• Title/Summary/Keyword: early-late phase

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Spermiogenesis in the Korean Daubenton's Bat(Myotis daubentonii ussuriensis) (한국산 물윗수염박쥐(Myotis daubentonii ussuriensis)의 정자변태)

  • 손성원
    • Development and Reproduction
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    • v.1 no.1
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    • pp.9-24
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    • 1997
  • To investigate the process of spermiogenesis of the Korean eastern Daubenton's bat, Myotis daubentonii ussuriensis, the testis obtained from mature male bats was studied by transmission electron microscope and were based on the variety and diagnostic characters of cell organells. The results obtained from the present study are as follows. According to the differentiation of the cell organells, the spermiogenesis of the Korean eastern Daubenton's bat, M. d. ussuriensis, was divided into Golg, cap, acrosome, maturation and spermiation phases. Besides, these Golgi, cap, acrosome, and maturation phase were subdivided into the steps of early and late phases repectively and matruation phase was subdivided into step of early, mid and late phases. Therfore, the spermiogenesisof M. d. ussuriensis has been divided into a total of 11 phases. The chromatin granules began to condense at the early cap phase, regularized at the acrosome phase, and a perfect nucleus of sperm was formed at the maturation phase. The chromatoid body was occurred in the upper cytoplasm of nucleus at the early Golgi phase, and it was accurred the posterior cytoplasm of the nucleus at the early maturatio phase. The formation of sperm tail began to be develop in the early golgi phase, and completed at the spermiation phase. The fiber structure of middle piece was consisted of nine outer doublets and two central singlet microtubules and Nos. 1, 5, 6 and 9 in the outer dense were larger than the others(2, 3, 4, 7, 8).

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Effect of IPTG Induction on Production of ${\beta}$-Galactosidase-PreS2 Fusion Protein in Recombinant Escherichia coli

  • Nam, Soo-Man;Park, Young-Hoon
    • Journal of Microbiology and Biotechnology
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    • v.1 no.4
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    • pp.274-280
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    • 1991
  • Effects of IPTG induction on cell growth and production of ${\beta}$-galactosidase-preS2 fusion protein (${\beta}$gal-preS2) were studied in a defined medium using a recombinant Escherichia coli JM109/pCMHB30. IPTG was added (0.2 mM) to induce the cloned-gene expression in the early-, mid-, and late-log growth phases. The most serious decreases in growth rate and plasmid stability were observed for the induction in the early-log growth phase. The expression level of ${\beta}$gal-preS2 attained by the induction in the mid-log phase was about 0.51 mg fusion protein/mg total cellular protein, which was 2- and 5-fold improvement over the levels obtained with the inductions in the early- and late-log phases. Formation of acidic byproducts including acetate and pyruvate showed different profiles during the fermentation period for each cases of induction; pyruvate was the major byproduct for the induction in the early-log phase while acetate production became more significant for the cases of inductions in the mid- and late-log phases.

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STUDIES ON RESPONSES OF THE RICE PLANT TO PHOTOPERIOD III. RESPONSE OF KOREAN VARIETIES

  • Ahn , Su-Bong;V.S. Vergara
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.5 no.1
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    • pp.45-49
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    • 1969
  • The photoperiod and temperature response of Korean varieties were studied under field and greenhouse conditions. Results of the experiment indicated that all varieties tested were relatively intensitive to photoperiod. The early varieties were least affected by photoperiod while the late varieties showed the greatest response. Low temperature delays flowering either under short daylength or natural daylength. In general, temperature has a greater effect than photoperiod on the growth duration of the varieties used. The late varties had longer photoperiod-sensitive phase than the early varieties. Temperature has very little effect on the photoperiod-sensitive phase. The basic vegetative phase is longer in the early varieties than the late varieties. High temperature results shorten duration of the basic vegetative phase.

