• Bulletin of the Korean Chemical Society
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• 제5권4호
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• pp.154-158
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• 1984

In order to characterize the permeability of small unilamellar vesicles (SUV), efflux of 6-carboxyfluorescein (6-CF) from the vesicles was monitored spectrophotofluorometrically. Since the entrapped highly quenched 6-CF (200 mM) became fluorescent upon release from the vesicles, the 6-CF could be used as an efflux probe. SUV containing entrapped 6-CF was prepared from egg phosphatidylcholine and separated by gel filtration on Sepharose 4B. Observed change of relative fluorescent intensity with time was sigmoidal. From this curve, the parameter of permeability was determined either by half-time or a released amount per unit time from the initial slope. Half-time of efflux of prepared SUV having 302 ng phospholipid/ml in 10 mM Tris-HCl buffer pH 7.4 was 21.0 min at $37{\circ}C$. Various factors which could affect the half-time were examined including temperature, pH, salt, and vesicle concentration. In particular the effect of vesicle concentration on the efflux revealed that the permeability can be a function of the concentration.

• Journal of Ecology and Environment
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• 제32권2호
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• pp.67-73
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• 2009

Soil $CO_2$ efflux can vary markedly in magnitude over both time and space, and understanding this variation is crucial for the correct measurement of $CO_2$ efflux in ecological studies. Although considerable research has quantified temporal variability in this flux, comparatively little effort has focused on its spatial variability. To account for spatial heterogeneity, we must be able to determine the number of sampling points required to adequately estimate soil $CO_2$ efflux in a target ecosystem. In this paper, we report the results of a study of the number of sampling points required for estimating soil $CO_2$ efflux using a closed-dynamic chamber in young and old Japanese cedar plantations in central Japan. The spatial heterogeneity in soil $CO_2$ efflux was significantly higher in the mature plantation than in the young stand. In the young plantation, 95% of samples of 9 randomly-chosen flux measurements from a population of 16 measurements made using 72-$cm^2$ chambers produced flux estimates within 20% of the full-population mean. In the mature plantation, 20 sampling points are required to achieve means within $\pm$ 20% of the full-population mean (15 measurements) for 95% of the sample dates. Variation in soil temperature and moisture could not explain the observed spatial variation in soil $CO_2$ efflux, even though both parameters are a good predictor of temporal variation in $CO_2$ efflux. Our results and those of previous studies suggest that, on average, approximately 46 sampling points are required to estimate the mean and variance of soil $CO_2$ flux in temperate and boreal forests to a precision of $\pm$ 10% at the 95% confidence level, and 12 points are required to achieve a precision of $\pm$ 20%.

• 한국산림과학회지
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• 제98권2호
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• pp.183-188
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• 2009

It is necessary to determine the amount of carbon dioxide ($CO_2$) absorbed by plants and released from forest floor into atmosphere, to gain a better understanding how forests participate in the global carbon cycle. Soil $CO_2$ efflux, litter production, and decomposition were investigated in Q. variabilis and P. densiflora stands in the vicinity of Gwangju, Chonnam province. Soil $CO_2$ efflux was measured using Infrared Gas Analyzer (IRGA) at midday of the 10th day at every month over 12-month period, to quantify seasonal and annual budgets of soil $CO_2$ efflux. Soil temperature and soil moisture were measured at the same time. Seasonal soil $CO_2$ efflux in Q. variabilis and P. densiflora were the highest in summer season. In August, maximum soil $CO_2$ efflux in Q. variabilis and P. densiflora was 7.49, $4.61CO_2{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, respectively. Annual $CO_2$ efflux in each stand was 1.77, $1.67CO_2kg{\cdot}m^{-2}$ respectively. Soil $CO_2$ efflux increased exponentially with soil temperature and related strongly in Q. variabilis ($r^2$=0.96), and in P. densiflora ($r^2$=0.91). Litter production continued throughout the year, but showed a peak on November and December. Annual litter production in the Q. variabilis and P. densiflora stands were $613.7gdw{\cdot}m^{-2}{\cdot}yr^{-1}$ and $550.5gdw{\cdot}m^{-2}{\cdot}yr^{-1}$.$yr^{-1}$, respectively. After 1 year, % remaining mass of Q. variabilis and P. densiflora litter was 48.2, 57.1%, respectively. The soil $CO_2$ efflux rates in this study showed clear seasonal variations. In addition, the temporal variation in the $CO_2$ efflux rates was closely related to the soil temperature fluctuation rather than to variations in the soil moisture content. The range of fluctuation of soil $CO_2$ efflux and litter decomposition rate showed similar seasonal changes. The range of fluctuation of soil $CO_2$ efflux and litter decomposition rate was higher during summer and autumn than spring and winter.

