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Antioxidant Activities of Extracts from Fermented Mulberry (Cudrania tricuspidata) Fruit. and Inhibitory Actions on Elastase and Tyrosinase (꾸지뽕열매 발효 추출물의 항산화 특성과 Elastase 및 Tyrosinase 저해활성)

  • Kang, Dae-Hun;Kim, Jae-Won;Youn, Kwang-Sup
    • Food Science and Preservation
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    • v.18 no.2
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    • pp.236-243
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    • 2011
  • We evaluated the nutritional value of 70% ethanol extracts (EE) from fermented cudrania tricuspidata fruit (ECT: EE of C. tricuspidata, EFCT: EE of fermented C. tricuspidata, EFCTL: EE of fermented C. tricuspidata by B. licheniformis, EFCTS: EE of fermented C. tricuspidata by B. subtilis) by determined the total polyphenol and flavonoid contents, antioxidant activities, and inhibitory actions on elastase and tyrosinase. The yields of freeze-dried powder of ECT, EFCT, EFCTL, and EFCTS were 54.22%, 54.43%, 57.51%, and 57.23% (each w/w), respectively. The color of $L^*,\;a^*,\;b^*$ values decreased of fermented cudrania tricuspidata. The fermented EFCTL and EFCTS had relatively higher contents of total polyphenol and flavonoid than those of other extracts. ABTS radical scavenging ability were 94.61%, 95.85%, 94.36%, and 96.69%, respectively. SOD (superoxide dismutase)-like activities were in the order EFCT (32.60%) > EFCTS (27.10%) > EFCT (23.30%) > ECT (22.00%), and nitrite scavenging activity was in order of EFCTS (51.18%) > EFCTL (45.61%) > EFCT (41.93%) > ECT (27.76%), respectively. Ferrous ion chelating activity of EFCTL (67.34%) and EFCTS (60.36%) was significantly the highest, whereas ECT (52.34%) and EFCT (51.73%) had not different significantly. Xanthine oxidase, elastase and tyrosinase inhibitory activities at 0.5% (w/v) solutions of EFCTL and EFCTS were somewhat higher than those of non-fermented ECT. In conclusion, we provide experimental evidence that extracts of fermented cudrania tricuspidata of B. subtilis and B. licheniformis exhibited higher antioxidant activities and inhibitory actions on elastase and tyrosinase compared with non fermented cudrania tricuspidata.

Evaluation of Nondestructive Evaluation Size Measurement for Integrity Assessment of Axial Outside Diameter Stress Corrosion Cracking in Steam Generator Tubes (증기발생기 전열관 외면 축균열 건전성 평가를 위한 비파괴검사 크기 측정 평가)

  • Joo, Kyung-Mun;Hong, Jun-Hee
    • Journal of the Korean Society for Nondestructive Testing
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    • v.35 no.1
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    • pp.61-67
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    • 2015
  • Recently, the initiation of outside diameter stress corrosion cracking (ODSCC) at the tube support plate region of domestic steam generators (SG) with Alloy600 HTMA tubes has been increasing. As a result, SGs with Alloy600 HTMA tubes must be replaced early or are scheduled to be replaced prior to their designed lifetime. ODSCC is one of the biggest threats to the integrity of SG tubes. Therefore, the accurate evaluation of tube integrity to determine ODSCC is needed. Eddy current testing (ECT) is conducted periodically, and its results could be input as parameters for evaluating the integrity of SG tubes. The reliability of an ECT inspection system depends on the performance of the inspection technique and abilty of the analyst. The detection probability and ECT sizing error of degradation are considered to be the performance indices of a nondestructive evaluation (NDE) system. This paper introduces an optimized evaluation method for ECT, as well as the sizing error, including the analyst performance. This study was based on the results of a round robin program in which 10 inspection analysts from 5 different companies participated. The analysis of ECT sizing results was performed using a linear regression model relating the true defect size data to the measured ECT size data.

The Prediction of Blending Ratio of Cut Tobacco, Expanded Stem, and Expanded Cut Tobacco in Cigarettes using Near Infrared Spectroscopy (근적외분광법을 이용한 권련 중 일반각초, 팽화주맥 및 팽화각초 배합비 분석)

  • 김용옥;정한주;김기환
    • Journal of the Korean Society of Tobacco Science
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    • v.22 no.1
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    • pp.76-83
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    • 2000
  • This study was carried out to predict blending ratio of cut tobacco(CT), expanded stem(ES), and expanded cut tobacco(ECT) in cigarettes. CT, ES, and ECT samples from A brand were, ground and blended with reference to A blending ratio, and scanned by near infrared spectroscopy(NIRSystem Co., Model 6500). Calibration equations were developed and then determined blending ratio by NIRS. The standard error of calibration(SEC) and performance(SEP) of C factory samples between NIRS and known blending ratio were 0.97%, 1.93% for CT, 0.50%, 1.12 % for ES and 0.68%, 1.10% for ECT, respectively. The SEP of CT, ES and ECT of Band D factory samples determined by C factory calibration equation were more inaccurate than those of C factory samples determined by C factory calibration equations. These results were caused by the difference of CT, ES and ECT spectra followed by each factory. The SEP of CT, ES and ECT of Band D factories determined by calibration equations derived from each factory samples were more accurate than those of determined by calibration equation derived from C factory samples. Each factory SEP of CT, ES and ECT determined by calibration equation derived from all calibration samples(B+C+D factory) was similar to that determined by calibration equation derived from each factory samples. To improve the analytical inaccuracy caused by spectra difference, we need to apply a specific calibration equation for each factory sample. Data in development of specific calibrations between sample and NIRS spectra might supply a method for rapid determination of blending ratio of CT, ES, and ECT.