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Hercynian Orogenic Cycle in the Eastern Asia (동아(東亞)에 있어서의 헤르시니아 운동(運動))

  • Son, Chi Moo
    • Economic and Environmental Geology
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    • v.4 no.2
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    • pp.59-75
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    • 1971
  • Orogenic cycle is closely related with sedimentary cycle, and a sedimentary cycle involves the birth, development and disappearance of a sedimentary basin. Paleontological studies have indicated that birth or disappearance of sedimentary basins of separated regions frequently coincided in time. In this paper, the writer presents his assumption on the East Asian analogy of the Hercynian orogenic cycle in accord to the above mentioned generalities. Previous studies, including mine have a corollary that Korea and Southern China, which had been uplifted by the Caledonian movement, changed into low-lying region with subsiding areas in the Givetian time. The writer, thus, thinks that the Hercynian orogenic cycle started in Givetian, and that the Mongolian geosyncline in China, the Gangweon Basin in Korea and the Honshu geosyncline in Japan disappeared in the Ladinian. The writer, therefore, thinks that the Ladinian marks the end of the Hercynian orogenic cycle, but this assumption will have to be rechecked in consideration with the Alpine orogenic cycle. The Late Namurian-Early Bashkirian time, the dividing period between the early and late Hercynian orogenic cycle, is thought to correspond the time of the Hongjeom Series deposition. The writer is also of the view that the Akiyoshi orogenic cycle(T. Kobayashi) corresponds the late Hercynian phase, and that the Abenian movements(M. Minato) corresponds to the range from the late Caledonian phase to the former part of late Hercynian phase.

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Spermiogenesis in the Saghalien Pygmy Shrew, Sorex minutus gracillimus (쇠뒤쥐 (Sorex minutus gracillimus)의 정자변태)

  • Heo, Jin-Chol;Lee, Jung-Hun
    • Applied Microscopy
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    • v.31 no.2
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    • pp.129-141
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    • 2001
  • To investigate the spermiogenesis of the Saghalien Pygmy shrew (Sorex minutus gracillimus), the testis obtained from mature male shrew was studied by electron microscopy, and the following results obtained based on the morphological characteristics of cell differentiation of the seminiferous epithelium in the testis. According to the fine structural differentiation, spermiogenesis of S. minutus gracillimks was divided into Golgi, cap, acrosome, maturation and spermiation phases. Beside, the Golgi and cap phases were subdivided into three steps of early, middle and late phase respectively, and acrosome phase into two steps of early and late phase , and maturation and spermiation phases has only one step respectively. Thus, the spermiogenesis of S. minutus gracillimus was divided into a total of ten steps. The chromatin granules begin to be condensed in the acrosome phase, and a perfect nucleus of sperm was formed at the spermiation phase. Mancette were appeared from the late acrosome phase to the maturation phase. The formation of sperm tail began to develop in the late Golgi phase, and completed at the spermiation phase. Multivesicular bodies were appeared from the Golgi phase to the maturation phase, recognized with pale, pale and moderate, and dense at Golgi, cap and acrosomal and matulation phases respectively.

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Fast locking single capacitor loop filter PLL with Early-late detector (Early-late 감지기를 사용한 고속 단일 커패시터 루프필터 위상고정루프)

  • Ko, Ki-Yeong;Choi, Yong-Shig
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.21 no.2
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    • pp.339-344
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    • 2017
  • A novel structure of phase locked loop (PLL) which has small size and fast locking time with Early-late detector, Duty-rate modulator, and Lock status indicator (LSI) is proposed in this paper. The area of loop filter usually occupying the larger portion of the chip is minimized using a single small capacitor. While the conventional PLL with a single capacitor loop filter cannot work stably, the proposed PLL with two charge pumps works stably because the output voltage waveform of the proposed a single capacitor loop filter is the same as the output voltage waveform of the conventional 2nd-order loop filter. The two charge pumps are controlled by the Early-late detector which detects early-late status of UP and DN signals, and Duty-rate modulator which generates a steady duty-rate signal. Fast locking time is achieved using LSI. It has been simulated and proved by HSPICE in a CMOS $0.18{\mu}m$ 1.8V process.

Growth Analysis of Silage Corn in Response to Seeding Time (파종기 이동에 따른 Silage 옥수수의 생장해석)

  • 강정훈;이호진;박병훈
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.5 no.3
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    • pp.212-219
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    • 1985
  • The field experiment was conducted to study on the growth analysis of early variety, MTC-1, and late variety, Suweon 19, in response to seeding time at Suweon. The results summarized as follows: 1. Days required for Leaf-Formation(L-phase) of MTC-1 and Suweon 19 was same but that of Node-Thickening(N-phase) was quite different, e.g. late variety has two times longer. The period of Ear-Development (E-phase) in MTC-1 was 41 days and 53 days in Suweon 19. L-and N-phase were shortened by late seeding but E-phase was not influenced by seeding time. 2. Leaf Area Index(LAI) was decreased and Leaf Area Ratio(LAR) was increased by late seeding. Days required to maximum LAR was less by late seeding. Net Assimilation Rate(NAR) of L-, N-, and E-phase was decreased but its tendency in E-phase was remarkable at late seeding. 3. Crop Growth Rate(CGR) was increased gradually in the process of plant growth and it was the highest at Husk-stage. Relative Growth Rate(RGR) was increased in L-phase and was decreased in N- and E-phase, but its tendency in N-phase was great. 4. The period required to the maximum dry matter yield was shortened by late seeding and dry matter yield was increased by early seeding.

