• Title/Summary/Keyword: engineering school laboratories

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Interaction between Leptospiral Lipopolysaccharide and Toll-like Receptor 2 in Pig Fibroblast Cell Line, and Inhibitory Effect of Antibody against Leptospiral Lipopolysaccharide on Interaction

  • Guo, Yijie;Fukuda, Tomokazu;Nakamura, Shuichi;Bai, Lanlan;Xu, Jun;Kuroda, Kengo;Tomioka, Rintaro;Yoneyama, Hiroshi;Isogai, Emiko
    • Asian-Australasian Journal of Animal Sciences
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    • v.28 no.2
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    • pp.273-279
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    • 2015
  • Leptospiral lipopolysaccharide (L-LPS) has shown potency in activating toll-like receptor 2 (TLR2) in pig fibroblasts (PEFs_NCC1), and causes the expression of proinflammatory cytokines. However, the stimulation by L-LPS was weak eliciting the function of TLR2 sufficiently in pig innate immunity responses during Leptospira infection. In this study, the immune response of pig embryonic fibroblast cell line (PEFs_SV40) was investigated and was found to be the high immune response, thus TLR2 is the predominate receptor of L-LPS in pig cells. Further, we found a strategy using the antibody against L-LPS, to prevent L-LPS interaction with TLR2 in pig cells which could impact on immune activation.

In Situ Observation of Initial Rusting Process of Steel Containing Al Using Synchrotron Radiation X-Rays

  • Morimoto, J.;Yamashita, M.;Uchida, H.;Doi, T.;Kamimura, T.;Miyuki, H.;Konishi, H.;Mizuki, J.
    • Corrosion Science and Technology
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    • v.7 no.2
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    • pp.112-118
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    • 2008
  • We observed initial rusting process of steel containing Al under wet/dry cyclic condition with NaCl solution film using in situ X-ray diffraction spectroscopy at SPring-8 synchrotron radiation facility. It was found that mass fraction of iron oxides such as ${\alpha}-FeOOH$, ${\beta}-FeOOH$ and ${\gamma}-FeOOH$ varied with Al content. Some kinds of Al oxides were also found at the initial stage of corrosion. Those corrosion products might affect the corrosion process and corrosion rate of the steel.

Generation of Coherent Sub-Terahertz Carrier with Phase Stabilization for Wireless Communications

  • Yoshimizu, Yasuyuki;Hisatake, Shintaro;Kuwano, Shigeru;Terada, Jun;Yoshimoto, Naoto;Nagatsuma, Tadao
    • Journal of Communications and Networks
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    • v.15 no.6
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    • pp.569-575
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    • 2013
  • In this paper, we present a photonic approach for generating highly stable coherent sub-terahertz (THz) signals for wireless communications. As proof-of-concept we transmit data at 100 GHz carrier frequency using on-off keying modulation and heterodyne detection. The sub-THz carrier signals are generated by photo-mixing two optical carrier signals at different frequencies, extracted from an optical frequency comb. We introduce a novel system to stabilize the phase of the optical carrier signals. Error-free transmission is successfully achieved up to a bit rate of 8.5 Gbit/s at 100 GHz.

Yield stress measurements in suspensions: an inter-laboratory study

  • Nguyen, Q. Dzuy;Akroyd, Timothy;Kee, Daniel C. De;Zhu, Lixuan
    • Korea-Australia Rheology Journal
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    • v.18 no.1
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    • pp.15-24
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    • 2006
  • The first international inter-laboratory study, involving six laboratories, has been conducted to examine issues associated with yield stress measurements in suspensions. The initial focus of the project was to evaluate the reliability and reproducibility of several common yield stress measuring techniques employed in different laboratories and with different instruments. Aqueous suspensions of colloidal $TiO_2$ at concentrations of 40-70 wt% solids were used as the test fluids. A wide range of instruments and techniques employing both direct and indirect methods were used to determine the yield stress of the samples prepared according to a prescribed procedure. The results obtained indicated that although variations of results existed among different techniques, direct yield stress measurements using static methods produced more reliable and repeatable results than other methods. Variability of the yield stress measured using different techniques within any laboratory however was less significant than variability of the results among different laboratories. The nature and condition of the test suspensions was identified as the most likely factor responsible for the poor reproducibility of yield stress measurements from different laboratories.

A Study on the Methods for Systematic Management of Research Records in Engineering School Laboratories in Korea (우리나라 공과대학 연구실 연구기록의 체계적 관리 방안에 관한 연구)

  • Choi, Hyun-Ok;Rieh, Hae-Young
    • Journal of Korean Society of Archives and Records Management
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    • v.10 no.1
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    • pp.119-139
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    • 2010
  • To be able to present research authenticity, or to cope with patent related cases or any possible law suite for intellectual property, research records management is very important for evidence as well as for providing research information. But record keeping in engineering school laboratory is in an early stage, and lacks associated rules and best practices, which makes systematic research records management hard. This research investigated current practices of engineering school laboratories, analyzed related problems, and provides systematic methods for effective engineering research records management, such as modifying related regulations and process, systematizing the process of research records management, and educating researchers and building infrastructure.