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Effect of two types of muscle contraction training on muscle thickness, strength, and delayed onset of muscle soreness in persons with chronic stroke

  • Lim, Seung-yeop;Lee, Wan-hee
    • Physical Therapy Rehabilitation Science
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    • v.7 no.4
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    • pp.154-163
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    • 2018
  • Objective: The purpose of this study was to investigate the effects of eccentric contraction training (ECT) and concentric contraction training (CCT) on the muscle thickness (MT), muscle strength (MS) and delayed onset of muscle soreness (DOMS) of the lower extremities in persons with chronic stroke. Design: Randomized controlled trial. Methods: Thirty persons with chronic stroke were randomly assigned to the ECT or the CCT group. The ECT was performed in a specially designed system of eccentric contraction of both legs and, the CCT was performed using a traditional stepper system for concentric contraction of both legs. The training was performed for 30 min/times, 3 times/wk for 6 weeks. Rehabilitation ultrasound imaging was used to measure MT of the vastus medialis/lateralis (VL), and soleus (SOL), a digital muscle tester was used to measure MS, and a visual analog scale was used to assess DOMS. Results: In the ECT group, MT was significantly improved except for SOL resting (p<0.01). In the CCT group, the MT was significantly improved except for VL contraction (p<0.05). The MS was significantly improved in both groups, especially in the ECT group (p<0.01). In the ECT group, muscle soreness was highest in the first week after training but gradually decreased, and in the CCT group, it was highest in the second week of training but gradually decreased (p<0.01). Conclusions: ECT can improve lower limb MT, MS, and DOMS of chronic stroke survivors. Therefore, it is recommended that ECT be used in the rehabilitation of persons with chronic stroke.

The Electroconvulsive Therapy in the Prevention of Suicide Risks and Attempts (자살 위험성 및 자살 시도 방지에 대한 전기경련치료의 역할)

  • Kim, Hee Cheol;Jeong, Seong Hoon;Ahn, Yong Min;Park, Seung Hyun;Kim, Yong Sik;Chung, In Won
    • Korean Journal of Biological Psychiatry
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    • v.27 no.2
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    • pp.64-73
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    • 2020
  • Suicidality is the most serious complication of mood disorders and psychosis; effective treatment should reduce suicide rates. The Organization for Economic Cooperation and Development age-standardized suicide rate in Korea was 22.6 in 2018, much higher compared to other countries worldwide. As mental and psychiatric problems are the main reasons for suicide attempts, accounting for 31.6% in 2018, targeting such problems should be the focus of efforts to reduce suicide rates. However, the ability of current pharmacotherapeutic and psychotherapeutic interventions to reduce suicide rates is limited due to their delayed effects. Therefore, electroconvulsive therapy (ECT) has been proposed as an alternative treatment. This approach is effective for treating most mental disorders associated with high suicide rates, including severe depression, bipolar disorder, and intractable psychotic disorders; ECT is also effective for Parkinson's disease, which has the highest suicide rate among all disorders in Korea. The acute, long-term, and prophylactic effects of ECT on suicidality have been reported in the literature, and treatment guidelines outside of Korea recommend that ECT be used at an early stage for rapid reduction of suicide rates, as opposed to being applied as a treatment of last resort. However, only ~0.092 of every 10000 members of the Korean general population received ECT in 2018; this is much lower than the average rate worldwide, of 2.2 per 10000. Elimination of obstacles to the use of ECT, early crisis intervention involving administration of ECT for rapid stabilization, and maintenance ECT to prevent recurrence should reduce suicide rates.

Eddy Current Signal Analysis for Transmit-Receive Pancake Coil on ECT Array Probe

  • Lee, Hyang-Beom
    • Journal of the Korean Society for Nondestructive Testing
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    • v.26 no.1
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    • pp.25-29
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    • 2006
  • In this paper, the eddy current signals come from a pair oi transmit-receive (T/R) pancake coil on ECT array Probe are analyzed with the variations of the lift-of and of the distance between transmit and receive coils. To obtain the electromagnetic characteristics of the probes, the governing equation describing the eddy current problems is derived from Maxwell's equation and is solved using three-dimensional finite element method. Eddy current signals from T/R coils on ECT array probe have quite different characteristics compared with ones from impedance coil on rotating pancake coil probe. The results in this paper ran be helpful when the field eddy current signals from ECT array probe are evaluated.

