• Molecules and Cells
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• v.26 no.1
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• pp.41-47
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• 2008

Mitogen-activated protein kinase (MAPK) signaling is a crucial component of eukaryotic cells; it plays an important role in responses to extracelluar stimuli and in the regulation of various cellular activities. The signaling cascade is evolutionarily conserved in the eukaryotic kingdom from yeast to human. In response to a variety of extracellular signals, MAPK activity is known to be regulated via phosphorylation of a conserved $T{\times}Y$ motif at the activation loop in which both threonine and tyrosine residues are phosphorylated by the upstream kinase. However, the mechanism by which both residues are phosphorylated continues to remain elusive. In the budding yeast, Saccharomyces cerevisiae, Fus3 MAPK is involved in the mating signaling pathway. In order to elucidate the functional mechanism of MAPK activation, we quantitatively profiled phosphorylation of the $T{\times}Y$ motif in Fus3 using mass spectrometry (MS). We used synthetic heavy stable isotope-labeled phosphopeptides and nonphosphopeptides corresponding to the proteolytic $T{\times}Y$ motif of Fus3 and accompanying data-dependent tandem MS to quantitatively monitor dynamic changes in the phosphorylation events of MAPK. Phosphospecific immunoblotting and the MS data suggested that the tyrosine residue is dynamically phosphorylated upon stimulation and that this leads to dual phosphorylation. In contrast, the magnitude of threonine phosphorylation did not change significantly. However, the absence of a threonine residue leads to hyperphosphorylation of the tyrosine residue in the unstimulated condition, suggesting that the threonine residue contributes to the control of signaling noise.

• Journal of the Ergonomics Society of Korea
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• v.24 no.1
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• pp.9-17
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• 2005

81.1% of traffic accidents is attributed to the drivers. In this regard, D/E model is a practical and effective method in terms of the cost and time in evaluating the road hazardousness. To examine the validity of the threshold values by the levels of demand We selected 10 subjects and collected their physiological signals while they were driving on Honam Highway (Jeonju ${\leftrighttarro}$ Hoideog section). Based on the collected data, the hazardous road condition was evaluated using the new threshold values of the effort level determined by cluster analysis. In applying the D/E model, a decision method based on the demand level was suggested, using a traffic accident prediction model. Additionally, the limit value of the effort level was determined using the drivers' physiological signal data collected at the highway. A comparison analysis of the two D/E models revealed no significant difference: The existing method and the clustering method determined 9 and 7 hazardous road zones, respectively, while actual traffic accidents were reported in 6 and 4 zones, respectively among the predicted road hazardous zones. However, the latter method suggested a more scientific and rational basis in determining the limit value of the Effort level. In conclusion, although D/E model has a great merit as a pioneering method to reflect human factors in evaluating the road hazardousness, it is believed that this method could be improved by a more dynamic method that considers the traffic conditions and the individual physiological signal of the drivers simultaneously in determining a better limit.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.42 no.9 s.339
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• pp.49-56
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• 2005

Memory effect, which influence the performance of Doherty amplifier, become more significant and critical in designing these circuits as the modulation signal bandwidth and operation power level increase. This paper reports on an attempt to investigate, model and quantity the contribution of the electrical nonlinearity effects and the thermal memory effects to a Doherty amplifier's distortion generation. Also this raper reports on the development of an accurate dynamic expression of the instantaneous junction temperature as a function of the instantaneous dissipated power. This expression has been used in the construction of an electrothermal model for the Doherty amplifier. Parameters for the nelv proposed behavior model were determined from the Doherty amplifier measurements obtained under different excitation conditions. This study led us to conclude that the effects of the transistor self-heating phenomenon are important for signals with wideband modulation bandwidth(ex. W-CDMA or UMTS signal). Doherty amplifier with electrothermal memory effect compensator enhanced ACLR performance about 20 dB than without electrothemal memory effect compensator. Experiment results were mesured by 60W LDMOS Doherty amplifier and electrothermal memory effect compensator was simulated by ADS.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.11 no.1
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• pp.84-92
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• 2000

