• Asian-Australasian Journal of Animal Sciences
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• 제18권4호
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• pp.545-551
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• 2005

A feeding trial was conducted to determine whether dietary vitamin C (200 mg/kg) and vitamin E (250 mg/kg) prevent any drops in egg shell quality under heat stress in broiler breeder hens. One hundred and sixty molted Ross broiler breeders were housed randomly in an individual cage at 83 weeks of age. Four dietary treatments with forty hens and four replications per treatment were control (no additional vitamins), vitamin C-, or vitamin E-supplemented and combined supplementation of the two vitamins. After a tenday-adaptation period at 25$^{\circ}C$, the ambient temperature was kept at 32$^{\circ}C$ for a three-week-testing period. Egg production dropped dramatically over week but it did not show a significant change among treatments (p<0.05). However, egg quality parameters such as egg weight, specific gravity, shell thickness, SWUSA, puncture force and shell breaking strength from the birds fed the diet with the combined vitamins C and E were significantly improved over those of the control group during the heat stress period (p<0.05). The hens fed the vitamin C diet improved tibia breaking strength (37.16 kg), statistically higher than the birds fed the control and the vitamin E diets (p<0.05). The hens fed the control diet showed higher serum corticosterone levels, a mean of 5.97 ng/ml, than those of the other treatments (p<0.05). The heat stress resulted in elevated heterophils and decreased lymphocytes in serum, increasing the H/L ratios for all the treatments. However, the increases in H/L ratios were alleviated by feeding the diets containing vitamin C alone or together with vitamin E, although there were no significant differences in the ratio between the two groups (p<0.05). In conclusion, vitamins C (200 mg/kg) and/or E (250 mg/kg) supplemented to the diets for broiler breeder hens could prevent drops in egg shell quality and tibia bone strength under highly stressful environmental temperatures.

• 한국가금학회지
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• 제48권4호
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• pp.319-325
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• 2021

In this study, we investigated the effects of feeding diets with different levels of energy and nutrient density on the egg quality of laying hens during the pre-peak and peak periods. A total of 192 (Hy-line brown) laying hens were used in a 15-week trial. The hens were randomly allotted to one of four treatments, each with four replicates (12 hens per replication). We assessed the effects of four level of dietary energy (2,710, 2,850, 2,870 and 2,890 kcal/kg) and three levels of nutrient density (Methionine + Cysteine: 0.56%, 0.85%, 0.80% and Crude Protein: 14.5%, 19%, 18%). Differences in the energy and nutrient density contents of diets showed no significant effect (P>0.05) on the average daily gain, average daily feed intake, feed conversion ratio, egg weight, or egg production of hens during the pre-peak and peak periods. However, hens subjected to 2,890 kcal/kg during the pre-peak period were found to lay eggs with significantly thicker shells, and yolk color was found to be significant enhanced in hens fed this diet during the pre-and peak periods. In contrast, we detected no significant effects of dietary energy or nutrient density on the Haugh unit or eggshell strength. In summary, increasing the energy level of diets from 2,710 to 2,890 kcal/kg was found to have positive effects on the shell thickness and yolk color of eggs produced by laying hens.

• Journal of Animal Science and Technology
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• 제65권2호
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• pp.351-364
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• 2023

The experiment was carried out to study the effect of Korean wild ginseng adventitious root supplementation on the laying performance, egg quality, cytokine expression, ginsenoside concentration, and microflora quantity of Institut de selection Animale (ISA) brown laying hens at 24 weeks old. A total of 90 laying hens were subjected to a completely randomized design at three treatments, five repetitions and six laying hens per replicate. The experiments were divided by diets into the basic feed (CON), basic feed + 0.1% wild ginseng (WG1), and basic feed + 0.5% wild ginseng (WG2). The feeding trial was carried out over a duration of 12 weeks after an initial acclimation period of 2 weeks. Feeds and water were administered ad libitum in mash form, and light was available for 16 hours per day. At the end of study, henday egg production (HDEP), average egg weight (AEW), and egg mass (EM) were increased (p <0.05) in WG2 at week 12. Feed conversion ratio (FCR) was decreased (p < 0.05) in WG2 at week 12. The ginsenoside content in egg yolk was increased (p <0.05) in laying hens in the WG2 treatment at week 12. Relative expression of tumor necrosis factor alpha (TNF-α) was reduced (p < 0.05) in the WG supplemented diets at week 12. The fecal microflora quantity of Lactobacillus was increased (p < 0.05) in WG2 at week 8 to week 12, and Escherichia coli (E. coli) was significantly decreased (p < 0.05) in the WG2 at week 12. We concluded that the result observed in the HDEP, AEW, EM and FCR was due to an increase in ginsenoside content, leading to an improvement in the TNF-α, and fecal microflora quantity such as Lactobacillus and E. coli in the WG2 supplemented diets. We therefore recommend the use of WG at application level 0.5% per basal diet for optimum laying performance in layer hens.

