• Title/Summary/Keyword: eggs development

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Effect of Ultra-sonication Treatment on the Quality Characteristics of Baked Eggs

  • Kang, Geunho;Seong, Pil-Nam;Cho, Soohyun;Ham, Hyoung-Joo;Kang, Sun-Moon;Kim, Dayae;Park, Beom-Young;Ba, Hoa Van
    • Food Science of Animal Resources
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    • v.36 no.4
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    • pp.458-462
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    • 2016
  • The effect of ultra-sonication on quality characteristics and flavor of baked eggs was studied. One hundred and twenty eggs were cooked and assigned to six treatments groups (n=20 each) that were then soaked in saline solution at various concentrations (5, 10 and 18%) with/or without further ultra-sonication treatment at 35 kHz for 1 h. The pH values were lower in the ultra-sonicated samples in comparison with the non-ultra-sonicated samples (p<0.05). The values for texture traits were higher in the samples soaked in 10% saline solution with ultra-sonication in comparison with other remaining treatments or control (p<0.05). The sodium content in samples soaked in 10% saline solution with ultra-sonication was similar to that of the ones soaked in 18% saline solution without ultra-sonication. The higher flavor scores were also given for the ultra-sonicated samples in comparison with the control or non-ultra-sonicated ones. These results suggest that the application of ultra-sonication may produce a faster sodium penetration into baked eggs, simultaneously improves some textural traits (e.g., hardness) as well as flavor of the products.

Occurrence of Eggs and Larvae of Blackfin Flounder Glyptocephalus stelleri (Pleuronectidae, Pisces) off Wangdol-cho, East Sea (동해 왕돌초 주변해역에서 기름가자미 Glyptocephalus stelleri (가자미과, 어상강) 어란과 자어 출현)

  • Lee, Hae Won;Lee, Soo Jeong;Yang, Jae Hyung;Lee, Jae Bong;Cha, Hyung Kee;Kim, Jin-Koo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.47 no.5
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    • pp.654-658
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    • 2014
  • We report the first identification of a spawning ground of the blackfin flounder Glyptocephlaus stelleri near the Wangdol-cho sea mountains, located in the southern East Sea. Eggs and larval fish of G. stelleri were collected during April and June, 2014, when an abundance of eggs was found in the southern area of Wangdol-cho. Our findings suggest that G. stelleri prefers to spawn in the vicinity of the off-shore sea mountains, where the temperature is between 10 and $12^{\circ}C$ and the water depth is 100 m, rather than inshore.

A Study on Stroage of Chicken Eggs from Poultry Farms (식란의 보전성에 관한 연구)

  • 조태행;인영민;정갑수;남궁선
    • Journal of Food Hygiene and Safety
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    • v.4 no.1
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    • pp.29-36
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    • 1989
  • In order to investigate the Storage time of chicken eggs, several physico-chemical tests from chicken eggs store at 5, 13 and 27$^{\circ}C$ were examined. Egg samples were collected from six poultry farms. Egg stored at 5$^{\circ}C$ based on the depth of air cell and specific gravity, were all acceptable until 17 days ; on the egg yolk coefficient and pH of the egg white and egg yolk until about 10 days. Egg stored at 13$^{\circ}C$, based on the depth of air cell. were acceptable by about 10 days of storage, but on the other physico-chemical tests by about 7 days. Egg samples stored at room temperature(about 27$^{\circ}C$) base on the depth of air cell, were acceptable by about 5 days of storage ; on the specific gravity by 4 days ; and on the egg yolk coefficient and pH of the egg yolk and egg white by 3 days. The results of this study showed that egg stored at 5$^{\circ}C$ were considered acceptable by 10 days of storage ; at 13$^{\circ}C$ by 7 days ; at room temperature (27$^{\circ}C$) by 3 days.

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Changes in the Titer of Protein and Cholesterol Content in Non-Diapause, Artificially Diapause Terminated and Diapause Eggs of Silkworm, Bombyx mori.

  • Moorthy, S.M.;Krishnan, N.;Bhattacharya, Tanmay;Chaudhuri, A.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.15 no.2
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    • pp.165-169
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    • 2007
  • A differential specific pattern of variation in the metabolism of protein and cholesterol was noticed in non -diapause and diapause eggs due to the significant differences in embryonic development. The rate of metabolism was different due to specific demands of such metabolites during active embryogenesis and maintenance of diapause respectively. In general, the metabolic rate was found to be accelerated in non- diapause eggs just after egg deposition, while it was very slow in diapause eggs. When the diapause eggs were treated with hydrochloric acid within 16-20 hrs, the rate of turnover was found to very similar to non- diapause eggs, though the base level of protein and cholesterol was recorded to be different.

