• Asian-Australasian Journal of Animal Sciences
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• v.25 no.11
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• pp.1540-1545
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• 2012

Steroidogenesis requires coordination of the anabolic and catabolic pathways of lipid metabolism, but the profile of proteins associated with progesterone synthesis in cyclic and pregnant corpus luteum (CL) is not well-known in cattle. In Experiment 1, plasma progesterone level was monitored in cyclic cows (n = 5) and pregnant cows (n = 6; until d-90). A significant decline in the plasma progesterone level occurred at d-19 of cyclic cows. Progesterone level in abbatoir-derived luteal tissues was also determined at d 1 to 5, 6 to 13 and 14 to 20 of cyclic cows, and d-60 and -90 of pregnant cows (n = 5 each). Progesterone level in d-60 CL was not different from those in d 6 to 13 CL and d-90 CL, although the difference between d 6 to 13 and d-90 was significant. In Experiment 2, protein expression pattern in CL at d-90 (n = 4) was compared with that in CL of cyclic cows at d 6 to 13 (n = 5). Significant changes in the level of protein expression were detected in 32 protein spots by two-dimensional polyacrylamide gel electrophoresis (2-DE), and 23 of them were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Six proteins were found only in pregnant CL, while the other 17 proteins were found only in cyclic CL. Among the above 6 proteins, vimentin which is involved in the regulation of post-implantation development was included. Thus, the protein expression pattern in CL was disorientated from cyclic luteal phase to mid pregnancy, and alterations in specific CL protein expression may contribute to the maintenance of pregnancy in Korean native cows.

• Journal of Mushroom
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• v.15 no.1
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• pp.14-20
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• 2017

The oyster mushroom cultivation house typically has multiple layers of growing shelves that cause the disturbance of air circulation inside the mushroom house. Due to this instability in the internal environment, growth distinction occurs according to the area of the growing shelves. It is known that minimal air circulation around the mushroom cap facilitates the metabolism of mushrooms and improves their quality. For the purpose of this study, a CFD analysis FLUENT R16 has been carried out to improve the internal environment uniformity of the oyster mushroom cultivation house. It is found that installing a section of the working passage towards the ceiling is to maintain the internal environment uniformity of the oyster mushroom cultivation house. When all the environment control equipment - including a unit cooler, an inlet fan, an outlet fan, an air circulation fan, and a humidifier - were operated simultaneously, the reported Root Mean Square (RMS) valuation the growing shelves were as follows: velocity 23.86%, temperature 6.08%, and humidity 2.72%. However, when only a unit cooler and an air circulation fan operated, improved RMS values on the growing shelves were reported as follows: velocity 23.54%, temperature 0.51%, and humidity 0.41%. Therefore, in order to maintain the internal environment uniformity of the mushroom cultivation house, it is essential to reduce the overall operating time of the inlet fan, outlet fan, and humidifier, while simultaneously appropriately manage the internal environment by using a unit cooler and an air circulation fan.

• Korean Journal of Materials Research
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• v.12 no.12
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• pp.962-966
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• 2002

The $SrBi_2$$Nb_2$$O_{9}$ (SBN) thin films were deposited with $SrNb_2$$O_{6}$ / (SNO) and $Bi_2$$O_3$ targets by co-sputtering method. For the growth of SBN thin films, we adopted the various power ratios of two targets; the power ratios of the SNO target to $Bi_2$$O_3$ target were 100 W : 20 W, 100 W : 25 W, and 100 W : 30 W during sputtering the SBN films. We found that the electrical properties of SBN films were greatly dependent on Bi content in films. The $Bi_2$Pt and $Bi_2$$O_3$ phase as second phases occurred at the films with excess Bi content greater than 2.4, resulting in poor ferroelectric properties. The best growth condition of the SBN films was obtained at the power ratio of 100 W : 25 W for the two targets. At this condition, the crystallinity and electrical properties of the films were improved at even low annealing temperature as $700^{\circ}C$ for 1h in oxygen ambient and the Sr, Bi and Nb component in the SBN films were about 0.9, 2.4, and 1.8 respectively. From the P-E and I-V curves for the specimen, the remnant polarization value ($2P_{r}$) of the SBN films was obtained about 6 $\mu$C/c $m^2$ at 250 kV/cm and the leakage current density of this thin film was $2.45$\times$10^{-7}$ $A/cm^2$ at an applied voltage of 3 V.V.

