• The Plant Pathology Journal
• /
• v.37 no.1
• /
• pp.47-56
• /
• 2021

Plants protect against viruses through passive and active resistance mechanisms, and in most cases characterized thus far, natural recessive resistance to potyviruses has been mapped to mutations in the eukaryotic initiation factor eIF4E or eIF(iso)4E genes. Five eIF4E copies and three eIF(iso)4E copies were detected in Brassica rapa. The eIF4E and eIF(iso)4E genes could interact with turnip mosaic virus (TuMV) viral protein linked to the genome (VPg) to initiate virus translation. From the yeast two-hybrid system (Y2H) and bimolecular fluorescence complementation (BiFC) assays, the TuMV-CHN2/CHN3 VPgs could not interact with BraA.eIF4E.a/c or BraA.eIF(iso)4E.c, but they could interact with BraA.eIF(iso)4E.a in B. rapa. Further analysis indicated that the amino acid substitution L186F (nt T556C) in TuMV-UK1 VPg was important for the interaction networks between the TuMV VPg and eIF(iso)4E proteins. An interaction model of the BraA. eIF(iso)4E protein with TuMV VPg was constructed to infer the effect of the significant amino acids on the interaction of TuMV VPgs-eIF(iso)4Es, particularly whether the L186F in TuMV-UK1 VPg could change the structure of the TuMV-UK1 VPg protein, which may terminate the interaction of the BraA.eIF(iso)4E and TuMV VPg protein. This study provides new insights into the interactions between plant viruses and translation initiation factors to reveal the working of key amino acids.

• The Plant Pathology Journal
• /
• v.23 no.4
• /
• pp.281-286
• /
• 2007

Interactions between viral proteins and host proteins are essential for virus replication. Especially, translation of viral genes completely depends on the host machinery. In potyviruses, interactions of genome-linked viral protein (VPg) with host translation factors including eIF4E, eIF(iso)4E, and poly(A)-binding protein (PABP) has previously been characterized. In this study, we investigated interactions between Soybean mosaic virus (SMV) viral proteins and host translation factors by yeast two-hybrid system. SMV VPg interacted with eIF4E, eIF(iso)4E, and PABP in yeast two-hybrid system, while SMV helper component proteinase (HC-pro) interacted with neither of those proteins. The interaction between SMV NIb and PABP was also detected. These results are consistent with those reported previously in other potyviruses. Interestingly, we found reproducible and specific interactions between SMV coat protein (CP) and PABP. Deletion analysis showed that the region of CP comprising amino acids 116 to 206 and the region of PABP comprising amino acids 520 to 580 are involved in CP/PABP interactions. Soybean library screening with SMV NIb by yeast two-hybrid assay also identified several soybean proteins including chlorophyll a/b binding preprotein, photo-system I-N subunit, ribulose 1,5-biphosphate carboxylase, ST-LSI protein, translation initiation factor 1, TIR-NBS type R protein, RNA binding protein, ubiquitin, and LRR protein kinase. Altogether, these results suggest that potyviral replicase may comprise a multi-protein complex with PABP, CP, and other host factors.

• Proceedings of the Korean Operations and Management Science Society Conference
• /
• 2003.05a
• /
• pp.208-216
• /
• 2003

ISO 14649 (data model for STEP-NC) is a new interface scheme or language for CAD-CAM-CNC chain under established by ISO TC184 SCI. Up to this point, the new language is mainly made for milling and turning, and other processes such as EDM will be completed in the future. Upon completion, it will be used as the international standard language for e-manufacturing paradigm by replacing the old machine-level language, so called M&G code used since 1950's. With the rich information contents included in the new language, various intelligent functions can be made by the CNC as the CNC knows what-to-make and how-to-make. In particular, On-Machine Inspection required for quality assurance in the machine level, can be done based on the information of feature­based tolerance graph. Previously, On-Machine inspection has been investigated mainly for milling operation, and only a few researches were made for turning operation without addressing the data model. In this thesis, we present a feature-based on-machine inspection process by the 4 Tasks: 1) proposing a new schema for STEP-NC data model, 2) converting the conventional tolerance scheme into that of STEP-NC, 3) modifying the tolerance graph such that the tolerance can be effectively measured by the touch probe on the machine, and 4) generating collision-free tool path for actual measurement. Task 1 is required for the incorporation of the presented method in the ISO 14649, whose current version does not much include the detailed schema for tolerance. Based on the presented schema, the tolerance represented in the conventional drafting can be changed to that of STEP-NC (Task 2). A special emphasis was given to Task 3 to make the represented tolerance accurately measurable by the touch probe on the machine even if the part setup is changed. Finally, Task 4 is converting the result of Task into the motion of touch probe. The developed schema and algorithms were illustrated by several examples including that of ISO 14649 Part 12.