Ergonomic Evaluation of Some University Laboratories

  • Lee, Yun Keun;Lee, Ik Mo;Park, Jeongim;Yoon, Chung Sik;Rhie, Kwang Won;Park, Hee Sok
    • Journal of the Ergonomics Society of Korea
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    • v.32 no.4
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    • pp.397-403
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    • 2013
  • Objective: This article aims to report the results of ergonomic evaluation of some university laboratories. Background: Laboratory researchers are at risk for musculoskeletal injuries during routine laboratory procedures. However, little attention has paid to the working conditions of laboratory researchers, especially in Korea. Method: A checklist and questionnaire was developed and used to analyze the work conditions of 27 laboratories of chemistry, biology and pharmacy. Results: It was found that glove box, clean bench, and computer workstation are better than microscopy, and fume hood tasks, from ergonomic point of view. Conclusion: No domestic guideline is available for ergonomic intervention of laboratories. Further attention and studies are anticipated to improve the working conditions of laboratories.

Lifetime improvement of Organic Light Emitting Diode by Using LiF Thin Film and UV Glue Encapsulation

  • Hsieh, Huai-En;Huang, Bohr-Ran;Juang, Fuh-Shyang;Tsai, Yu-Sheng;Chang, Ming-Hua;Liu, Mark.O.;Su, Jou-yeh
    • 한국정보디스플레이학회:학술대회논문집
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    • 2007.08b
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    • pp.1703-1705
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    • 2007
  • Before the ultra-violet glue encapsulation, the research evaporated LiF thin film on device surface to be the extra packaging layer for improving the lifetime of organic light-emitting diode. The formula of UV glue was specially developed. We found 100 nm LiF is the optimum thickness. The best lifetime obtained by using LiF and special UV glue is 2.4 times longer than those by commercial UV glue.

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Prognostically Significant Fusion Oncogenes in Pakistani Patients with Adult Acute Lymphoblastic Leukemia and their Association with Disease Biology and Outcome

  • Sabir, Noreen;Iqbal, Zafar;Aleem, Aamer;Awan, Tashfeen;Naeem, Tahir;Asad, Sultan;Tahir, Ammara H;Absar, Muhammad;Hasanato, Rana MW;Basit, Sulman;Chishti, Muhammad Azhar;Ul-Haque, Muhammad Faiyaz;Khalid, Ahmad Muktar;Sabar, Muhammad Farooq;Rasool, Mahmood;Karim, Sajjad;Khan, Mahwish;Samreen, Baila;Akram, Afia M;Siddiqi, Muhammad Hassan;Shahzadi, Saba;Shahbaz, Sana;Ali, Agha Shabbir
    • Asian Pacific Journal of Cancer Prevention
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    • v.13 no.7
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    • pp.3349-3355
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    • 2012
  • Background and objectives: Chromosomal abnormalities play an important role in genesis of acute lymphoblastic leukemia (ALL) and have prognostic implications. Five major risk stratifying fusion genes in ALL are BCR-ABL, MLL-AF4, ETV6-RUNX11, E2A-PBX1 and SIL-TAL1. This work aimed to detect common chromosomal translocations and associated fusion oncogenes in adult ALL patients and study their relationship with clinical features and treatment outcome. Methods: We studied fusion oncogenes in 104 adult ALL patients using RT-PCR and interphase-FISH at diagnosis and their association with clinical characteristics and treatment outcome. Results: Five most common fusion genes i.e. BCR-ABL (t 9; 22), TCF3-PBX1 (t 1; 19), ETV6-RUNX1 (t 12; 21), MLL-AF4 (t 4; 11) and SIL-TAL1 (Del 1p32) were found in 82/104 (79%) patients. TCF3-PBX1 fusion gene was associated with lymphadenopathy, SIL-TAL1 positive patients had frequent organomegaly and usually presented with a platelets count of less than $50{\times}10^9/l$. Survival of patients with fusion gene ETV6-RUNX1 was better when compared to patients harboring other genes. MLL-AF4 and BCR-ABL positivity characterized a subset of adult ALL patients with aggressive clinical behaviour and a poor outcome. Conclusions: This is the first study from Pakistan which investigated the frequency of5 fusion oncogenes in adult ALL patients, and their association with clinical features, treatment response and outcome. Frequencies of some of the oncogenes were different from those reported elsewhere and they appear to be associated with distinct clinical characteristics and treatment outcome. This information will help in the prognostic stratification and risk adapted management of adult ALL patients.