• Journal of Ginseng Research
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• v.16 no.2
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• pp.129-137
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• 1992

This study was carried out to investigate the localization of lipids and lipase activity with lipid staining and cytochemical technique in endosperm cells of Panax ginseng C.A. Meyer seed. In endosperm cells of indehiscent seed, protein bodies facing the umbiliform layer are different in electron density during the various degraded processes. Gradually, protein matrix near the cell wall was lysed and electron lucent inclusions appeared on umbiliform layer. The protein body with high electron density and the spherosome with low electron density were observed in endosperm cells. As a result of lipid staining, electron density of spherosome is more intense than those of the protein matrix within the protein body in endosperm cells of indehiscent seed. Free spherical spherosomes within the umbiliform layer have a high electron density. The spherical spherosomes were more electron densed and were uniform in comparison with the cytoplasmic proteinaceous granules in endosperm cells of seed with red seed coat. The major component of spherosome was determined to be lipid. Lipase activity occurs in the spherosome and near the endosperm cell wall facing the umbiliform layer. Cytochemical reaction products of lipase were observed in the spherosome membrane and in the inner regions of spherosome. After protein bodies were digested, lipase activities were observed in free spherosomes and near the cell wall of endosperm cells. Umbiliform layer composing of fibrillized wall and digested materials of the endosperm cell showed a little lipase reaction products.

• Journal of Ginseng Research
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• v.16 no.1
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• pp.37-43
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• 1992

Structural changes of the endosperm of Panax ginseng C.A. Meyer from fertilization to germination were investigated by light microscope. The endosperm of the ginseng seed is cellular type. Since endosperm cells adjacent embryo continuously breakdown and disappear with the elongation of embryo, the real of endosperm is gradually decreased. As the anatropous ovules of immature seed with green seed coat developes more and more, ovary cells adjacent ovary cavity become abundant by the periclinal division, their size is decreased, hypotrophy of cell wall discern, and they are gradually differentiated in seed coat. Though embryo responds strongly to basic dye at the stage of completion of endosperm formation, tissue of endosperm responds to acidic dye positively Cell wall of embryo and endosperm are composed of primary cell wall not lignified. Endosperm cells adjacent embryo begin to breakdown in the endosperm tissue of indehiscent seed before the beginning of the after-ripening. Dehiscent seed of which seed coat is opened through after-ripening represent the form as a seedling in the result of embryo developments with the formation of organs; radicle, cotyledon, plumule. Umbilifom layer represents strong positive response to the toluidine blue and the basic function. Umbiliform layer that endosperm cells breakdown and disappear is observed clearly at the periphery of the embryo cotylemon, while slightly at the periphery of the radicle.

• Applied Microscopy
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• v.14 no.2
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• pp.15-28
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• 1984

The endosperm cells and the umbiliform layer of ginseng (Panax ginseng C.A. Meyer) seed are studied with light and electron microscope. Differentiated mitochondria, ER cisternae, proplastids and ribosomes are characteristically observed in the endosperm cells of matured seed. The cell inclusions contain the protein bodies and the spherosomes. Protein body contains, in proteinaceous matrix, globoids and crystalloids. Particularly the crystalloids have the lattice structure, and the formation of globoids is closely related with ER. Umbiliform layer has the positive reaction on alcian blue (pH 2.5) and the metachromasis on the toluidine blue. The umbiliform layer is formed by autolysis of endosperm cells, and composed of the deformated cell wall and the lipoprotein bodies. Particularly a part of the lipoprotein body and the fibrilar network structure have the positive reaction on acid phosphatase.

• Applied Microscopy
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• v.24 no.3
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• pp.67-77
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• 1994

Storage material of endosperm cells digested by various enzymes should be transported to the embryo. At this time, the cellulose of the endosperm cell wall is guessed to be hydrolyzed by the cellulase enabling to transfer the storage material from the endosperm cells to the embryo. Therefore, this study has been carried out to investigate the ultrastructure of endosperm and the localization of the cellase activity on Cannabis sativa L. during germination. Endosperm cells contain a large number of lipid bodies and protein bodies with globoids as the storage material. During gemination they are gradually degenerated, however, the former almost remain until the cells are completely digested. Electron-microscopical reaction products of cellulase on endosperm cells are present. The closer the embryo, the more amount of reaction products on the endosperm cell wall are appeared.

• Journal of Plant Biology
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• v.35 no.1
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• pp.45-51
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• 1992

The changes of protein bodies in endosperm cells of both seeds with red seed coat and indehiscent seeds of Panax ginseng C.A. Meyer have been investigated in relation to the embryo development. In the early stage of seeds with red seed coat, spherical spherosomes were distributed in endosperm cells. Protein bodies were formed from vacuoles containing the storage protein. Cell organelles were hardly observed in the cytoplasm. In the late stage of the seed with red seed coat, the endosperm was filled with spherosomes and protein bodies. The protein bodies consisted of amorphous inclusions with high electron density or proteinaceous matrix with even electron density. In the seed of in dehiscence, the protein body in endosperm cells contained globoids and protein crystalloids. The globoid of protein body had a electron dense materials. Umbiliform layer was formed between embryo and endosperm. The deformation patterns of endosperm cell wall and the cellulose microfibril were observed in endosperm cells near the umbiliform layer. Umbiliform layer consisted of lipid body and autolyzed cell debris. The protein body of endosperm cell near the umbiliform layer showed various degenerative patterns, and so electron density of proteinaceous matrix was gradually decreased.reased.

