• Journal of Periodontal and Implant Science
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• v.30 no.2
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• pp.347-360
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• 2000

Bone remodeling results from the combined process of bone resorption and new bone formation which is regulated in part by some of the polypeptide growth factors such as platelet derived growth factor(PDGF), which has been known to be an important local regulator of bone cell activity and participate in normal bone remodeling. This process includes strictly regulated gene expression of several bone matrix proteins such as type I collagen and osteopontin, a 44 kDa phosphorylated glycoprotein, which has important roles in bone formation. The purpose of this study is to evaluate the effecs of PDGF-BB on the mRNA expression of bone matrix protein, type I collagen and osteopontin, in MC3T3- E1 cell culture. Cells were seeded at $5{\times}10^5$ cells in 10 ml of minimum essential medium alpha(${\alpha}-MEM$) containig 10% fetal bovine serum, 10 mM beta glycerophosphate. 0.1, 1, 10 ng/ml PDGF-BB were added to the cells for the day 3, 7, 14, 21, 28 and cultured for 24 hours. Type I collagen cDNA, Hf677, and osteopontin cDNA were used as probes for northern blot analysis. Total cellular RNA was purified at indicated day and northern blot analysis was performed. The results were as follows : Type I collagen mRNA expressions were higher at the day 3 and 7, and lower in the day 14, 21 in the control groups. In the experimental groups, mRNA expressions were increased when 0.1 ng/ml PDGF-BB were added on the day 3, 7, 21, and decreased in dose-dependent manner on the day 14, decreased at all added dose on the day 28. Osteopontin mRNA expressions were highest in the day 21 groups and lowest in the day 14 groups in the control groups. Interesting results were shown in the day 14 and 21 groups. We found that osteopontin mRNA level was increased in dose dependent manner in the day 14 groups, and decreased dose dependent manner in the day 21 groups. In conclusion, PDGF-BB may have various control effects on type I mRNA expression in the growth and differentiation process of MC3T3-E1 cells and may have contrary regulatory effects on osteopontin mRNA expression. For examples, when the baseline level of osteopontin mRNA was low, as in the day 14, PDGF-BB up-regulated osteopontin mRNA expression in dose dependent manner, and when the baseline level was high as in the day 21, PDGF-BB down-regulated dose dependent manner. Thus, it may be useful for clinical application in periodontal regeneration procedure if further study were performed.

• Journal of Korean Neurosurgical Society
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• v.59 no.6
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• pp.551-558
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• 2016

Malignant glioma cells invading surrounding normal brain are inoperable and resistant to radio- and chemotherapy, and eventually lead to tumor regrowth. Identification of genes related to motility is important for understanding the molecular biological behavior of invasive gliomas. According to our previous studies, Metallothionein 1E (MT1E) was identified to enhance migration of human malignant glioma cells. The purpose of this study was to confirm that MT1E could modulate glioma invasion in vivo. Firstly we established 2 cell lines; MTS23, overexpressed by MT1E complementary DNA construct and pV12 as control. The expression of matrix metalloproteinases (MMP)-2, -9 and a disintegrin and metalloproteinase 17 were increased in MTS23 compared with pV12. Furthermore it was confirmed that MT1E could modulate MMPs secretion and translocation of NFkB p50 and B-cell lymphoma-3 through small interfering ribonucleic acid knocked U87MG cells. Then MTS23 and pV12 were injected into intracranial region of 5 week old male nude mouse. After 4 weeks, for brain tissues of these two groups, histological analysis, and immunohistochemical stain of MMP-2, 9 and Nestin were performed. As results, the group injected with MTS23 showed irregular margin and tumor cells infiltrating the surrounding normal brain, while that of pV12 (control) had round and clear margin. And regrowth of tumor cells in MTS23 group was observed in another site apart from tumor cell inoculation. MT1E could enhance tumor proliferation and invasion of malignant glioma through regulation of activation and expression of MMPs.

