analysis program, both soil free-field and base rock input motions should be provided. Beside the field earthquake motion records, the most effective testing method for obtaining the above motions is the dynamic geotechnical centrifuge test. However, need is to verify if the motion recorded at the base of the soil model container in the centrifuge facility is the true base rock input motion or not. In this paper, the appropriate input motion measurement method for the verification of seismic response analysis is examined by dynamic geotechnical centrifuge test and using three-dimensional finite difference analysis results. From the results, it appears that the ESB (equivalent shear beam) model container distorts downward the propagating wave with larger magnitude of centrifugal acceleration and base rock input motion. Thus, the distortion makes the measurement of the base rock outcrop motion difficult which is essential for extracting the base rock incident motion. However, the base rock outcrop motion generated by using deconvolution method is free from the distortion effect of centrifugal acceleration.

• Nuclear Engineering and Technology
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• v.44 no.7
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• pp.745-754
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• 2012

The correlations between Large Early Release Frequency (LERF) and Early Fatality need to be investigated for risk-informed application and regulation. In Regulatory Guide (RG) -1.174, while there are decision-making criteria using the measures of Core Damage Frequency (CDF) and LERF, there are no specific criteria on LERF. Since there are both huge uncertainties and large costs needed in off-site consequence calculation, a LERF assessment methodology needs to be developed, and its correlation factor needs to be identified, for risk-informed decision-making. A new method for estimating off-site consequence has been presented and performed for assessing health effects caused by radioisotopes released from severe accidents of nuclear power plants in this study. The MACCS2 code is used for validating the source term quantitatively regarding health effects, depending on the release characteristics of radioisotopes during severe accidents. This study developed a method for identifying correlations between LERF and Early Fatality and validates the results of the model using the MACCS2 code. The results of this study may contribute to defining LERF and finding a measure for risk-informed regulations and risk-informed decision-making.

• Journal of Environmental Science International
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• v.19 no.7
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• pp.819-827
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• 2010

This study was conducted to investigate runoff characteristics of non-point pollutants source at the urban area in boeun area, Chungbuk Province. The monitoring site covering the watershed of $2.11\;km^2$ contains about 40.3 % of total watershed with the urban area. The monitoring was conducted with six events for five months and Event Mean Concentration(EMC) and Site Mean Concentration(SMC) of SS, BOD, CODMn, T-N, T-P were calculated on the result of the water quality parameters. As a result of the comparion between Arithmetic Mean Concentration and Event Mean Concentration, it showed that over all Event Mean Concentration was higher than Arithmetic Mean Concentration. And it showed that SS, BOD, T-P featured the first-flushing effect, showing relatively high concentration in early-stage storm event.

• Journal of Microbiology and Biotechnology
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• v.22 no.7
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• pp.917-922
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• 2012

Homology modeling of Streptomyces peucetius CYP147F1 was constructed using three cytochrome P450 structures, CYP107L1, CYPVdh, and CYPeryF, as templates. The lowest energy SPCYP147F1 model was then assessed for stereochemical quality and side-chain environment by Accelrys Discovery Studio 3.1 software. Further activesite optimization of the SPCYP147F1 was performed by molecular dynamics to generate the final SPCYP147F1 model. The substrate limonene was then docked into the model. The model-limonene complex was used to validate the active-site architecture, and functionally important residues within the substrate recognition site were identified by subsequent characterization of the secondary structure. The docking of limonene suggested that SPCYP147F1 would have broad specificity with the ligand based on the two different orientations of limonene within the active site facing to the heme. Limonene with C7 facing the heme with distance of $3.4{\AA}$ from the Fe was predominant.

• Journal of the Korean Society of Safety
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• v.34 no.6
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• pp.29-37
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• 2019

Since construction sites are exposed to outdoor environments, working conditions are significantly dangerous. Thus, wearing of the personal protective equipments such as safety helmet is very important for worker safety. However, construction workers are often wearing-off the helmet as inconvenient and uncomportable. As a result, a small mistake may lead to serious accident. For this, checking of wearing safety helmet is important task to safety managers in field. However, due to the limited time and manpower, the checking can not be executed for every individual worker spread over a large construction site. Therefore, if an automatic checking system is provided, field safety management should be performed more effectively and efficiently. In this study, applicability of deep learning based computer vision technology is investigated for automatic checking of wearing safety helmet in construction sites. Faster R-CNN deep learning algorithm for object detection and classification is employed to develop the automatic checking model. Digital camera images captured in real construction site are used to validate the proposed model. Based on the results, it is concluded that the proposed model may effectively be used for automatic checking of wearing safety helmet in construction site.

• Journal of the Korean Society of Safety
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• v.34 no.5
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• pp.78-86
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• 2019

Various safety measures have been established and implemented in order to reduce construction accidents. However, statistics data on at construction sites still show higher accident and mortality rates than other industries. As a result of investigating the causes of accidents occurring in the construction industry, most accidents resulted from the failure of the system. Therefore, the existing safety management system of the construction site is monitored so that it operates properly in the field, and it is an important factor to actively participate in construction work. In order to overcome this, the government emphasized on-site inspections and planning and implementation confirmation by public institutes to ensure the effectiveness of the safety management system through amendment of the Construction Technology Promotion Act, but the orderer' safety capability and manpower are insufficient at present. Therefore, in this study, the safety management system, such as design for safety, safety management plan, safety check, safety management cost, etc. specified in the Construction Technology Promotion Act, is regularly checked and confirmed to improve the effectiveness of the safety management system as well as to improve the safety management system of the construction site by deriving measures to strengthen the public function of the government.