• Title/Summary/Keyword: early-late phase

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Morphometrics of Scinaia latifrons (Nemaliales, Rhodophyta) in the Southwestern Gulf of California, Mexico

  • Leon-Cisneros, Karla;Riosmena-Rodriguez, Rafael
    • ALGAE
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    • v.20 no.1
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    • pp.31-36
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    • 2005
  • Scinaia latifrons Howe occurs in rhodolith beds between 9 to 27 m depths at San Lorenzo channel, Gulf of California, Mexico. As very little was known about the morphometrics of this normally temperate species in a subtropical area, we investigated the phenological changes from December 1998 to December 1999. The gametophytic phase of the species was present from late February to late May, which represented a shorter time period to other known species in the genus. Our results suggested that two gametophytic cohorts develop over winter and early spring based on the size class structure of the frond height. Thalli became reproductively mature at a small size (1-2 cm in height) and all the plants were monoecious. Scinaia latifrons at the study location underwent allometric growth, indicated by the lack of correlation between size, width of the plants, or branching patterns.

Cell Differentiation and Ultrastructure of the Seminiferous Epithelium in Myotis macrodactylus (큰발웃수염박쥐 (Myotis macrodactylus)의 정상피세포의 분화와 미세구조)

  • Lee, Jung-Hun
    • Applied Microscopy
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    • v.33 no.1
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    • pp.25-39
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    • 2003
  • Cell differentiation and ultrastructural characteristics in the seminiferous epithelium of Myotis macrodactylus was investigated with the light and electron microscopes. Spermatogenesis has begun at April and finished at September. The nuclei of A spermatogonia (dark and pale type of spermatogonia) were oval, applied to the basal lamina, and surrounded by Sertoli cells. By comparison with other types of spermatogonia, the cell and nucleus of B type of spermatogonium is globular and larger than A types of spermatogonia. The nucleolus appears as a coarse and touches the nuclear membrane. The cell and nucleus of spermatocytes was globular and larger, but primary spematocyte is larger than secondary spermatocyte. Spermiogenesis was divided according to the level of fine structural difference, into Golgi, cap, acrosomal, maturation and spermiation phases; Golgi, cap, acrosomal and spermiation phases were further subdivided into steps of early and late phase respectively, and maturation phase has only one step. Hence, the spermiogenesis has been divided into a total of nine phases. In the change of karyoplasm, the chromatin granules are condensed at late Golgi phase and completed at spermiation phase. The sperm tail began to develop in early Golgi phase and completed in spermiation phase. The process of degeneration of spermatogenic cells in the seminiferous tubules was continually observed from October, before the beginning of hibernation, to hibernation phase (November, December, January, February, March). Immatured spermatogenic cells in the seminiferous tubules have been engulfed by phagocytosis of Sertoli cells during period of degeneration. It is deduced that the adaptative strategy serves as the mechanism to regulate the effective use of energy to prepare for long hibernation and regulation of breeding cycle.

A Multi-Wavelength Study of Galaxy Transition in Different Environments (다파장 관측 자료를 이용한 다양한 환경에서의 은하 진화 연구)

  • Lee, Gwang-Ho
    • The Bulletin of The Korean Astronomical Society
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    • v.43 no.1
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    • pp.34.2-35
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    • 2018
  • Galaxy transition from star-forming to quiescent, accompanied with morphology transformation, is one of the key unresolved issues in extragalactic astronomy. Although several environmental mechanisms have been proposed, a deeper understanding of the impact of environment on galaxy transition still requires much exploration. My Ph.D. thesis focuses on which environmental mechanisms are primarily responsible for galaxy transition in different environments and looks at what happens during the transition phase using multi-wavelength photometric/spectroscopic data, from UV to mid-infrared (MIR), derived from several large surveys (GALEX, SDSS, and WISE) and our GMOS-North IFU observations. Our multi-wavelength approach provides new insights into the *late* stages of galaxy transition with a definition of the MIR green valley different from the optical green valley. I will present highlights from three areas in my thesis. First, through an in-depth study of environmental dependence of various properties of galaxies in a nearby supercluster A2199 (Lee et al. 2015), we found that the star formation of galaxies is quenched before the galaxies enter the MIR green valley, which is driven mainly by strangulation. Then, the morphological transformation from late- to early-type galaxies occurs in the MIR green valley. The main environmental mechanisms for the morphological transformation are galaxy-galaxy mergers and interactions that are likely to happen in high-density regions such as galaxy groups/clusters. After the transformation, early-type MIR green valley galaxies keep the memory of their last star formation for several Gyr until they move on to the next stage for completely quiescent galaxies. Second, compact groups (CGs) of galaxies are the most favorable environments for galaxy interactions. We studied MIR properties of galaxies in CGs and their environmental dependence (Lee et al. 2017), using a sample of 670 CGs identified using a friends-of-friends algorithms. We found that MIR [3.4]-[12] colors of CG galaxies are, on average, bluer than those of cluster galaxies. As CGs are located in denser regions, they tend to have larger early-type galaxy fractions and bluer MIR color galaxies. These trends can also be seen for neighboring galaxies around CGs. However, CG members always have larger early-type fractions and bluer MIR colors than their neighboring galaxies. These results suggest that galaxy evolution is faster in CGs than in other environments and that CGs are likely to be the best place for pre-processing. Third, post-starburst galaxies (PSBs) are an ideal laboratory to investigate the details of the transition phase. Their spectra reveal a phase of vigorous star formation activity, which is abruptly ended within the last 1 Gyr. Numerical simulations predict that the starburst, and thus the current A-type stellar population, should be localized within the galaxy's center (< kpc). Yet our GMOS IFU observations show otherwise; all five PSBs in our sample have Hdelta absorption line profiles that extend well beyond the central kpc. Most interestingly, we found a negative correlation between the Hdelta gradient slopes and the fractions of the stellar mass produced during the starburst, suggesting that stronger starbursts are more centrally-concentrated. I will discuss the results in relation with the origin of PSBs.

