• Quantitative Bio-Science
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• v.37 no.2
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• pp.133-141
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• 2018

Graphical lasso is one of the most popular methods to estimate a sparse precision matrix, which is an inverse of a covariance matrix. The objective function of graphical lasso imposes an ${\ell}_1$-penalty on the (vectorized) precision matrix, where a tuning parameter controls the strength of the penalization. The selection of the tuning parameter is practically and theoretically important since the performance of the estimation depends on an appropriate choice of tuning parameter. While information criteria (e.g. AIC, BIC, or extended BIC) have been widely used, they require an asymptotically unbiased estimator to select optimal tuning parameter. Thus, the biasedness of the ${\ell}_1$-regularized estimate in the graphical lasso may lead to a suboptimal tuning. In this paper, we propose a two-staged bias-correction procedure for the graphical lasso, where the first stage runs the usual graphical lasso and the second stage reruns the procedure with an additional constraint that zero estimates at the first stage remain zero. Our simulation and real data example show that the proposed bias correction improved on both edge recovery and estimation error compared to the single-staged graphical lasso.

• Animal Bioscience
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• v.36 no.3
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• pp.429-440
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• 2023

Objective: 12-oxo-5Z,8Z,10E,14Z-eicosatetraenoic acid (12-KETE), a metabolite of arachidonic acid, is a strong candidate signal for placenta separation following calf discharge at delivery. In the present study, the effects of 12-KETE on bovine trophoblast cells were investigated to determine its function in the placentome at delivery. Methods: Bovine trophoblast cells derived from blastocysts were used. They were cocultured with or without fibroblasts derived from bovine placentome and/or bovine uterine epithelial cells. 12-KETE was added to the culture medium. Results: Bovine trophoblast cells contained binucleate cells and strongly expressed caudal type homeobox 2 (CDX-2) genes. Addition of 12-KETE to the trophoblast cell colony without feeder cells or that on a fibroblast monolayer induced rapid exfoliation of the colony. After 12-KETE addition, trophoblast cells emitted strong fluorescence caused by the degradation of dye-quenched collagen, indicating that 12-KETE activated matrix metalloproteinase of the trophoblast cells. Exfoliated cell colonies were stained with YOPRO-1, but not propidium iodide (PI). Moreover, DNA fragmentation and Bcl-2 associated X protein (Bax) gene (apoptosis stimulator) upregulation were observed in exfoliated cells, indicating that 12- KETE induced trophoblast cell apoptosis. These results were consistent with previous in vivo observations; however, even a lower concentration of 12-KETE activated trophoblast protease. Meanwhile, fibroblasts derived from the bovine placentome converted arachidonic acid to 12-KETE. Conclusion: These observations indicate that 12-KETE may serve as a signal for placenta separation at delivery.

• Proceedings of the Korean Powder Metallurgy Institute Conference
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• 2006.09b
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• pp.1026-1029
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• 2006

Particulate reinforced titanium composites were produced by PM rout. Differents volumetric percentages of TiN reinforcements were used, 5,10,15 vol%. Samples were uniaxial pressed and vacuum sintered at differents temperatures between $1200-1300^{\circ}C$. Density, porosity, shrinkage, mechanical properties and microstructure were studied. Elastic properties and strength resistance were analysed by flexural strength and tension tests, and after the test, fractured samples were analysed too, obtaining a correlation between the fracture, interparticulated or intraparticulated, and the reinforcement addition.. Hardness and microhardness test were applied too, in order to complete the study about mechanical properties. In order to study wear resistance pin-on-disc test were used. In addition, the temperature influence, the reactivity between matrix and reinforcement, and the microstructures developed were observed by optical and electron microscopy.

• Advanced Composite Materials
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• v.16 no.4
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• pp.315-334
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• 2007

In the present study, chopped henequen natural fibers without and with surface modification by electron beam (E-beam) treatment were incorporated into a polypropylene matrix. Prior to composite fabrication, a bundle of raw henequen fibers were treated at various E-beam intensities from 10 kGy to 500 kGy. The effect of E-beam intensity on the interfacial, mechanical and thermal properties of randomly oriented henequen/polypropylene composites with the fiber contents of 40 vol% was investigated focusing on the interfacial shear strength, flexural and tensile properties, dynamic mechanical properties, thermal stability, and fracture behavior. Each characteristic of the material strongly depended on the E-beam intensity irradiated, showing an increasing or decreasing effect. The present study demonstrates that henequen fiber surfaces can be modified successfully with an appropriate dosage of electron beam and use of a low E-beam intensity of 10 kGy results in the improvement of the interfacial properties, flexural properties, tensile properties, dynamic mechanical properties and thermal stability of henequen/polypropylene composites.

