• 한국작물학회:학술대회논문집
• /
• 한국작물학회 2017년도 9th Asian Crop Science Association conference
• /
• pp.103-103
• /
• 2017

Vigorous root growth at the seedling stage in dry direct-seeded conditions is considered as a critical trait because it is involved in seedling emergence, early vegetative vigour, nutrient uptake as well as drought tolerance. In this study, we performed QTL mapping using the recombinant inbred lines obtained from the cross between Tongil-type Dasan and temperate japonica TR22183 (DT-RILs) to identify QTL underlying early root development. TR22183, which was previously reported to have high nitrogen utility and cold tolerance, showed vigorous root growth at the seedling stage in semi-drought conditions. Root length, fresh weight and dry weight of TR22183 were significantly higher than in Dasan. By QTL analysis with genotyping-by-sequencing method, we identified two QTLs for root fresh weight (RFW) in chromosome 7 and root dry weight (RDW) in chromosome 8, explaining phenotypic variances of 13.5% and 10.6%, respectively. These QTLs would be used to develop rice varieties adapted to direct-seeded cultivating system.

• 한국응용약물학회:학술대회논문집
• /
• 한국응용약물학회 1994년도 제2회 추계심포지움
• /
• pp.135-140
• /
• 1994

Over past decades, numerous in vitro model has been developed to investigate drug metabolism. In the order of complexity we found the isolated perfused liver, hepatocytes in co-culture with epithelial cells, hepatocytes in suspension and in primary culture and subcellular hepatic microsomal fractions. Because they can be easily prepared from both animals (pharmacological and toxicological species) and humans (whole livers as well as biopsies obtained during surgery) hepatocytes in primary culture provide the most powerful model to better elucidate drug behavior at an early stage of preclinical development such as : 1. the characterization of main biotransformation reactions. 2. the identification of phase I and phase II isozymes involved in such reactions 3. the evaluation of interspecies differences allowing the selection of a second toxicological animal species more closely related to man on the basis of metabolic profiles 4. the detection of the inducing and/or inhibitory effects of a drug on metabolic enzymes, the prediction of drug interactions 5. the estimation of inter-individual variability in biotransformation reactions. The use of hepatocytes, and in particular those obstained from humans, at an early stage of drug development allows the obtention of more predictive preclinical data and a better knowledge of drug behavior in humans before the first administration of the drug in healthy volunteers.

• 말소리와 음성과학
• /
• 제6권4호
• /
• pp.161-169
• /
• 2014

This study investigated changes in the prelinguistic vocal production of typically developing infants aged 5-20 months based on Stark Assessment of Early Vocal Development-Revised (SAEVD-R). Fifty-eight typically developing infants participated in the study, and they were divided into four age groups, 5-8 months, 9-12 months, 13-16 months, and 17-20 months of age. Vocalization samples were collected from infants' play activities and were classified into 5 levels and 23 types using SAEVD-R. The results revealed that the four age groups showed significant differences in production proportion of vocalization levels. Level 1, 2, 4, and 5 vocalizations exhibited significantly different across the four age groups. Level 3 was predominantly produced across every age group. Therefore, the vocalization level was not significantly different across the four age groups. Especially, vowels in Level 3 vocalization predominantly produced across all ages during a long period. Also, significant increases in the proportion of Levels 4 and 5 occurred after 9 months, which suggested that the production of cannonical syllables is a key indicator of advancement in prelinguistic vocal development. The results have clinical implication in early identification and speech-language intervention for young children with speech delays or at risk.

• BMB Reports
• /
• 제41권10호
• /
• pp.685-692
• /
• 2008

Colorectal cancer (CRC) is the third most common malignancy in the world. Because CRC develops slowly from removable precancerous lesions, detection of the disease at an early stage during regular health examinations can reduce both the incidence and mortality of the disease. Although sigmoidoscopy offers significant improvements in the detection rate of CRC, its diagnostic value is limited by its high costs and inconvenience. Therefore, there is a compelling need for the identification of noninvasive biomarkers that can enable earlier detection of CRC. Accordingly, many validation studies have been conducted to evaluate genetic, epigenetic or protein markers that can be detected in the stool or in serum. Currently, the fecal-occult blood test is the most widely used method of screening for CRC. However, advances in genomics and proteomics combined with developments in other relevant fields will lead to the discovery of novel non invasive biomarkers whose usefulness will be tested in larger validation studies. Here, non-invasive molecular biomarkers that are currently used in clinical settings and have the potential for use as CRC biomarkers are discussed.

• BMB Reports
• /
• 제41권9호
• /
• pp.626-634
• /
• 2008

Novel cancer biomarkers are required to achieve early diagnosis and optimized therapy for individual patients. Cancer is a disease of the genome, and tumor tissues are a rich source of cancer biomarkers as they contain the functional translation of the genome, namely the proteome. Investigation of the tumor tissue proteome allows the identification of proteomic signatures corresponding to clinico-pathological parameters, and individual proteins in such signatures will be good biomarker candidates. Tumor tissues are also a rich source for plasma biomarkers, because proteins released from tumor tissues may be more cancer specific than those from non-tumor cells. Two-dimensional difference gel electrophoresis (2D-DIGE) with novel ultra high sensitive fluorescent dyes (CyDye DIGE Fluor satulation dye) enables the efficient protein expression profiling of laser-microdissected tissue samples. The combined use of laser microdissection allows accurate proteomic profiling of specific cells in tumor tissues. To develop clinical applications using the identified biomarkers, collaboration between research scientists, clinicians and diagnostic companies is essential, particularly in the early phases of the biomarker development projects. The proteomics modalities currently available have the potential to lead to the development of clinical applications, and channeling the wealth of produced information towards concrete and specific clinical purposes is urgent.