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BCL-2 and BAX Expression in Normal Human Endometrium (인간의 정상 자궁내막조직에서의 BCL-2와 BAX 단백질의 발현)

  • Hong, Soon-Oak;Lee, Byung-Seok;Yang, Woo-Ick;Lee, Jee-Sung;Cha, Dong-Hyon;Cho, Yong-Seon;Kim, Jeong-Yeon;Park, Ki-Hyun;Cho, Dong-Jae;Song, Chan-Ho
    • Clinical and Experimental Reproductive Medicine
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    • v.27 no.3
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    • pp.245-251
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    • 2000
  • Objective: To investigate the distribution of BCL-2, BAX proteins and DNA fragmented cells in the normal human endometrium during at each menstrual cycle in order to find out whether apoptosis regulates cyclic endometrial change. Methods: Normal endometrial tissues were obtained from 40 patients, $32{\sim}45$ year of age, all with regular menstrual cycle, who were undergoing abdominal hysterectomy for myoma of uterus or cervical intraepithelial neoplasia for the period from 1992 through 1997. Immunohistochemical staining was used to determine the expression of BCL-2 and BAX protein with paraffin-embedded tissues. Results: BCL-2 was expressed on the glandular epithelial cells and stromal cells during the proliferative phase. The intensity of BCL-2 was increased predominantly on the basal layer than the functional layer in late proliferative phase. However, BCL-2 immunoreactivity was decreased in the secretory phase. BAX was expressed predominantly during the secretory phase. The intesity was increased in late secretory phase rather than early secretory phase. DNA fragmented cells were detected in a few cells at each phase. However, it was increased during the late secretory phase. Conclusion: Apoptosis-related genes, BCL-2 and BAX, may play a role in the regulation of cyclic endometrial change.

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Attribution of Cortical Granules to Formation of Fertilization Envelopes and Polyspermy Block in Urechis unicinctus

  • Shin, Kil-Sang;Kwon, Hyuk-Jae;Kim, Wan-Jong
    • Animal cells and systems
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    • v.9 no.2
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    • pp.57-64
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    • 2005
  • Cortical reaction and polyspermy block are well defined in most marine invertebrates. In Urechis species, the function of cortical granules (CGs) is not yet known, and there is controversy on whether the cortical reaction occurs, or the fertilization envelope (FE) is attributed to CG releases or functions to prevent polyspermy. This study was carried out to determine the cortical reactions and functions of the FE in Urechis unicinctus. Artificial insemination of the eggs revealed that CG release occurred to give rise to perivitelline space (PS) into the final FE. Both PS and final FE effectively blocked polyspermy. The final FE was accomplished within 10 min after sperm-egg initial binding. No massive release of CGs occurred within the early phase of 5 min after the initial binding, initially and the PS seemed to playa role to prevent polyspermy. The CG massively released its content into the PS in late phase of FE formation, and differentiated PS into five intermediate layers. The layers opened into each other by anastomosis, so that the final FE consisted of two layers, the inner layer ($15{\mu}m$ in thickness) and the outer layer ($1{\mu}m$ in thickness). The outer layer derived from vitelline layer and the inner layer consisted of PS and CG secretions. Immunofluorescence and confocal laser microscopy revealed that the spermatozoon took up residence in the egg cortex during FE formation and successive meioses of the fertilized egg. These results suggest that both PS and final FE of U. unicinctus were equivalent to the early and late block, respectively, of other marine animals.