• Journal of The Korean Society of Grassland and Forage Science
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• v.7 no.1
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• pp.25-30
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• 1987

There are differences in opinion as to whether nitrogen fertilizer should be used when establishing alfalfa (Medicago sativa L.). Various reports show that under a hot environment, rhizobia (Rhizobium meliloti) are not as effective in fixing atmospheric nitrogen as they are under moderate temperatures. It is also believed that the addition of nitrogen fertilizer inhibits nodulation of alfalfa seedlings. A replicated experiment was conducted under controlled environmental conditions at the University of Nevada-Reno, Reno, Nevada, USA, to determine the effects of nitrogen application on seedling growth and nodulation of alfalfa grown in a hot environment. Sterile sand was used as the growing media to which a complete nutrient solution minus nitrogen was applied volumetrically to each pot daily. In addition, half of the pots received NH4-$NO_3$, at the rate of 11.2 kg per ha at seeding and at two and four weeks after planting giving a total nitrogen application rate of 33.6 kg per ha during the seven-week experimental period. Rhizobia inoculant (R-12) consisted of a mixture of strains 171-15a, 1682c and 80 PI 265 of (Rhizobium meliloti). Inoculant was applied to the seeds prior to planting and to the sand media at two and four weeks after seeding. Twenty seeds were planted in pots 14.0 cm in diameter and 11.5 cm deep. Plants were thinned to ten plants per pot after emergence and were grown in a controlled environment chamber with a 16-hour light period. Soil temperature at 6 cm depth ranged from 17.4^{\circ}C.$ to 31.1^{\circ}C.$ and had a daily mean of 26.5^{\circ}C.$. Plants were harvested at weekly intervals for seven weeks. Root, shoot and total length, dry weight, volume and number of nodules per plant were determined. Root, shoot, and total length were greater in seedlings grown in soil where nigrogen was applied than that grown in soil to which no nitrogen was applied. The average size of the seedlings as determined by volume and weight was more than two times greater where plants were fertilized with nitrogen. Nodule number per seedling was also greater when nitrogen was applied compared to those which received no nitrogen. The differences were greater as the plants became older. The rhizobia did not fix enough nitrogen for adequate growth of seedlings. This is probably due to high temperature growing conditions that caused the rhizobia to become relatively ineffective as compared to cooler growing conditions. Data suggests it would be desirable to apply nitrogen at seeding when alfalfa is established under hot conditions that occur in mid- or late summer.

• Applied Chemistry for Engineering
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• v.10 no.3
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• pp.415-418
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• 1999

The characteristics of VRLA (valve regulated Iead-acid) battery with the tubular positive plate and gel type electrolyte were examined as a function of active material filling density. The filling density of positive plate was 3.2 g/mL, 3.4 g/mL, and 3.6 g/mL, respectively. These VRLA batteries were cycled with 100% DOD (depth of discharge) at the $C_5/5$ rate, followed by IU-type recharge with $I_{max}=0.2C_{10}/10$ and a final voltage V=2.40 V/cell. The test was performed in a thermostatic room at $25{\pm}1^{\circ}C$. The result indicated that the initial capacity was independent of active material filling density, i.e., the highest initial capacity was 3.4 g/mL of filling density and the lowest was 3.6 g/mL. On aspect of the cycle-life performance of the VRLA battery, the filling density of 3.6 g/mL was similar to that of 3.4 g/mL in the positive plate, and both were higher than that of 3.2 g/mL. Water-loss and degradation of the VRLA battery were decreased according to an increase of the filling density in the positive plate. The optimum filling density of the active material was 3.4~3.6 g/mL.

