• 미생물학회지
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• 제1권1호
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• pp.19-25
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• 1963

The studies could be summarized as follows: 1. Standard Rf Values were made as Table II for the identification of amino acids. 2. Amounts of free amino nitrogen in eggs were increased about 0.03% (Table IV) while the amounts of total nitrogen (Table III) were increased lower percentage during the ten days of development. 3. Three unknown substances were found newly during the development. 4. Eight ninhydrine positive amino acids were present, in the fresh embryonic eggs, but the numbers increased to eighteen after fifty days of development. 5. Through the development, there were large amounts of glutamic acid, aspartic acid and alanine. 6. After fifty days development, the amount of amides, such as asparagine, glutamine and alanine were increased very large which were very small amount before it and the amount of asparagine were more than that of glutamine. 7. Tryptophane, Cystine , Histidine were detected after 50 days of development. This is very remarkable fact.

• 한국수산과학회지
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• 제49권5호
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• pp.628-634
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• 2016

We investigated the characteristics and rate of development of allotriploid embryos derived from a cross between female olive flounder Paralichthys olivaceus and male starry flounder Platichthys stellatus. The allotriploidy was induced by cold shocking fertilized eggs three minutes post-fertilization at 3°C for 45 minutes. The average cellular DNA content of the allotriploid embryos was 2.06±0.03 pg/cell, which is equal to the sum of the cellular DNA content of a diploid olive flounder (1.42 pg/cell) and a haploid starry flounder (0.66 pg/haploid cell). The first cleavage, midblastula, gastrula and Kupffer's vesicle appearance stages of the allotriploid eggs began at 1.5, 8, 13 and 26 hours after cold shocking at 18°C, respectively. The developmental rate of allotriploid eggs was equivalent to that of diploid and triploid olive flounder eggs at 10, 14 and 18°C. However, the hatching times of allotriploid eggs, 7 h at 10°C, 5 h at 14°C and 4 h at 18°C, were earlier than those of diploid and triploid olive flounder.

• 한국수산과학회지
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• 제46권6호
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• pp.957-965
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• 2013

This study simulated the egg vertical distribution of mackerel Scomber japonicus in Korean waters using general numerical models. All eggs were spawned naturally by raising broodfishes (May-June 2013), and the egg specific gravity was measured by a density-gradient column. CTD surveys provided environmental data (e.g., temperature and salinity) in May near Jeju Island, Korea. The egg specific gravity during the early stages ranged from 1.0203-1.0211. In general, the fertilized eggs showed a gradual decline in egg specific gravity until full development of the main organs, with a sudden increase just before hatching. Modeled egg vertical distributions were influenced more by wind speed than by egg buoyancy and vertical structure of the sea water. During calm and normal wind speeds, the eggs were distributed from the surface to 25-m depths. Under strong wind conditions (three times higher than the normal speed), the egg concentration on the surface decreased, and the egg distributional depth was deeper (~50 m).

• 한국수정란이식학회지
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• 제8권2호
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• pp.83-90
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• 1993

To investigate the effect of extracellular matrix proteins on the in vitro development of ethanol-induced parthenogenetic eggs of ICR strain mice, those were cultured in vitro in fibronectin, gelatin, or collagen precoated culture dishes containing 1.5 ml of NaH-C03$_3$-BMOC-3 medium at 37$^{\circ}C$ for 96 hrs. under the atmosphere of 5% $CO_2$ and 95% air. Fibronectin, gelatin, or collagen significantly(P$\pm$1.4, 45.4i1.4, and 44.8$\pm$O.9, respectively. And the diameter of those eggs ranged 104.6$\pm$1.9, 102.8$\pm$2.3, and 103.4$\pm$O.8 $\mu$m, respectively.

• 한국수산과학회지
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• 제50권6호
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• pp.829-836
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• 2017

This study investigated the species composition and abundance of floating fish eggs to determine the timing and location of spawning of fish inhabiting the coastal waters of Sagye, Jejudo Island. Eggs were collected with a Bongo net bimonthly from May 2009 to February 2010. Identifications were based on nucleotide sequences of the mitochondrial cytochrome c oxidase subunit I (COI) gene. Eggs were determined to belong to 43 distinct taxa, 35 of which were identified to the species level. The assemblage spanned eight orders, 23 families, and 32 genera. The number of taxa collected varied from month to month, with 14 taxa (12 species) found in June 2009, 11 taxa (10 species) in October 2009, 10 taxa (nine species) each in August 2009 and February 2010, eight taxa (six species) in April 2009, and five taxa (four species) in December 2009. Five abundant species (Branchiostegus japonicus, Engraulis japonicus, Pseudolabrus sieboldi, Goniistius zonatus, and Halichoeres tenuispinis) together represented 52.8% of the total number of eggs collected during the study.

• 한국발생생물학회지:발생과생식
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• 제24권1호
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• pp.19-30
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• 2020

The pH of water is one of the main environmental factors exerting selective pressure on marine and freshwater organisms. Here, we focus on the influence of pH on an organism's ability to maintain homeostasis and investigate the effects of acidification on immunity-related genes and osmotic pressure during early development of the olive flounder, Paralichthys olivaceus. The aim of our study was to determine the influence of various pH levels on the fertilized eggs and larvae of P. olivaceus. Gametes of P. olivaceus were artificially introduced and the resulting fertilized eggs were incubated at pH 4.0 (low), 6.0, and 8.0 (equivalent to natural sea water; control). We found that all eggs sank from the water column at pH 4.0. After 38 h, these eggs showed slow development. Hatching occurred more slowly at pH 4.0 and 6.0 and did not occur at all at pH 4.0. Result of gene expression, caspase and galectin-1 were expressed from the blastula to pre-hatch stages, with the exception of the two-cell stage. HSP 70 was also steadily expressed at all pH levels over the five days. The osmolality of fertilized eggs differed marginally at each stage and across pH levels. So, this results demonstrates that low pH level is detrimental to P. olivaceus fertilized eggs.