• Animal Bioscience
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• 제37권8호
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• pp.1452-1462
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• 2024

Objective: This study aimed to investigate the effects of dietary supplementation with Agaricus blazei polysaccharide (ABP) at varying concentrations on the performance, egg quality, blood biochemistry, intestinal morphology, and microflora of quail. Methods: The study involved a total of 2,700 Korean quails, which were randomly divided into three groups. The measured variables encompassed productive performance, egg parameters, carcass parameters, serum metabolites, immune response parameters, antioxidative properties, and gut microbiome. Results: The addition of ABP did not have a significant effect on average daily feed intake. However, it was found to increase the average daily egg weight and egg production rate, reduce the feed-egg ratio. There were no significant impacts on egg quality measures such as egg shape index, egg yolk index and color, egg yolk and protein content. However, ABP supplementation significantly increased the Hough unit (p<0.01) and decreased the rate of unqualified eggs (p<0.01). Regarding serum parameters, the inclusion led to an increase in total protein concentration (p<0.05) and a reduction in low-density lipoprotein cholesterol (p<0.05). There were no significant effects observed on immune indicators such as immunoglobulin A (IgA) and IgM. ABP supplementation increased the levels of serum antioxidant indicators, including glutathione peroxidase, total superoxide dismutase (p<0.05), and total antioxidant capacity colorimeter (p<0.05). Furthermore, ABP supplementation significantly elevated the intramuscular fatty acid content in quail meat. Additionally, ABP supplementation demonstrated a significant improvement in the diversity of gut microbiota and induced alterations in the composition of the gut microbiota. Conclusion: The findings of this study indicate that dietary supplementation of ABP enhanced production performance and antioxidant capacity while increasing the levels of polyunsaturated fatty acids in quail muscle.

• 한국가금학회지
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• 제50권4호
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• pp.213-229
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• 2023

본 연구는 한국 토종닭을 대상으로 부리 다듬기 여부와 교배조합이 생산능력 및 스트레스 반응 정도에 미치는 영향을 살펴 보고자 한 것이다. 시험은 6개의 토종닭 교배조합 248수를 대상으로 부리 다듬기를 실시한 그룹과 하지 않은 그룹으로 나누고, 이들의 생존율, 체중, 산란율, 난질, 깃털 손상도, HSP-70 유전자 발현량, H/L ratio 및 세포 내 DNA 손상률을 조사하여 비교 분석하였다. 분석 결과, 부리 다듬기를 한 그룹이 하지 않은 그룹보다 생존율 및 산란지수는 유의하게 높았고, 깃털 손상도 및 DNA 손상률은 유의하게 낮았다(P<0.05). 반면 육성기 이후 체중, 일계산란율, 난질, HSP-70 유전자 발현율 및 H/L ratio는 부리 다듬기 여부에 따른 차이가 없는 것으로 나타났다. 한편, 교배조합 간에는 생존율, 체중, 깃털 손상도, 난질 및 DNA 손상률에 유의한 차이를 보이고(P<0.05), 산란율, HSP-70 유전자 발현율 및 H/L ratio의 차이는 없는 것으로 나타났다. 생존율, 깃털 손상도 및 DNA 손상률은 부리 다듬기와 교배조합 간의 상호작용이 있는 것으로 나타났다. 이상의 분석 결과로부터 토종닭의 부리 다듬기가 생산능력에 긍정적인 영향을 미치고, 스트레스 반응 정도에 있어서 육성기 이후부터는 부리 다듬기가 스트레스 요인으로 작용하지 않거나, 오히려 스트레스감소 요인으로 작용하는 것으로 판단된다. 또한 토종닭 교배조합 간에 생산능력 및 스트레스 반응 정도의 차이는 있으나 조합별 부리 다듬기가 이들에 미치는 영향은 거의 비슷한 것으로 보인다.