• 한국정밀공학회지
• /
• 제22권10호
• /
• pp.80-89
• /
• 2005

In this paper, time-frequency analysis using wavelet packet transform and advanced-MDSA (Multiple Dimensional Spectral Analysis) which based on wavelet packet transform is applied fur fault source identification and diagnosis of early detection of fault non-stationary sound/vibration signals. This method is analyzing the signal in the plane of instantaneous time and instantaneous frequency. The results of ordinary coherence function, which obtained by wavelet packet analysis, showed the possibility of early fault detection by analysis at the instantaneous time. So, by checking the coherence function trend, it is possible to detect which signal contains the major fault signal and to know how much the system is damaged. Finally, It is impossible to monitor the system is damaged or undamaged by using conventional method, because crest factor is almost constant under the range of magnitude of fault signal as its approach to normal signal. However instantaneous coherence function showed that a little change of fault signal is possible to monitor the system condition. And it is possible to predict the maintenance time by condition based maintenance for any stationary or non-stationary signals.

• 대한수의학회지
• /
• 제33권2호
• /
• pp.241-247
• /
• 1993

Forty day old piglets were intranasally inoculated with 2ml of Aujeszky's disease virus (NYJ-1-87 strain, $10^{7.0}$ $TCID_{50/0.2}ml$), and the viral antigens were detected in nasal and circulating white blood cells for 20 days after inoculation by immunocytochemical method. Antibody titers in the blood were also detected by neutralizing test and Aujeszky's disease serodiagnostic kit(Choong Ang) in this periods. 1. Viral antigens were detected by the immunocytochemical technigue, and positive reactions were observated in nasal cells from the 2nd to the l0th days after inoculation and circulated white blood cells from the 4th to the 12th days after inoculation. 2. In neutralization test antibodies levels showed titers of 2 on the 8th day, 8 on the l5th day, 16 on the 18th day and 32 on the 20th day after inoculation. In serodiagnostic kit test positive reactions were observed after the 15th day after inoculation.

• The Korean Journal of Ecology
• /
• 제22권1호
• /
• pp.45-50
• /
• 1999

김치 발효 초기에 bacteriocin 생성 유산균을 분리하고자 하였다. 분리 유산균은 형태, 배양 및 생리학적인 특징과 16S rDNA의 부분적인 염기서열로부터 Lactococcus lactis로 동정되었다. 분리균주가 생성한 bacteriocin은 Listeria monocytogenes, Staphylococcus aureus와 같은 그람 양성 병원성 세균과 몇몇 유산균에 대해 항균활성이 있었고, 그람음성균인 Yesinia에는 활성이 없었다. bacteriocin의 활성은 protease, protease ⅩⅣ, a-chymotrypsin과 pepsin에 대해서 활성이 소실되었으나, lipase, trypsin, lysozyme에 대해서는 활성이 유지되었다. bacteriocin의 활성은 pH 2∼11에서 안정적이며 l00℃에서 10분간 열처리시에도 변하지 않았다. 따라서 L. monocytogenes는 발효초기에 L. lactis에 의하여 억제될 수 있다.

• Journal of Preventive Medicine and Public Health
• /
• 제42권6호
• /
• pp.349-355
• /
• 2009

Biomarkers are characteristic biological properties that can be detected and measured in a variety of biological matrices in the human body, including the blood and tissue, to give an indication of whether there is a threat of disease, if a disease already exists, or how such a disease may develop in an individual case. Along the continuum from exposure to clinical disease and progression, exposure, internal dose, biologically effective dose, early biological effect, altered structure and/or function, clinical disease, and disease progression can potentially be observed and quantified using biomarkers. While the traditional discovery of biomarkers has been a slow process, the advent of molecular and genomic medicine has resulted in explosive growth in the discovery of new biomarkers. In this review, issues in evaluating biomarkers will be discussed and the biomarkers of environmental exposure, early biologic effect, and susceptibility identified and validated in epidemiological studies will be summarized. The spectrum of genomic approaches currently used to identify and apply biomarkers and strategies to validate genomic biomarkers will also be discussed.

• 한국의사학회지
• /
• 제26권1호
• /
• pp.27-39
• /
• 2013

The Theory of Meridian can be classified into early form of pre- and present form of after-. The representative early form of meridian is Eleven Meridians in Mawangdui scrolls(馬王堆帛書) which use Six Qi Diagnosis(六氣辨證). because it did not adopt Visceral Pattern Identification(臟腑辨證). The present form of meridian is Twelve Meridians of . It has developed to investigate relations between Visceral and disease with Five Phase Theory. Analyzing the name of the Six Meridians in the Shanghanlun(傷寒論)On Cold Damage, there are no hands, feet and visceral signs. and it is almost identical to Eleven Meridians in Mawangdui scrolls. According to , Eleven Meridians use only Six Foot Meridians(足六經) to classify diseases. This fact was applied identically to the Six Meridians in the Shanghanlun. Therefore many doctors, especially Hanzhihe(韓祗和) and Zhangzhicong(張志聰) mentioned that "I have never seen a book on Shanghanlun discuss about Hand Meridians(手經)."