• 한국식품과학회지
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• 제44권3호
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• pp.362-366
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• 2012

수산물 추출물로부터 간세포내 지질축적억제효과 추출물의 탐색을 통해 지방간 형성 억제 활성물질을 밝힐 목적으로, 각 48종의 수산물 추출물의 사람 간암세포유래인 HepG2 세포주에서의 중성지질축적억제 효과를 조사하였다. 그 결과, 18개의 추출물에서 유의적인 지질축적억제효과를 보였고, 그 중 Pagrus major, Octopus minor, Enteroctopus dofleini, Undaria pinnatifida Sporophyll, Styela clava, Enteromorpha intenstinalis 등 6개의 추출물이 유효한 효과가 있음을 관찰하였다. 이상의 결과를 바탕으로 지질대사에 관여하는 단백질들의 발현 정도를 조사하고, 활성물질의 분리 및 정제 등에 관한 연구와 지방간 형성 억제 효과에 대한 기전 연구가 진행되어야 할 것으로 사료된다.

• Fisheries and Aquatic Sciences
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• 제24권12호
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• pp.428-436
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• 2021

The aim of this study was to isolate and identify secondary metabolites from Sonchus brachyotus and evaluate their antioxidant and anti-inflammatory activities. In this study, we isolated three flavonoids from a 70% EtOH extract by Medium Pressure Liquid Chromatography (MPLC) and prep-High-Performance Liquid Chromatography (HPLC). To evaluate the biological activities (antioxidant and anti-inflammatory) of these flavonoids, their in vitro inhibitory activities against lipopolysaccharide (LPS)-induced reactive oxygen species (ROS) generation, nitric oxide (NO) production, and prostaglandin E₂ (PGE₂) secretion were determined. We successfully identified three flavonoids, namely luteolin (1), luteolin-7-O-β-D-glucoside (2), and luteolin-7-O-β-D-glucuronide (3) by spectral analyses. Luteolin (1) at 20 ㎍/mL inhibited ROS generation, NO production, and PGE₂ secretion by 48.6%, 61.28% and 12.10%, respectively, and luteolin-7-O-β-D-glucoside (2) inhibited NO and PGE₂ generation by 67.03% and 20.82%, respectively. Luteolin (1) and luteolin-7-O-β-D-glucoside (2) showed similar anti-inflammatory activities; however, luteolin (1) was observed to be a stronger antioxidant. Besides antioxidant and anti-inflammatory activities, S. brachyotus extract containing luteolin (1) and luteolin-7-O-β-D-glucoside (2) is considered to possess diverse biological activities. The results indicate that S. brachyotus is an edible medicinal plant, which is believed to be significant resource of functional foods.

• 수산해양교육연구
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• 제26권3호
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• pp.587-596
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• 2014

The study aims at suggesting the direction of industrial utilization for harmful jellyfish which appears in large quantities in the Korean coastal areas. If the technology of industrial utilization for the jellyfish is developed, it will be possible to grow the industry to the export industry due to the mass appearance of jellyfish in Korean coastal areas. The industrial utilization of jellyfish should follow the direction of minimizing the damage by the jellyfish blooms and maximizing the resource recycling of the jellyfish. Therefore, Korea needs construction of infrastructure for the industrialization and promotion of R&D in order to activate the industrial utilization. Finally, the study suggested issues and tasks for promoting the industrial utilization of jellyfish as follows. First, Korea should stabilize the high quality raw material supply system. Second, mass processing technology should be developed for the industrial utilization of the jellyfish. Third, research and manual development should follow for the preparation of turning the jellyfish to the food. Fourth, extraction technology of useful non-food substance such as extracting collagen from jellyfish should be developed and accelerate the industrial utilization of non-food area.

