• Title/Summary/Keyword: early stage

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Porcine Oocytes with Meiotic Competence are Synchronized Early Stage of Germinal Vesicle (GV)

  • Park, Kwang-Wook
    • Reproductive and Developmental Biology
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    • v.34 no.3
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    • pp.169-173
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    • 2010
  • Correlations between cumulus cells and germinal vesicle (GV) chromatin configuration were examined in porcine oocytes. Cumulus-oocyte complexes (COCs) were collected from 2~6 mm follicles and divided into three categories according to cumulus cell morphology. "A" group was compacted COCs with more than three cumulus cell layers. "B" group was COCs with less cumulus cell layers than "A" group. "C" group was COCs with one or less layer of cumulus cells. Cumulus cells were removed 0.1% hyaluronidase, and denuded oocytes were stained with Hoechst 33342. GV chromatin configuration was classified into GV-Con and GV-Dis. GV-Con meant that a nucleus was surrounded by condensed chromatin in a ring. GV-Dis meant that filamentous chromatin clumps were distributed in nucleus. The proportion (80.2%) of GV-Con in "A" group was significantly higher than "B" (62.0%) or "C" (44.9%). The proportion (55.1%) of GV-Dis in "C" group was significantly higher than "A" (19.8%) or "B" (38.0%). The meiotic competence of COCs was examined after 44 h culture. The proportion (90.0%) of oocytes reaching to metaphase II (M-II) in "A" group was significantly higher than "B" (76.5%) or "C" (45.5%). In conclusion, oocytes with good quality cumulus cell layers are synchronized early GV stage, and early GV stage is important for meiotic competence in pigs.

The Marital Relationship Enhancement Program and Its Effects for Couples in the Early Marriage Stage (결혼초기 부부관계향상 프로그램의 효과검증 -PREP(Prevention and Relationship Enhancement Program)을 중심으로-)

  • Yu Eun-Hee;Kim Deuk-Sung
    • Journal of Families and Better Life
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    • v.23 no.1 s.73
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    • pp.1-18
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    • 2005
  • The purpose of this study was to construct the Marital Relationship Enhancement Program based on PREP(Prevention and Relationship Enhancement Program), and to examine the effect of it for couples in the early marriage stage. To perform this program, six session of time-limited(two hour) were carried out experimental group. and subjects were assigned to experimental group(N=6 couples) and control group(N=6 couples). Scales of PREPARE II (PREmarital Personal And Relationship Evaluation), Couple Communication Scales and Commitment Scales were used as pre-post-follow instruments of this study. Major finding were as follows : 1. Marital Relationship Enhancement Program for couples in the early marriage stage has meaningful consequences for the improvement of participants' couple relationship. 2. Marital Relationship Enhancement Program had meaningful consequence for the improvement of participants' realistic expectation of marriage, communication and conflict resolution skills and leisure activities. These positive effects maintained at follow-up. However, it hadn't meaningful consequence for the improvement of participants' marital commitment. 3. Marital Relationship Enhancement Program had also meaningful consequence for the improvement of the wives' realistic expectation of marriage, communication and conflict resolution skills and leisure activities, of the husbands' communication and conflict resolution skills.

Case Study on Public Support for Design Management in UK;Focusing on Early-stage Enterprises (벤처기업의 디자인 경영 지원체계와 그 성공사례;영국을 중심으로)

  • Kim, Do-Hyeon;Yi, Jae-Kyung
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.2 no.3
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    • pp.1-32
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    • 2007
  • The term of design management has become more and more widely used. It reflects the expectation, both practical and academic, that the combination of design and management expertises will open a new chapter of management. This study is to review current situation and related annecdotes on design management in UK where "design management" is first coined and became government agenda. It reviews the definition and understanding of design management by policy makers, and the use of various measures to enhance design management capabilities of UK firms. The focus of the study is early-stage enterprises, in which the success is much dependent on the innovative capability believed to be linked with design management capability.