A GaAs MESFET MMIC transmitter for 2.4 GHz wireless local loop handset is designed and fabricated. The transmitter consists of a double balanced active mixer and a two stage driver amplifier with voltage negative feedback. In particular, a pair of CS-CG(common source-common gate) structure compensates the reduction in dynamic range caused by unbalanced complementary IF input signals. And to suppress the leakage local power at RF port, the mixer is designed by using phase characteristic between the ports of MESFET. At the bias condition of 2.7 V and 55.2 mA, the fabricated MMIC transmitter with chip dimensions of $0.75\times1.75 mm^2$ obtains a measured conversion gain of 38.6 dB, output $P_{idB}$ of 11.6 dBm, and IMD3 at -5 dBm RF output power of -31.3 dBc. This transmitter is well suited for WLL handset.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.18 no.7
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• pp.1589-1598
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• 2014

To support the WBAN, IEEE 802.15 Task Group 6 announced standardized documents on technical requirements of the PHY and MAC. In the IEEE 802.15.6 MAC protocol, CSMA/CA(Carrier Sense Multiple Access with Collision Avoidance) algorithm is performed based on the eight-level priorities according to the type of traffics of the periodic data from medical sensor nodes. Several nodes, which detected the changed bio signals, transmit emergency data at the same time, so latency could be higher than emergency latency and energy consumption will increase. In this thesis, we proposed a CSMA/CA algorithm in WBAN to solve these problems. Simulations are performed using a Castalia based on the OMNeT++ network simulation framework to estimate the performance of the proposed superframe and algorithms. Performance evaluation results show that the packet transmission success rate and energy efficiency are improved by reducing the probability of collision using the proposed MAC protocol.

• Transactions of the Korean Society of Machine Tool Engineers
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• v.11 no.6
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• pp.75-82
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• 2002

Design for a quiet operation of fluid power system requires the understanding of noise and vibration characteristics of the system. It's not easy to analyze noise problem in hydraulic cylinder used in typical actuator Because they've got complex fluid dynamics. One of the fundamental problems associated with the hydraulic system is the pulsating flow in pipe lines, which can be tackled by the analysis under simplifying assumptions. The present study focuses on theoretic analysis and experimental study on the dynamics of laminar pulsating flow in a circular pipe. We analyze the propagation characteristics of the pressure pulse within a hydraulic pipe line taking into account the pulsating flow frequency variation. We also measure instantaneous pressure pulses within pipe line to identify the transfer functions. We conduct series of experiments to investigate the propagation characteristics of pressure pulse for various pressure of pulsating flow. The working fluid of the present study is ISO VG46 and the temperature ranges from 20 to $60^{\circ}$ with normal pressure at 4000kPa. The flow rate is measured by using an ultrasonic flow meter. Pressures at fixed upstream and downstream positions are measured concurrently. The electric signals of the pressure sensor are stored and analyzed using a system analyzer(PKE 983 series). The frequency is varied in the range of 10~500Hz. The Reynolds number is kept below 2,000. In the present study, boundary condition was varied by installing a surge tank and an orifice at the end of pipe. Experimental and theoretical results were compared each other under various boundary conditions.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.40 no.9
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• pp.783-790
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• 2016

One of the main factors related to the deterioration of estimation accuracy in inertial measurement unit (IMU)-based orientation determination is the object's acceleration. This is because accelerometer signals under accelerated motion conditions cannot be longer reference vectors along the vertical axis. In order to deal with this issue, some orientation estimation algorithms adopt acceleration-compensating mechanisms. Such mechanisms include the simple switching techniques, mechanisms with adaptive estimation of acceleration, and acceleration model-based mechanisms. This paper compares these three mechanisms in terms of estimation accuracy. From experimental results under accelerated dynamic conditions, the following can be concluded. (1) A compensating mechanism is essential for an estimation algorithm to maintain accuracy under accelerated conditions. (2) Although the simple switching mechanism is effective to some extent, the other two mechanisms showed much higher accuracies, particularly when test conditions were severe.