A study of analgesic effect of Zanthoxylum bungeanum Maxim pharmacopuncture

  • Lee, Jung Hee;Lee, Yun Kyu;Lee, Hyun Jong;Lee, Bong Hyo;Kim, Jae Soo
    • Journal of Acupuncture Research
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    • v.34 no.2
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    • pp.61-74
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    • 2017
  • Objectives : This study was carried out to evaluate analgesic effects of Zanthoxylum bungeanum Maxim (ZM) pharmacopuncture on formalin-induced pains in Sprague-Dawley (SD) rats and ICR-mice. Methods : The subjects were divided 8 weeks aged rats with constant pain sensitivity into five groups; normal (treated with normal saline at Taegye (KI3) and before injected with normal saline at hindpaw), Con-1 (treated with normal saline at KI3 before injected with formalin at hindpaw), Lido-1 (treated with lidocaine at KI3), ZMWG-1 (treated with Hot water extraction pharmacopuncture of Zanthoxylum bungeanum Maxim at KI3), ZMEG-1 (treated with ethanol extraction pharmacopuncture of Zanthoxylum bungeanum Maxim at KI3). After 35 minutes, we measured ultrasonic vocalization (USV) and enzyme activities of both Aspartate aminotransferase (AST), Alanine aminotransferase (ALT) in rat serum. In addition, Tail flick test is performed by injecting ICR mice at 5 weeks of age. And it classified into 4 groups (Con-2, Lido-2, ZMWG-2, ZMEG-2) according to the kind of drug (normal saline, lidocaine, ZMW, ZME). After each drug injection, we examined the reaction by placing the tail in water at $50^{\circ}C$. Results : ZME had analgesic effects in the early and late phase of USV during the formalin test. There were no significant differences between ZMEG-1 and Lido-1 in early and late phase of USV. Also, No significant differences observed in serum AST and ALT activity in ZMWG-1 and ZMEG-1 compared with Con-1. For tail-flick test, analgesic effect on warmth significantly increased in Lido-2 and ZMEG-2 compare to that of Con-2. Conclusion : ZME pharmacopuncture had analgesic effects on formalin-induced pain without liver toxicity. Also, tail-flick test suggest that ZME pharmacopuncture could be useful technique on analgesic effect on warmth and treatment of pains.

Conceptus-derived cytokines interleukin-1β and interferon-γ induce the expression of acute phase protein serum amyloid A3 in endometrial epithelia at the time of conceptus implantation in pigs

  • Soohyung Lee;Inkyun Yoo;Yugyeong Cheon;Hakhyun Ka
    • Animal Bioscience
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    • v.36 no.3
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    • pp.441-450
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    • 2023
  • Objective: Serum amyloid A3 (SAA3), an acute phase response protein, plays important roles in opsonization, antimicrobial activity, chemotactic activity, and immunomodulation, but its expression, regulation, and function at the maternal-conceptus interface in pigs are not fully understood. Therefore, we determined the expression of SAA3 in the endometrium throughout the estrous cycle and at the maternal-conceptus interface during pregnancy. Methods: Endometrial tissues from pigs at various stages of the estrous cycle and pregnancy and with conceptuses derived from somatic cell nuclear transfer (SCNT), conceptus tissues during early pregnancy, and chorioallantoic tissues during mid- to late pregnancy were obtained and the expression of SAA3 was analyzed. The effects of the steroid hormones, interleukin-1β (IL1B), and interferon-γ (IFNG) on the expression of SAA3 were determined in endometrial explant cultures. Results: SAA3 was expressed in the endometrium during the estrous cycle and pregnancy, with the highest level on day 12 of pregnancy. The expression of SAA3 in the endometrium was significantly higher on day 12 of pregnancy than during the estrous cycle. Early-stage conceptuses and chorioallantoic tissues during mid to late pregnancy also expressed SAA3. The expression of SAA3 was primarily localized to luminal epithelial cells in the endometrium. In endometrial explant cultures, the expression of SAA3 was induced by increasing doses of IL1B and IFNG. Furthermore, the expression of SAA3 decreased significantly in the endometria of pigs carrying conceptuses derived from SCNT on day 12 of pregnancy. Conclusion: These results suggest that the expression of SAA3 in the endometrium during the implantation period increases in response to conceptus-derived IL1B and IFNG. The failure of those appropriate interactions between the implanting conceptus and the endometrium leads to dysregulation of endometrial SAA3 expression, which could result in pregnancy failure. In addition, SAA3 could be a specific endometrial epithelial marker for conceptus implantation in pigs.