• Journal of Plant Biology
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• v.36 no.4
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• pp.327-335
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• 1993

The active sites, intracellular transport, function of cellulase in association with the disintegration of the storage materials of the endosperm cells during seed maturation and after-ripening of Panax ginseng C.A. Meyer seeds were studied by electron microscopy. Cytochemical activities of the cellulase occurred in protein bodies and vesicles of endosperm cells in seed with red seed coat. In after-ripening seed, the activities were strongly found in the cell wall of endosperm near the umbiliform layer and on neighbouring vesicles, so it is assumed that these cells begin to be decomposed. Cellulase activities were initiated before the decomposition of storage materials. But, no activity was observed in the umbiliform layer, so it is suggested that cellulase lose its activity after the completion of lysis process.

• Journal of Plant Biology
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• v.35 no.1
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• pp.53-60
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• 1992

This study has been carried out to investigate the ultrastructural changes in the associated with the disintegration of the storage materials in endosperm cell of ginseng (Panax ginseng C.A. Meyer) seed during after-ripening with light and electron microscope. The protein body of endosperm cells near the umbiliform layer showed various degenerative patterns, and so electron density of proteinaceous matrix was gradualJy decreased during afterripening. These results indicate that the decomposition of endosperm was already initiated during after-ripening. As the degeneration of endosperm was more progressed after the dehiscence of seed, non-decomposed part of protein body appeared amorphously with high electron density. Decomposed protein bodies were vacuolized with the loss of their matrix and gradually expanded by fusion. Also, spherosomes were gradually dissolved with the lowered electron density during the degeneration of endosperm. The vesicles of dictyosomes near the cell wall are observed in endosperm contacting with umbiliform layer and are fused with plasma membrane. Umbiliform layer which was the complex of the decomposed remnants of lysis and materials has strong stainability for toluidine blue and basic fuchsin.

• Journal of Plant Biology
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• v.34 no.3
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• pp.201-213
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• 1991

This study has been carried out to investigate the ultrastructural changes, formation of storage materials in endosperm cells with electron microscope during the seed formation of Panax ginseng C.A. Meyer. In the early stage of seed formation with green seed coat, the endosperm was cellular type. Cell plate was largely composed of dictyosome vesicles in early stage of wall formation after mitosis. Central vacuole was gradually subdivided into several small-sized vacuoles. During the differentiation of plastids, some proplastid was replaced by amyloplast with starch grains and lamellar structure. A number of mitochondria with well developed cristae were distributed in cytoplasm. Rough endoplasmc reticulum, dictyosome, microbody, free ribosomes and polysomes were evenly distributed in cytoplasm. Spherical spherosomes were formed from dictyosome containing the lipid materials of even electron density. Protein bodies were formed by interfusing between vacuoles and vesicles derived from rough endoplasmic reticulum which contained the amorphous protein of high electron density.

• Korean Journal of Food Science and Technology
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• v.28 no.4
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• pp.720-729
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• 1996

Effect of the cellulase treatment on the physicochemical properties of three varieties of rice (chucheongbyeo, chosengtongilbyeo and IR 36) and the texture of cooked rice were investigated. The swelling power and solubility of the rice flours were increased and amylographic viscosities, especially cold viscosities were decreased by cellulase treatment in all varieties. Gel chromatography of soluble carbohydrates from cellulase-treated rice flours on sepharose 2B-CL showed a singificant increase of low molecular weight $(10^{4})$ fraction which might be produced upon hydrolysis in endosperm cell wall constituents. The hardness of cooked rices prepared from cellulose-treated rices significantly decreased.

• Applied Biological Chemistry
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• v.39 no.5
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• pp.379-383
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• 1996

The ultrastructure of the compound starch granules and the protein bodies of Odaebyeo rice of early matured variety were examined by light microscope and electron microscope. The endosperm cell appealed rectangular or octangular shape on the cross section. The thickness of cell wall containing of membraneous materials was about $0.5\;{\mu}m$ in diameter. The starch cell was filled compactly with globular or oval shaped compound starch granules with the size of $20{\sim}25\;{\mu}m$ in diameter. The compound starch granules were consisted of central core starch granule and concentrical $2{\sim}3$ layers of starch granules. The average thickness of the starch granules were about $5\;{\mu}m$. Most protein bodies were found in the aleurone layer The globular protin bodies were scattered near the compound starch granules and $2.5{\sim}3\;{\mu}m$ in diameter. The protein bodies composed of central electron dense materials and peripheral electron loose materials in limiting membrane.