• Asian-Australasian Journal of Animal Sciences
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• 제22권11호
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• pp.1587-1593
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• 2009

Duck egg white (DEW) hydrolysates were prepared by five enzymes (papain, trypsin, chymotrypsin, alcalase, and flavourzyme) and their antioxidant activities investigated in this study. DEW hydrolyzed with papain (DEWHP) had the highest peptide content among the five enzymatic treatments. Besides, the peptide content of DEWHP increased when the enzyme to substrate ratio (E/S ratio) increased. It was suggested that higher E/S ratio contributed to elevate the degree of hydrolysis of DEW effectively. Similar results were also obtained by Tricine-SDS-PAGE. In addition, SDS-PAGE patterns indicated papain was the only one amongst all enzymes with the ability to hydrolyze DEW. In antioxidant properties, DEWHP showed more than 70% of inhibitory activity on linoleic acid peroxidation and superoxide anion scavenging. Moreover, the $Fe^{2+}$ chelating effect of DEWHP was greater than 90%, while no significant difference was observed in DPPH radical scavenging and reducing ability. The results of peptide contents, antioxidant activities and electrophoresis suggested that the higher the peptide content, the stronger the antioxidant activities in DEWHP.

• Asian-Australasian Journal of Animal Sciences
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• 제14권6호
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• pp.831-836
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• 2001

Little information on the cholesterol content and the fatty acid composition of avian species other than chicken is available. This study was conducted to compare the yolk cholesterol content and the fatty acid profiles of some wild birds maintained in captivity on commercial grain-based chicken diets. The concentration of cholesterol/g of yolk as well as the total yolk cholesterol per egg varied among species. Yolk cholesterol concentration, expressed as mg/g of yolk, was highest in chukar, followed by pheasant, guinea fowl and quail, while total yolk cholesterol in an egg was highest in guinea fowl, followed by pheasant, chuckar and quail. An inverse relationship between yolk cholesterol concentration and egg weight was observed among species with an exception of quail. Although major fatty acids of egg yolk were oleic acid, palmitic acid, linoleic acid and stearic acid in all birds, the composition varied among species. Chukar and quail showed higher oleic acid content than pheasant and guinea fowl, while showing lower linoleic acid. Fatty acids of chukar and guinea fowl eggs were more saturated than those of pheasant and quail. Chukar and especially quail had higher monounsaturated fatty acids (MUFA) than pheasant and guinea fowl; in quail egg 51.6% of total fatty acids were MUFA. Polyunsaturated fatty acids (PUFA), essential fatty acids (EFA) and the ratio of PUFA to saturated fatty acid (P/S ratio) were higher in pheasant and guinea fowl than in chukar and quail. Differences in fatty acid profile of triglyceride (TG) among birds were largely similar to those of total lipid. In comparison to TG, phosphatidyl choline (PC) was low in MUFA while high in saturated fatty acids (SFA), PUFA, P/S ratio and EFA. PC was most saturated in guinea fowl egg yolk, followed by chukar, quail and pheasant. PUFA, P/S ratio and EFA in PC were highest in pheasant followed by chukar, guinea fowl and quail. PE was distinguished from PC by its high contents of stearic acid, eicosapentenoic acid (EPA) and docosahexenoic acid (DHA) while low in palmitic, oleic and linoleic acids. In egg yolk of all birds MUFA was significantly lower in PE than in PC except in quail. Compared to other species, quail had a considerably higher content of MUFA in PE at the expense of SFA and PUFA.