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Significance of the AFP Level and HBsAg in Differentiation of Hepatic Masses (간종괴의 감별진단에 있어서 AFP수치와 HBsAg의 의의)

  • Kim, Jae-Woon;Park, Won-Kyu;Cho, Jae-Ho;Chang, Jae-Chun;Park, Bok-Hwan
    • Journal of Yeungnam Medical Science
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    • v.13 no.2
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    • pp.302-307
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    • 1996
  • Hepatic masses show different enhancing patterns in IV bolus computed tomography: Hepatocellular carcinoma shows high-attenuation in the early enhancing phase and low-attenuation in the late enhancing phase, hemangioma shows peripheral dot-like high-attenuation in the early enhancing phase and central high-attenuation in the late enhancing phase, and metastatic cancer and cholangiocelluar carcinoma show peripheral high-attenuation rim in the early enhancing phase and central portion gradulally high-attenuation in the late enhancing phase. but sometimes enhancing patterns of the hepatic masses are confuse. To evaluate the significance of the AFP level and HBsAg in differentiation of the hepatic masses, we retrospectively analyzed AFP level and HBsAg in 228 pathologically or radiologically confirmed hepatocellular carcinomas, and 137 pathologically nonhepatocellular cacinomas. The results were as follows : In hepatocellular carcinoma, AFP level above 20ng/ml was 77.8% and HBsAg positve was 72.6%. In nonhepatocellular carcinoma, AFP level above 20ng/ml was 3.7% and HBsAg positve was 16.1%. We concluded that AFP level and HBsAg are helpful to distinguish hepatocellular carcinoma from nonhepatocellular carcinoma, when IV bolus computed tomogram finding is uncertain.

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The Effect of Bee Venom Acupuncture into Chok-samni (ST 36) Formalin-induced Pain Behavior (족삼리(足三里) 봉독약침자극(蜂毒藥鍼刺戟)이 Formalin-induced Pain Behavior 에 미치는 영향(影響))

  • Yim, Yun-Kyoung;Seo, Jung-Chul;Kim, Yong-Suk;Kim, Chang-Hwan;Lee, Yun-Ho;Choi, Do-Young
    • Journal of Acupuncture Research
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    • v.17 no.2
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    • pp.139-152
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    • 2000
  • This study was designed to evaluate the analgesic effect of bee venom (BV) Acupuncture into different treatment points, Chok-samni (ST36) and blank loci of the gluteal muscle and back. We investigated the changes in formalin-induced pain behavior according to the pretreatment with different concentrations of BV, thirty minutes before the formalin injection. The results were summarized as follows: 1. The formalin-induced pain behavior was suppressed by pretreatment with BV into Chok-samni (ST36) in a dose dependent manner. During the early phase, 0.08mg/kg of BV showed a statistically significant suppression in the formalin-induced pain behavior. Moreover, 0.008mg/kg, 0.016mg/kg, and 0.08mg/kg of BV, except 0.0016mh/kg of BV, had significant suppresive effects on the formalin-induced pain behavior during the late phase. Therefore, these data indicated that the suppressive effect of BV acupuncture on the formalin-induced pain behavior was stronger in the late phase rather than the early phase 2. In order to investigate the analgesic effect of BV acupuncture into different treatment points, the experimental animals were divided into three groups: Chok-samni (ST36) group, gluteal group and back group. In the Chok-samni (ST36) group, the formalin-induced pain behavior during all the phases was significantly reduced as compared with that of the back group. However, as compared with that of the gluteal group, the formalin-induced pain behavior in the Chok-samni (ST36) group was decreased only in the late phase, not in the early phase. The formalin-induced pain behavior in the gluteal group was significantly reduced as compared with that of the back group in the late phase, not in the early phase. We suggested that the analgesic effect of BV acupuncture into Chok-samni (ST36) was most effective among Chok-samni (ST36), gluteal, and the back groups in formalin-induced pain behavior.

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