• Journal of agriculture & life science
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• v.43 no.5
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• pp.9-15
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• 2009

The purpose of this study was to find out the optimum SMP carriers in seeds of 12 pepper cultivars to alternate Micro-eel E. It showed that calcium carbonate caused the lower germination rates in proportion of water volumes and the radicle protrusion during priming period. Micro-cel E induced also the radical protrusion, even though it shortened $T_{50}$ in Wang cultivar, in proportion of water volumes. Diatomaceous earth shortened both $T_{50}$ and Mean days germination(MDG) in Choyang and Hyangchon cultivars, although it caused the radicle protrusion in treatment of $1.5{\times}$ water proportion. In Wang cultivar, Diatomaceous earth shortened $T_{50}$ by 0.60 and 0.05 days at 1.0 and $1.5{\times}$ water proportions respectively compared to the Micro-cel E at 5.0 and $7.0{\times}$ water proportions. Therefore, it could be suggested that Diatomaceous earth for the carrier might be suitable in pepper cultivars to alternate Micro-cel E, and $1.0{\times}$ water proportion should be available for processing.

• Journal of Korean Dental Science
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• v.5 no.2
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• pp.77-87
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• 2012

Purpose: This study examined the scanning electron microscopic feature, protein marker expression and osteoinductive activity of demineralized dentin matrix (DDM) from human for nude mice. Materials and Methods: Twenty healthy nude mice, weighing about 20 g were used for study. DDM from Human was prepared and implanted into the dorsal portion of nude mouse. Before implantation, DDM was examined by scanning electron microscopy (SEM). Nude mice were sacrificed at 2 weeks, 4 weeks and 8 weeks after DDM grafting and evaluated histologically by H-E, MT staining. And also immunohistochemistry analysis (ostecalcin, osteopontin) was performed. Result: Dentinal tubules and collagen fibers were observed by SEM of dentin surface of DDM. The DDM induced bone and cartilage independently in soft tissues. And, the histological findings showed bone forming cells like osteoblasts, fibroblasts at 2, 4 and 8 weeks. On immunohistochemistry analysis, osteocalcin and osteopontin positive bone forming cells were observed. Conclusion: This results showed that the DDM from human has osteoinductive ability and is a good alternative to autogenous bone graft materials.

• 한국생물공학회:학술대회논문집
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• 2001.11a
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• pp.197-203
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• 2001

A fusion protein, consisting of human epidermal growth factor as a recognition domain and human angiogenin as a toxin domain, can be used as a targeted therapeutic against breast cancer cells among others. The fusion protein was expressed as inclusion body in recombinant E. coli, and when the conventional, solution-phase refolding process was used the refolding yield was very low due to severe aggregation, probably due to the opposite surface charge due to vastly different pI values of each domain. Solid-phase refolding process exploiting ionic interactions between the solid matrix and the protein was tried, but the ionic binding yield was very low regardless of the resins and pH conditions used. To provide higher affinity toward the solid matrix, six lysine residues were tagged to the N -terminus of the hEGF domain When the cation exchange resins such as heparin- or CM-Sepharose were used as the matrix, the adsorption capacity increased 2.5-3 times and the subsequent refolding yield increased nearly IS times compared to the conventional process.

• Journal of Korean Society for Quality Management
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• v.23 no.4
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• pp.28-41
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• 1995

We consider the problem of identifying multiple outliers in linear model. The available regression diagnostic methods often do not succeed in detecting multiple outliers because of the masking and swamping effect. Recently, among the various robust estimator of reducing the effect of outliers, LMS(Least Meadian Square) estimator has been to be a suitable method proposed to expose outliers and leverage points. However, as you know it, the data analysis method with LMS estimator is to be taken the median of the squared residuals in the sample which is extracted the sample space. Then this model causes the trouble, for the number of the chosen sample is nCp, i.e. as the size of sample space n is increasing, the number is increasing fastly. And the covariance matrix may be the singular matrix, so that matrix is approching collinearity. Thus we propose a procedure ELMS for the resampling in LMS method and study the size of the effective elementary set in this algorithm.

