To improve the quality and prolongation of chopped ginger, they were treated with NaCl, citric acid, ascorbic acid alone or combination of them at $5^{\circ}C$. The browning and stink of chopped ginger were prevented by adding 10% NaCl, $0.5{\sim}2.0%$ citric acid, but the control became severely browned and deteriorated after 1 month storage. The application of 0.5% citric acid maintained citrine color and decreased the activity of polyphenol oxidase(PPO) more significantly compared to 5.0% NaCl or 0.5% ascorbic acid. Ascorbic acid treatment become browned at $1.0{\sim}2.0%$ and easily deteriorated compared to citric acid. Citric acid(0.5%) or ascorbic acid (0.5%) treatment was largely variation of pH compared to NaCl(5.0%)+citric acid(0.5%) or NaCl(5.0%)+ascorbic acid(0.5%) treatment during 8 month storage. The activity of PPO in NaCl(5.0%)+ascorbic acid(0.5%) treatment was elevated after 4 month storage. In chopped ginger, NaCl(5.0%)+citric acid(0.5%)+ascorbic acid(0.25%) treatment was more effective than 5.0%+0.1%+0.1%, 5.0%+0.5%+0.1% during 12 month storage.
Effects of $CO_2$ concentration (500, 800, 1,000 and 1,500 ppm) on the initiation and development of fruit body of Grifola frondosa on sawdust cultivation were studied. Optimum concentrations of carbon dioxide for the initiation and development of the fruit body showed the ranges from 500 to 800 ppm. Fruit body initiation was accelerated at lower than 800 ppm $CO_2$ exposure but the maturing of the fruit body was not influenced by above treated $CO_2$ concentrations. The higher ratio of primordial formation, faster fruit body initiation and higher yield were obtained at below 800 ppm of $CO_2$ level, whereas over 1,000 ppm of $CO_2$ levels showed abnormal and lower quality of fruiting bodies. Based on the above results, it is concluded that the favorable $CO_2$ level for bag culture of G. frondosa was below 800 ppm.
Seo, Young-Jin;Kim, Jong-Su;Kim, Sun-Hwa;Kim, Mi-Yeon;Jeong, Yong-Jin;Seong, Gi-Un;Chung, Shin-Kyo
Food Science and Preservation
/
v.22
no.6
/
pp.804-810
/
2015
Salvia miltiorrhiza has been used for treating heart and liver disease. In the present study, the influences of temperature on photosynthetical capacity of S. miltiorrhiza under controlled cultivation environment using growth chamber were investigated because of providing information about growth and secondary metabolite synthesis. And effect of harvesting time on growth properties and constituents such as salvianolic acid B, cryptotanshinone, tanshinone I, tanshinone IIA were evaluated. Maximum photosynthesis rate ($5.102{\mu}mol\;CO2/m2/s$) and net apparent quantum yield ($0.147{\mu}mol\;CO2/m2/s$), stomatal conductance (0.035 mmol/m2/s) and water use efficiency ($7.108{\mu}mol\;CO2/mmol\;H2O$) was highest at $20^{\circ}C$. Results of chlorophyll fluorescence showed that elevated temperature had contributed to reduce a quantum yield and electron flux in photosystem. This result demonstrated that favorable temperature condition was determined at $20^{\circ}C$. Contents of salvianolic acid B, cryptotanshinone, tanshinone I and tanshinone IIA was highest in root sample harvested at 20 March, whereas growth and yield of S. miltiorrhiza had no significant differences with harvesting time. Therefore, this study shows that temperature play an important role in photosynthetic activity and harvesting time have influence upon accumulation of constituents in root of S. miltiorrhiza.
This study was conducted to find out the influence of single and/or repeatedly combined application of cellulose and chlorotharonil(TPN) on bacterial population and dissipation of these chemicals by microorganism in the soil suspension culture. 1. The number of total bacteria and Gram-negative bacteria were rapidly increased for 1st week, after the time, decreased in the single application but maintained in repeatedly combined application for 5th week and that were higher at combined application of TPN and cellulose than at single application of these. 2. TPN-degrading bacteria were gradually increased by the repeated application of TPN, and elevated the increasing extents of those with the repeatedly combined application of cellulose with TPN. 3. Cellulose-degrading bacteria showed the peak at 1st week, and was increased in the repeated application of cellulose, but the bacteria was decreased in the repeatedly application of TPN with cellulose. 4. The dissipation of TPN were faster at the combined application of both than at the single application of TPN, wheras that was delayed by the repeatedly combined application. 5. The content of reducing sugar was rapidly increased at 1st week and after 4th week except for the TPN single application, that was higher in the combind application than the cellulose single, but there was no difference between them.
