• Biotechnology and Bioprocess Engineering:BBE
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• 제11권1호
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• pp.84-87
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• 2006

Batch binding experiments were performed to assess the recovery performance of glyceraldehyde 3-phosphate dehydrogenase (G3PDH) bound to the unshielded and polymer (polyvinyl pyrrolidone. PVP)-shielded dye-ligand (Cibacron Blue 3GA) adsorbent. The adoption of a polymer-shielded, dye-ligand technique facilitated the elution efficiency of bound G3PDH. It was demonstrated that the recovery of G3PDH using polymer-shielded dye-ligand adsorption yielded higher elution efficiency, at 60.5% and a specific activity of 42.3 IU/mg, after a low ionic strength elution (0.15 M NaCl). The unshielded dye-ligand yielded lower elution efficiency. at 6.5% and a specific activity of 10.2 IU/mg.

• 공업화학
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• 제9권4호
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• pp.574-578
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• 1998

전기화학적 이온교환전극을 양이온 교환수지 Amberlite IRP-64와 결합제로서 Stylen-Buthylene-Rubber (SBR)를 혼합하여 압축성형 방법으로 제작하여, 전극전위와 전해질의 pH에 따른 구리이온의 흡착과 용출특성을 고찰하였다. 흡착공정에서는 -1800 mV에서 최대 흡착속도를 나타내었으며 90분 경과 후,92%의 흡착율을 나타내었다. 용출공정의 경우, 본 실험영역에서는 전위의 증가에 비례하여 용출율은 증가되어 나타났으며, 3600 mV에서 50분 이후 88%의 용출율을 나타내었다. 전기화학적 이온교환 전극 근처의 국부적인 pH 변화는 흡착과 용출공정에 크게 영향을 미치는 것으로 사료되며, 전해질의 pH가 각각 염기성과 산성에서 높은 흡착율과 용출율을 나타내었다.

• 한국염색가공학회지
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• 제21권4호
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• pp.1-10
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• 2009

This paper surveys the physical properties of sheath/core nylon/PET high hollow composites filaments and its fabrics according to the various elution conditions such as concentration of elution, eluted time and eluted temperature. For this purpose, sheath/core nylon/PET filament was texturized and four kinds of fabric specimens were woven with different warp and weft densities. These grey fabrics were eluted with two kinds of concentrations of NaOH (30g/l, 40g/l), three kinds of eluted temperatures $50^{\circ}C,\;60^{\circ}C,\;85^{\circ}C$) and two kinds of eluted times (60min, l20min). The elution characteristics of these specimens were investigated and discussed with different elution conditions. In addition, the mechanical properties such as extensibility, bending rigidity, shear modulus and compressional work of these specimens aceording to the elution conditions were analysed and summarized with cross-sectional shapes of eluted filaments measured by SEM.

• 한국응용과학기술학회지
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• 제27권4호
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• pp.475-481
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• 2010

Elution effect was studied by the reactions between Electric arc furnace(EAF)dust and conc. or dil. sulfuric acid for the purpose of recoverying of the useful metals afterwards. The methods of mixing with acids and EAF dust for the elutions were depend on the ratios of acid/EAF dust. Experimental results showed that increased the ratios of sulfuric acids, decreased the elution effects are on the Zn, Fe and Mn, and conc. sulfuric acid showed higher elution effects than dil. sulfuric acids for the all metals. But in case of Pb, dil. sulfuric acid showed higher elution effects than conc. sulfuric acids conversely. Furthermore, the reason of the extreamly low quantities of eluted Pb is caused by the low solubilities by sulfuric acids compared to the other metals, as the results of the insoluble Pb is removed by the process of filtering.

• 공업화학
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• 제10권5호
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• pp.672-676
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• 1999

흡착을 이용한 분리공정에서 가장 기본적인 것은 흡착평형식이다. 본 연구에서는 용출곡선법과 frontal analysis(FA)을 사용하여 용출곡선에서 직접 흡착평형식을 구하였다. 역상 액체 크로마토그래피(RP-HPLC)에서 시료는 5'-GMP이고 buffer로서 sodium phosphate를 물에 첨가하여 이동상으로 사용하였다. 이 실험조건에서는 시료의 양이 증가함에 따라서 체류시간이 감소하고 피크의 앞부분이 매우 경사가 심한 Langmuir 흡착평형식이 되었다. 용출곡선을 이용한 방법을 이용하여 Langmuir 흡착평형식의 매개변수를 최적화하여 구하였고 FA 방법을 이용하여 고정상의 농도를 용출곡선으로부터 측정하고 회귀분석에 의하여 흡착평형식을 측정하였다. FA 방법에 비해서 용출곡선법은 시료의 양이 적게 소모되고 실험 횟수도 1-2번 정도로 간편하였다. 이동상에 포함된 sodium phosphate의 농도에 따라서 Langmuir 흡착평형식의 매개변수에 미치는 영향을 고찰하였다.