• International Journal of Internet, Broadcasting and Communication
• /
• v.14 no.4
• /
• pp.132-139
• /
• 2022

The development of the fourth industrial revolution and logistics 4.0 related technology, the growth of the e-commerce market, and the transition to a non-face to face society due to the pandemic are accelerating the growth of the logistics industry. Due to the growth of the logistics industry, various services are emerging to meet the requirements of the market, and research and technology development related to the parcel storage, which is an important element of the last mile service, is also underway. In the past, if it was difficult to deliver the goods directly to the recipient, the parcel storage installed near the delivery location was used, but the usability was not good and the storage of the goods was limited. In addition, the existing parcel storage has a lot of functional limitations compared to the advanced logistics technology, so it is necessary to develop a device that improves it. Therefore, this study conducted to secure safety for unmanned parcel storage devices with robot technology to improve usability and functionality in line with the advanced logistics industry. Based on ISO 10218, an industrial robot related standard, risk identification studies were conducted to derive results that contribute to the development of devices under development.

• The Journal of Society for e-Business Studies
• /
• v.4 no.1
• /
• pp.87-101
• /
• 1999

To operate the automatic devices of manufacturing process more effectively and to solve the needs of the resource sharing, network technology is applied to the control devices located in common manufacturing zone and operated by connecting them. In this paper, functional standard of the network layers are set as physical and data link layer of IEEE 802.2, 802.4, and VMD application layer and ISO-CIM reference model. Then, they are divided as minimized architecture, designed as group objects which perform group management and service objects which organizes and operates the group. For the stability in this network, this paper measures the variation of data packet length and node number and analyzes the variated value of the waiting time for the network operation. For the method of the analysis, non-exhausted service method are selected, and the arrival rates of the each data packet to the nodes that are assumed to form a Poission distribution. Then, queue model is set as M/G/1, and the analysis equation for waiting time is found. For the evalution of the performance, the length of the data packet varies from 10 bytes to 100 bytes in the operation of the group management network, the variation of the wating time is less than 10 msec. Since the waiting time in this case is less than 10 msec, response time is fast enough. Furthermore, to evaluate the real time processing of the group management network, it shows if the number of nodes is less than 40, and the average arrival time is less than 40 packet/sec, it can perform stable operation even taking the overhead such as software delay time, indicated packet service, and transmissin safety margin.

• The Plant Pathology Journal
• /
• v.39 no.1
• /
• pp.28-38
• /
• 2023

Plant viruses are responsible for worldwide production losses of numerous economically important crops. The most common plant RNA viruses are positivesense single-stranded RNA viruses [(+)ss RNA viruses]. These viruses have small genomes that encode a limited number of proteins. The viruses depend on their host's machinery for the replication of their RNA genome, assembly, movement, and attraction to the vectors for dispersal. Recently researchers have reported that chloroplast proteins are crucial for replicating (+)ss plant RNA viruses. Some chloroplast proteins, including translation initiation factor [eIF(iso)4E] and 75 DEAD-box RNA helicase RH8, help viruses fulfill their infection cycle in plants. In contrast, other chloroplast proteins such as PAP2.1, PSaC, and ATPsyn-α play active roles in plant defense against viruses. This is also consistent with the idea that reactive oxygen species, salicylic acid, jasmonic acid, and abscisic acid are produced in chloroplast. However, knowledge of molecular mechanisms and functions underlying these chloroplast host factors during the virus infection is still scarce and remains largely unknown. Our review briefly summarizes the latest knowledge regarding the possible role of chloroplast in plant virus replication, emphasizing chloroplast-related proteins. We have highlighted current advances regarding chloroplast-related proteins' role in replicating plant (+)ss RNA viruses.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.19 no.5
• /
• pp.570-576
• /
• 2018

In reinforced concrete structures, the joints of ordinary rebars are usually lap joints, which are bound by binding wires with rebars, and mechanical joints by couplers. In domestic design standards (concrete design code), welded lap joints are restricted for ordinary rebars, but overseas standards allow welded lap joints of ordinary rebars through pre-heating. This study investigated the domestic and international standards/criteria and evaluated the fracture strength by performing the tensile test on the lap welded joint of SD400 grade rebars, which is used the most in the construction sites. The weld length of the specimen for weld lap joints is based on the minimum weld length (8d) given in the KS standard (KS B ISO 17660-1). According to AWS D1.4, the preheating temperature was set to $150^{\circ}C$ for D19 and below, and $260^{\circ}C$ for D22 and above. In the test results, the tensile strength of rebars with welded lap joints exceeded the required strength (125% of the yield strength) according to the concrete design code. To analyze the effect of preheating, the tensile strength of the welded rebars after preheating was not significantly different from that of the welded rebars without preheating. The carbon equivalent content (Ceq) of the rebars used in the test was 0.45% or less. Under AWS D1.4, no preheating is required if the carbon equivalent is less than 0.45%. All specimens with a welded lap length of 8d failed by a bar fracture. The effect of preheating was confirmed to be insignificant due to the low carbon equivalent of the rebar.

