• Asian-Australasian Journal of Animal Sciences
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• v.30 no.12
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• pp.1796-1802
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• 2017

Objective: This experiment was conducted to evaluate the optimal space allowance on growth performance, blood profile and pork quality of growing-finishing pigs. Methods: A total of ninety crossbred pigs [$(Yorkshire{\times}Landrace){\times}Duroc$, $30.25{\pm}1.13kg$] were allocated into three treatments (0.96: four pigs/pen, $0.96m^2/pig$; 0.80: five pigs/pen, $0.80m^2/pig$; 0.69: six pigs/pen, $0.69m^2/pig$) in a randomized complete block design. Pigs were housed in balanced sex and had free access to feed in all phases for 14 weeks (growing phase I, growing phase II, finishing phase I, and finishing phase II). Results: There was no statistical difference in growing phase, but a linear decrease was observed on average daily gain (ADG, p<0.01), average daily feed intake (ADFI, p<0.01), and body weight (BW, p<0.01) with decreasing space allowance in late finishing phase. On the other hand, a quadratic effect was observed on gain to feed ratio in early finishing phase (p<0.03). Consequently, overall ADG, ADFI, and final BW linearly declined in response to decreased space allowance (p<0.01). The pH of pork had no significant difference in 1 hour after slaughter, whereas there was a linear decrease in 24 h after slaughter with decreasing space allowance. Floor area allowance did not affect pork colors, but shear force linearly increased as floor space decreased (p<0.01). There was a linear increase in serum cortisol concentration on 14 week (p<0.05) with decreased space allocation. Serum IgG was linearly ameliorated as space allowance increased on 10 week (p<0.05) and 14 week (p<0.01). Conclusion: Data from current study indicated that stress derived from reduced space allowance deteriorates the immune system as well as growth performance of pigs, resulting in poor pork quality. Recommended adequate space allowance in a grow-to-finish production system is more than $0.80m^2/pig$ for maximizing growth performance and production efficiency.

• Proceedings of the Korean Aquaculture Society Conference
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• 2003.10a
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• pp.76-77
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• 2003

Olive flounder Paralichthys olivaceus larvae and early juveniles were reared for 43 days after hatching in order to observe the effects of starvation during development and metamorphosis. Morphological, histological and biochemical measurements were made to assess the nutritional condition during growth and starvation from pre-matamorphic through post-metamorphic phases. Two groups of fish were compared ; one with sufficient food supply and one under continuous starvation until death. Among morphometric analyses, both ratios of body height at anus/head height and pre-/post-anal lengths appeared to be sensitive to starvation during which substantial reduction was observed within a day of food deprivation. Histological variables as intestinal and rectal epithelial heights and gall bladder volume changed significantly with onset of starvation. The gut epithelial heights of starving fish decreased with advances in starvation, although they fluctuated during mid-metamorphic phase. In contrast, gall bladder volume increased remarkably soon after starvation. Ontogenetic changes in both gut epithelial height and gall bladder volume were evident, those associated with settlement and/or completion of metamorphosis. Abrupt decrease in the RNA/DNA ratios of starving fish were found right after onset of starvation. Even in the fed fish marked fluctuations in its ratios during metamorphosis were observed, evident by decreasing from late-metamorphic to post-metamorphic stages. These findings suggest that a combination of morphologically and histologically sensitive characteristics, and biochemical measurement could be utilized as a measure to evaluate nutritional condition related to starvation in wild olive flounder larvae and juveniles.

• Journal of Microbiology and Biotechnology
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• v.21 no.1
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• pp.100-108
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• 2011

A method for the rapid detection and quantification of Newcastle disease virus (NDV) produced in an animal cell culture-based production system was developed to enhance the speed of the NDV vaccine manufacturing process. A SYBR Green I-based real-time RT-PCR was designed with a conventional, inexpensive RT-PCR kit targeting the F gene of the NDV LaSota strain. The method developed in this study was validated for specificity, accuracy, precision, linearity, limit of detection (LOD), limit of quantification (LOQ), and robustness. The validation results satisfied the predetermined acceptance criteria. The validated method was used to quantify virus samples produced in an animal cell culture-based production system. The method was able to quantify the NDV samples from mid- or late-production phases, but not effective on samples from the early-production phase. For comparison with other quantifiable methods, immunoblotting, plaque assay, and tissue culture infectious dose 50 ($TCID_{50}$) assay were also performed with the NDV samples. The results demonstrated that the real-time RT-PCR method is suitable for the rapid quantification of virus particles produced in an animal cell-culture-based production system irrespective of viral infectivity.

