• 생물환경조절학회지
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• 제30권4호
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• pp.257-262
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• 2021

유인 방법은 딸기 묘를 생산하는 전통적인 방법이다. 그러나 딸기 묘를 유인하여 번식하는 방법은 오랜 노동시간이 있어야 하는 노동 집약적인 방법이다. 삽목 방법은 상대적으로 유인 방법에 비해 노동 기간과 작업 시간이 짧으므로 유인의 효과적인 대안으로 주목되어왔다. 이 연구는 유인과 삽목 사이의 노동 기간, 작업 시간 및 묘의 생육을 조사하기 위해 수행되었다. '매향' 딸기는 두 개 동의 딸기 육묘용 유인 온실과 삽목 온실에서 각각 재배되었다. 유인 방법(유인 작업, 모주 제거, 자묘 간 분리) 및 삽목 방법(삽수 채묘, 저장 전 처리, 삽목 작업)에 대한 주요 작업 시간을 측정했으며, 유인은 158일 동안 6개의 작업이 필요하며, 삽목은 113일 동안 4개의 작업이 필요했다. 유인은 삽목보다 작업 시간이 더 많이 필요했다. 유인과 삽목묘 육묘 방법에 따른 유의적인 차이가 없었다. 이러한 결과는 묘소질의 손실 없이 육묘 동안 유인 방법보다 삽목 방법이 더 많은 노동 기간과 작업 시간을 절약할 수 있음을 의미한다.

• The Plant Pathology Journal
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• 제33권6호
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• pp.561-571
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• 2017

Prospecting of local grapevine (Vitis vinifera L.) germplasm revealed that Tunisia possesses a rich patrimony which presents diversified organoleptic characteristics. However, viral diseases seriously affect all local grapevine cultivars which risk a complete extinction. Sanitation programs need to be established to preserve and exploit, as a gene pool, the Tunisian vineyards areas. The presence of the Grapevine leafroll associated virus-3 (GLRaV-3), Grapevine stem pitting associated virus (GRSPaV) and Grapevine virus A (GVA), were confirmed in a Tunisian grapevine cultivar using serological and molecular analyses. The association between GRSPaV and GVA viruses induces more rugose wood symptoms and damages. For this reason the cleansing of the infected cultivar is highly advisable. Direct and recurrent somatic embryos of cv. 'Hencha' were successfully induced from filament, when cultured on $Ch{\acute{e}}e$and Pool (1987). based-medium, enriched with $2mg1^{-1}$ of 2,4-dichlorophenoxyacetic acid and $2.5mg1^{-1}$ of Thidiazuron, after 36 weeks of culture. After six months of acclimatization, RT-PCR carried on 50 somaplants confirmed the absence of GVA, GRSPaV as well as GLRaV-3 viruses in all somaplants. Ampelographic analysis, based on eight OIV descriptors, was carried out on two years acclimated somaplants, compared to the mother plant. Results demonstrated that the shape and contours of 46 somaclones leaves are identical to mother plant leaves and four phenotypically off-type plants were observed. The healthy state of 100% 'Hencha' somaclones and the high percentage of phenotypically true-to-type plants demonstrate that somatic embryogenesis is a promising technique to adopt for grapevine viruses elimination.

• Journal of Plant Biotechnology
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• 제5권4호
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• pp.239-244
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• 2003

A protocol has been developed for rapid large scale clonal propagation of an aromatic endangered medicinal plant, Hemidesmus indicus R. Br. with the elimination of the problems such as premature leaf fall and callus formation during caulogenesis and rhizogenesis. Multiple shoots were induced from shoot tip and nodal explants on Murashige and Skoog (MS) medium supplemented with 1 mg/L Benzylaminopurine (BAP) and 0.5 mg/L Napthaleneaceticacid (NAA). Addition of 15 mg/L adenine sulphate to the above medium checked leaf abscission completely, reduced the time required for caulogenesis and restored morphogenetic potential after several subcultures. The in vitro grown propagules were rooted in 1/2 MS medium supplemented with 2 mg/L Indolebutyric acid (IBA) +1 mg/L NAA and sucrose 0.7% (w/v). Addition of charcoal at 100 mg/L to the rooting medium quickened root initiation with a complete check on callus formation. The effect of sucrose concentration on both caulogenesis and rhizogenesis was also studied. The resultant plantlets were acclimatized and grown in fields where ninety eight percent of the rooted shoots survived and grew normally. The estimation of the secondary metabolite content in the shoots of the regenerated plant and the mother plant indicated that the concentration of the three secondary metabolites lupeol, vanillin and rutin was similar.