• Korean Journal of Ecology and Environment
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• v.36 no.2 s.103
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• pp.97-107
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• 2003

Fish vitellogenin produces in female liver during oogensesis through estradiol cycle, and produces even in male liver by endocrine-disrupting chemicals (EDCs) such as alkylphenols. The resulting effects of EDCs lead to the low fecundity of female and the feminization (eg. shrinkage of testis) in male. Especially, the production of vitellogenin in male indicates the environmental contamination of EDCs, resulting in the modulation of gene expression profiles and the monitoring of environmental contamination at specific area. In this paper, we suggest that fish vitellogenin is useful for biomonitoring for environmental contamination and would be substantially useful as a biomarker for a detection of EDCs in aquatic environment.

• Women's Health Nursing
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• v.25 no.4
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• pp.423-433
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• 2019

Purpose: The study aimed to examine the effects of sleep pattern, stress, and attitude towards menstruation, and behavior that causes a reduction in exposure to endocrine disrupting chemicals (EDCs) in the course of premenstrual syndrome (PMS) in female adolescents. Method: In total, 200 girls attending middle school in K city were recruited via convenience sampling between December 11 and 31, 2018, and 192 subjects were selected for the analysis. Data were analyzed using descriptive statistics, an independent t-test, ANOVA, Pearson's correlation coefficient, and multiple regression. Results: Overall, 9 (4.7%), 152 (79.2%), and 31 (16.1%) students had morning-, intermediate-, and evening-type sleep pattern, respectively. The mean scores for school-related stress were highest. Scores for stress regarding relationships with friends were the lowest. The mean score for attitudes toward menstruation was 72.33 out of 147. The mean score for behavior that led to a reduction in EDCs exposure was 56.57 out of 85. The mean scores for PMS severity were 25.30 and 38.39 out of 60. Analysis of factors affecting PMS revealed that severity of menstrual pain (β=.28), use of analgesics during menstruation (β=.17), family history of PMS (β=-.16), stress (β=.19), and behavior that reduces EDCs exposure (β=-.17) exerted significant effects on PMS. The explanatory power of these variables was 37%. Conclusion: The findings propose the necessity of a health intervention program including educational information, which can reduce PMS in adolescents, induce the behaviors which can decrease exposure to EDCs, and activities for managing the stress levels.

• Journal of Radiation Industry
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• v.8 no.2
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• pp.71-76
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• 2014

Endocrine disrupting chemicals (EDCs) and potential EDCs are mostly man-made, found in various materials such as pesticides, additives or contaminants in food, and personal care products. The high energy ionizing radiation has the ability to remove the EDCs with a very high degree of reliability and in a clean and efficient manner. The ionizing radiation interacts with EDCs both directly and indirectly. Direct interaction takes place with EDCs and the structure of EDCs is destroyed or changed. During indirect interaction, radiolysis products of water result in the formation of highly reactive intermediates which then react with the target molecules, culminating in structural changes. To confirm the radiation reduction of EDCs in industrial sludge, a pilot scale experiment up to 50 kGy of electron beam (EB) was conducted with samples from the textile dyeing industries. The experimental result showed the over 90% of reduction of Nonylphenol (NP) and Di(2-ethylhexyl) phthalate (DEHP) at around 10 kGy of absorbed doses.

• YAKHAK HOEJI
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• v.44 no.5
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• pp.416-423
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• 2000

There is a growing concern that a wide variety of chemicals released into the environment can disrupt the endocrine system of fish, wildlife and humans. Endocrine disrupting chemicals (EDCs) include pesticides such as DDT lindane and atrazine, the food packaging chemicals, phthalates and bisphenol A, alkylphenol ethoxylate detergents and the chemical industry by-products, dioxins. Xenoestrogens in the environment have been argued about health risk, because of estrogen mimetic chemicals are exposed only small amounts to human. A number of in vivo and in vitro assays are now in use to assess the activity of xenoestrogens in the environment. A human breast cancer cell line (MCF-7) was used to develop in vitro screening assay for the detection of xenoestrogenic environmental pollutants. The E-SCREEN (MCF7-BUS) assay is proposed as a reliable, easy and rapid-to-perform method. To optimize and validate this method before it can be used routinely, several phenol compounds and pesticides suspected to be estrogenic were tested using I-SCREEN assay. The results showed that this method is a valuable tool for screening potential estrogen-mimicking environmental pollutants and quantitative determination of estrogeniciy.