• Journal of Institute of Control, Robotics and Systems
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• v.22 no.4
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• pp.293-300
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• 2016

The Voice recognition is one of convenient methods to communicate between human and robots. This study proposes a speech recognition method using speech recognizers based on Hidden Markov Model (HMM) with a combination of techniques to enhance a biped robot control. In the past, Artificial Neural Networks (ANN) and Dynamic Time Wrapping (DTW) were used, however, currently they are less commonly applied to speech recognition systems. This Research confirms that the HMM, an accepted high-performance technique, can be successfully employed to model speech signals. High recognition accuracy can be obtained by using HMMs. Apart from speech modeling techniques, multiple feature extraction methods have been studied to find speech stresses caused by emotions and the environment to improve speech recognition rates. The procedure consisted of 2 parts: one is recognizing robot commands using multiple HMM recognizers, and the other is sending recognized commands to control a robot. In this paper, a practical voice recognition system which can recognize a lot of task commands is proposed. The proposed system consists of a general purpose microprocessor and a useful voice recognition processor which can recognize a limited number of voice patterns. By simulation and experiment, it was illustrated the reliability of voice recognition rates for application of the manufacturing process.

• The Korean Journal of Physiology
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• v.30 no.1
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• pp.85-96
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• 1996

Adenosine and its analogues are known to possess analgesic effects and to be involved in the opiate-induced antinociception as well. This study was designed to investigate the effects of three adenosine agonists, 5'- (N-cyclopropyl) -carboxamidoadenosine(CPCA), 5'-N-ethylcarboxamidoadeno-sine (NECA) and $N^6-cyclohexyladenosine$ (CHA) on the signal transmission in the spinal cord and also to elucidate mechanisms of their actions in the anesthetized cat. All the tested adenosine agonists(i.v,) exerted inhibitory effects on the responsiveness of the wide dynamic range (WDR) cells, the inhibitory action of CHA, an adenosine $A_1$ receptor agonist, $(80{\mu}g/Kg)$ being most weak. The intravenous CPCA, an adenosine $A_2$ receptor agonist, $(20{\mu}g\;/Kg)$ and NECA, nonspecific adenosine receptor agonist, $(20{\mu}g\;/Kg)$ inhibited the responses of WDR cells to pinch and C fiber stimulation more strongly than those to brush and A fiber stimulation. CPCA (i.v.) also suppressed the responses of WDR cells to thermal stimulus. And all the CPCA-induced inhibitions were caffeine-reversible. When CPCA was directly applied onto the spinal cord or intravenously administered into the spinal cat, on average, about three quarters of the CPCA-induced inhibitory effect was abolished. On the other hand, in the animal with spinal lesions in the ipsilateral dorsolateral area, the CPCA-induced inhibition was comparable to that observed in the spinal cats. In conclusion, this study shows that adenosine agonists strongly suppress the responses of WDR cells to pinch, C fiber stimulation and thermal stimuli mainly through the supraspinal adenosine $A_2-receptors$.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.33 no.3
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• pp.231-238
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• 2020

This paper investigates the soundness of porcelain insulators associated with the acoustic emission (AE) technique. The AE technique is a popular non-destructive method that measures and analyzes the burst energy that occurs mainly when a crack occurs in a high-frequency region. Typical AE methods require continuous monitoring with frequent sensor calibration. However, in this study, the AE technique excites a porcelain insulator using only an impact hammer, and it applies a high-pass filter to the signal frequency range measured only in the AE sensor by comparing the AE and the acceleration sensors. Next, the extracted time-domain signal is analyzed for the damage assessment. In normal signals, the duration is about 2ms, the area of the envelope is about 1,000, and the number of counts is about 20. In the damage signal, the duration exceeds 5ms, the area of the envelope is about 2,000, and the number of counts exceeds 40. In addition, various characteristics in the time and frequency domain for normal and damage cases are analyzed using the short-time Fourier transform (STFT). Based on the results of the STFT analysis, the maximum energy of a normal specimen is less than 0.02, while in the case of the damage specimen, it exceeds 0.02. The extracted high-frequency components can present dynamic behavior of crack regions and eigenmodes of the isolated insulator parts, but the presence, size, and distribution of cracks can be predicted indirectly. In this regard, the characteristics of the surface crack region were derived in this study.