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Effect of Chuna Treatment(Manipulation) on Cervical Sprain caused by Traffic Accident in Early Stage. - by Analysis of the Heart Rate Variability(HRV) and Visual Analogue Scale(VAS) - (경추 추나 치료가 교통사고 환자의 초기 HRV, VAS 변화에 미치는 영향)

  • Park, Ji-Hyun;Lee, Jung-Min;Hong, Seo-Young
    • The Journal of Churna Manual Medicine for Spine and Nerves
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    • v.4 no.2
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    • pp.47-60
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    • 2009
  • Objectives : The purpose of this study is to investigate the effect of chuna treatment on cervical sprain caused by traffic accident in early stage. Methods : This study carried out on 20 patients who have received hospital treatment in Daejeon Univ. cheonan Oriental Hospital. Non-chuna group got acupunture-therapy, herbal medication, physical therapy and Chuna group got chuna treatment besides. We measured Heart Rate Variability(HRV) and Visual Analog Scale(VAS) on 2nd, 4th day. Results : After being treated by our methods, Chuna Group showed the inclination to balance the sympathetic and parasympathetic nerve. In chuna group, an autonomic nerve activity showed the inclination to increase. But there were no significant difference between both groups. Chuna group's VAS were significantly decreased(p=0.043). Conclusions : The results suggest that Chuna treatment help traffic accident patients in early stage to reduce pain. Refer to autonomic nerve system, chuna treatment seem to do positive effect but Further long tenn study in a large scale is needed.

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Microarray Analysis of Genes Involved with Shell Strength in Layer Shell Gland at the Early Stage of Active Calcification

  • Liu, Zhangguo;Zheng, Qi;Zhang, Xueyu;Lu, Lizhi
    • Asian-Australasian Journal of Animal Sciences
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    • v.26 no.5
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    • pp.609-624
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    • 2013
  • The objective of this study was to get a comprehensive understanding of how genes in chicken shell gland modulate eggshell strength at the early stage of active calcification. Four 32-week old of purebred Xianju hens with consistent high or low shell breakage strength were grouped into two pairs. Using Affymetrix Chicken Array, a whole-transcriptome analysis was performed on hen's shell gland at 9 h post oviposition. Gene ontology enrichment analysis for differentially expressed (DE) transcripts was performed using the web-based GOEAST, and the validation of DE-transcripts was tested by qRT-PCR. 1,195 DE-transcripts, corresponding to 941 unique genes were identified in hens with strong eggshell compared to weak shell hens. According to gene ontology annotations, there are 77 DE-transcripts encoding ion transporters and secreted extracellular matrix proteins, and at least 26 DE-transcripts related to carbohydrate metabolism or post-translation glycosylation modification; furthermore, there are 88 signaling DE-transcripts. GO term enrichment analysis suggests that some DE-transcripts mediate reproductive hormones or neurotransmitters to affect eggshell quality through a complex suite of biophysical processes. These results reveal some candidate genes involved with eggshell strength at the early stage of active calcification which may facilitate our understanding of regulating mechanisms of eggshell quality.

Effects of Substrate Sizes and Organic Contents on Larval Settlement and Growth in the Early Stage of the Polychaete Marphysa sanguinea (바위털갯지렁이(Marphysa sanguinea) 유생의 착저와 초기 성장에 미치는 기질 크기 및 유기물 함량)

  • Phoo, War War;Kim, Sung Kyun;Kim, Chang-Hoon
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.53 no.1
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    • pp.132-138
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    • 2020
  • Marphysa sanguinea is the most well-known polychaete species with a high economic value. However, this species has a high mortality in the early rearing stage of aquaculture. This study was conducted to find out the optimal substrate size and organic contents for the growth and survival rate of M. sanguinea larvae and juveniles. It was observed that the smaller grain size (<Ø 0.063 mm) and high organic contents (5-10%) induced settlement and reduced settlement time of larvae. Moreover, the growth and survival rate of larvae reached high levels at Ø 0.004-0.016 mm of grain sizes and 5-7.5% of organic contents as advantageous substrates for settlement. The survival rate of juveniles reached over 90% in less than Ø 0.016 mm substrate on 15-day experiment for different grain sizes of substrates. These results indicated that substrate compositions of less than Ø 0.016 mm of sand size and 5-7.5% of organic contents in mud will enhance the productivity of M. sanguinea at the early stage.

