• 산업기술연구
• /
• 제24권B호
• /
• pp.139-142
• /
• 2004

To identify conserved structural or functional subunit(s) in the CP1 (editing) domains of class Ia tRNA synthetases, five available structures were compared and analyzed. Through sequence alignments of the CP1 domains, three conserved regions were found near the amino acid binding site in the editing domain. Structural overlapping of the three subunits clearly showed that there exist three common structural subunits in all of the five editing RS structures. The new alignment suggests a translocation movement of the CP1 domain caused by the binding with tRNA. Based on the experimental and modeling results, it is proposed that subunits 1 and 3 accommodate the incoming amino acid binding, while subunit 2 contributes to the interactions with the adenosine ring of the A76 to stabilize the overall tRNA binding.. Since these subunits are critical for the editing reaction, we expect that these key structures should be conserved through all class Ia editing RSs.

• Bulletin of the Korean Chemical Society
• /
• 제28권2호
• /
• pp.207-210
• /
• 2007

To identify structural or functional common subunit(s) in the CP1 (editing) domains of class Ia tRNA synthetases, five available structures were compared and analyzed. Through the sequence alignments and structural overlapping of the CP1 domains, three conserved regions were identified near the amino acid binding site in the editing domain. Structural overlapping of the three subunits clearly showed the existence of three common structural subunits in all of the five editing RS structures. Based on the established experimental results and our modeling results, it is proposed that subunits 1 and 3 accommodate the incoming amino acid binding, while subunit 2 contributes to the interactions with the adenosine ring of the A76 to stabilize the overall tRNA binding. Since these subunits are critical for the editing reaction, we expect that these key structures should be conserved through the most class Ia editing RSs.

• 방송공학회논문지
• /
• 제7권4호
• /
• pp.355-361
• /
• 2002

본 논문은 MPEG-2 시스템 스트림 영역에서의 접합(splicing)이 MPEG-2 비디오 화면의 손상 없는 재생을 보장할 수 있는 방법을 연구한다. 기존의 접합은 MPEG-2 시스템의 TS 영역에서 수행하고 있는데, 비디오 문제로 한정시킨 영상편집의 관점에서 보면, 화면의 깨짐이 없는 임의의 픽처에서의 접합을 보장할 수 없다. 본 논문에서는 MPEG-2 시스템의 PES 영역에서의 비디오 화면을 고려한 접합을 다룸으로서 상위의 TS와 PS에서 동일한 목적의 접합을 수행할 수 있도록 하는 연구의 기반을 마련하고자 하였다. 그러기 위해서 먼저, 자각 다른 두 PES 스트림의 접합 시 발생하는 문제와 이로 인해 비디오 화면에 미치게 되는 영향을 비교, 분석하였고. 이를 토대로, PES 영역에서 직접 비디오 화면의 재생시에 일어날 수 있는 문제의 원인을 해결하는 방안을 모색하였다. 그 결과, PES 패킷의 헤더 정보 중 PES 패킷 길이 필드 값을 이용하면, PES 패킷의 상태와 관계없이 GOP 단위의 접합을 보장할 수 있음을 확인하였다.

• BMB Reports
• /
• 제49권9호
• /
• pp.497-501
• /
• 2016

Wide-line ¹H NMR intensity and differential scanning calorimetry measurements were carried out on the intrinsically disordered 73-residue full transactivation domain (TAD) of the p53 tumor suppressor protein and two peptides: one a wild type p53 TAD peptide with a helix pre-structuring property, and a mutant peptide with a disabled helix-forming propensity. Measurements were carried out in order to characterize their water and ion binding characteristics. By quantifying the number of hydrate water molecules, we provide a microscopic description for the interactions of water with a wild-type p53 TAD and two p53 TAD peptides. The results provide direct evidence that intrinsically disordered proteins (IDPs) and a less structured peptide not only have a higher hydration capacity than globular proteins, but are also able to bind a larger amount of charged solute ions.

• 한국정보과학회논문지:정보통신
• /
• 제27권2호
• /
• pp.109-122
• /
• 2000

MPEG 형식의 비디오 데이타의 사용이 점차 늘어남에 따라, 이런 데이타를 자유롭게 편집할 수 있는 편집기의 필요성이 점차 증가하고 있다. 그러나 MPEG 데이타는 연속된 프레임들의 차이만을 저장하는 방법을 사용하기 때문에 두 개의 MPEG 데이타를 전환 효과를 주어서 편집을 하기 위해서는 압축을 해제한 후 효과를 주고 다시 압축하여야 하는 문제점을 가지고 있다. 이런 문제점을 해결하기 위하여 본 논문에서는 MPEG 형식으로 압축된 두 비디오 데이타에 대하여 부분적으로 압축을 해제한 상태에서 여러 종류의 장면 전환 효과(페이드 인 및 페이드 아웃, 디절브)를 적용할 수 있는 방법을 제안하였다. 제안한 방법에서는 MPEG형식 비디오 데이타의 I와 P프레임에 대하여서는 압축을 부분적으로 해제하는 전환 효과를 주는 기존의 방법을 응용하여 적용하였으며, B 프레임의 경우에는 참조 프레임에 대한 움직임 벡터를 기초로 구해진 움직임 보상 근사값을 이용하여 DCT 영역에서 장면 전환 효과를 주는 방법을 새로이 제안하였다. 이런 압축 영역에서의 장면 변화 적용 방법을 사용함으로써 압축을 해제하여 효과를 주는 것 보다 전환되는 프레임들의 화질을 유지하면서 3-4배 정도 빠르게 편집할 수 있다. 이러한 방법을 MPEG 비디오 편집기 시스템에 적용하게 된다면, 소프트웨어만을 이용한 저가의 데스크 탑 환경에서도 효율적인 비디오 편집 작업이 가능하게 될 것이다.