• Journal of Pharmaceutical Investigation
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• 제38권5호
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• pp.295-302
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• 2008

In the present study, we examined the changes of uptake and efflux of taurine under various conditions inducing oxidative stress using rat conditionally immortalized syncytiotrophoblast cell line, TR-TBT cell, as blood-placental barrier in vitro model. In addition, we identified the characteristics of taurine transport in TR-TBT cells including general features, besides effect of calcium ion on taurine transport. Taurine uptake showed time, $Na^+$ and $Cl^-$ dependency, and was decreased by PKC activator in TR-TBT cells. Also, calcium free condition decreased taurine uptake and evoked taurine efflux in the cells. Oxidative stress induced the change of taurine transport in TR-TBT cells, but the changes were different depending on the types of stimulation inducing oxidative stress. The taurine uptake was increased by TNF-$\alpha$, LPS and DEM stimulation but decreased by $H_2O_2$ and NO stimulation. Also, the taurine efflux was regulated by TNF-$\alpha$ stimulation. In conclusion, the taurine transport through the blood-placental barrier was regulated in oxidative stress conditions, and these results demonstrated that oxidative stress affected the taurine supplies to fetus and taurine level of fetus.

• Ecology and Resilient Infrastructure
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• 제8권1호
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• pp.32-43
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• 2021

삼림 생태계에서 토양 CO2 유출량의 연속적인 모니터링을 위한 다중채널 자동챔버시스템 (MCACs)을 개발하였다. MCACs는 자동개폐 덮개가 있는 8개의 토양챔버, 8개의 다중채널 가스샘플러를 장착한 적외선 CO2 농도 분석기, 시간 릴레이 회로를 갖춘 전자 컨트롤러, 프로그래밍이 가능한 로직용 자료수집장치로 구성되었다. 사계절 장기간에 걸쳐 야외 현장에서 MCACs의 안전성과 신뢰도를 조사하고, 높은 시간분해능으로 현장 테스트에서 얻은 온도와 토양수분 함량이 토양 CO2 유출에 미치는 효과들을 파악하기 위해, 2010년 1월부터 12월 사이의 기간 동안 남산의 신갈나무림 실험지소에서 MCACs를 사용하여 토양 CO2 유출속도와 미기상 요인들을 지속적으로 측정하였다. 토양 CO2 유출속도의 일변화 및 계절적인 변화는 현저하게 온도 요인의 경향을 따랐다. 전체 실험 기간 동안에 토양 CO2 유출속도는 5 cm 깊이의 토양온도와 밀접한 상관관계 (r2 = 0.92)를 보였으나 토양수분 함량과는 약한 상관관계 (r2 = 0.27)를 나타냈다. 남산 신갈나무림에서 온도에 대한 토양 CO2 유출량의 연간 민감도(Q10 수치)는 2.23~3.0의 범위에 달했고, 다양한 온대지역의 낙엽활엽수림에 대한 다른 연구들과 일치하였다. MCACs에 의해 측정된 연평균 토양 CO2 유출량은 약 11.1 g CO2 m-2 day-1이었다. 이 결과들은 MCACs가 야외 현장에서 토양 CO2 유출량의 연속적인 장기측정과 동시에 미기상 요인들의 영향을 결정하는데 유용한 시스템임을 나타낸다.

• 콘크리트학회논문집
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• 제12권5호
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• pp.111-120
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• 2000

In case of constructing concrete structure under water, generally concrete mixed with antiwashout admixture, high range water reducer, or AE-water reducing agent etc has been manufactured to ensure the quality of antiwashout underwater concrete because of being difficulty in ascertaining construction situation by the naked eye. The properties of high quality antiwashout underwater concrete that were aimed at affluent fluidity, workability and the compressive strength of 450 kgf/$\textrm{cm}^2$ at 28 ages using two types of blended cements are following as;(1) Setting time of antiwashout underwater concretes using blended cements was more greatly delayed than that of control concrete, however, was satisfied with criteria value of "Quality standard specification of antiwashout admixture for concrete".(2) As a test results of slump flow, efflux time and box elevation of head, it was found that workability of high quality antiwashout underwater concrete was improved. (3) Heat evolution amount of OPC was 1.5 times as high as that of two types of bended cements in 72 hours. (4) Suspended solids of antiwashout underwater concrete using blended cements was more than that of control concrete, also compressive strength of high quality antiwashout underwater concrete was very low in early age, but was better than that of control concrete as to increasing ages.