Parthenogenetic development of mouse eggs I. Parthenogenetic activation by ethanol and hyaluronidase treatments (생쥐 난자의 단위발생에 관한 연구 I. Ethanol 및 hyaluronidase처리에 의한 단위발생유기)

  • Lee, Hyo-jong;Ha, Dae-sik;Kang, Tae-young;Choi, Min-cheol
    • Korean Journal of Veterinary Research
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    • v.32 no.4
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    • pp.663-669
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    • 1992
  • This experiment was carried out to find out the best condition for the parthenogenetic activation of mouse eggs by treating ethanol and hyaluronidase. For the parthenogenetic activation of eggs with ethanol, cumulus cell enclosed or denuded eggs were treated with 7% ethanol in D-PBS for 5, 7 or 9 minutes. For the activation of eggs with hyaluronidase, the eggs with cumulus masses were released into D-PBS with 100 unit hyaluronidase and treated for 10, 12 or 13 minutes. All of the treated eggs were incubated in BMOC-3 solution for 5 hours at $37^{\circ}C$ at an atmosphere of 5% $CO_2$ in air. The types of parthenogenetic eggs were morphologically classified into haploid, diploid, immediate cleavage eggs under an inverted microscope. The results obtained in this experiment were summarized as follows ; 1. High activation rate(99%) had been achieved by treating the eggs with 7% ethanol for 7 minutes. 2. With 100 IU hyaluronidase, high activation rate (94%) had been achieved by treating for 12 minutes. 3. The most frequent type of parthenogenetic eggs activated with ethanol or hyaluronidase was haploid (p<0.05). 4. The eggs collected from 18 to 22 hours post HCG injection showed higher activation rate than the eggs collected at 16 hours post HCG injection. 5. No significant difference (p>0.05) in activation rate was shown in strain of mouse and in presence of cumulus cells.

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Effect of Temperature on Embryonation of Ascaris suum Eggs in an Environmental Chamber

  • Kim, Min-Ki;Pyo, Kyoung-Ho;Hwang, Young-Sang;Park, Ki-Hwan;Hwang, In-Gyun;Chai, Jong-Yil;Shin, Eun-Hee
    • Parasites, Hosts and Diseases
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    • v.50 no.3
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    • pp.239-242
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    • 2012
  • The influence of temperature on the development and embryonation of Ascaris suum eggs was studied using coarse sand medium in an environmental chamber with 50% humidity. The time required for development and embryonation of eggs was examined under 3 different temperature conditions, $5^{\circ}C$, $25^{\circ}C$, and $35^{\circ}C$. A. suum eggs did not develop over 1 month at the temperature of $5^{\circ}C$. However, other temperature conditions, $25^{\circ}C$ and $35^{\circ}C$, induced egg development to the 8-cell-stage at days 5-6 after incubation. All eggs examined developed to the 8-cell stage at day 6 after incubation in the sand medium at $25^{\circ}C$. The higher temperature, $35^{\circ}C$, slightly accelerated the A. suum egg development compared to $25^{\circ}C$, and the development to the 8-cell stage occurred within day 5 after incubation. The formation of larvae in A. suum eggs at temperatures of $35^{\circ}C$ and $25^{\circ}C$ appeared at days 17 and 19 after incubation, respectively. These findings show that $35^{\circ}C$ condition shortens the time for the development of A. suum eggs to the 8-cell-stage in comparison to $25^{\circ}C$, and suggest the possibility of accelerated transmission of this parasite, resulting from global warming and ecosystem changes.

Effect of EDTA on the In Vitro Development of Parthenogenetic Mouse Eggs (EDTA가 생쥐 단위발생란의 체외 발달에 미치는 영향)

  • 곽대오;김선구;김영수;박충생
    • Korean Journal of Animal Reproduction
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    • v.17 no.4
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    • pp.365-373
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    • 1994
  • To investigate the effect of EDTA on the in vitro development of parthenogenetic eggs of ICR strain mice, those were cultured in 35mm culture dishes containing NaHCO3-BMOC-3 medium supplemented with 10, 50, 100, or 500$\mu$M of EDTA at 37$^{\circ}C$ for 96hrs. under the atmosphere of 5% CO2 and 95% air. EDTA supplementation of 10, 50, or 100$\mu$M to medium significantly(P<0.01) increase morula and blastocyst formation rate compared with controls in haploid(19.8, 25.9, 39.0% vs. 0.0%). And compared with 10, or 50$\mu$M of EDTA supplementation, significantly(P<0.01) higher morula and blastocyst formation rate resulted from EDTA supplementatin of 100$\mu$M. Both the nuclear number and diameter of blastocysts developed from parthenogenetic eggs were not affected by the morphological types when they were cultured, or the supplementary concentrations of EDTA. The nuclear number of blastocysts developed from haploid, diploid, and immediately cleavaged eggs was 44.8$\pm$1.2, 45.2$\pm$1.5, and 45.4$\pm$1.8, respectively. And the diameter of those eggs ranged 104.4$\pm$1.8, 104.3$\pm$1.2, and 103.8 1.3$\mu$m, respectively.