Defect Signal Analysis of Steam Generator Tube in NPP Using ECT Array Probe (ECT Array Probe를 이용한 원전 SG세관의 결함 신호해석)

  • Lim, Geon-Gyu;Kim, Ji-Ho;Lee, Hyang-Beom
    • Proceedings of the KIEE Conference
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    • 2008.07a
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    • pp.772-773
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    • 2008
  • 본 논문에서는 ECT Array Probe를 이용한 원자력 발전소의 SG세관의 결함 신호를 해석하였다. 프로브의 전자기적 특성을 해석하기 위하여 맥스웰 방정식을 이용하여 지배방정식을 유도하였고, 3차원 유한요소법을 이용하여 전자기 수치 해석을 수행하였다. 신호해석을 위해 사용된 결함의 종류는 FBH결함이며, 결함의 깊이는 세관 두께의 40[%] 및 100[%]로 하였다. 시험주파수는 300[kHz], 400[kHz]를 사용하였으며, 각각의 시험주파수에 대한 결과를 비교 분석하였다. 해석결과 결함부위에서 신호의 증가를 확인할 수 있었으며, 주파수 시험변화시 300[kHz]보다 400[kHz]일때 결함 신호가 증가하는 것을 확인할 수 있었다. 또한 획득한 신호를 ASME 표준 시험편을 이용한 ECT Array Probe의 와전류탐상 실험신호와 비교하였다. 본 논문의 결과는 ECT Array Probe를 이용하여 원전 SG세관 검사시 결함신호해석에 도움이 될 것으로 사료된다.

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Social Stigmata of Electroconvulsive Therapy and Their Reduction Strategies (전기경련치료에 대한 사회적 낙인과 해소 방안)

  • Lee, Junhee;Kim, Minah;Kim, Se Hyun;Lee, Yu Sang;Kwon, Jun Soo
    • Korean Journal of Biological Psychiatry
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    • v.27 no.2
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    • pp.37-41
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    • 2020
  • Modified electroconvulsive therapy (ECT) which started in 1950s is a safe and efficacious treatment for several mental disorders including mood disorders and psychotic disorders. However, its usage in present days is still limited by misconceptions and stigmata of ECT. This paper overviews the background from which the stigmata of ECT stemmed and the current status of stigmata surrounding ECT among the public and medical professionals. In addition, a few potential strategies for reducing stigmata of ECT are provided in this review.

Practical Considerations in Anesthesia for Electroconvulsive Therapy (전기경련치료의 마취를 위한 실제적 문제들)

  • Youn, Tak;Kim, Yong Sik;Lee, Nam Young;Kim, Se Hyun;Choi, Jun Gwon;Lee, Jeoung Hyuk;Chung, In Won
    • Korean Journal of Biological Psychiatry
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    • v.24 no.3
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    • pp.110-128
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    • 2017
  • Electroconvulsive therapy (ECT) has been recognized effective as primary or secondary treatments for major psychiatric disorders including depression and schizophrenia, as well as psychiatric emergency such as suicide, food refusal and catatonia, and so on. Medicines used in anesthetic induction for ECT, cause various reactions in autonomous, hemodynamic, and neuromuscular systems. The anesthetics also affect the duration, threshold, and intensity of seizures evoked with electric stimuli, and thus modify the seizure quality in ECT. Individual characteristics of age, sex, weight, comorbid physical disorders, and medications should also be considered for optimal clinical response after ECT. When preparing for anesthesia, adequate anesthetic agents and muscle relaxants, and rapid recovery should be carefully considered. We conducted a case-series study to address practical issues that are frequently encountered during ECT anesthesia with reviews of updated journals in order to provide practical helps to clinicians who are preparing ECT for their patients.

Esculetin Inhibits Adipogenesis and Increases Antioxidant Activity during Adipocyte Differentiation in 3T3-L1 Cells

  • Kim, Younghwa;Lee, Junsoo
    • Preventive Nutrition and Food Science
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    • v.22 no.2
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    • pp.118-123
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    • 2017
  • This study was conducted to investigate the anti-adipogenic activity of esculetin (ECT) which is reported to be attributable to the modulation of antioxidant enzymes during adipogenesis. After six days of ECT treatment of 3T3-L1 cells, lipid accumulation was determined by Oil red O staining. The levels of glutathione (GSH) and reactive oxygen species (ROS), and the activities of antioxidant enzymes including glutathione reductase, glutathione peroxidase (GPx), and catalase were examined. In addition, the protein expression of glutamate-cysteine ligase catalytic subunit (GCLC) and heme oxygenase-1 (HO-1) was measured by Western blot. ECT significantly inhibited lipid accumulation by approximately 80% and ROS production in a concentration-dependent manner. GSH level and GPx activity were increased by ECT by approximately 1.3-fold and 1.7-fold compared to the control group, respectively. GCLC and HO-1 expression were elevated by ECT. These results showed that ECT treatments strongly inhibit adipogenesis, increase GSH level, and upregulate the expression of GCLC and HO-1, possibly by decreasing ROS production in 3T3-L1 cells during adipogenesis.