• Korean Chemical Engineering Research
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• v.47 no.6
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• pp.775-780
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• 2009

The efficacy of nitrifying sludge existed in biological nutrient removal process was examined for possible removal of endocrine disrupting chemical(EDC) in the effluent of wastewater treatment plant. Some of ammonia oxidizing bacteria causes ammonia oxidation mediated by ammonia monooxygenase(AMO) activity, which has low substrate specificity resulting in cometablic degradation of several chemicals. In this study, the removal of three model EDCs such as bisphenol A(BPA), nonylphenol(NP) and dibutyl phthalate(DBP) was studied in batch cultures using nitrifying sludge, BOD-oxidizing sludge with low nitrifying activity, and sterilized sludge. Nitrifying sludge showed higher initial removal rates in all batches of three EDCs when it was fed with ammonium as an energy source. The acclimation time was required for the removal of EDCs in batches using BOD-oxidizing sludge or nitritefed nitrifying sludge. That retardation seemed to attribute to the slow growth of cells using the EDCs while ammonium-fed nitrifying sludge could degrade EDCs through simultaneous cooxidation with ammonia oxidation. Sterilized sludge was also tested under the same conditions in order to find the contribution of physical adsorption to the removal of EDCs. About 10~20% of initial EDCs dose was removed when using sterilized sludge. Thus the biological activity is likely to play major role for the degradation of BPA, NP, and DBP rather than the physical adsorption from wastewater.

• Archives of Pharmacal Research
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• v.26 no.9
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• pp.697-705
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• 2003

The alkylphenols, chlorophenols, and bisphenol A were determined by gas chromatography/mass spectrometry-selected ion monitoring (GC/MS-SIM) followed by two work-up methods for comparison: isobutoxycarbonyl (isoBOC) derivatization and tert-butyldimethylsilyl (TBDMS) derivatization. Eleven endocrine disrupting chemicals (EDCs) of phenols in biological samples were extracted with acetonitrile and then the acetonitrile layer underwent freezing filtration 6$0^{\circ}C$ for 2 hours. Solid-phase extraction (SPE) was used with XAD-4 and subsequent conversion to isoBOC or TBDMS derivatives for sensitivity analysis with the GC/MS-SIM mode. For isoBOC derivatization and TBDMS derivatization the recoveries were 92.3∼150.6% and 93.8∼108.3%, the method detection limits (MDLs) of bisphenol A for SIM were 0.062 $\mu$ g/kg and 0.010 $\mu$ g/kg, and the SIM responses were linear with the correlation coefficient varying by 0.9755∼0.9981 and 0.9908∼0.9996, respectively. When these methods were applied to mackerel samples, the concentrations of the 11 phenol EDCs were below the MDL.

• Korean Journal of Environmental Biology
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• v.29 no.1
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• pp.74-80
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• 2011

In this study we present a mammalian cell culture model that allows to study the effect of endocrine disruptors (EDCs) on aromatase activity of aquatic amphibian, Bombina orientalis. Bombina orientalis aromatase gene was subcloned into a mammalian expression vector and subsequently transfected to mammalian cells. Although the protein expression level of Bombina orientalis aromatase was low, it had a significant aromatase activity. When EDCs were added to aromatase transfected cells, aromatase activity was significantly decreased. We report here that this system may be used to monitor the effect of EDCs on aromatase activity of aquatic organisms.

