• The Plant Pathology Journal
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• v.16 no.5
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• pp.269-279
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• 2000

Alfalfa mosaic alfamoviruses(AIMV) were isolated from infected potato (Solanum tuberosum) and azuki bean (Paseolus angularis) in Korea. Two AIMV isolated from potatoes were named as strain KR (AIMV-KR1 and KR2) and AIMV isolated from azuki bean was named as strain Az (AIMV-Az). Each isolated AIMV strain was characterized by using their host ranges, symptom developments, serological relations and nucleotide sequence analysis of coat protein (CP) gene. Strains KR1, KR2, and Az were readily transmitted to 20 of 22 inoculated plant species including bean, cowpea, tomato, tobacco, and potato. AIMV-KR1 and KR2 produced the typical symptoms like chlorotic or necrotic spots in Chenopodium quinoa and Solanum tuberosum cv. Superior. AIMV-Az caused bright yellow mosaic symptom and leaf malformation in Nicotiana glauca, which were different from the common mosaic symptom caused by AIMV-KR1 and KR2. Electron microscope observation of purified virus showed bacilliform virions containing a single-stranded plus-strand RNAs of 3.6, 2.6, 2.0 and 0.9 kbp in length, respectively, similar in size and appearance to those of Alfamovirus. In SDS-PAGE, the coat protein of the two viruses formed a consistent band that estimated to be about 24kDa. The CP genes of the AIMV strains, KR1, KR2, and Az have been amplified by RT-PCR using the specific primers designed to amplify CP gene from viral RNA-3, cloned and sequenced. Computer aided analysis of the amplified cDNA fragment sequence revealed the presence of a single open reading frame capable of encoding 221 amino acids. The nucleotide and peptide sequence of viral CP gene showed that strain KR1, KR2, and Az shared highest nucleotide sequence identities with AIMV strain 425-M at 97.7%, 98.2%, and 97.2%, respectively. CP gene sequences of two strains were almost identical compared with each other. Altogether, physical, serological, biological and molecular properties of the purified virus.

• Journal of Conservation Science
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• v.28 no.2
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• pp.119-129
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• 2012

Excavated textiles provide very important research data on the costume culture of the Joseon dynasty. In particular, dyed textiles are indispensable for textile conservation research and for restoration of remains as well as for general costume culture research. Unfortunately, a prolonged burial environment causes the colors to change and gradually fade after excavation. Therefore, it is very difficult to identify the original color. In this study, natural dyed samples of red, yellow, purple and blue were prepared and analyzed using HPLC-PDA. Dyes of colorants extracted from excavated textile remains were analyzed by HPLC. In addition, mordants were analyzed using (SEM-EDX) in order to estimate the original color. The 16~17th Century's three samples were analyzed, sample 1, and 2 from Eunjin Song's Song Mun-Chang excavated at the Songchon-dong in Daejeon, and sample 3 from Yeosan Song's Song, Hee-Jong excavated at the Mokdal-dong in Daejeon. From the HPLC results, alizarin, purpurin, and indigo were detected on sample 1, alizarin and purpurin on sample 2, ellagic acid and indigo on sample 3. Therefore they were dyed with madder and indigo (sample 1), madder (sample2), pomagranted and indigo (sample 3). Al mordant was identified on three samples.

• Proceedings of the Korean Vacuum Society Conference
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• 2012.02a
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• pp.433-433
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• 2012

As the requirement in patterning geometry continuously shrinks down, the termination of etch process at the exact time became crucial for the success in nano patterning technology. By virtue of real-time optical emission spectroscopy (OES), etch end point detection (EPD) technique continuously develops; however, it also faced with difficulty in low open ratio etching, typically in self aligned contact (SAC) and one cylinder contact (OCS), because of very small amount of optical emission from by-product gas species in the bulk plasma glow discharge. In developing etching process, one may observe that coupon test is being performed. It consumes costs and time for preparing the patterned sample wafers every test in priority, so the coupon wafer test instead of the whole patterned wafer is beneficial for testing and developing etch process condition. We also can observe that etch open area is varied with the number of coupons on a dummy wafer. However, this can be a misleading in OES study. If the coupon wafer test are monitored using OES, we can conjecture the endpoint by experienced method, but considering by data, the materials for residual area by being etched open area are needed to consider. In this research, we compare and analysis the OES data for coupon wafer test results for monitoring about the conditions that the areas except the patterns on the coupon wafers for real-time process monitoring. In this research, we compared two cases, first one is etching the coupon wafers attached on the carrier wafer that is covered by the photoresist, and other case is etching the coupon wafers on the chuck. For comparing the emission intensity, we chose the four chemical species (SiF2, N2, CO, CN), and for comparing the etched profile, measured by scanning electron microscope (SEM). In addition, we adopted the Dynamic Time Warping (DTW) algorithm for analyzing the chose OES data patterns, and analysis the covariance and coefficient for statistical method. After the result, coupon wafers are over-etched for without carrier wafer groups, while with carrier wafer groups are under-etched. And the CN emission intensity has significant difference compare with OES raw data. Based on these results, it necessary to reasonable analysis of the OES data to adopt the pre-data processing and algorithms, and the result will influence the reliability for relation of coupon wafer test and whole wafer test.

