• Bulletin of the Korean Chemical Society
• /
• 제17권3호
• /
• pp.239-244
• /
• 1996

Mastoparan B (MP-B) that is a novel MP isolated from the hornet Vespa basalis, was studied as compared with MP, in terms of interaction with phospholipid bilayer and antimicrobial activity. MP-B has more hydrophilic amino acid residues in hydrophilic face of amphiphilic α-helical structure than MP. The both peptides exhibited considerably different effect on interaction with lipid bilayers, e.g. their conformation in the presence of acidic and neutral liposomes, dye-release ability from encapsulated liposomes, but on the whole the interaction mode was similar. On antimicrobial activity, MP had a strong activity against Gram-positive bacteria but no against Gram negative ones. Contrary to this, MP-B had a strong activity against Gram-positive and potent against Gram-negative ones. Since both peptides have almost same residues on the hydrophobic side, such more hydrophilic surface on the molecule seems to lead to the subtle change in its interaction with membranes, resulting in the alternation in its biological activity.

• 한국축산식품학회지
• /
• 제39권4호
• /
• pp.576-584
• /
• 2019

This study was performed to evaluate the physicochemical properties of myofibrillar protein (MP) gels containing pork skin gelatin at different salt concentrations. MP gels were prepared to the different salt levels (0.15, 0.30, and 0.45 M) with or without 1.0% of pork skin gelatin. Cooking yield (CY), gel strength, shear stress were measured to determine the physical properties, and SDS-polyacrylamide gel electrophoresis, scanning electron microscopy, fourier transform infrared spectroscopy, sulfhydryl group and protein surface hydrophobicity was performed to figure out the structural changes among the proteins. The addition of gelatin into MP increased CYs and shear stress. MP at 0.45 M salt level had the highest CY and shear stress, as compared to MPs at lower salt concentrations. As the salt concentration of MP gels increased, the microstructure became the compact and wet structures, and decreased the amount of ${\alpha}-helix$/unordered structures and ${\beta}-sheet$. MP with gelatin showed a decreased amount of ${\alpha}-helix$/unordered structures and ${\beta}-sheet$ compared to MP without gelatin. The addition of gelatin to MP did not affect the sulfhydryl group, but the sulfhydryl group decreased as increased salt levels. MP mixtures containing gelatin showed a higher hydrophobicity value than those without gelatin, regardless of salt concentration. Based on these results, the addition of gelatin increased viscosity of raw meat batter and CY of MP gels for the application to low salt meat products.

• 한국수산과학회지
• /
• 제36권2호
• /
• pp.100-106
• /
• 2003

This study was conducted to investigate the supplementary effects of vitamin C and E to moist pellet on growth and body composition in juvenile olive flounder (Paralichthys olivaceus). Four groups of diet made : moist pellet (MP) diet group was used as the control group where the ratio of raw feed and commercial compound meal is 5:5 $(CP\;30\%,\;CL\;17\%),$ vitamin C added group (VC), vitamin I added group (VE) and vitamin C and I added group (VCE). The supplementary amount of vitamin C and E corresponded to 1000 mg/kg (dry wt.) and 220mg/kg (dry wt.), respectively. Vitamin C was destroyed $50\%$ and vitamin E was destroyed $20\%$ for manufacturing process. After 8 weeks feeding trial, weight gain was $121.9\%$ in MP group, while it ranged from 180.5 to $184.9\%$ in the VC, VE and VCE group. Feed efficiency was $71.\6%$ in MP group, whereas it ranged from 78.7 to $80.6\%$ in the VC, VE and VCE group, weight gain and feed efficiency of fish fed vitamin supplemented diets were significantly higher than control group and no significant difference among the vitamin added groups was observed (p>0.05). There were no significant differences in moisture, crude protein, crude lipid, crude ash, condition factor (CF), hepatosomatic index (HSI), and visceraweight index (VWI) of body composition. Hemoglobin (Hb) was $3.79\%$ in MP group, whereas $4.99\%$ in VC group, $4.55\%$ in VE group and $5.07\%$ in VCE group; Hb was significantly higher in the vitamin added groups than the control group (p<0.05). Vitamin C retention in liver was 18.1, 20.7, 46.6, 54.0 mg/kg in MP, VE, VC and VCE groups, respectively. Vitamin E retention in liver was 25.9, 53.8, 88.2, 124.5 mg/kg in MP, VC, VE and VCE groups, respectively. These results indicate that supplementation of vitamin C and E to moist pellet diet significantly improved growth and feed efficiency of olive flounder, and the mixed addition of vitamin C (1,000 mg/kg dry wt.) and vitamin E (220 mg/kg dry wt.) did not lead to the synergy effect.