• 한국자원식물학회지
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• 제24권3호
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• pp.298-303
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• 2011

A large number of edible seaweeds are consumed by the coastal peoples of Asia. Some of them are used in traditional remedies in many parts of the world. In this study we investigated effects of supplementation with ethyl acetate extracts of the brown alga Eisenia bicyclis (EBE) on rat macrophage to evaluate the possibilities as immune-modulators. Twelve male SD rats were divided into two groups and the treatments were as follows: A, no Eisenia bicyclis extract (EBE) intake and distilled water ; B, oral supplemented with EBE 200 mg/kg. After 5 weeks of supplementation, rats were sacrificed to assess the effect on peritoneal macrophage functions. We showed no increasing effects on tumoricidal activity, phagocytic activity and NO production in macrophages in EBE supplementation group. However, EBE supplementation suppressed NO-iNOS production and p65 translocation into the nucleus in LPS-stimulated macrophages. Overall, these results suggest that the supplementation of EBE might have an anti-inflammatory effects on NO-iNOS production in macrophages throughout the inhibition of NF-${\kappa}B$ activation.

• 한국수산과학회지
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• 제10권3호
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• pp.151-162
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• 1977

분포량이 많고 식용되고 있는 중요 해조의 효율적인 이용과 영양학적인 의의를 구명하기 위한 기초자료를 얻기 위하여 동근돌김(Porphyra suborciculata). 잎파래(Enteromorpha linza). 톳(Hizikia jusiforme). 모자반(Sargassum fulvllum), 미역(Undaria pinnatifida), 청각(Codim fragile), 셀만모자반(Sargassum kjellmanianum), 구멍갈파래(Ulva pertusa)등 8종을 선정하여, 이들 해조의 불용성 단백질 추출에 영향을 주는 제요소 즉 조체처리별, 추출온도와 시간별, 시료일추출용매화, pH의 영향등을 검토하고 또한 추출액중의 단백질 심전조건에 대하여 검토하였다. 1. 식용 해조의 조단백질 함량은 둥근돌김(Porphyra suborbiculat) $24.14\%$, 미역(Undaria pinnatifida) $15.7\%$, 톳(Hizikia jusiforme) $7.66\%$, 모자반(Sargasum fulvellum) $12.61\%$, 셀만모자반(Sargassum kjellmanianum) $14.08\%$, 잎파래(Enteromorpha linza) $10.86\%$, 구멍갈파래(Ulva Pertusa) $16.60\%$, 청각(Codium fragile)$9.89\%$이었다. 2. 시료의 처리조건에 따른 추출효과는 dry icemethanol 한제 중에서 동결시켜, 해사를 첨가하여 마쇄한 시료가 수용성 단백질 추출성적이 월등하게 좋아, 막지사발에서 마쇄한 $1.5\~2.5$배의 추출성적을 보였다. 3. 시료일추출용매비는 점질물이 적은 구멍갈파래, 잎파래는 1:20(w/v)에서, 점질물량이 많은 청각,미역, 톳, 모자반, 둥근돌김 등은 1:30(w/v)에서, 셀만모자반은 1:40(w/v)일 때 추출성적이 좋았다. 4. 추출시간은 구멍갈파래, 잎파래는 1시간, 둥근돌김, 톳, 미역, 모자반, 셀만모자반은 2시간, 청각은 3시간 정도에서 가장 효과적이었다. 5. 추출온도는 톳이 $40^{\circ}C, 둥근돌김, 청각, 잎파래 미역, 모자반, 셀만모자반 $50\%$에서, 구멍갈파래는 $60^{\circ}C$에서 최고의 추출성적을 보였다. 6. pH의 영향은 각 시료마다 대동소이하여 $pH9\~12$에서 가장 좋은 결과를 얻었다. 7. 추출 최적조건에서의 수용성 단백질 추출율은 1차 추출에서 전조단백질에 대하여 평균 $63\%$, 2차 유출에서 평균 $8.6\%$ 합계 $74\%$ 정도의 수용성 단백질 추출이 가능했으며, 1차에 대한 2차의 추출비율이 7.5:1이었다. 8. 단백질 침전분리 조작으로는 Barnstein method, methanol 처리법, TCA 처리법, 가열처리법 순으로 침전효과가 있었다.