In order to assess seasonal and geographical characteristics of pollutant Escherichia coli, we investigated its distribution in Nakdong River mouth and Busan coastal water from February 2013 to November 2015. The coastal area was divided into five different zones (I-V) based on the pollutant level and geographical characteristics. During the study periods, water temperature and salinity varied from 7.50 to 27.64℃ and 16.82 to 34.82 psu, respectively. The annual water temperature variation was characterized in temperate zone. The salinity was significantly (p<0.05) decreased in zone IV and zone III after heavy rain during summer season in 2014, resulting led to elevated E. coli biomass. The highest colony formation of E. coli was recorded at 6,000 cfu l-1 during autumn at station 1 (zone I). On the other hands, during all seasons of 2015, E. coli abundances were kept to be low level in zone III. The E. coli was not significantly (p>0.05) correlated with water temperature. However, the salinity was significantly (r=-0.53, p<0.05) correlated with the E. coli, implying that salinity plays a crucial role in the proliferation of E. coli. Consequently, E. coli in western Busan coastal water might have been significantly promoted by pollutant sources from Nakdong Rive discharge during the spring and summer rainy seasons depending on annual rainfall variations. On the other hands, E. coli in station 1 (i.e., Suyeong Bay) was obviously high due to influences of discharge water from municipal wastewater treatment plant. However, there was no clear seasonality of E. coli.
Recently, more researches have been actively performed for the assessment of material degradation and residual-life of elevated temperature plant components, as some of domestic fossil power plants become older than 30 years. In this paper, results of on_site residual life assessment are reported for main steam pipes of Youngwol power station #2 which have operated since 1965. For critical weld locations such as butt welds branch welds, Y_sections and a T-section, replication technique and hardness measurement technique were employed for life_assessment. When cracks were detected by conventional NDT tests, crack growth life was calculated using a computer code. On the other hand, for matrix of pipes, residual life was quantitatively estimated by an analytic method and material degradation was estimated qualitatively using diameter measurement data and grain-boundary etching method. Also, direction in further improvement of on-site life assessment techniques are proposed.
This research was conducted to determine the effects of four different substrates, expanded rice hulls (ERH), commercial substrates for strawberries (CSS), clay sand (CS), and loamy sand (LS), on the inhibition of anthracnose crown rot (ACR) in strawberry. Mother plants of 'Seolhyang' strawberry were transplanted into an elevated bed in March, 2013 and March, 2014 and the runners connecting mother plants and daughter plants were cut in early August of both years. After separation, growth characteristics of the daughter plants were measured and then each daughter plant was inoculated with conidial suspensions of Colletotrichum fructicola, one of several species of Colletotrichum that causes ACR in strawberries. The incidence of ACR as influenced by the different substrates was investigated in both years. The daughter plants grown on CSS had the highest values for shoot height, leaf area, and fresh weight. Those grown on ERH and LS substrates also displayed good above-ground growth characteristics except for fresh weight, but the daughter plants grown on CS had the poorest above-ground growth characteristics. The ERH and CS treatments resulted in the highest number of primary roots and the greatest root weight. The CSS-grown daughter plants had the highest ACR disease index, followed by the CS and LS treatments, but there were no significant differences among the three substrates. However, the ERH-grown daughter plants had a markedly lower ACR disease index on October 11, 2013 and October 7, 2014. The CSS-grown daughter plants had high nitrogen and potassium contents and low calcium content, whereas the ERH-grown daughter plants had low nitrogen levels and high silicon levels. The results of this study provide basic information on the ability of the different substrates tested to provide disease suppression of ACR in the propagation of strawberry transplants.
Kim, Jae Jun;Hyun, Kwanyong;Park, Jae Kil;Moon, Seok Whan
Journal of Chest Surgery
/
v.48
no.5
/
pp.335-344
/
2015
Background: A raised carcinoembryonic antigen (CEA) may be associated with significant pathology during the postoperative follow-up of lung adenocarcinoma. Methods: We reviewed the medical records of 305 patients who underwent surgical resections for primary lung adenocarcinoma at a single institution between April 2006 and February 2013. Results: Preoperative CEA levels were significantly associated with age, smoking history, pathologic stage including pT (pathologic tumor stge), pN (pathologic nodal stage) and overall pathological stage, tumor size and differentiation, pathologically positive total lymph node, N1 and N2 lymph node, N2 nodal station (0/1/2=1.83/2.94/7.21 ng/mL, p=0.019), and 5-year disease-free survival (0.591 in group with normal preoperative CEA levels vs. 0.40 in group with high preoperative CEA levels, p=0.001). Preoperative CEA levels were significantly higher than postoperative CEA levels (p<0.001, Wilcoxon signed-rank test). Postoperative CEA level was also significantly associated with disease-free survival (p<0.001). A follow-up serum CEA value of >2.57 ng/mL was found to be the appropriate cutoff value for the prediction of cancer recurrence with sensitivity and specificity of 71.4% and 72.3%, respectively. Twenty percent of patients who had recurrence of disease had a CEA level elevated above this cutoff value prior to radiographic evidence of recurrence. Postoperative CEA, pathologic stage, differentiation, vascular invasion, and neoadjuvant therapy were identified as independent predictors of 5-year disease-free survival in a multivariate analysis. Conclusion: The follow-up CEA level can be a useful tool for detecting early recurrence undetected by postoperative imaging studies. The perioperative follow-up CEA levels may be helpful for providing personalized evaluation of lung adenocarcinoma.