• 한국신재생에너지학회:학술대회논문집
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• 2010.11a
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• pp.59.2-59.2
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• 2010

Throughout history, daylight has been a primary source of lighting in buildings, supplemented originally with burned fuels and more recently with electrical energy. Before daylight was supplemented or replaced with electric light in the late 19th-century, consideration of good daylight strategies was essential. As we entered the mid-20th-century, electric light supplanted daylight in buildings in many cases. Fortunately, during the last quarter of the 20th-century and early years of this century, architects and designers have recognized the importance and value of introducing natural light into buildings. There are many simple strategies that can enhance daylighting and reduce the need for electric lights. Good quality daylight is always welcome, but remember that the electric lights must be dimmed or shut off in order for daylighting to save energy. We designed and built mirror systems and vertical daylighting devices to improve daylight condition of office buildings in bad condition because urban density is getting higher. This case study aims to analysis the principles and characteristics of mirror systems and vertical daylighting devices and selected the method that can improve constructability. The results of this study are going to use the back data to set-up the design standards. Hereafter we're going to progress the performance test and product the design manual to improve applicability of daylighting systems at design phase.

• Korean Journal of Animal Reproduction
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• v.19 no.2
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• pp.105-117
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• 1995

This study was carried out to investigate the histomorphological changes and the electrophoretic patterns of egg components, obtained from 100 of 1-year-old female catfish(Silurus asotus). Especially, the light microscopic and ultrastructural changes of ooplasm and follicular membranes of oocytes, were observed by light and transmission electron microscope. All data were collected from October in 1992 to May in 1993. The size of the nucleoli and number of the yolk granules increased as the oocyte grew. Yolk granules were deposited in the oocyte as fluid. Due to the presence of large early and late maturing oocytes, their ovaries were large, transparent, granular, and greenish in color. As the percentages of fish in LMO and RO stage increased from March to April, mean of GSI values(19.95%) increased. Follicle cells such as granulosa cell and thecal cell change a squamous into cuboid shape in LPO and EMO stage. Processes, microvilli, from the granulosa cells and from the oocyte grow and make contact with each other in the pore canals of the zona radiata during vitellogenesis, but are withdrawn as the zona radiate becomes more compact and devoid of pore canals during oocyte maturation. The electrophoretic pattern of major band in mature stage was much thicker(21k, 24k, 32k, 45k, 67∼110k, 170k dalton) than that in previtellogenic phase.

• Economic and Environmental Geology
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• v.27 no.3
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• pp.247-261
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• 1994

Honam Shear Zone is a mylonite zone approximately parallel to the NE-SW trend located southern part of Korea peninsula. Geologic ages and petrogenesis of foliated granites in this zone are as follows: Igneous rocks of this zone are composed of granite gneiss, Paleozoic granites, Songrim granites, Jurassic granites and Cretaceous granites. Foliated granites show deformed phase of Paleozoic and Songrim granites during Daebo Orogeny. And isotopic ages obtained from foliated granites are early Permian to late Triassic period (276~200 Ma). Most of foliated granite masses are igneous complex consisting of a series of differential product of cogenetic magma. The individual rock mass of foliated granites plotted on Harker diagram shows mostly similar trend of calc-alkali series. REE diagram indicates that LREE amount of foliated granites are more enriched than HREE and negative Eu anomalies of them are weaker than those of the other granites. From these data, we suggest the rocks are generated from continental margin under syntectonic environment. Original magma type of foliated granites correspond to I-type, syn-collision type and Hercyano type. In compressive stress field between Ogcheon folded belt and Youngnam massif, foliated granites had formed due to mylonitic deformation. Those facts indicate that magma of foliated granites would had been generated by melting in lower crust or contamination in upper mantle.

