• Title/Summary/Keyword: e-matrix

Search Result 1,441, Processing Time 0.033 seconds

The Effects of Various Extracellular Matrices on Motility of Cultured MC3T3-E1 Cell (다양한 세포외기질이 배양 골아세포의 이동에 미치는 영향)

  • Park, Beyoung Yun;Seo, Sang Woo;Lee, Won Jai;Ryu, Chang Woo;Rah, Dong Kyun;Son, Hyun Joo;Park, Jong Chul
    • Archives of Plastic Surgery
    • /
    • v.32 no.2
    • /
    • pp.143-148
    • /
    • 2005
  • Chemotactic migration of bone forming cell, osteoblast, is an important event during bone formation, bone remodeling, and fracture healing. Migration of cells is mediated by adhesion receptors, such as integrins, that link the cell to extracellular matrix ligands, type I collagen, fibronectin, laminin and depend on interaction between integrin and extracellular ligand. Our study was designed to investigate the effect of extracellular matrix like fibronectin, laminin, type I collagen on migration of osteoblast. Migration distance and speed of MC3T3-E1 cell on extracellular matrix-coated glass were measured for 24 hours using 0.01% type I collagen, 0.01% fibronectin, 100 microliter/ml laminin. The migration distance and speed of MC3T3-E1 cell was compared using a video-microscopy system. To determine migration speed, cells were viewed with a 4 phase- contrast lens and video recorded. Images were captured using a color CCD camera and saved in 8-bit full-color mode. The migration distance on 0.01% type I collagen or 0.01% fibronectin was longer than that on $100{\mu}l/ml$ laminin-coated glass. The migration speed on fibronectin-coated glass was 68 micrometer/hour which was fastest. The migration speed on type I collagen-coated glass was similar with that on fibronectin-coated glass. The latter two migration speeds were faster than that on no-coated glass. On the other hand, the average migration speed on laminin-coated glass was 37micrometer/hour and not different from that of control group. In conclusion, the extracelluar matrix ligands such as type I collagen and fibronectin seem to play an important role in cell migration. The type I collagen or fibronectin coated scaffold is more effective for migration of osteoblast in tissue engineering process.

Temperature dependence of photocurrent for CdIn2Te4 single crystal grown by Bridgman method (Bridgman법으로 성장한 CdIn2Te4 단결정의 광전류 온도 의존성)

  • 유상하;홍광준
    • Proceedings of the Materials Research Society of Korea Conference
    • /
    • 2003.11a
    • /
    • pp.157-157
    • /
    • 2003
  • 수평 전기로에서 CdIn2Te4 다결정을 용융법으로 합성하고 Bridgman법으로 tetragonal structure의 c축에 평행한 CdIn2Te4 단결정을 성장시켰다. c축에 평행한 시료의 광흡수와 광전류 spectra를 293K에서 10K까지 측정하였다. 광흡수 spectra에 의해 band gap Eg(T)는 varshni공식에 따라 계산한 결과 1.4753eV-(7.78$\times$$10^{-3}$eV/K)T$^2$/(T+2155K)임을 확인하였다. Hall 효과는 van der Pauw 방법에 의해 측정되었으며, 온도에 의존하는 운반자 농도와 이동도는 293K에서 각각 9.01$\times$$10^{16}$ /㎤, 219 $\textrm{cm}^2$/V.S였다. 광전류 스펙트럼으로부터 Hamilton matrix(Hopfield quasicubic mode)법으로 계산한 결과 crystal field splitting $\Delta$cr값이 0.2704 eV이며 spin-orbit $\Delta$so 값은 0,1465 eV임을 확인하였다. 10K일 때 광전류 봉우리들은 n=1일때 Al-, Bl-와 Cl-exciton 봉우리임을 알았다.

  • PDF

A class of compact submanifolds with constant mean curvature

  • Jang, Changrim
    • Bulletin of the Korean Mathematical Society
    • /
    • v.34 no.2
    • /
    • pp.155-171
    • /
    • 1997
  • Let $M^n$ be a connected subminifold of a Euclidean space $E^m$, equipped with the induced metric. Denoty by $\Delta$ the Laplacian operator of $M^n$ and by x the position vector. A well-known T. Takahashi's theorem [13] says that $\delta x = \lambda x$ for some constant $\lambda$ if and only if $M^n$ is either minimal subminifold of $E^m$ or minimal submanifold in a hypersphere of $E^m$. In [9], O. Garay studied the hypersurfaces $M^n$ in $E^{n+1}$ satisfying $\delta x = Dx$, where D is a diagonal matrix, and he classified such hypersurfaces. Garay's condition can be seen as a generalization of T.

  • PDF

Removal of Cu(II) ions by Alginate/Carbon Nanotube/Maghemite Composite Magnetic Beads

  • Jeon, Son-Yeo;Yun, Ju-Mi;Lee, Young-Seak;Kim, Hyung-Il
    • Carbon letters
    • /
    • v.11 no.2
    • /
    • pp.117-121
    • /
    • 2010
  • The composites of alginate, carbon nanotube, and iron(III) oxide were prepared for the removal of heavy metal in aqueous pollutant. Both alginate and carbon nanotube were used as an adsorbent material and iron oxide was introduced for the easy recovery after removal of heavy metal to eliminate the secondary pollution. The morphology of composites was investigated by FE-SEM showing the carbon nanotubes coated with alginate and the iron oxide dispersed in the alginate matrix. The ferromagnetic properties of composites were shown by including iron(III) oxide additive. The copper ion removal was investigated with ICP AES. The copper ion removal efficiency increased greatly over 60% by using alginate-carbon nanotube composites.