• Proceedings of the Korean Society of Toxicology Conference
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• 2006.11a
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• pp.46-54
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• 2006

The methylmercury (MeHg) is a toxic environmental pollutant, causing serious neurological and developmental effects in humans. Recent epidemiological studies have indicated that ingestion of MeHg in fish during pregnancy can result in neuroethological effects in the offspring. However, the mechanism underlying the MeHg-toxicity is not fully understood. To elucidate the mechanisms of toxicity of MeHg and of defense against MeHg, we searched for factors that determine the sensitivity of yeast cells to MeHg, and found that overexpression of Cdc34, a ubiquitin-conjugating enzyme (E2) that is a component of the ubiquitin-proteasome (UP) system, induces a resistance to MeHg toxicity in both yeast and human cells. The UP system is involved in the intracellular degradation of proteins. When Cdc34 is overexpressed in cells, ubiquitination reactions are activated and the degradation of certain proteins by the UP system is enhanced. Therefore, it seems likely that certain as-yet-unidentified proteins that increase MeHg toxicity might exist in cons and that toxicity might be reduced by the enhanced degradation of such proteins, mediated by the UP system, when Cdc34 is overexpressed. SCF ubiquitin-ligase is a component of UP system and consists of Skpl, the scaffold protein Cdc53, the RING-finger protein Hrt1, and one member of the family of F-box proteins. The F-box proteins directly bind to the substrates and are the determinants of substrate specificity of SCF. Therefore, we searched for the f-box protein that cofers resistance to MeHg, and found that overexpression of Hrt3 or Yi1224w induced resistance to MeHg toxicity in yeast cells. Since the protein(5) that enhance toxicity of MeHg might plausibly be induced in substrates of both f-box proteins, we next searched for substrate proteins that are recognized by Hrt3 or Y1r224w using two-hybrid screen. We found that Did3 or Crsl interacts with Hrt3; and Eno2 interacts with Yir224w. The yeast cells that overexpressed each those proteins showed hypersensitivity to MeHg, respectively, indicating that those proteins enhance the MeHg toxicity. Both Dld3 and Eno2 are proteins involved in the synthesis of pyruvate, and overexpression of both proteins might induce increase in interacellular levels of pyruvate. Deletion of Yi1006w that transports pyruvate into the mitochondria induced aresistance to MeHg. These results suggest that the promotion of the pyruvate irdlowinto the mitochondria might enhance MeHg toxicity. This study providesimportant keyfor the elucidauon of the molecular mechanism of MeHg toxicity.

• Journal of Korean Society on Water Environment
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• v.29 no.1
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• pp.88-96
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• 2013

Much attention has been needed in water resource management at the watershed due to drought and flooding issues caused by climate change in recent years. Increase in air temperature and changes in precipitation patterns due to climate change are affecting hydrologic cycles, such as evaporation and soil moisture. Thus, these phenomena result in increased runoff at the watershed. The Soil and Water Assessment Tool (SWAT) model has been used to evaluate rainfall-runoff at the watershed reflecting effects on hydrology of various weather data such as rainfall, temperature, humidity, solar radiation, wind speed. For bias-correction of RCP data, at least 30 year data are needed. However, for most gaging stations, only precipitation data have been recorded and very little stations have recorded other weather data. In addition, the RCP scenario does not provide all weather data for the SWAT model. In this study, two scenarios were made to evaluate whether it would be possible to estimate streamflow using measured precipitation and long-term average values of other weather data required for running the SWAT. With measured long-term weather data (scenario 1) and with long-term average values of weather data except precipitation (scenario 2), the estimate streamflow values were almost the same with NSE value of 0.99. Increase/decrease by ${\pm}2%$, ${\pm}4%$ in temperature and humidity data did not affect streamflow. Thus, the RCP precipitation data for Hongcheon watershed were bias-corrected with measured long-term precipitation data to evaluate effects of climate change on streamflow. The results revealed that estimated streamflow for 2055s was the greatest among data for 2025s, 2055s, and 2085s. However, estimated streamflow for 2085s decreased by 9%. In addition, streamflow for Spring would be expected to increase compared with current data and streamflow for Summer will be decreased with RCP data. The results obtained in this study indicate that the streamflow could be estimated with long-term precipitation data only and effects of climate change could be evaluated using precipitation data as shown in this study.