A transcode scheduling technique to reduce early-stage delay time in playing multimedia in mobile terminals (이동 단말기에서 멀티미디어 연출시 최초 재생 지연시간을 줄이기 위한 트랜스코드 스케줄링 기법)

  • Hong, Maria;Yoon, Joon-Sung;Lim, Young-Hwan
    • The KIPS Transactions:PartB
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    • v.10B no.6
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    • pp.695-704
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    • 2003
  • This paper proposes a new scheduling technique to play multimedia data streams in mobile terminals. The paper explores the characteristics of multimedia data streams , firstly. On basis of these characteristics, selection of specific data stream can be possible as well as transcoding protest. Our approach aims at reducing the early-stage delay time more effectively since it makes possible to select and transcodes some specific streams by employing a selection policy rather than transcoding all streams in the playing process Thus, this paper suggests a stream selection policy for the transcoding based on EPOB (End Point of Over Bandwidth). It aims to lower the required bandwidth of multimedia streams than the network bandwidth level and also to minimize early-stage delay time for multimedia streams, which is to be played in mobile terminals.

Design for Vibration of Radiator at the Early Stage of Product Development Process in Construction Equipment (건설중장비 제품개발 초기 단계에서의 라디에이터 진동 설계)

  • Kang, Hyun-Seok;Kim, Sung-Hwan;Kang, Jong-Min
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2011.10a
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    • pp.271-276
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    • 2011
  • The working conditions of construction equipments such as excavators, wheel loaders and haulers are very tough and severe in fact. To design main components of construction equipment under the severe environment, it's important for engineers to consider design for vibration durability point of view at the early stage of product development process. Radiator as a cooling unit of construction equipment is one of critical components to apply design for vibration. We present a design for vibration process and methodology on the radiator system in construction equipment industries. From the natural frequency and the random vibration analysis based on field vibration test data, we could find current status of radiator layout design to develop and made best layout specification of radiator design to decide for product development process at the early stage.

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Effect of Aging Treatment on the Mechanical Properties and Damping Capacity of 12Cr Heat Resistant Steel with Ferrite Phase (페라이트 상을 갖는 12Cr 내열강의 기계적성질 및 감쇠능에 미치는 시효처리의 영향)

  • Kang, C.Y.;Choi, H.G.;Park, H.K.;Sung, J.H.;Lee, D.H.
    • Journal of the Korean Society for Heat Treatment
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    • v.24 no.1
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    • pp.23-30
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    • 2011
  • This study was carried out to investigate the effect of aging treatment on the mechanical properties and damping capacity of 12Cr heat resistant steel with ferrite phase. While hardness values in ferrite phase was not changed, that in martensite phase was dramatically dropped in early stage of aging treatment and then gradually decreased with increase of aging time. As aging treatment was carried out, the precipitation was not detected in ferrite phase, while carbides were precipitated in martensite phase. With increasing the aging time, tensile strength eventually decreased while impact toughness increased rapidly in the early stage of aging and then gradually increased. Besides, it was confirmed that damping capacity was not changed in the early stage of aging and then gradually increased with increase of aging time.

Effects of Hexoses on In Vitro Development of Parthenogenetic Embryos in the Pigs (돼지 단위발생란의 체외발육시 6탄당의 영향)

  • Yoon S. Y.;Kim C. I.;Cheong H. T.;Yang B. K.;Park C. K.
    • Journal of Embryo Transfer
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    • v.20 no.2
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    • pp.113-121
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    • 2005
  • This study was conducted to investigate the effects of different hexoses (glucose, mannose, galactose and fructose) on in vitro development of parthenogenetic embryos in the pigs. When the parthnogenetic embryos were cultured in medium with concentrations of 5mM glucose or 1mM galactose, the rates of embyos developed to morula and blastocyst stages were significantly higher than those in another culture conditions (P<0.05). However, high concentration of galactose inhibited development to morula and blastocyst stages. Addition of hexoses at early stage of porcine parthenogenetic embryos were effective for in vitro development. Especially, the embryos cultured in medium with glucose at early stage were effective for development to 2-cell $(72\%)$ and blastocyst $(19\%)$ stages compared with embryo cultured without glucose. From the present results, it is suggested that development of porcine parthenogenetic embryos can improve in medium with 5mM glucose. The concentration of 1mM galactose was also effective for development of porcine parthenogenetic embryos. It also show that parthenogenetic embryos cultured with glucose at early stage can improve in vitro development.