• 한국수산과학회지
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• 제21권3호
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• pp.139-144
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• 1988

1985년 $6\~8$월 1986년 $6\~9$월에 동해남부해역 냉수조사의 일환으로서 실시된 수온관측자료 및 개폐형채집망을 사용하여 층별로 채집된 멸치난과 자${\cdot}$치어를 분석하였다. 수온약층은 1985년의 경우 연안측에서는 표층가까이에서, 외해측에서는 $20\~50m$층에서 형성되었으나 1986년에는 외해측까지 계절수온약층의 경사가 완만하게 형성되었다. 또한 멸치날${\cdot}$치어의 분포역은 1985연에는 울산 부근의 외해측에서, 1986년에는 감포부근의 연안측에서 주로 분포하였다. 특히 멸치 난은 일출시에는 대부분 발생초기에 해당되며 $10\~30m$층에 주로 분포하였으나 자정무렵에는 발생후기로 이행되고 $50\~100m$층에서의 분포비율이 높아지므로서 멸치 난은 발생이 진행됨에 따라 침하하는 경향을 보였다. 또한 자${\cdot}$치어의 경우에는 야간에는$0\~30m$층에서 주간에는 30m이심에서의 분포비율이 높았다.

• 한국수산과학회지
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• 제36권5호
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• pp.486-494
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• 2003

Distribution of ichthyoplanktons and its relation to environmental factors (temperature, salinity, $chlorophyll\;\alpha$) around the Youngsan River Estuary of Mokpo, southwest coast of Korea were investigated based on the samples collected bimonthly from February to December, 2001. Unidentified fish eggs were most abundant $1,345\;eggs/100m^3$ in August, while eggs of Engraulis japonicus were most abundant $63\;eggs/100m^3$ in June. A total of forty species of fish larvae and juveniles belonging to the twenty families under six orders were caught, with six gobiids, four icefishes, four croakers and three herrings being represented. Fish larvae and juveniles peaked $17,757\;inds./100m^3$ in August, which consisted of $91.02\%$ of gobiids, $3.69\%$ of gunnels and $3.67\%$ of icefishes, and they were most abundant $7,467\;Inds./100m^3$ at St. 7. Cluster analysis based on the individuals of fish larvae and juveniles showed that six months were classified into two groups at the dissimilarity 70, the first was composed of June and August and the second was February and December and April and October were not clustered with any other months. In August when species diversity index was the highest, eight stations were classified into two groups at the dissimilarity 70, the first was composed of St. 1, 3, 4, 5 and 8 and the second was St. 6, 7 and 2. As a result from correspondence analysis, there is no effect of the environmental factors with a density of fish eggs and larvae, but correlation could be found between a density of fish eggs and $chlorophyll\;\alpha.$

• 한국잠사곤충학회지
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• 제40권1호
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• pp.23-26
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• 1998

Exposure of freshly laid eggs of Exorista bombycis (Louis) to volatiles emanating from bulbs of Allium sativum L. for different durations resulted in signigicant reduction in their hatchability. Maggots hatched from the eggs exposured for 64 h and 72 h were failed to emerge from host larvae. The duration of development stages of E. bombycis was prolonged besides reduction in rate of pupation and adult emergence as the egg exposure period increased. The findings are interpreted as the chronic effects of volatiles of garlic affecting maggots following developmental defects sustainable during embryonic development. The known major chemical components of A. sativum such as allicin, thioacrolein, ajoune, 2-propene sulfenic acid, 2-propene thiol and propylene were presumed to be responsible for the adverse consequences reported in this paper.

• 한국가금학회지
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• 제26권1호
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• pp.43-50
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• 1999

The features of special brand eggs are growing more and more diverse and it is hard to draw a clear distinction between these and ordinary (no-brand) eggs. Special brand eggs range from those with objectively recognizable characteristics to those given only an unsubstantial product image and price differentials are among them great, too. The relation between product features and prices is unclear. Special brand eggs are the commodity whose characteristics are the vaguest of all livestock products. Farm's brand eggs produce a high profitability to producers is they are directly sold to consumers, by, for example, home-delivery service. But if they are sold to supermarkets, etc., producer's (poultry farmer's) income becomes lower by the amount of distributors' margin, reducing the profitability substantially. Thus how to increase the ration of retailing is important for farmers to secure a high profit. The sales strategies of poultry are the combination of two elements, that is, new product development (product differentiation) and creation of new market. But it is difficult for special brand egg producers to develop products with clear characteristics (use value) distinct form ordinary eggs and so these producers depend on the factors of appearance, such as the color of egg shells and package. Special brand eggs manage to keep their marketable value by the combination of the few product features and product image. Thus NB eggs from feed producers have a great market-ability since they can take advantage of the power of patents and TV commercials. However, market differentiation affects profitability much more than product features and price gaps are very wide between directly sold and wholesaled eggs. The producers of special brand eggs have come to the turning point where they have to decide whether they will content with being the subcontractors for NB and PB eggs or they will continue to keep their independence in production and marketing.