The Hormonal Levels of the Short Luteal Phase in Korean Women(II);Change of Serum Prolactin (황체기단축현상을 가진 한국여성의 호르몬양(II);혈청 Prolactin의 변동)

  • Yoon, Yong-Dal;Lee, Joon-Yeong
    • Clinical and Experimental Reproductive Medicine
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    • v.11 no.2
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    • pp.5-15
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    • 1984
  • 본 연구는 황체기 단축현상(Short luteal phase, SLP) 을 가진 한국여성의 월경주기내 Prolactin 호르몬의 농도변화를 정상월경주기 (Normal luteal phase, NLP)와 비교하고져 하였다. 한편, Circadian rhythm 내 PRL 농도의 변화를 비교하고 임신기간중 PRL의 농도변화를 방사면역측정법(RIA) 으로 조사하였다. SLP여성의 PRL 농도는 후기의 여포성숙기(Late follicular phase) 및 초기황체형성기(Early IuteaI phase) 정상에 비하여 현저히 낮았다. 정상 월경주기에서는 PRL/Progesterone(mU/1/mg/ml)의 비율이 후기 여포성숙기까지 증가한 후 LH Peak day 이후 감소하는데 반하여 SLP여성의 비율은 조기 여포성숙기부터 계속적인 감소현상을 보였다. 또한 취침전 (04:00시) PRL의 농도는 SLP여성에게는 현저히 낮았다. 임신주기중 PRL의 농도는 4개월째 1,077mU/l 에서부터 9개월째 4,462mU/l까지 점진적인 증가를 하였다. 위의 결과로 보아 황체기단축현상은 PRL의 분비이상이 하나의 주요요인이 될 수 있으며, 특히 PRL/Progesterone의 농도비율이 SLP의 판정에 한 지표가 될 수 있을 것으로 사료된다.

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Launch and Early Orbit Phase Simulations by using the KOMPSAT Simulator

  • Lee, Sanguk;Park, Wan-Sik;Lee, Byoung-sun;Lee, Ho-Jin;Park, Hanjun
    • 제어로봇시스템학회:학술대회논문집
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    • 1999.10a
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    • pp.33-36
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    • 1999
  • The KOMPSAT, which is scheduled to be launched by Taurus launch vehicle in late November of 1999, will be in a sun-synchronous orbit with an altitude of 685km, eccentricity of 0.001, inclination of 98deg and local time of ascending node of 10:50 a.m. Electronics and Telecommunications Research Institute and Daewoo Heavy Industry had jointly developed a KOMPSAT Simulator as a component of the KOMPSAT Mission Control Element. The MCE had been delivered to Korea Aerospace Research Institute for the KOMPSAT ground operation. It is being used for training of KOMPSAT ground station personnel. Each of satellite subsystems and space environment were mathematically modeled in the simulator. To verify the overall function of KOMPSAT simulator, a Launch and Early Orbit Phase(LEOP) operation simulations have been performed. The simulator had been verified through various tests such as functional level test, subsystem test, interface test, system test, and acceptance test. In this paper, simulation results for LEOP operations to verify flight software adapted into simulator, satellite subsystem models and environment models are presented.

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Motion Analysis of Tresidmill Walking with Various Slopes at a Normal Speed (Treadmill에서의 경사로 정상보행에 관한 동작분석)

  • Kim, Youngho;Yang, Giltae;Mun, Museong
    • Journal of Biomedical Engineering Research
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    • v.18 no.1
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    • pp.71-78
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    • 1997
  • Kinematic and kinetic studies were performed to investigate the walking characteristics on a treadmill with various slopes at the same speed of 1.25m/sec. Six different slopes of the treadmill were selected . -4%(-$2.3^{\circ}$), 0%($0^{\circ}$), 5%($2.9^{\circ}$), 10%($5.7^{\circ}$), 15%($8.6^{\circ}$), and 20%($11.3^{\circ}$). With increased slopes of the treadmill, both hip and knee flexion angles significantly increased at initial contact, and the maximum hip flexion during swing phase and the maximum knee flexion during stance phase also significantly increased Ankle dorsiflexion angle at initial contact and the maximum dorsiflexion increased with increased slopes. However, the maximum plantarflexion in early swing was slightly reduced with increased slopes. Hip extension in late stance and the maximum knee flexion in early swing was not changed sigilificantly with increased slopes. As for the vertical ground reaction force, compared to the yond level walking, both the first and the second peak forces increased, but the mid-support force decreased.

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