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Effects of Soil Moisture on Survival of Larger Black Chafer (Holotrichia morosa Waterhouse) Eggs and Larvae (토양 수분함량이 큰검정풍뎅이의 난 및 유충의 생존에 미치는 영향)

  • 김기황
    • Korean journal of applied entomology
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    • v.30 no.1
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    • pp.37-41
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    • 1991
  • Laboratory experiments were conducted to examine the effects of soil moisture on the survival of the larger black chafer(Holotrichia morosa Waterhouse) eggs and larvae. Survival rates of eggs and 1st, 2nd, and 3rd instar larvae were all above 79% at soil moisture of 15% and 25% in sandy loam and clay loam soil, but decreased considerably at 5% and 35%. At these extreme moistures there seem to be differences in survival rates of eggs and larvae between soil textures. Egg development was delayed as soil moisture approached to the lower limit for survival. Older eggs were tolerant to the high moisture stress(33-36 % , clay loam soil), and duration of the stress affected egg development. Feeding of 3rd instar larvae was obviously suppressed at the higher level of soil moisture.

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Microbial and Physicochemical Properties of Liquid Egg during Cold Storage (액란의 냉장저장 중 미생물 및 이화학적 성상)

  • Kang, Geun-Ho;Cho, Soo-Hyun;Seong, Pil-Nam;Park, Beom-Young;Ham, Jun-Sang;Jeong, Seok-Geun;Kim, Dong-Hun;Chae, Hyun-Seok
    • Food Science of Animal Resources
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    • v.31 no.4
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    • pp.557-562
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    • 2011
  • The study was performed to investigate microbial and physicochemical properties of domestic liquid eggs during cold storage. The liquid eggs used in the experiment were whole liquid, liquid egg yolks, and liquid egg whites. All samples were analyzed in summer and winter. The aerobic microorganisms were 1,270-83,300 CFU/g from non-sterilized liquid eggs produced in summer and their numbers increased from those produced in winter (ND, ~4,330 CFU/g). Total coliforms were not observed in non-sterilized whole liquid and non-sterilized liquid egg yolk regardless of season. Total coliforms from nonsterilized products were not detected in liquid egg whites during cold storage. Salmonella sp. was not observed in any of the liquid egg products. However, Pseudomonas sp., Pseudomonas geezennei, Pseudomonas otitidis, and Pseudomonas aeruginosa were identified by 16S rRNA from non-sterilized whole liquid eggs produced in summer. The pH and viscosity of whole liquid eggs and liquid egg whites were not different between the sterilized and non-sterilized treatments during cold storage. These results suggest that managing cross-contamination is necessary when non-sterilized liquid eggs are processed in summer.

Development of Vitrified Bovine Oocytes following Intracytoplasmic Sperm Injection (ICSI)

  • Yeo, H-J;Ock, S-A;Yea, E-H;Lee, H-J;Choe, S-Y;Park, G-J
    • Proceedings of the KSAR Conference
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    • 2001.03a
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    • pp.6-6
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    • 2001
  • Oocyte freezing has become a prevalent source for related reproductive technologies. This study was carried out to evaluate viability of post-thawed bovine oocyte injected DTT-treated sperm following by two different activation stimuli (Group 1, 5 M ionomycin, 5 min + CR1aa, 3 h . 1.9 mM dimetylaminopurine (DMP), 3 h; group 2, ionomycin + 10 $\mu\textrm{g}$/$m\ell$ cycloheximide(CHX), 5h). The techniques of ultra-rapid freezing used in this study were essentially similar to those of described by Vajta et al (Theriogenology 1999; 52:939-948), Denuded oocytes at 22 h of culture were exposed to cryoprotectant (3.2 M Ethylene glycol, 2.36 M DMSO, 0.6 M sucrose), and followed by freezing in electron microscopic grid. After thawing the oocytes were transferred back into the drop of maturation medium and cultured for additional 2 h before being subjected to ICSI. All eggs were then cultured in CRlaa medium, and transferred into M199+10% FCS on day 4. The culture was maintained until day 9. In Experiment 1, frozen-ICSI eggs were compared on development into blastocyst to those of unfrozen and IVF control. Those eggs were activated with the method of group 2. A higher proportion of unfrozen-ICSI and IVF eggs developed into cleavage and blastocysts than of frozen-ICSI eggs (65% and 13%; 71% and 23% vs. 39% and 8%; P<0.05). In Experiment 2, development and ploidy of embryos made from group 1 were compared to those from group 2. Between groups there did not differ on the rates of development, however, chromosomal abnormality in group 1 was significantly higher than in group 2 (49% vs. 30%; P<0.05). The present result suggests that frozen bovine oocytes can be used for ICSI.

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