• Carbon letters
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• v.4 no.3
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• pp.111-116
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• 2003

Carbon/carbon composites were developed using PAN based carbon fibres and phenolic resin as matrix in different volume fractions and heat treated to temperatures between $1000^{\circ}C$ to $2500^{\circ}C$. Although both the starting precursors are nongraphitizing hard carbons individually, their composites lead to very interesting properties e.g. x-ray diffractograms show the development of graphitic phase for composites having fibre volume fractions of 30~40%. Consequently the electrical resistivity of such composites reaches a value of $0.8\;m{\Omega}cm$, very close to highly graphitic material. However, it was found that by increasing the fibre volume fraction to 50~60%, the trend is reversed. Optical microscopy of the composites also reveals the development of strong columnar type microstructure at the fibre (matrix interface due to stress graphitization of the matrix. The study forcasts a unique possibility of producing high thermal conductivity carbon/carbon composites starting with carbon fibres in the chopped form only.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.14 no.3
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• pp.236-243
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• 2004

In this paper a dynamic behavior of a simply supported cracked pipe conveying fluid with the moving mass is presented. Based on the Timoshenko beam theory, the equation of motion can be constructed by using the Lagrange's equation. The crack section is represented by a local flexibility matrix connecting two undamaged beam segments i.e. the crack is modelled as a rotational spring. This flexibility matrix defines the relationship between the displacements and forces across the crack section and is derived by applying fundamental fracture mechanics theory. And the crack is assumed to be in th first mode of fracture. As the depth of the crack and velocity of fluid are increased the mid-span deflection of the pipe conveying fluid with the moving mass is increased. As depth of the crack is increased, the effect of the velocity of the fluid on the mid-span deflection appears more greatly.

• 제어로봇시스템학회:학술대회논문집
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• 1994.10a
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• pp.364-368
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• 1994

Algebraic matrix Riccati equations of the form, FP+PF$^{T}$ -PRP+Q=0. are analyzed with reference to the stability of closed-loop system F-PR. Here F, R and Q are n * n real matrices with R=R$^{T}$ and Q=Q$^{T}$ .geq.0 (nonnegative-definite). Such equations have been playing key roles in optimal control and filtering problems with R .geq. 0. and also in the solutions of in H$_{\infty}$ control problems with R taking the form R=H$_{1}$$^{T}$ H$_{1}$-H$_{2}$$^{T}$ H$_{2}$. In both cases an existence of stabilizing solution, i.e. the solution yielding asymptotically stable closed-loop system, is an important problem. First, we briefly review the typical results when R is of definite form, namely either R .geq. 0 as in LQG problems or R .leq. 0. They constitute two extrence cases of Riccati to the cases H$_{2}$=0 and H$_{1}$=0. Necessary and sufficient conditions are shown for the existence of nonnegative-definite or positive-definite stabilizing solution. Secondly, we focus our attention on more general case where R is only assumed to be symmetric, which obviously includes the case for H$_{\infty}$ control problems. Here, necessary conditions are established for the existence of nonnegative-definite or positive-definite stabilizing solutions. The results are established by employing consistently the so-called algebraic method based on an eigenvalue problem of a Hamiltonian matrix.x.ix.x.

• Structural Engineering and Mechanics
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• v.21 no.5
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• pp.539-551
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• 2005

Finite elements based on isoparametric formulation are known to suffer spurious stiffness properties and corresponding stress oscillations, even when care is taken to ensure that completeness and continuity requirements are enforced. This occurs frequently when the physics of the problem requires multiple strain components to be defined. This kind of error, commonly known as locking, can be circumvented by using reduced integration techniques to evaluate the element stiffness matrices instead of the full integration that is mathematically prescribed. However, the reduced integration technique itself can have a further drawback - rank deficiency, which physically implies that spurious energy modes (e.g., hourglass modes) are introduced because of reduced integration. Such instability in an existing stiffness matrix is generally detected by means of an eigenvalue test. In this paper we show that a knowledge of the dimension of the solution space spanned by the column vectors of the strain-displacement matrix can be used to identify the instabilities arising in an element due to reduced/selective integration techniques a priori, without having to complete the element stiffness matrix formulation and then test for zero eigenvalues.