• 한국멀티미디어학회논문지
• /
• 제19권4호
• /
• pp.706-716
• /
• 2016

Each year, significant amounts of Stereoscopic 3D(S3D) contents have been introduced. However, viewers who enjoy the contents readily experience a sense of fatigue on account of various factors. Consequently, many improvement studies have been conducted with the domain of disparity by, for example, simply controlling the disparity or optimizing the reaction speed of viewers' eyes to vergence. However, such studies are limited to the disparity domain and therefore are restricted to a limited number of applications. In this study, we attempted to transcend this limitation and analyzed how a reconstruction in color and brightness, as well as disparity and other important features, affects eyes in terms of vergence adaptation. As a result, we found that, the higher the color similarity is, the better it positively affects vergence adaptation during viewing. Based on this analysis, we propose in this paper a similar color extraction method between takes that are applicable to real-life situations. In an evaluation, the algorithm was applied to publicly available S3D contents and produced a converted color optimized image. The vergence adaptation time of this applied contents was significantly decreased. Also it was minimized through color reconstruction, thereby, being resulted in enhancing viewer concentration.

• 대한전자공학회:학술대회논문집
• /
• 대한전자공학회 2002년도 하계종합학술대회 논문집(4)
• /
• pp.77-80
• /
• 2002

In this paper, We have studied for guaranteeing the clear display of MPEG-2 video sequence when conduct splicing of MPEG-2 system streams. we focus on the PES domain splicing considering video sequence. And we wish to make a base on the TS or PS domain splicing considering video sequence. For that, first, we compared and analyzed problems that is raised when different two PES streams are spliced and effects that affect the video sequence. And based on this analysis, we have searched for methods that resolve the cause of problems that can be happened in the display of video sequence directly in PES domain.

• Experimental and Molecular Medicine
• /
• 제50권12호
• /
• pp.1.1-1.14
• /
• 2018

DNA methylation is a regulatory mechanism in epigenetics that is frequently altered during human carcinogenesis. To detect critical methylation events associated with gastric cancer (GC), we compared three DNA methylomes from gastric mucosa (GM), intestinal metaplasia (IM), and gastric tumor (GT) cells that were microscopically dissected from an intestinal-type early gastric cancer (EGC) using methylated DNA binding domain sequencing (MBD-seq) and reduced representation bisulfite sequencing (RRBS) analysis. In this study, we focused on differentially methylated promoters (DMPs) that could be directly associated with gene expression. We detected 2,761 and 677 DMPs between the GT and GM by MBD-seq and RRBS, respectively, and for a total of 3,035 DMPs. Then, 514 (17%) of all DMPs were detected in the IM genome, which is a precancer of GC, supporting that some DMPs might represent an early event in gastric carcinogenesis. A pathway analysis of all DMPs demonstrated that 59 G protein-coupled receptor (GPCR) genes linked to the hypermethylated DMPs were significantly enriched in a neuroactive ligand-receptor interaction pathway. Furthermore, among the 59 GPCRs, six GI hormone receptor genes (NPY1R, PPYR1, PTGDR, PTGER2, PTGER3, and SSTR2) that play an inhibitory role in the secretion of gastrin or gastric acid were selected and validated as potential biomarkers for the diagnosis or prognosis of GC patients in two cohorts. These data suggest that the loss of function of gastrointestinal (GI) hormone receptors by promoter methylation may lead to gastric carcinogenesis because gastrin and gastric acid have been known to play a role in cell differentiation and carcinogenesis in the GI tract.

• 방송공학회논문지
• /
• 제5권2호
• /
• pp.188-198
• /
• 2000

편집 과정에서의 언더플로우의 문제는 이미 이전에 페이드-인 시퀀스를 삽입하여 해결하는 방법이 연구된 것이 있으나, 이는 원하지 않는 영상신호를 임의로 삽입한다는 점이 문제였다. 본 연구에서는 압축영역에서 수행할 수 있는. 시퀀스의 압축율에 따른 적절한 재양자화를 통한 기법을 적용하여 이러한 페이드-인 시퀀스 삽입 방법에서와 같은 문제점 없이 언더플로우를 해결하였다. 제안된 방법은 본래의 움직임 예측 영상에 재양자화에 의한 에러를 보상하여 재양자화로 인하여 발생하는 GOP 전체로의 영상에러의 흐름에 의한 화질 열화를 방지하여 PSNR에서 약 0.5~1dB 화질이 향상되었음이 실험 결과 확인되었다.

• 제어로봇시스템학회:학술대회논문집
• /
• 제어로봇시스템학회 1991년도 한국자동제어학술회의논문집(국내학술편); KOEX, Seoul; 22-24 Oct. 1991
• /
• pp.720-725
• /
• 1991

This paper is concerned with the program of the automatic mesh generation for 2-dimensional domain which contains the curved boundaries and holes. This program treats a new vertical - line drawing method. This method starts with 4-subdivisions of problem domain and the classification of the cross points of grid lines and boundaries. And the new node is generated by the vertical line to the line connecting the two intersections of a boundary and two grid lines in general. The boundaries are piecewise-curves composed of lines, circles, arcs, and free curves. The free curves are generated by B-Spline form. Although there were some bad elements for the complex boundary, it was possible to obtain the acceptable elements for the given boundaries. The results of automatic mesh generation can be verified directly by drawing on the computer monitor in executing the program. And it is possible to add the processes - that is, editing, hard copying, etc - using the script file in Auto-CAD.