• Journal of Conservation Science
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• v.34 no.5
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• pp.433-442
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• 2018

In this study, metal properties were compared by preparingthree iron knives from steel ingots produced via traditional iron-making, and ingot which jointed the steel of modern times. Metal microscope and SEM-EDS analysis revealed fine ferrite and pearlite structures of the hypo-eutectoid steel of Fe-C alloys. All samples also exhibited martensite on the blade of the knife. By Vicker's hardness analysis, the hardness of the sand iron knife (K1) was 533.38 HV, sand iron-nickel steel knife (K3) was 514.8 HV, and sand iron-carbon steel knife (K2) was 477.02 HV. The mass reduction due to wear was 0.058% for K1, 0.059% for K3, and 0.144% for K2. EPMA(Electron probe micro-analyzer) analysis of the surface pattern of the specimens confirmed that the patterns were exposed due to differences in the content of C or the chemical composition. Additional research on heat treatment processes is needed to increase the abrasion resistance of blades. Traditional steel ingots could produce high-quality steel if combined with nickel steel.

• Proceedings of the Korean Radioactive Waste Society Conference
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• 2005.06a
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• pp.129-136
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• 2005

There should not be ion exchange resin particles in S/G sludge. The suspicious spherical resin particles observed in S/G sludge sample were characterized for particle size distribution under optical microscope using the micro-technique, for element analysis by the electron probe micro analysis (EPMA), and for molecular identification by the IR spectroscopy The particle sizes are distributed from 1 to 200 ${\mu}m$ for the sludge, while 40 to 500 ${\mu}m$ for the spherical resin particles. The results of the elemental analysis showed different major impurities: Si, Al, Mn, Cr, Ni, Zn and Ti for the sludge particles, while Si, Cu, Zn for the spherical resin particles. However, both particles contain Fe as a matrix of hematite ($Fe_{3}O_4$). IR spectrum of the spherical particles was quite different from that of ion exchange resins used in S/G system. These results indicate that the spherical particles are not related to ion exchange resin particles and formed by the process of the sludge formation.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.16 no.4
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• pp.2391-2400
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• 2015

Weather proof steels are used for steel bridges due to its high corrosion resistance under atmospheric conditions. However, instead of forming stabilized rust layers, general rust occurs on weather proof steels under high humidity condition close to seawater or shady places. In Japan, therefore, they perform rust stabilization treatment instead of unpainted treatment due to severe atmospheric conditions. However, most of domestic weather proof steels were constructed unpainted in the form of closed box-girder, which makes the periodical repetition of dry and wet hard to occur. For the steel bridges constructed on the Han river, the evaporation of water, dew condensation due to temperature change, and stagnant water due to rain affect harmfully on the formation of passive film on weather proof steels. Thus, in this research, in order to analyze corrosion properties inside the closed box-girder for the unpainted weather proof steel bridge in the waterworks safety zone, multiple ways of analysis such as observation with eyes, cellophane-tape test, steel thickness measurement, surface corrosion potential measurement, electron microscope analysis, and X-ray diffraction analysis of the rust were performed. As a result, unstable rust layer was observed inside the closed box-girder, and severe corrosion was observed on the top and bottom of the flanges due to the effects of stagnant water caused by rain, dew condensation, and de-icing materials.

