• 대기
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• 제24권2호
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• pp.159-171
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• 2014

This study investigated the temporal and spatial characteristics of summertime (June-August) precipitation over Korean peninsula, using Korea Meteorological Administration (KMA)is Automated Synoptic Observing System (ASOS) data for the period of 1973-2010 and Automatic Weather System (AWS) data for the period of 1998-2010.The authors looked through climatological features of the summertime precipitation, then examined the degree of locality of the precipitation, and probable precipitation amount and its return period of 100 years (i.e., an extreme precipitation event). The amount of monthly total precipitation showed increasing trends for all the summer months during the investigated 38-year period. In particular, the increasing trends were more significant for the months of July and August. The increasing trend of July was seen to be more attributable to the increase of precipitation intensity than that of frequency, while the increasing trend of August was seen to be played more importantly by the increase of the precipitation frequency. The e-folding distance, which is calculated using the correlation of the precipitation at the reference station with those at all other stations, revealed that it is August that has the highest locality of hourly precipitation, indicating higher potential of localized heavy rainfall in August compared to other summer months. More localized precipitation was observed over the western parts of the Korean peninsula where terrain is relatively smooth. Using the 38-years long series of maximum daily and hourly precipitation as input for FARD2006 (Frequency Analysis of Rainfall Data Program 2006), it was revealed that precipitation events with either 360 mm $day^{-1}$ or 80 mm $h^{-1}$ can occur with the return period of 100 years over the Korean Peninsula.

• Ocean and Polar Research
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• 제25권1호
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• pp.63-74
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• 2003

The build-up of the heat field in shallow coastal water due to a point source has been investigated using an analytical solution of a time-integral form derived by extending the solutions by Holley(1969) and also presented in Harleman (1971). The uniform water depth is assumed with non-isotropic turbulent dispersion. The alongshore-flow is assumed to be uni-directional, spatially uniform and oscillatory. Due to the presence of the oscillatory alongshore-flow, the heat build-up occurs in an oscillatory manner, and the excess temperature thereby fluctuates in that course and even in the quasi-steady state. A series of calculations reveal that proper choices of the decay coefficient as well as dispersion coefficients are critical to the reliable prediction of the excess temperature field. The dispersion coefficients determine the absolute values of the excess temperature and characterize the shoreline profile, particularly within the tidal excursion distance, while the decay coefficient determines the absolute value of the excess temperature and the convergence rate to that of the quasi-steady state. Within the e-folding time scale $1/k_d$ (where $k_d$ is the heat decay coefficient), heat build-up occurs more than 90% of the quasi-steady state values in a region within a tidal excursion distance (L), while occurs increasingly less the farther we go to the downstream direction (about 80% at 1.25L, and 70% at 1.5L). Calculations with onshore and offshore discharges indicate that thermal spreading in the direction of the shoreline is reduced as the shoreline constraint which controls the lateral mixing is reduced. The importance of collecting long-term records of in situ meteorological conditions and clarifying the definition of the heat loss coefficient is addressed. Interactive use of analytical and numerical modeling is recommended as a desirable way to obtain a reliable estimate of the far-field excess temperature along with extensive field measurements.

• Journal of Microbiology
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• 제44권1호
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• pp.64-71
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• 2006

To investigate the effects of the nucleotide sequences in Shine-Dalgarno (SD) and the spacer region (SD-ATG) on bovine growth hormone (bGH) gene expression, the expression vectors under the control of the T7 promoter (pT7-7 vector) were constructed using bGH derivatives (bGH1 & bGH14) which have different 5'-coding regions and were induced in E. coli BL21 (DE3). Oligonucleotides containing random SD sequences and a spacer region were chemically synthesized and the distance between the SD region and the initiation codon were fixed to nine bases in length. The oligonucleotides were annealed and fused to the bGH1 and bGH14 cDNA, respectively. When the bGH gene was induced with IPTG in E. coli BL21(DE3), some clones containing only bGH14 cDNA produced considerable levels of bGH in the range of $6.9\%\;to\;8.5\%$ of total cell proteins by SDS-PAGE and Western blot. Otherwise, the bGH was not detected in any clones with bGH1 cDNA. Accordingly, the nucleotide sequences of SD and the spacer region affect on bGH expression indicates that the sequences sufficiently destabilize the mRNA secondary structure of the bGH14 gene. When the free energy was calculated from the transcription initiation site to the +51 nucleotide of bGH cDNA using a program of nucleic acid folding and hybridization prediction, the constructs with values below -26.3 kcal/mole (toward minus direction) were not expressed. The constructs with the original sequence of bGH cDNA also did not show any expression, regardless of the free energy values. Thus, the disruption of the mRNA secondary structure may be a major factor regulating bGH expression in the translation initiation process. Accordingly, the first stem-loop among two secondary structures present in the 5'-end region of the bGH gene should be disrupted for the effective expression of bGH.