• 정보처리학회논문지D
• /
• 제9D권2호
• /
• pp.323-328
• /
• 2002

최근 초고속 통신망을 이용한 인터넷의 대중화와 더불어 인터넷을 기반으로 하는 전자상거래가 활발해지고 있다. 특히 인터넷을 통한 MP3 데이터 등의 멀티미디어 컨텐츠의 유통은 많은 연구의 대상이 되고 있다. 하지만 기존의 AOD(Audio On Demand) 시스템들은 실질적인 불법 복제방지와 저작권 보호에 미흡한 단점이 있다. 따라서 본 논문에서는 공개키 기반 구조(PKI: Public Key Infrastructure)에 기초하여 실질적인 불법복제방지와 저작권을 보호하는 AOD 시스템을 제안한다. 제안된 기법은 사용자의 공개키를 이용하여 MP3 데이터를 전송함으로써 전송 도중 제 3자로부터의 공격에 대응할 수 있고, 정당한 사용자 외에는 MP3 데이터를 사용하지 못하도록 함으로써 사용자와 판매자의 권리를 보장한다.

• 한국전자거래학회:학술대회논문집
• /
• 한국전자거래학회 1999년도 종합학술대회발표논문집
• /
• pp.589-600
• /
• 1999

인터넷 전자상거래 시장의 성장에 따라 디지털 상품에 대한 온라인 판매가 가능하게 되었다. 그러나 현재의 디지털 상품 온라인 유통 체계는 저작권 및 사용자 사용권을 제대로 보장해 주지 못하고 있다. 디지털 상품 중에서도 가장 큰 이슈인 MP3 미디어 데이터는 불법 재생 및 복제에 대해 무방비 상태에 놓여 있다고 해도 과언이 아니다. 본 논문에서는 동적 사용권 관리 기술을 활용한 효율적인 MP3 미디어 데이터 온라인 유통 시스템을 제안한다. 제안한 시스템은 사용권 보호를 원하는 사용자와 저작권 및 불법 재생과 배포의 차단을 원하는 저작사 및 지적 재산권 소유자의 요구 조건을 충족시켜 준다. 추가적으로 MP3 미디어 데이터의 불법 재생 및 배포를 차단할 수 있는 MP3 미디어 데이터 유통 시스템의 궁극적인 모델을 제시한다.

• 한국산업정보학회논문지
• /
• 제8권4호
• /
• pp.26-30
• /
• 2003

대칭이고 큰 희소 행렬(Large Sparse Matrices)에 대한 가장 작거나 또는 가장 큰 고유치(Eigenvalues)들을 구하기 위해서 Lanczos 방법이 많이 이용된다. MP(Message Passing) 병렬 컴퓨터에서 global communications은 계산 속도를 떨어뜨린다. 본 논문에서는 s-step Lanczos 알고리즘을 소개하였으며 이 s-step 방법은 기존의 Lanczos 알고리즘에 의해 생성된 행렬에 유사한 축소 행렬을 생성하며 s-step Lanczos 알고리즘에서 한번의 반복은 기존의 Lanczos 알고리즘의 s 번 반복에 해당한다. s-step 방법은 global communications을 최소화하였으며 기존의 알고리즘에 비해 뛰어난 병렬 성질을 가진다. 알고리즘들은 Cray T3E에서 수행되었으며 그 결과를 볼 수 있다.

• 핵의학기술
• /
• 제19권1호
• /
• pp.72-80
• /
• 2015

Estradiol($E_2$)은 월경 주기와 배란유도 및 불임 및 폐경을 진단하는데 유용한 지표이다. 따라서 정도인증 평가 지표인 정밀도(precision), 회수율(accuracy), 예민도(sensitivity) 및 타 키트와의 상관성(comparison) 비교를 통하여 우수한 키트를 선정하여 사용함으로서 핵의학검사실 표준화와 질 향상을 도모하고자 본 연구를 시행하였다. 핵의학검사실에서 사용하고 있는 방사면역측정법(Radioimmunoassay; RIA)으로 SIEMENS (Coat-A-Count Estradiol, USA), DIAsource (Immunoassays S.A.. Belgium), AMP (As bach Medical Products GMBH, Germany), BECKMAN COULTER (IMMUNOTECH s.r.o. Czech Republic), Beckman Coulter Ultra Sensitive Estradiol (IMMUNOTECH s.r.o. Czech Republic), CIS Bio (France), MP (ICN Biomedicals, Germany) 7개 키트와 화학발광면역측정법으로 검사하는 E170 (Roche Diagnostics, Germany), Architect (ABBOTT, USA) 2종류의 자동화장비로 측정하였다. 측정 내 정밀도는 키트별로 농도에 따라 다르게 측정 되었으며, 측정값이 낮은 저농도에서는 10.9~13.6% 변이계수를 보였으며, 중, 고농도에서는 모든 키트가 10% 이내의 결과를 보였다. 키트간 상관성 평가를 위한 ANOVA 분석에서 저농도, 중농도, 고농도 검체 각각에 대하여 대부분 키트 간에 유의한 상관관계를 보였으나 일부 키트에서는 유의하지 않은 결과를 보이기도 하였다. 측정 간 정밀도는 측정값이 낮은 저(Low) 농도에서는 10.8~12.3% 변이계수를 보였으며, 중(Medium), 고(High)농도에서는 모든 키트가 10% 이내의 결과를 보였다. 회수율은 IEMENS $92.7{\pm}12.4%$, DIAsou rce $101.4{\pm}18.4%$, AMP $95.1{\pm}11.5%$, BECKMAN COULTER $108.4{\pm}18.5%$, BECKMAN COULTER Ultra Sensitive $104.2.{\pm}13.5%$, CIS Bio $101.3{\pm}11.6%$, MP $93.1{\pm}13.2%$ 이다. 예민도(pg/mL)는 SIEMENS 7.5, DIAsource 6.2, AMP 5.7, BECKMAN COULTER 6.2, BECKMAN COULTER Ultra Sensitive 5.3, CIS Bio 4.5, MP 5.5 이다. 상관성에서는 저농도에서는 MP 키트가 타 키트에 비해 다소 높게 측정되었으며, 중, 고농도에서는 E170 장비가 다소 높게 측정되었다. 외부정도관리 숙련도 시료를 이용한 측정값에서는 타 키트에 비하여 SIEMENS 키트는 낮은 경향을 보였으며, E170, Architect, MP 키트들은 다소 높은 경향을 보였다. $E_2$ 검사 측정 키트 및 방법들은 전체적으로 정밀도, 회수율, 민감도 등의 지표에서 검사에 이용하는 데 무리가 없으나, 키트 변경 시에는 검사의 일관성 유지를 위하여 상호 상관성을 고려하여야 할 것이다.

