• Title/Summary/Keyword: endophytic

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Identification of Endophytic Bacteria Isolated from Rusty-colored Root of Korean Ginseng (Panax ginseng) and Its Induction (적변삼으로부터 분리한 내생세균의 동정 및 적변 유발)

  • Choi, Jae-Eul;Ryuk, Jin-Ah;Kim, Jin-Hee;Choi, Chun-Hwan;Chun, Jong-Sik;Kim, Young-Jun;Lee, Hyang-Burm
    • Korean Journal of Medicinal Crop Science
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    • v.13 no.1
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    • pp.1-5
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    • 2005
  • While the rusty-colored root is common in ginsengs culture and, often results in a severe economic loss, the major factors have not been found. This study was focused on the determination of a potential relationship between rusty root and endophytic bacteria. The number of endophytes was $9.6\;{\times}\;10^1{\sim}1.5\;{\times}\;10^2\;cfu/g$ fw in normal ginseng roots compared to $3.7\;{\times}\;10^6{\sim}5.1\;{\times}\;10^7\;cfu/g$ fw in rusty ones. Of 31 isolates from rusty ginseng roots, twenty-four isolates repeatedly induced severe to moderate rust on root while seven isolates induced slight rust. The bacteria responsible for rusty ginseng roots were mainly Gram negative aerobic. Rust inducing bacteria were identified as Agrobacterium tumefaciens, A. rhizogenes, Burkholderia phenazinium, Ensifer adharens, Lysobacter gummosus, Microbacterium luteolum, M. oxydans, Pseudomonas marginalis, P. veronii, Pseudomonas sp., Rhizobium leguminosarum, R. tropica, Rhodococcus erythropolis, Rh. globerulus, Variovorax paradoxus on the basis of bacteriological characters and 16S rDNA sequences analysis. The results in this study strongly suggested that the rusty ginseng roots were produced by infection and growth of endophytic bacteria.

Diversity and Plant Growth Promoting Capacity of Endophytic Fungi Associated with Halophytic Plants from the West Coast of Korea

  • Khalmuratova, Irina;Kim, Hyun;Nam, Yoon-Jong;Oh, Yoosun;Jeong, Min-Ji;Choi, Hye-Rim;You, Young-Hyun;Choo, Yeon-Sik;Lee, In-Jung;Shin, Jae-Ho;Yoon, Hyeokjun;Kim, Jong-Guk
    • Mycobiology
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    • v.43 no.4
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    • pp.373-383
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    • 2015
  • Five halophytic plant species, Suaeda maritima, Limonium tetragonum, Suaeda australis, Phragmites australis, and Suaeda glauca Bunge, which are native to the Muan salt marsh of South Korea, were examined for fungal endophytes by sequencing the internal transcribed spacer (ITS) region containing ITS1, 5.8S rRNA, and ITS2. In total, 160 endophytic fungal strains were isolated and identified from the roots of the 5 plant species. Taxonomically, all 160 strains belonged to the phyla Ascomycota, Basidiomycota, and Zygomycota. The most dominant genus was Fusarium, followed by the genera Penicillium and Alternaria. Subsequently, using 5 statistical methods, the diversity indices of the endophytes were determined at genus level. Among these halophytic plants, P. australis was found to host the greatest diversity of endophytic fungi. Culture filtrates of endophytic fungi were treated to Waito-C rice seedlings for plant growth-promoting effects. The fungal strain Su-3-4-3 isolated from S. glauca Bunge provide the maximum plant length (20.1 cm) in comparison with wild-type Gibberella fujikuroi (19.6 cm). Consequently, chromatographic analysis of the culture filtrate of Su-3-4-3 showed the presence of physiologically active gibberellins, $GA_1$ (0.465 ng/mL), $GA_3$ (1.808 ng/mL) along with other physiologically inactive $GA_9$ (0.054 ng/mL) and $GA_{24}$ (0.044 ng/mL). The fungal isolate Su-3-4-3 was identified as Talaromyces pinophilus.

Entomopathogenic fungal infection of Allomyrina dichotoma in mass breeding conditions

  • Kwak, Kyu-Won;Nam, Sung-Hee;Choi, Hyo-Won;Choi, Ji-Young;Lee, Seok-Hyun;Kim, Hong-Geun;Han, Myung-Sae;Park, Kwan-Ho
    • International Journal of Industrial Entomology and Biomaterials
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    • v.31 no.1
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    • pp.20-24
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    • 2015
  • Increased use of rhinoceros beetles (Allomyrina dichotoma ) for food and medicine, and their elevated value as pets has led to an increase in the number of their breeding farms. Mass breeding of the insects in these farms leads to entomological diseases. In this study, we investigate cannibalism resulting from overcrowded breeding of A. dichotoma larvae, as well as secondary fungal infections in epidermal wounds in the surviving larvae. Some of the fungi detected in the present study showed entomopathogenicity, and the larvae showed different times of death. In particular, larvae infected with Clonostachys sp., an endophytic plant fungus, grew for a long time after infection, but died without pupating.