• The Korean Journal of Physiology
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• 제8권1호
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• pp.45-53
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• 1974

The effects of kanendomycin on the potassium permeability in the rabbit erythrocyte membrane are investigated and the results are summarized as follows. 1. Kanendomycin causes the efflux of $K^+\;and\;influx\;of\;Na^+$ across the rabbit erythrocyte membrane. 2. Osmotic resistance of kanendomycin treated erythrocytes is diminished. This diminution of osmotic resistance is more pronounced by increasing concentration of kanendomycin and longer incubation time. But osmotic resistance is rather increased in the presence of lower concentration of kanendomycin. 3. Cysteine and glutathione have little influence on $K^+$ efflux induced by kanendomycin. 4. EDTA has no effect on the increase in $K^+$ outflux by kanendomycin while PCMB augments slightly on $K^+$ outflux. 5. Kanendomycin inhibits $Ca^{++}$ binding competitively to rabbit erythrocyte membrane fragments.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2003년도 가을 학술발표회 논문집
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• pp.457-460
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• 2003

This paper discusses laboratory evaluations to assess the physical properties of antiwashout underwater concrete (AWC) containing pozzolanic materials such as fly ash (FA), blast furnace slag (SG) and metakaolin(MK). For the experiments, pH value, suspended solids, slump flow, efflux time and compressive strength were tested. According to the results from compressive strength test, MK10 showed the very high compressive strength characteristic during the entire curing days, but the rate of strength development was decreased as time goes by.

• Asian Pacific Journal of Cancer Prevention
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• 제13권9호
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• pp.4807-4814
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• 2012

Purpose: The chemoresistance of human hepatocellular carcinoma (HCC) to cytotoxic drugs, especially intrinsic or acquired multidrug resistance (MDR), still remains a major challenge in the management of HCC. In the present study, possible mechanisms involved in MDR of HCC were identified using a 5-fluorouracil (5-FU)-resistant human HCC cell line. Methods: BEL-7402/5-FU cells were established through continuous culturing parental BEL-7402 cells, imitating the pattern of chemotherapy clinically. Growth curves and chemosensitivity to cytotoxic drugs were determined by MTT assay. Doubling times, colony formation and adherence rates were calculated after cell counting. Morphological alteration, karyotype morphology, and untrastructure were assessed under optical and electron microscopes. The distribution in the cell cycle and drug efflux pump activity were measured by flow cytometry. Furthermore, expression of potential genes involved in MDR of BEL-7402/5-FU cells were detected by immunocytochemistry. Results: Compared to its parental cells, BEL-7402/5-FU cells had a prolonged doubling time, a lower mitotic index, colony efficiency and adhesive ability, and a decreased drug efflux pump activity. The resistant cells tended to grow in clusters and apparent changes of ultrastructures occurred. BEL-7402/5-FU cells presented with an increased proportion in S and G2/M phases with a concomitant decrease in G0/G1 phase. The MDR phenotype of BEL-7402/5-FU might be partly attributed to increased drug efflux pump activity via multidrug resistance protein 1 (MRP1), overexpression of thymidylate synthase (TS), resistance to apoptosis by augmentation of the Bcl-xl/Bax ratio, and intracellular adhesion medicated by E-cadherin (E-cad). P-glycoprotein (P-gp) might play a limited role in the MDR of BEL-7402/5-FU. Conclusion: Increased activity or expression of MRP1, Bcl-xl, TS, and E-cad appear to be involved in the MDR mechanism of BEL-7402/5-FU.

• 대한의생명과학회지
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• 제20권4호
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• pp.237-243
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• 2014

Triglyceride (TG) can cause death of macrophages and formation of foam cells thereby increasing inflammation in atherosclerotic plaques. Accumulation of cholesterol in macrophages is another critical event that promotes development of inflammatory cardiovascular diseases. Several proteins are known to transport intracellular cholesterol outside of the cell and these proteins are thought to be protective against atherosclerosis pathogenesis. It is unknown whether TG can affect cholesterol efflux in macrophages. In the current study, we examined mRNA expression levels of genes that promote efflux of cholesterol (ABCA1, ABCG1 and SR-B1). We found that TG treated THP-1 macrophages exhibited an increase in ABCG1 expression in a dose- and time-dependent manner. In contrast, the expression of ABCA1 and SR-B1 remained unchanged. To identify cell signaling pathways that participate in up-regulation of ABCG1, THP-1 macrophages were treated with various cell signaling inhibitors. We found that inhibition of the JNK and p38 MAPK pathway completely abrogated up-regulation of ABCG1 whereas inhibition of MEK1 further enhanced ABCG1 expression in TG treated THP-1 macrophages. Also, TG induced phosphorylation of JNK and p38 MAPK in THP-1 macrophages. These results suggest that TG may potentially influence cholesterol efflux in macrophages.