• International Commerce and Information Review
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• v.9 no.3
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• pp.59-79
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• 2007

This paper is to identify whether the mechanism of the real market as an off-line market developed from the transaction cost theory from previous research is applied to on-line market, such as usiness to Business e-marketplaces. With three factors(parties, product, environments) selected from previous research, four cases picked up from all B2B e-marketplace sites as of April 2007, in the view of Business to Business Matrix which presented by Kaplan & Sawhney(2000), were studied. They were accompanied by interview with CEO and team manager, and they explained their business and revenue model of the company. From the interview, the relation between three factors and usiness to Business e-marketplace was observed by taking sub-factors into consideration; parties -asset specificity, information asymmetry, product factors - standardization, price stability, and environments factors - competition, market uncertainty. The implications of this study are to analyze the relation of the transaction theory in offline and online. Also, this is the first study that analyzed it. In the future, another research based on this research will be studied.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.9 no.1
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• pp.108-114
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• 1998

This paper presents the design and fabrication of waveguide E-plane metal insert bandpass filter for milli-meter waves which is analyzed transmission characteristics by calculating the generalized scattering matrix using mode-matching method. The bandpass filter was fabricated to verify the proposed method by the WR-34 rectangular waveguide to operate in 30.085 ~ 30.885 GHz. The fabricated waveguide E-plane metal insert band- pass filter was established the fact that it is so adaptive for milli-meter waves due to the measured data closely consistent with the results of analysis using mode-matching method, that is, the maximum value of insertion loss is 0.8 dB and the return loss is below -15 dB.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.27 no.6
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• pp.510-522
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• 2005

To assess the clinical applicability of bio-artificial mucosa which was made with autologous oral keratinocytes and human acellular dermal matrix, the formation of basement membrane and stratification of oral keratinocytes were evaluated. Six New Zealand white rabbits (around 2kg in weight) were anesthetized and its buccal mucosa was harvested (1.0 $\times$ 0.5cm size). Oral keratinicytes were extracted and cultured primarily with the feeder layer of pretreated NIH J2 3T3 fibroblast. These confluent cells were innoculated on the human acellular dermal matrix and cultured in multiple layer by air-rafting method. After 3, 5, 7, 10, 14 days of culture, each cultured bio-artificial mucosa was investigated the number of epthelial layer of by H&E stain and toluidine blue stain. The immuhohistochemical methods were used to evaluate the cell division capacity, the formation of basement membrane, and it's property of specific cells (PCNA, cytokeratin 14, laminin). Transmission electromicroscopy was used for the attachment between cells and matrix with the number of hemidesmosome. In result, the numbers of layer of stratified growth of oral keratinocyte cultured on the human acellular dermal matrix and the number of hemidesomal attachment between epithelial cells and human acellular dermal matrix were similar to the layers of normal oral mucosa after 10 days of culture. The cell division rate, basement membrane formation and proliferation rate increased as culture period increased. With these results, bio-artificial mucosa with autologous oral epithelial cells cultured on the acellular dermal matrix had clinically adaptable properties after 10 days' culture and this new bio-artificial mucosa model with relatively short culture time can be expected clinical applicability.

• Communications for Statistical Applications and Methods
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• v.6 no.1
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• pp.327-336
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• 1999

In a multinomial sampling scheme some categories may be observed as partially classified because of technical or economic reasons. In this paper the maximum likelihood estimators(M.L.E) of multinomial probabilities are obtained when some frequencies are merged. We obtained the M.L.E and a method to evaluate the information gained by including merged frequencies in M.L.E we obtained an estimator of the covariance matrix and it is used to examine the information gained by including the merged frequency counts in estimating the cell probabilities. When certain individual frequency counts are missing a method is proposed for estimating the cell probabilities using EM algorithms.

• The Journal of the Acoustical Society of Korea
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• v.15 no.1E
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• pp.59-64
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• 1996

When the input-output record is available, the identification of a bilinear system is considered. It is assumed that the input is noise free and the output is contaminated by an additive noise. It is further assumed that the covariance matrix of the noise is known up to a factor of proportionality. The extended generalized total least squares (e-GTLS) method is proposed as one of the consistent estimators of the bilinear system parameters. Considering that the input is noise-free and that bilinear system equation is linear with respect to the system parameters, we extend the GTLS problem. The extended GTLS problem is reduced to an unconstrained minimization problem, and is solved by the Newton-Raphson method. We compare the GTLS method and the e-GTLS method in the point of the accuracy of the estimated system parameters.

• 한국정보디스플레이학회:학술대회논문집
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• 2005.07a
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• pp.236-240
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• 2005

The commercialization of an active matrix e-reader display using E Ink microencapsulated electrophoretic (MEP) ink marked a big step towards comfortable reading over an extended period of time in an electronic book, as the high resolution display, jointly realized by Philips, E Ink and Toppan, has a true paper-like look and feel. Alternative electrophoretic material systems are being developed by SiPix and Bridgestone and progress has been made in the past two years. In this paper, electrophoretic material systems are briefly reviewed, after which the perspectives and challenges of electrophoretic displays are addressed and methods for generating gray tones are presented and discussed.