• 한국응용과학기술학회지
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• 제34권3호
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• pp.577-586
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• 2017

본 연구의 목표는 분변토로부터 분리한 Bacillus subtilis외 2종의 복합 생균제를 이용하여 제조한 발효사료가 산란계 생산성, 계란품질 및 양계장 악취저감에 미치는 효과를 조사하는 것이었다. 산란계 240마리를 4개의 처리구로 구분하여 완전임의 배치하였다. 실험 처리구는 대조구, 분변토발효사료 0.3, 0.5% (FECF3, FECF5), 일반생균제 0.2% (CP)로 구분하였다. 산란율은 일반사료를 섭취한 대조구와 비교할 때 FECF 처리구는 유의하게 높았으나 CP 처리구는 차이가 없었다. 호우유닛, 난각두께, 파란강도, 난황색 지수 및 농후난백 높이는 대조구와 비교할 때 FECF 처리구가 증가하였으나 CP 처리구와 차이는 없었다. 계란의 n-3 함량은 대조구와 비교할 때 FECF 처리구가 높았으나 n-6/n-3 비율은 낮았으며 CP 처리구와의 차이는 없었다. 계분을 실온에서 5일간 방치 후 암모니아 발생량은 대조구와 비교할 때 CP와 FECF 처리구가 낮았다.

• Asian-Australasian Journal of Animal Sciences
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• 제33권2호
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• pp.313-322
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• 2020

Objective: An experiment was conducted to investigate the response of laying hens fed corn distiller's dried grains with solubles (DDGS) that are naturally contaminated with deoxynivalenol (DON). Methods: One hundred and sixty 52-week-old Lohmann Brown Lite hens were randomly allotted to five dietary treatments with 8 replicates per treatment. The dietary treatments were formulated to provide a range of corn DDGS contaminated with DON from 0% to 20% (i.e., 5% scale of increment). All laying hens were subjected to the same management practices in a controlled environment. Body weight, feed intake and egg production were measured biweekly for the entire 8-week experiment. The egg quality was measured biweekly for 8 weeks. On weeks 4 and 8, visceral organ weights, blood metabolites, intestinal morphology, and blood cytokine concentrations were measured. Results: The inclusion of corn DDGS contaminated with DON in the diet did not alter (p>0.05) the body weight, feed intake, hen-day egg production, egg mass and feed efficiency of the laying hens. No difference was found (p>0.05) in the egg quality of hens that were fed the dietary treatments. Furthermore, hens that were fed a diet containing corn DDGS contaminated with DON showed no change (p>0.05) in the visceral organ weights, the blood metabolites, and the cytokine concentrations. The crypt depth increased (p<0.05) as the amount of corn DDGS contaminated with DON increased. Proportionately, the villus height to crypt depth ratio of the laying hens decreased (p<0.05) with the increasing level of corn DDGS contaminated with DON in the diet. Conclusion: The inclusion of corn DDGS contaminated with DON up to 20% in layer diets did not cause changes in egg production performance and egg quality, which indicates that DON is less toxic at the concentration of 1.00 mg DON/kg.

• 한국식품영양학회지
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• 제30권2호
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• pp.269-281
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• 2017

In this study, quality characteristics and antioxidative activities were comparatively evaluated between the macaron with egg white powder added at the ratios of 2, 4, 6 and 8% and the macaron with added liquid egg white, in order to determine the applicability of the macaron with added egg white powder. The analysis of amino acids indicated that the group with 8% of egg white powder added showed the highest total amino acid content of 7,584.20 mg/100 g. The group with 4% of egg white powder added showed the highest sweetness (p<0.01); whereas, and the group with 2% of egg white powder added showed the highest pH (p<0.001). In terms of the chromaticity, L (p<0.01) and a values (p<0.05) were increased with the increasing percentage of added egg white powder. With the increasing percentage of added egg white powder, textures such as hardness (p<0.01), chewiness (p<0.05), gumminess (p<0.001), and cohesiveness (p<0.001) decreased; whereas, adhesiveness (p<0.001) and springiness (p<0.01) increased. The results of the sensory evaluation indicated the highest preference for the macaron containing 6% white egg powder, in all items. DPPH free radical scavenging activity, SOD-like activity, and reducing power were increased with increasing amounts of added egg white powder (p<0.001). Collectively, the results indicated that the production of macaron with the addition of 6% of egg white powder is the most suitable mixing ratio for enhancing the preference and quality level.