• 대한치과교정학회지
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• 제42권6호
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• pp.297-306
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• 2012

Objective: This in vitro study was undertaken to evaluate the physical, chemical, and biological properties of commercially available metal orthodontic brackets in South Korea, because national standards for these products are lacking. Methods: Four bracket brands were tested for dimensional accuracy, (manufacturing errors in angulation and torque), cytotoxicity, composition, elution, and corrosion: Archist (Daeseung Medical), Victory (3M Unitek), Kosaka (Tomy), and Confidence (Shinye Odontology Materials). Results: The tested rackets showed no significant differences in manufacturing errors in angulation, but Confidence brackets showed a significant difference in manufacturing errors in torque. None of the brackets were cytotoxic to mouse fibroblasts. The metal ion components did not show a regular increasing or decreasing trend of elution over time, but the volume of the total eluted metal ions increased: Archist brackets had the maximal Cr elution and Confidence brackets appeared to have the largest volume of total eluted metal ions because of excessive Ni elution. Confidence brackets showed the lowest corrosion resistance during potentiodynamic polarization. Conclusions: The results of this study could potentially be applied in establishing national standards for metal orthodontic brackets and in evaluating commercially available products.

• Bulletin of the Korean Chemical Society
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• 제22권5호
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• pp.503-506
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• 2001

Separation of lithium isotopes was investigated by chemical ion exchange with a hydrous manganese(IV) oxide ion exchanger using an elution chromatography. The capacity of manganese(IV) oxide ion exchanger was 0.5 meq/g. One molar CH3COO Na solution was used as an eluent. The heavier isotope of lithium was enriched in the solution phase, while the lighter isotope was enriched in the ion exchanger phase. The separation factor was calculated according to the method of Glueckauf from the elution curve and isotopic assays. The single stage separation factor of lithium isotope pair fractionation was 1.021.

• Biotechnology and Bioprocess Engineering:BBE
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• 제6권1호
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• pp.31-36
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• 2001

Optimal operation in chromatography is needed to save operation time and the solvent used in multiple chromatographic runs. To this end, many simulation studies of chromatography process have been performed. The relationship between the distribution coefficient and the ionic strength is important in gradient elution ion chromatography. Experimental runs and computer simulations were carried out under linear gradient elution condition in order to compare the experiments and the simulation. Experiments were performed with formic acid under isocratic conditions to determine the simulation equation parameters. Computer simulation was based on three equations which related distribution with ionic strength as follows; K=${\alpha}$I(sup)-${\beta}$, K=A+BI+Cl$^2$and K=y(sub)0+A$_1$$.$e(sup)(-I/m$_1$). The effects of gradient slope on the chromatograms are discussed, and good agreement between the experimental and the simulated results is shown.

• Food Science and Biotechnology
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• 제18권2호
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• pp.407-412
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• 2009

In this study, a rapid and efficient concentrating procedure that can be used for detecting viruses in vegetables was developed. The Sabin strain of poliovirus type 1 was used to evaluate the efficiency of virus recovery. The procedure included: (a) elution with 0.25 M threonine-0.3 M NaCl pH 9.5; (b) polyethylene glycol (PEG) 8000 precipitation; (c) chloroform extraction; (d) 2$^{nd}$ PEG precipitation; (f) RNA extraction; (g) reverse transcription-polymerase chain reaction (RT-PCR) combined with semi-nested PCR. The overall recoveries by elution/concentration were 29.0% from cabbage and 13.7% from lettuce. The whole procedure usually takes 18 hr. The overall detection sensitivity was 100 RT-PCR units of genogroup II norovirus (GII NoV)/25 g cabbage and 100 RT-PCR units of GII NoV/10 g lettuce. The virus detecting method developed in this study should facilitate the detection of low levels of NoV in cabbage and lettuce.

• Environmental Engineering Research
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• 제15권1호
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• pp.41-48
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• 2010

This paper describes the influence of nutrient salts eluted from the bottom of a closed water area where polluted sediment has been deposited by inflowing river water. The elution pattern was monitored at our experimental facility. Both the sediment pore water and water above the bottom were sampled using a dialyzer sampler (peeper). The pore water of the eutrophicated sediment contained a large amount of nutrient salts, and the effect of elution was confined to a limited area of the bottom surface. The nutrient concentration of the sediment pore water was closely related to both the water temperature and dissolved oxygen (DO) concentration. The eluted nutrients from the sediment provided a source for phytoplankton and algae growth. This experimental data indicated that the water quality of the surface was not directly connected to the eluted nutrient salts, while it was indirectly affected by the total ecosystem, including all the organisms within an area and their environment.