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Anti-pruritic Effect of Ethanol Extracts from Platycodon grandiflorum and its fermented production in Scratching Behavior Mouse Models (길경(桔梗)발효 추출물의 알레르기성 소양행동 억제효과연구)

  • Ha, Mi-Ae;Kim, Jin-Woo;Lee, Shin-Woo;Chun, Hyun-Sik;Cho, Young-Son;Shin, Yong-Wook
    • The Korea Journal of Herbology
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    • v.29 no.6
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    • pp.165-173
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    • 2014
  • Objectives : Platycodon Root is frequently used in traditional medicine to treat inflammatory diseases of the throat. The purpose of this study was to characterize the effect of the EtOH extract of fermented Platycodon grandiflorum on the ameliorative effects on the Antipruritic Effect of atopic dermatitis mouse model induced by compound 48/80 and ovalbumin (OVA)-induced allergic responses in mice. Methods : In the present study, we examined the anti allergic effect of Platycodon grandiflorum (PR) and its fermented production (FPR) in several mouse model. We measured acute ear edema in a mouse model caused by TPA and consecutively histological change of Ear tissue was observed by hematoxylin and eosin (H&E) staining. and also Scratching behaviors by compound 48/80 was investigated. The levels of allergic mediators such as immunoglobulin (Ig) E, and anti-oxidant markers such as SOD and MDA in the sera of OVA induced allergic mice were measured by enzyme-linked immunosorbent assay. Results : FPR inhibited compoud 48/80-induced scratching behavior in mice, as well as acetic acid-induced writhing in mice. The anti-scratching behavioral effect of FPR was more potent than PR. FPR extract significantly decreased the serum levels of IgE and MDA compared with those of OVA control group. Conclusions : These results indicate that Anti allergic effect of Platycodon grandiflorum is enhanced by fermentation with Saccharomyces cerevisae and FPR may be useful for protection from the itching reactions, which are IgE-mediated representative skin allergic diseases.

Genetic Diversity and Genetic Structure of Acer pseudosieboldianum Populations in South Korea Based on AFLP Markers (AFLP 마커를 이용한 당단풍나무 집단의 유전다양성과 유전구조)

  • Ahn, Jiyoung;Hong, Kyung-Nak;Baek, Seung-Hoon;Lee, Min-Woo;Lim, Hyo-In;Lee, Jei-Wan
    • Journal of Korean Society of Forest Science
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    • v.105 no.4
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    • pp.414-421
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    • 2016
  • Fourteen Acer pseudosieboldianum populations in South Korea were used to estimate genetic diversity, genetic differentiation and genetic relationships using seven AFLP primer combinations. The average of effective alleles ($A_e$), the proportion of polymorphic loci (%P) and Shannon's diversity index (I) was 1.4, 82.2% and 0.358, respectively. The expected heterozygosity ($H_e$) under Hardy-Weinberg equilibrium was 0.231 and the expected heterozygosity (Hj) from Bayesian inference was 0.253. The level of genetic diversity was moderate compared to those of Genus Acer and lower than those of other species having similar ecological niche and life history. The inbreeding coefficient within populations ($F_{IS}$) from Bayesian method was 0.712 and it could be influenced by selfing or biparental inbreeding to induce homozygote excess. The level of genetic differentiation was 0.107 from AMOVA (${\Phi}_{ST}$) and 0.110 from Bayesian method (${\Phi}^{II}$). The genetic differentiation was lower than those of other species having similar ecological niche and life history. Ulleungdo population had the lowest level of genetic diversity and was genetically the most distinct population from others in the study. We consider that founder effect and genetic drift might be occurred to reduce genetic diversity and then the geographical isolation might interrupt gene flow to aggravate it.

Estrus synchronization and artificial insemination in Korean black goat (Capra hircus coreanae) using frozen-thawed semen

  • Kim, Kwan-Woo;Lee, Jinwook;Kim, Keun Jung;Lee, Eun-Do;Kim, Sung Woo;Lee, Sung-Soo;Lee, Sang-Hoon
    • Journal of Animal Science and Technology
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    • v.63 no.1
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    • pp.36-45
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    • 2021
  • Presently, there is an increased demand for livestock products all over the world which has led to more devotion on improving livestock population. Although goats have been bred for a long time in Korea, but there is not much research conducted on traditional Korean black goat (Capra hircus coreanae) compared to other livestock populations. Mutton consumption has been dramatically changing from medicinal use to edible meat and this trend directs the black goat populations declining and also mutton import quantities are increasing consistently. The present study introduced a new estrus synchronizing technique with subsequent artificial insemination (AI) for Korean black goats to enable crossbreeding with non-native breeds for the small or subsistent farmers. Our data highlighted that, the percentage of motile sperm from the electro-ejaculated samples declined significantly after freezing and melting. In addition, the sperm motility significantly declined with regard to sperm incubation period (0, 5, 60, and 120 min at 37℃) and was negatively correlated (64.2 ± 7.9%, 63.3 ± 5.8%, 49.9 ± 6.3%, and 35.9 ± 7.6%, respectively) in frozen-thawed sperm samples. Moreover, the E2 levels were unchanged even 24 h after controlled internal drug releas (CIDR) withdrawal. But, 48 h and 72 h after CIDR removal, E2 levels increased significantly. These data helps us to consider the two time points for AI; CIDR removal after 24 h, at which E2 decreases, and after 48 h, as the time at which progesterone increases. Additionally, the AI after 48 h of CIDR removal group exhibited significantly higher pregnancy and parturition rates (42.9%) compared to AI after 24 h after CIDR removal 28.6% group. In conclusion, these studies will propose an optimal estrus synchronisation process with subsequent timing of AI and also will promote the Korean black goat breeding industry.