• Analytical Science and Technology
• /
• v.18 no.4
• /
• pp.271-277
• /
• 2005

Urea in blood has been measured as an effective marker for diagnosis of renal function. Urea which is e end-product of nitrogen containing metabolites such as proteins is filtered through glomeruli of kidneys and then excreted as urine. If the renal function is deteriorated, the urea concentration in blood will be increased, from which the healthiness of renal function is judged. In order to improve the confidence of diagnosis results, the results must keep traceability chain to certified reference materials, which was certified by primary reference method. In this study, we proposed isotope dilution-liquid chromatography/mass spectrometry (ID-LC/MS) as a candidate primary method, in which $15^N_2$-urea is used as an internal reference material. The developed method is highly accurate in principle and is convenient as it does not require cumbersome derivatization. 0.1 mmol/L ammonium chloride was selected as a mobile phase for HPLC because it provided low interference in MS analysis of relatively low molecular weighted urea. HPLC and MS were connected with an electrospray ionization (ESI) interface of positive mode, which provided high sensitivity and reproducibility. The developed method was validated with internationally recognized reference materials, and we have obtained satisfactory results in an international ring trial. The expanded uncertainty calculated according to ISO guide was 1.8% at 95% confidence interval. The developed method is being used as a primary reference measurement method such as for certification of serum certified reference materials (CRMs).

• Journal of Korean Society of Occupational and Environmental Hygiene
• /
• v.19 no.3
• /
• pp.213-232
• /
• 2009

This document was prepared to review and summarize the analytical methods for airborne and bulk asbestos. Basic principles, shortcomings and advantages for asbestos analytical instruments using phase contrast microscopy(PCM), polarized light microscopy(PLM), X-ray diffractometer (XRD), transmission electron microscopy(TEM), scanning electron microscopy(SEM) were reviewed. Both PCM and PLM are principal instrument for airborne and bulk asbestos analysis, respectively. If needed, analytical electron microscopy is employed to confirm asbestos identification. PCM is used originally for workplace airborne asbestos fiber and its application has been expanded to measure airborne fiber. Shortcoming of PCM is that it cannot differentiate true asbestos from non asbestos fiber form and its low resolution limit ($0.2{\sim}0.25{\mu}m$). The measurement of airborne asbestos fiber can be performed by EPA's Asbestos Hazard Emergency Response Act (AHERA) method, World Health Organization (WHO) method, International Standard Organization (ISO) 10312 method, Japan's Environmental Asbestos Monitoring method, and Standard method of Indoor Air Quality of Korea. The measurement of airborne asbestos fiber in workplace can be performed by National Institute for Occupational Safety and Health (NIOSH) 7400 method, NIOSH 7402 method, Occupational Safety and Health Administration (OSHA) ID-160 method, UK's Health and Safety Executive(HSE) Methods for the determination of hazardous substances (MDHS) 39/4 method and Korea Occupational Safety and Health Agency (KOSHA) CODE-A-1-2004 method of Korea. To analyze the bulk asbestos, stereo microscope (SM) and PLM is required by EPA -600/R-93/116 method. Most bulk asbestos can be identified by SM and PLM but one limitation of PLM is that it can not see very thin fiber (i.e., < $0.25{\mu}m$). Bulk asbestos analytical methods, including EPA-600/M4-82-020, EPA-600/R-93/116, OSHA ID-191, Laboratory approval program of New York were reviewed. Also, analytical methods for asbestos in soil, dust, water were briefly discussed. Analytical electron microscope, a transmission electron microscope equipped with selected area electron diffraction (SAED) and energy dispersive X-ray analyser(EDXA), has been known to be better to identify asbestiform than scanning electron microscope(SEM). Though there is no standard SEM procedures, SEM is known to be more suitable to analyze long, thin fiber and more cost-effective. Field emission scanning electron microscope (FE-SEM) imaging protocol was developed to identify asbestos fiber. Although many asbestos analytical methods are available, there is no method that can be applied to all type of samples. In order to detect asbestos with confidence, all advantages and disadvantages of each instrument and method for given sample should be considered.