• Transactions of the Korean Society of Automotive Engineers
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• v.17 no.4
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• pp.93-100
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• 2009

Intake/exhaust valve timing and exhaust cam lift were changed to control the internal exhaust gas recirculation (IEGR) and combustion phase of homogeneous charge compression ignition (HCCI) engine. To measure the IEGR rate, in-cylinder gas was sampled during from intake valve close to before ignition start. The lower exhaust cam made shorter valve event than higher exhaust cam and made IEGR increase because of trapping the exhaust gas. IEGR rate was more affected by exhaust valve timing than intake valve timing and increased as exhaust valve timing advanced. In-cylinder pressure was increased near top dead center due to early close of exhaust valve. Ignition timing was more affected by intake valve timing than exhaust valve timing in case of exhaust valve lift 8.4 mm, while ignition timing was affected by both intake and exhaust valve timing in case of exhaust valve 2.5 mm. Burn duration with exhaust valve lift 2.5 mm was longer than other case due to higher IEGR rate. The fuel conversion efficiency with higher exhaust valve lift was higher than that with lower exhaust valve lift. The late exhaust and intake maximum open point (MOP) made the fuel conversion efficiency improve.

• Asian Pacific Journal of Cancer Prevention
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• v.14 no.11
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• pp.6653-6656
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• 2013

Background: The single most common proto-oncogene change in human neoplasms is a point mutation in RAS genes. A wide range of variation in frequency of KRAS mutations has been seen in hematologic malignancies. Despite this, RAS roles in leukemogenesis remain unclear. The frequency of KRAS mutations in CML has been reported to be between zero an 10%. Many attempts have been done to develop an anti-RAS drug as a therapeutic target. Materials and Methods: This cross sectional study was performed in Mashhad University of Medical Sciences, Mashhad, Iran from 2010-2012. In 78 CML patients (diagnosed according to WHO 2008 criteria) in chronic or accelerated phases, KRAS mutations in codons 12 and 13 were analyzed using a modified PCR-restriction fragment length polymorphism (RFLP) method. Results: We did not detect any KRAS mutations in this study. Conclusions: KRAS mutations are overall rare in early phase CML and might be secondary events happening late in leukemogenesis cooperating with initial genetic lesions.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.87-87
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• 2017

Flowering time of either early or late is one of the crucial parameters that determine the crop productivity. Therefore, elucidation of regulatory mechanisms of flowering time should contribute to breeding for yield enhancement. However, comprehensive explanation on molecular mechanism of flowering has not yet been reported in hexaploidy common wheat (Triticum asetivum L.). The mechanism of flowering in wheat has been studied mostly using flag leaf or floral meristem. The exposed peduncle, which is a shoot part between bottom of the spike and flag leaf, could be an important tissue that is responsible for flowering through various molecules expressing. To clarify for transcriptomic dynamics in the wheat peduncle that was uncovered by leaf sheath of flag leaf, RNA sequencing and transcriptomic analysis were conducted. With this, we also analyzed other transcriptomic results deposited in the public DB to identify genes specially expressed in peduncle tissue at transition from vegetative to reproductive phase. The obtained results will provide valuable information to understand the role of peduncle for flowing regulation in wheat aimming for elucidation of the regulatory mechanism of wheat flowering.

• Korean Journal of Veterinary Research
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• v.34 no.4
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• pp.867-872
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• 1994

Progesterone catabolizing enzyme, the enzyme $20{\alpha}$-hydroxysteroid dehydrgenase($20{\alpha}$-HSD) is pivotal in the regulation of ovarian function in rodents, which catabolizes progesterone into biologically inactive $20{\alpha}$-hydroxypregn-4-en-3-one($20{\alpha}$-OHP). In this study was carried out the influence of $20{\alpha}$-HSD activity of ovarian function, we investigated changes in ovarian cytosol $20{\alpha}$-HSD activity and serume progesterone concentration during the estrous cycles and pregnancy in rat. During the estrous cycles, the $20{\alpha}$-HSD activities were highest on the progestrous, but serum progesterone concentration was lowest on this phase. During the pregnancy, the $20{\alpha}$-HSD activities were relatively higher early pregnancy(day-1-3 gestation) and late pregnancy(day 21 to parturition), serum progesterone concentration was maintained significantly high to day 19 of gestation. The $20{\alpha}$-HSD activities were lower during the middle pregnancy. From these results, ovarian $20{\alpha}$-HSD activities may possibly act as physiologically very important in the control and maintenance of estrous cycles in rat.