Ectopic expression of soybean KS-type dehydrin, SLTI66 and SLTI629 conferred tolerance against osmotic and metal stresses of Escherichia coli and Arabidopsis

  • Chung, Eun-Sook;Cho, Chang-Woo;Kim, Kyoung-Mi;Lee, Jai-Heon
    • Journal of Plant Biotechnology
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    • v.36 no.1
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    • pp.38-44
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    • 2009
  • Two low temperature induced genes designated as SLTI66 and SLTI629 encoding KS-type dehydrin were heterologously expressed in E coli and A. thaliana. E coli cells expressing SLTI66 and SLTI629 protein grew better with iron stress compared to the control cells. Ectopic expression of SLTI629 conferred tolerance to iron stress in Arabidopsis but SLTI66 did not. Arabidopsis plants expressing SLTI66 showed enhanced tolerance to freezing and drought stress compared to those of wild type and SLTI629 lines. We propose that SLTI66 and SLTI629 play a different role as a protector against osmotic and metal stresses.

A Repository for Workflow Management on Distributed Object Environment (분산객체 환경에서의 워크플로우 관리를 위한 정보저장소)

  • Yeom, Tae-Jin;Park, Jae-Hyung;Ri, Ja;Kim, Ki-Bong;Jin, Sung-Il
    • The Journal of Society for e-Business Studies
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    • v.4 no.1
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    • pp.1-19
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    • 1999
  • Workflow management system provides automation of job processes by maintaining shareability on information about various job process schedules and persons related to those schedules. Existing workflow management systems use file or database to store the information generated in those systems. However, file or database system could manage only non-complicated information for the workflow but not the information resources of an enterprise which is complicated and of various formats. Therefore, we need a data management system that could control those information resources. This system should manage the data which are distributed at several places geographically. Information Repository could meet those requirements. Information Repository may integrate, store and manage information resources requested by application systems. We have an international standard for the information repository, Information Resources Dictionary System(IRDS). The IRDS, however, does not support distributed environment. In this paper, we design and implement an information repository based on IRDS that may be operated in distributed environment. We verify that this information repository is more effective and is more effective than any other file or database system.

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A P2P Based Tactical Information Sharing System for Mobile Nodes (P2P 기반의 모바일 노드간의 전술 정보 공유 시스템)

  • Lee, Hyun
    • Journal of the Korea Institute of Military Science and Technology
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    • v.17 no.4
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    • pp.501-509
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    • 2014
  • In NCW(Network Centric Warfare) environment, mobile nodes communicate through wireless link. But wireless link provides limited networking performance due to signal interferences or mobility of nodes. So it is quite challenge to acquire enough networking resources and use the resources efficiently. In this paper, we have proposed a P2P based tactical information sharing system which provides satisfactory visual information playout for mobile nodes(i.e., military personnel, vehicle,..) in NCW environment. Our proposed system consists of two components. One is caching-enabled switch which stores tactical information segments at its internal storage and then transports them to mobile nodes when require. Another is centralized scheduling algorithm which exploits networking resources more efficiently. To validate performance of proposed system, we performed series of experiments in wireless network testbed. Results show improved performance in terms of segment-missing ratio, networking resources usage, sharing time, and number of simultaneous playout mobile nodes with acceptable playout continuity(i.e., over 95%).

Collaborative Social Tagging for eBook using External DSL Approach

  • Yoo, Hwan-Soo;Kim, Seong-Whan
    • Proceedings of the Korea Information Processing Society Conference
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    • 2014.11a
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    • pp.1068-1072
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    • 2014
  • We propose a collaborative social tagging for eBook using external DSL approach. The goal of this paper is (1) to provide DSL by which authors can write HTML5 rich contents ebook and tag resources, (2) to make users enhance book by tagging resources easily, (3) to make readers read rich book easily regardless of their devices types, (4) to provide ebook resources of RESTful address style by which other system can identify self-descriptive resources of book. To achieve the goal, we provide Bukle DSL language by which author and users can author and enhance ebook with ease. As a domainspecific language Bukle provides a simple yet expressive language for authoring and tagging books that would otherwise be more difficult to express with a general purpose language. Further work includes visual DSL approach and tools by using that the unskilled users could tag book easily. In order that future work also includes text-to-visual DSL transform engine. UX research is also required to tag and to author book. To tackle the above questions we are looking at using visual notation focusing visual syntax.

Information Networking and its Application in the Digital Era with Illustration from the University of Port Harcourt Library

  • Umeozor, Susan Nnadozie
    • International Journal of Knowledge Content Development & Technology
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    • v.9 no.2
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    • pp.33-44
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    • 2019
  • This paper discussed the factors that necessitated information networking, types of networks, benefits of information networking, library information networking and the University of Port 0Harcourt library network initiatives. Information networking is a process of communication, exchange of ideas, resource sharing and collaboration between individuals, organizations, institutions and libraries and it is facilitated by ICTs and the internet for improved accessibility. It has been brought about by information explosion, rapid advancement in information communication technologies, inadequate funding and increased demand for quality information. Networks can be classified into local, national, regional, and international networks and are formed to serve different categories of user communities. Benefits of information networking include resource sharing, on-line conferences and participation in programmes at distant centers, collaboration among scholars in different countries. Communication flow through the internet, social media, and electronic mail. Library information networking started with the interlibrary loan which has metamorphosed into library consortia in which groups of libraries partner to coordinate activities, share resources and combine expertise. The University of Port Harcourt Library network initiatives started with an e-granary (a CD ROM) and the establishment of a local area network. The library subscribes to more than 10 electronic databases. Information networking has greatly improved the sharing of resources in acquisition and dissemination of information resources since no single institution can acquire the overwhelming number of information resources in their various formats.