• 한국축산식품학회지
• /
• 제42권5호
• /
• pp.849-860
• /
• 2022

The myofibril protein (MP) isolate-saccharide graft reactions was prepared using the Maillard reaction with saccharides. The effects of various saccharides on protein functionality and quality of the Maillard reaction were investigated and compared with those of MP. The grafting degree of the MP isolate-saccharide graft reaction was significantly higher in the reducing sugar-treated groups (lactose, glucose, fructose, and palatinose). The browning intensity of the MP isolate-saccharide graft reaction with fructose, sucrose, and erythitol was higher than that observed in the control reaction (p<0.05). MP that reacted with reducing sugars (glucose, fructose, palatinose, and lactose) had fainter bands than MP that reacted with non-reducing sugars (sucrose, erythitol, trehalose, sorbitol, and xylitol). MPs conjugated with glucose exhibited higher protein solubility. The palatinose and lactose treatments were maximum in water binding capacity, though no significant difference in oil binding capacity among the saccharide treatments was observed. The emulsion stability of the MP isolate-saccharide graft reaction with palatinose and erythitol was higher than that of the control reaction. Therefore, reducing sugars have good protein functionality in the MP isolate-saccharides graft reaction.

• 한국축산식품학회지
• /
• 제43권6호
• /
• pp.1031-1043
• /
• 2023

The purpose of this study was to investigate the functional properties of salt-soluble proteins obtained from Protaetia brevitarsis (PB) and Tenebrio molitor (TM) larvae, the interaction between these proteins and pork myofibrillar protein (MP) in a gel system. The gel properties of salt-soluble protein extracts showed that the PB had a higher viscosity than the TM protein. However, the TM protein had higher gel strength compared with the PB protein. The gelation characteristics of the pork MP gel systems added with lyophilized insect salt-soluble protein powder showed to decrease slightly viscosity compared with MP alone. Adding the TM or PB protein powder did not affect the pork MP's hydrophobicity and sulfhydryl group levels. Furthermore, the protein bands of the MP did not change with the type or amount of insect salt-soluble protein. The cooking yields of the pork MP gels containing PB or TM protein powder were higher than those without insect protein. Regardless of the type of insect salt-soluble protein added, the pork MP's gel strength decreased. Furthermore, as the level of insect powder increased, the surface protein structure became rough and porous. The results demonstrated that proteins extracted from PB and TM larvae interfered with the gelation of pork MP in a gel system.

• 한국중재학회지:중재연구
• /
• 제16권3호
• /
• pp.191-216
• /
• 2006

It has been talked about the responsibility of the South-North Korean e-market place(e-MP) collaboration project every you since 2003. However, the feasibility as the business has not been dealt with in detail due to the uncertain environment as well as the complicated procedures of the project. The research focuses on the workable mechanism of the South-North Korean E-Market Place collaboration project. The feasibility of the project results from the newly installed fiber telecommunication network as well as the 1 billion dollars of the trading volume between South and North Korea. The research analyzes on the capabilities as well as willingness of the South and North Korea for the e-MP, and concludes with two paradigms of the future tasks. First, the unified but step-wise structure should be made to promote the project by the political committee (South and North Korea EC Committee) and the economical administration body (South and North Korea EC Corporation). Second, the market-oriented resolution system such as South and North Korea Arbitration Body should be involved to create legal environment. Above all, the discussion should be on the main body of e-MP settlement to abolish the psychological as well as practical barriers to enter the South-North Korean electronic commerce.