• Korean Journal of Food Science and Technology
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• v.34 no.2
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• pp.207-212
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• 2002

This study was to examine the characteristics in the texture of acorn Mooks (acorn starch jelly) with different conditions to prepare acorn starch. By texture profile analysis, hardness and chewiness with 50% deformation were increased with increasing soaking days. Hardness of acorn Mook, measured by texture analyzer, showed significantly difference between the samples, and was closely related to the content of non-starchy substances and their intrinsic viscosity. The observation of microstructure through a scanning electron microscope (SEM) showed that acorn Mook showed finer and more fibrous structure than control(0-0), sample without soaking of acorn and sediments. Sensory characteristics of acorn Mook were affected by soaking treatment. The result of acceptance test on acorn Mooks indicated that the color and hardness increased with the increase of soaking days of acorn nuts and soaking times of sediments. There was a little difference between the samples in astringency taste. Their overall acceptability also increased except for 4-2 sample (i.e., soaking of acorn, 4 days; soaking of sediments, 2 times) and 4-3 sample (i.e., soaking of acorn nuts, 4 days; soaking of sediments, 3 times). As the results, the color, light brown, and the taste, a little astringency with consistency, were important factors of acorn Mook.

• Journal of the Korea institute for structural maintenance and inspection
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• v.17 no.2
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• pp.94-100
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• 2013

In this paper, Machinery analysis was conducted, in order to predict highest exposure temperatures and the analyze fire damage in the case of fire on reinforced concrete structure. After analyzing differential thermal of reference materials in accordance with temperature of concrete reference core specimen, it turned out that powerful endothermic peak came resulting from evaporation of capillary water and get water untill $200^{\circ}C$, another endothermic peak came resulting from decomposition of calcium hydroxide at $520^{\circ}C$, and then mass of reference materials remarkably decreased due to endothermic reaction. Another powerful endothermic reaction came after decomposition of calcite at $720^{\circ}C$. After analyzing X-ray diffraction of reference materials in accordance with temperature of concrete reference core specimen, it turned out that calcium hydroxide existed until the temperature of $400^{\circ}C$, but CH almost disappeared and CaO appeared from $600^{\circ}C$. The production increased in proportion to the temperature. This is because that calcium hydroxide and calcite are decomposed and CaO is produced when the temperature of concrete increases with fire. It is estimated that calcium hydroxide and calcite are utterly decomposed and peak disappears, and peak of CaO is remarkably formed instead, at the temperature of $700-800^{\circ}C$.

• Journal of the Korea Concrete Institute
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• v.23 no.1
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• pp.121-127
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• 2011

In this study, possible use of indigenous natural loess (Hwangtoh) as a new binding material via geopolymerization process is examined. Hwangtoh pastes with four different mix proportions of varying alkali liquid concentrations (6 M, 8 M) and the constituents of the binder as well as the alkali liquid at a constant liquid-to-binder ratio of 0.55 were prepared. Analysis of the natural loess (Hwangtoh) paste was carried out as follows : 1) Measurement of compressive strength and weight of cubic specimens versus curing time; 2) Analysis by X-ray diffraction (XRD) and scanning electron microscope (SEM) about reaction product; 3) Porosity analysis of hardened Hwangtoh paste. The result showed that it is possible to prepare Hwangtoh paste with 29.1 MPa at the age of 7 day by using alkali solution (made as 1 : 4.5 the mass ratio of liquefied $Na_2SiO_3$ and NaOH solution and applying the curing temperature of $60^{\circ}C$). Compressive strength development with respect to the degree of moisture evaporation from the paste seems to be independent of curing temperature. Therefore, it seems that higher early strength of the paste specimens cured at higher temperature can be attributed to both higher rate of reaction and moisture evaporation.

• Journal of the Mineralogical Society of Korea
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• v.30 no.4
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• pp.179-186
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• 2017

This study investigated the mineralogical characteristics of lime mortars used in pointing of Namhansanseon Yeojang from Joseon dynasty. Polarization microscopy revealed quartz, feldspar, mica, pyroxene and opaque minerals in the lime mortars. XRD analysis also confirmed clay minerals chlorite and kaolin. As a result of particle size analysis of lime mortars, the particle size distribution was wide and size was not uniform. 60% of samples were corresponded to the sand area. The chemical components detected from XRF analysis, 8.71-11.18 % of Ca as the main component of lime and $SiO_2$, $Al_2O_3$, and $Fe_2O_3$ in soil minerals were main components. The lime mortars showed an endothermic peak due to decarbonization reaction of $CaCO_3$ at $750^{\circ}C$ and weight reduction rate of 10%. The microstructures were agglomerated amorphous and observed rhombohedral calcite crystals by scanning electron microscope. It is considered that the pointing of Yeojang is a mortar mixed with lime and soil. In addition the Hanbongseong Yeojang was constructed using the same materials and construction technique because the minerals composition is not different according to the Yeojang location and use.