• Analytical Science and Technology
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• v.16 no.5
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• pp.349-357
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• 2003

The universality of low molecular weight metabolites (i.e. amino acids, steroid hormones) allows rapid and straightforward investigation of biochemistry of genetically un-characterized species. Thus in vivo metabolic profiling of amino acid in combination with multivariate data analysis (metabolomics) offers great potential in comparative biology. In this paper, amino acid profiles in biological fluid (media) were studied by using HPLC/FLD. HPLC procedure for amino acids require the formation of derivatives due to the low absorption of the free compounds. o-Phthalaldehyde (OPA) used in association with a thiol, such as 3-mercaptopropionic acid (3-MPA), is one of the most popular and sensitive reagents, which yield quickly fluorescent iso-indoles at room temperature. To improve unstability of OPA/3-MPA derivatization, we optimized injector programs for fixed injection times. Linear regressions for the standard curves were linear in the range 0.5 - 100.0 ppb, giving correlation coefficents above 0.99. The detection limit were 1.70 pmol(GLU) - 23.81 pmol(SER). It is practically useful when the amount of sample is very low on single cells.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.14 no.6
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• pp.57-69
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• 2011

The present paper deals with a research of life form and vegetation of the plants extant in the habitat of endangered Parnassius bremeri in Uiseong, Gyeongsangbuk-do with a view to restore their habitat and create substitute habitats. The research found that there are a total of 130 vascular plants including 47 families, 96 genera, 113 species, 1 subspecies and 16 varieties. In terms of their life form, hemicryptophytes (H) is the majority of dormancy form with 41 species or 31.54%, while $R_5$ is majority of radicoid form with 72 species or 55.38%, gravitational disseminule form ($D_4$) is majority among the disseminule forms with 68 species or 52.31% and erect type (e) is majority of growth form with 57 species or 43.85%. Major constituents of the habitat are Sedum kamtschaticum, Selaginella tamariscina, Orostachys japonica, Arundinella hirta, Cymbopogon tortilis var. goeringii, Themeda triandra var. japonica, Carex humilis, Indigofera kirilowii, Imperata cylindrica var. koenigii, Festuca ovina, Artemisia capillaris, etc., all of species which are in the initial stage of succession and show simultaneously features characteristic of vegetation on the rock. In the habitat 1. Sedum kamtschaticum- Selaginella tamariscina community and Pinus densiflora community are distributed whereas, in the habitat 2. Sedum kamtschaticum-Selaginella tamariscina community, Sedum kamtschaticum-Carex humilis community, Sedum kamtschaticum-Cymbopogon tortilis var. goeringii community and Sedum kamtschaticum-Imperata cylindrica var. koenigii community are growing and in the habitat 3. Sedum kamtschaticum-Selaginella tamariscina community, Selaginella tamariscina community, Sedum kamtschaticum-Indigofera kirilowii community and Quercus acutissima community are seen distributed there, too. Typical of food plants growing in the habitat is Sedum kamtschaticum, found mostly in the rocky area and its surroundings. Absorption plants are those that usually bloom in the season when imagoes emerge. From the research, it becomes evident that the blooming season of Sedum kamtschaticum, obviously a food plant, corresponds exactly to the emergence timing of imagoes. Cirsium japonicum var. ussuriense, Rosa multiflora, Erigeron annuus, Spiraea prunifolia var. simpliciflora are found to belong to these plants.

• Korean Journal of Ecology and Environment
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• v.50 no.1
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• pp.126-136
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• 2017

We describe characteristics of diversity and distribution of oak forests on the Daegok-cheon gorge of the Southeastern Korean Peninsula, the oldest prehistoric site, in view of a sort of food resources of acorns. The $Z{\ddot{u}}rich$-Montpellier School's method was adopted for field investigation on the oak dominant stands. A total of 20 phytosociological $relev\acute{e}s$ composed of 193 taxa were analyzed by syntaxonomy and ecological flora. The Daegok-cheon's oak forests occupied 36.9% ($513,374m^2$) of the surveyed area, and its 99% ($507,677m^2$) was Quercus variabilis and Q. serrata stands. Oak forests of the Daegok-cheon gorge are a kind of regional vegetation type characterizing by the high relative net contribution degree (r-NCD) of Platycarya strobilacea and Sapium japonicum, which are an edaphic type of the xerophilous and thermophilic oak forests. The region of the Daegok-cheon petroglyphs is defined as an ecoregion with rich acorn supply and abundant water resources, and the warmer environment, which attracts prehistoric man.