• Proceedings of the Korea Society of Environmental Toocicology Conference
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• 2003.05a
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• pp.145-145
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• 2003

A method for the determination of alkylphenols, chlorophenols and bisphenol A in paper materials using GC/MS-SIM has been developed. Eleven endocrine disrupting chemicals (EDCs) of phenols in paper samples were extracted with acetonitrile. Also, solid-phase extraction (SPE) with XAD-4 and subsequent conversion to isobutoxycarbonyl derivatives or tert.-butyldimethylsilyl derivatives for sensitive analysis with the selected ion-monitoring (SIM) mode. The recoveries were 82.4∼108.8 % by area ratio of pheranthrene-d$\sub$10/ vs bisphenol A d$\sub$l6/. (isoBOC derivatization and TBDMS derivatization) The SIM responses were linear with the correlation coefficient varying 0.9717∼0.9995 (isoBOC derivatization), and 0.9842∼0.9980 (TBDMS derivatization). The range of concentrations was respectively, 0.95∼l.44 ng/g in 2,4-dichlorophenol, 1.01∼1.17 ng/g in t-butylphenol, 2.17∼5.84 ng/g in pentachlorophenol, 12.68∼14.88 ng/g in nonylphenol and 30.84∼153.72 ng/g in bisphenol A.

• Toxicological Research
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• v.16 no.1
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• pp.33-37
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• 2000

Hershberger assay is known as one of the in vivo-short-term scrrning assays for endocrine disrupting chemicals (EDCs), but this method is not a validated test system. In the present study, the establishment of Hershberger assay to detect EDCs was tried using a model substance, di(n-butyl)phthalate (DBP), a plasticizer for plastics. Thirty-six immature male rats were randomly assigned to six groups: DBP 0, 40, 200, and 1000mg/kg, a positive control (flutamide 20 mg/kg), and a combination group(DBP 1000mg/kg and testosterone 50 ug/kg). DBP and flutamide were administered by gavage to male rats from day 21 to 40 post partum. Testosterone was subcutaneously injected during the same period. We evaluated body weigth gain, weights of ventral prostate, seminal vesicle, and levator ani and bulvocavernous muscle, and serum concentrations of testosterone and lutenizing hormone in male rats. The weights of seminal vesicle and levator ani and bulvocavernous muscle of males receiving 1000mg/kg of DBP was significantly lower than controls. There was no effect of DBP-treatment on body weight gain, prostate weight, and hormone concentrations. In the positive control group, the weights of seminal vesicle and levator ani and bulvocavernous muscle of males receiving 20mg/kg of flutamide were significantly lower than controls. In the combination group, there was no effect of co-treatment of DBP and testosterone on all parameters effect against DBP. This method was found to be a useful short-term screening assay system for EDCs.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.28 no.4
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• pp.406-415
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• 2018

Objective: The purpose of this study was to investigate the hazards of cosmetic ingredients in Korea. Methods: An Excel database of cosmetic ingredients was developed on a website(Korea Cosmetic Ingredient Dictionary) and used for a hazard assessment based on Tox-free, a database containing toxic information such as on carcinogens, mutagens or reproductive toxicants(CMRs) and endocrine disrupting chemicals(EDCs), as well as other regulated chemicals in Korea. Results: A total of 16,605 chemicals were registered with the cosmetic ingredient dictionary and 308 of them were identified as either CMRs or EDCs. CMRs included formaldehyde and nickel gluconate, and EDCs included parabens, benzophenon, styrene, and toluene. Reproductive toxicants such as xylene, zinc chloride, toluene, and formaldehyde were regulated by the Occupational Safety and Health Act and the Act on the Registration and Evaluation, etc. of Chemical Substances(the Chemical Control Act). Conclusions: Regulations on cosmetics components should be strengthened, and the right to know about cosmetics containing hazardous chemicals should be guaranteed.