• Title/Summary/Keyword: encapsulation media

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Development of Artificial Seeds by Encapsulation Zygotic Embryo of Apple (Malus pumila var. domestica) (사과의 접합자배 encapsulation에 의한 인공종자 개발)

  • Choi, Pil Son
    • Korean Journal of Plant Resources
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    • v.34 no.1
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    • pp.59-63
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    • 2021
  • To study for germination rate of apple artificial seeds encapsulated with 2% alginic acids, the rates of germination for artificial seeds of various embryos with partial elimination of cotyledon measured by adding 80 mg/L gibberellic acid or/and nutritional media. The germination rate of artificial seeds produced embryo with 3/4 cotyledon (3/4 CE), embryo with 1/2 cotyledon (1/2 CE), embryo with 1/4 cotyledon (1/4 CE), and embryo without cotyledon (NCE) were gradually decreased with compare to normal embryo, but it's considerably recovered on the alginic acid containing MS solution or/and 80 mg/L GA3. In special, the low frequency (55.81%) of germination for artificial seeds of 1/2 cotyledon embryo with alginic acid was increased by treatment with combination of alginic acid, 3% sucrose, and 80 mg/L gibberellic acid (98.74%). In case of embryo without cotyledon, the low frequency of embryo without cotyledon (24.44%) in alginic acid almost recovered to 82.45% in alginic acid with MS solution and 80 mg/L gibberellic acid. The results shown that the germination of embryo was decreased by the removal of cotyledon, and demonstrated that the low rate of germination could be considerably increased by adding GA3 (80 mg/L) or/and nutrition.

Design of 8K Broadcasting System based on MMT over Heterogeneous Networks

  • Sohn, Yejin;Cho, Minju;Paik, Jongho
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.11 no.8
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    • pp.4077-4091
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    • 2017
  • This paper presents the design of a broadcasting scenario and system for an 8K-resolution content. Due to an 8K content is four times larger than the 4K content in terms of size, many technologies such as content acquisition, video coding, and transmission are required to deal with it. Therefore, high-quality video and audio for 8K (ultra-high definition television) service is not possible to be transmitted only using the current terrestrial broadcasting system. The proposed broadcasting system divides the 8K content into four 4K contents by area, and each area is hierarchically encoded by Scalable High-efficiency Video Coding (SHVC) into three layers: L0, L1, and L2. Every part of the 8K video content divided into areas and hierarchy is independently treated. These parts are transmitted over heterogeneous networks such as digital broadcasting and broadband networks after going through several processes of generating signal messages, encapsulation, and packetization based on MPEG media transport. We propose three methods of generating streams at the sending entity to merge the divided streams into the original content at the receiving entity. First, we design the composition information, which defines the presentation structure for displays. Second, a descriptor for content synchronization is included in the signal message. Finally, we define the rules for generating "packet_id" among the packet header fields and design the transmission scheduler to acquire the divided streams quickly. We implement the 8K broadcasting system by adapting the proposed methods and show that the 8K-resolution contents are stably received and serviced with a low delay.

Optimization of Culture Conditions and Encapsulation of Lactobacillus fermentum YL-3 for Probiotics (가금류 생균제 개발을 위한 Lactobacillus fermentum YL-3의 배양조건 최적화 및 캡슐화)

  • Kim, Kyong;Jang, Keum-Il;Kim, Chung-Ho;Kim, Kwang-Yup
    • Korean Journal of Food Science and Technology
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    • v.34 no.2
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    • pp.255-262
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    • 2002
  • This experiment was performed to improve the stability of Lactobacillus fermentum YL-3 as a poultry probiotics. The culture conditions that improve acid tolerance of L. fermentum YL-3 were investigated by changing several factors such as medium composition, temperature, anaerobic incubation and culture time. Also, L. fermentum YL-3 was encapsulated with alginate, calcium chloride and chitosan. The stable culture conditions of L. fermentum YL-3 were obtained in anaerobic incubation using MRS media without tween 80 for 20 hour at $42^{\circ}C$. The capsule after treatment with 1% chitosan was formed close membrane by a bridge bond. Immobilization of L. fermentum YL-3 in capsule was observed by confocal laser scanning microscopy, and cell viability was $2.0{\times}10^9\;CFU/g$ above the average. L. fermentum YL-3 capsule after acid treated at pH 2.0 for 3 hour survived about 40%, but those encapsulated with 1% chitosan survived about 65%. Survival rate of capsule stored at room temperature decreased about $2{\sim}3$ log cycle during 3 weeks, but viability of capsule stored at $4^{\circ}C$ during 3 weeks maintained almost $10^8\;CFU/g$ levels.