Lee, M.T.;Lin, W.C.;Lin, L.J.;Wang, S.Y.;Chang, S.C.;Lee, T.T.
Asian-Australasian Journal of Animal Sciences
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v.33
no.7
/
pp.1113-1125
/
2020
Objective: This study was investigated the effects of dietary supplementation of Antrodia cinnamomea fermented product on modulation of antioxidation, anti-inflammation, and lipid metabolism in broilers. Methods: Functional compounds and in vitro antioxidant capacity were detected in wheat bran (WB) solid-state fermented by Antrodia cinnamomea for 16 days (FAC). In animal experiment, 400 d-old broiler chickens were allotted into 5 groups fed control diet, and control diet replaced with 5% WB, 10% WB, 5% FAC, and 10% FAC respectively. Growth performance, intestinal microflora, serum antioxidant enzymes and fatty acid profiles in pectoral superficial muscle were measured. Results: Pretreatment with hot water extracted fermented product significantly reduced chicken peripheral blood mononuclear cells death induced by lipopolysaccharide and 2,2'-Azobis(2-amidinopropane) dihydrochloride. Birds received 5% and 10% FAC had higher weight gain than WB groups. Cecal coliform and lactic acid bacteria were diminished and increased respectively while diet replaced with FAC. For FAC supplemented groups, superoxide dismutase (SOD) activity increased at 35 days only, with catalase elevated at 21 and 35 day. Regarding serum lipid parameters, 10% FAC replacement significantly reduced triglyceride and low-density lipoprotein level in chickens. For fatty acid composition in pectoral superficial muscle of 35-d-old chickens, 5% and 10% FAC inclusion had birds with significantly lower saturated fatty acids as compared with 10% WB group. Birds on the 5% FAC diet had a higher degree of unsaturation, followed by 10% FAC, control, 5% WB, and 10% WB. Conclusion: In conclusion, desirable intestinal microflora in chickens obtaining FAC may be attributed to the functional metabolites detected in final fermented product. Moreover, antioxidant effects observed in FAC were plausibly exerted in terms of improved antioxidant enzymes activities, increased unsaturated degree of fatty acids in chicken muscle and better weight gain in FAC inclusion groups, indicating that FAC possesses promising favorable mechanisms worthy to be developed.
In this study, ethanolic extracts from Hericium erinaceum cultivated with Artemisia capillaris (HEAC) were assessed for their ability to lower the cholesterol levels of male Sprague-Dawley rats fed a high-fat diet. Rats were randomly subdivided into seven test groups. Each group contained eight rats fed a high-fat diet during a growth period lasting 4 wk. Supplementation with the extracts was performed once a day for 2 wk after the high-fat diet. The control group (rats fed a high-fat diet) showed a high efficiency ratio (feed efficiency ratio) value compared to the normal group. Biochemical parameters, including total cholesterol (TC), low-density lipoprotein-cholesterol (LDL-c), and triglyceride (TG) levels dramatically increased in the control group compared to the normal group. High-density lipoprotein-cholesterol (HDL-c) content in the control group was also significantly lower relative to the normal group. Two positive control groups, treated with simvastatin and atorvastatin, had lowered TC, LDL-c, and TG levels, and increased HDL-c content compared to the control group. Treatment with the tested extracts, including HEAC, ethanolic extracts from Hericium erinaceum, and ethanolic extracts from Artemisia capillaris reduced TC, LDL-c, and TG levels and elevated HDL-c content in the hyperlipidemia rats. The atherogenic index and cardiac risk factor values for the HEAC-treated group were 0.95 and 1.95, respectively. Simvastatin- and atorvastatin-treated groups showed atherogenic index values of 1.56 and 1.69, respectively, and cardiac risk factor values of 2.56 and 2.69, respectively. These results show HEAC possesses an ability to cure hyperlipidemia in rats and may serve as an effective natural medicine for treating hyperlipidemia in humans.
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