A Design of All Metal Inserted E-Plane Waveguide Bandpass Filter Using GSM and Synthesis Design Method (GSM과 합성설계 방법을 이용한 E-평면형 마이크로파 도파관형 대역통과 여파기 설계)

  • 김영태;박준석;정명섭;천창율;김선형;임재봉
    • Proceedings of the Korea Electromagnetic Engineering Society Conference
    • /
    • 2002.11a
    • /
    • pp.173-176
    • /
    • 2002
  • An efficient design method for E-plane waveguide bandpass filter has been developed. The analysis method for E-plane discontinuities with finite length is based on Generalized Scattering Matrix (GSM) method. Furthermore, the design portion is based on the filter synthesis method. Waveguide bandpass filters designed with this method have been fabricated and measured. Excellent agreements with design have been obtained

  • PDF

For the quadrangular graphs (사변형 그래프에 관하여)

  • Jeong, Nae-Gyeong;Im, Hyung-Kyu;Park, Se-Won
    • The Journal of the Korea institute of electronic communication sciences
    • /
    • v.5 no.3
    • /
    • pp.239-244
    • /
    • 2010
  • In this paper, we study the adjacency matrix of a minimal connected quadrangular graph G, and then we obtain an upper bound on |E(G)| for such a graph G, and we obtain the graph for which the upper bound is attained. In addition, we obtain an upper bound on |E(G)| for a critical matching covered quadrangular graph G.

Enhancement of the solubility of human tissue inhibitor of matrix metallocroteinase-2 (TIMP-2) in E. coli using a modified in vitro mutagenesis (새로운 유전자 재조합 방법을 이용한 대장균에서의 인간 tissue inhibitor of mtrix metalloproteinase-2 (TIMP-2) 유전자의 가용성 발현)

  • Kim, Jong-Uk;Choi, Dong-Soon;Joo, Hyun;Min, Churl-K.
    • KSBB Journal
    • /
    • v.23 no.3
    • /
    • pp.231-238
    • /
    • 2008
  • The second family member of tissue inhibitors of matrix metalloproteinases, TIMP-2, is a 21kDa protein which inhibits matrix metalloproteinases 2 (MMP-2). Expression of mammalian proteins in E. coli often forms inclusion bodies that are made up of mis-folded or insoluble protein aggregates. The requirement for the formation of 6 disulfide bonds in the process of the TIMP-2 folding is likely to be incompatible with the reducing environment of E. coli. However, this incompatibility can be often overcome by introducing a mutagenesis that could lead to enhancement of the protein solubility. In this reason, we have attempted to express the soluble TIMP-2 in E. coli by applying a modified staggered extension process (StEP), one of the in vitro PCR-based recombinant mutagenesis methods, and error-prone PCR. C-terminally located CAT fusion protein with respect to mutated TIMP-2 proteins enables us to differentiate the soluble TIMP-2 from the insoluble in E. coli by virtue of chloramphenicol resistance. According to this scheme, E. coli harboring properly-folded CAT fused to TIMP-2 protein was selected, and some of the resulting colonies exhibited an enhanced, soluble expression of TIMP-2 compared to the wild type, implying (i) the StEP technique is successfully employed to enhance the proper folding thereby increasing the solubility of TIMP-2, and (ii) the CAT dependent screening may be a simple and effective method to differentiate the soluble protein expression in E. coli.

A FUNCTIONAL CENTRAL LIMIT THEOREM FOR MULTIVARIATE LINEAR PROCESS WITH POSITIVELY DEPENDENT RANDOM VECTORS

  • KO, MI-HWA;KIM, TAE-SUNG;KIM, HYUN-CHULL
    • Honam Mathematical Journal
    • /
    • v.27 no.2
    • /
    • pp.301-315
    • /
    • 2005
  • Let $\{A_u,\;u=0,\;1,\;2,\;{\cdots}\}$ be a sequence of coefficient matrices such that ${\sum}_{u=0}^{\infty}{\parallel}A_u{\parallel}<{\infty}$ and ${\sum}_{u=0}^{\infty}\;A_u{\neq}O_{m{\times}m}$, where for any $m{\times}m(m{\geq}1)$, matrix $A=(a_{ij})$, ${\parallel}A{\parallel}={\sum}_{i=1}^m{\sum}_{j=1}^m{\mid}a_{ij}{\mid}$ and $O_{m{\times}m}$ denotes the $m{\times}m$ zero matrix. In this paper, a functional central limit theorem is derived for a stationary m-dimensional linear process ${\mathbb{X}}_t$ of the form ${\mathbb{X}_t}={\sum}_{u=0}^{\infty}A_u{\mathbb{Z}_{t-u}}$, where $\{\mathbb{Z}_t,\;t=0,\;{\pm}1,\;{\pm}2,\;{\cdots}\}$ is a stationary sequence of linearly positive quadrant dependent m-dimensional random vectors with $E({\mathbb{Z}_t})={{\mathbb{O}}$ and $E{\parallel}{\mathbb{Z}_t}{\parallel}^2<{\infty}$.

  • PDF