• Korean Journal of Environmental Agriculture
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• v.28 no.3
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• pp.238-242
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• 2009

The effect of earthworm and dung beetle on cattle dung pat decomposition was assessed by combining quantification of earthworm density and with or without dung beetle in pats and measurements of the decomposition rate of these pats. Cattle dung decomposition rate was higher in the pots treated with both earthworm and dung beetle than in the pots with either earthworm or beetle alone. After dung beetle and earthworm activity, the growth of oat in earthworm with dung beetle treatment was similar effect with fertilizer treatment. Dung beetle was responsible for dung decomposition until 78% moisture content in the dung, earthworm was responsible for up to 30% moisture of dung, and two group were not shown any activity for decomposition less 30% moisture content of dung. Therefore dung in the different periods could be broken down by each group. The disappearance and conveyance of dung by earthworm and dung beetle was 72% of the initial dung amount. 10.2% of 72% dung was used making brood balls by dung beetle. Earthworm activity was not an impediment on making brood balls by dung beetles. The interaction of earthworm and dung beetle may have a complementary cooperation rather than competition in the same dung pat. Indeed, development of earthworm accelerate to coexist with dung beetles instead without dung beetles. From this result, maximum benefits of the effective earthworm and dung beetle can be achieved, it is needed to preserve population of earthworm and dung beetles in pasture to sustainable agricultural environment.

• Parasites, Hosts and Diseases
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• v.47 no.1
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• pp.13-17
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• 2009

Dung beetles (family Scarabaeidae) are one of the largest families of beetles worldwide. Due to biological behavior of these arthropods, they are considered to play an important role in the life cycle of some helminths. In the present study, dung beetles collected from cattle pastures in rural areas of Ardabil province, north-west of Iran were examined for infection with larval stages of helminths. According to the results, nematodes of 2 genera were identified including Rhabditis and Gongylonema. The more common species was Rhabditis sp. which was found in 9 species of beetles. Out of 15 different species of dung beetles, Copris lunaris was the only scarabaeid to be found naturally infected with the larval stages of Gongylonema sp. Our new findings introduce C. lunaris as a potential biological vector for transmission of Gongylonema sp. to vertebrates in the surveyed region.

• Journal of The Korean Society of Grassland and Forage Science
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• v.25 no.1
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• pp.51-56
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• 2005

The dung beetle species living in grazing pasture in Korea and their life cycle such as characteristics of habitation and hibernation were investigated for five year. Eleven species belonging to five genera of dung beetles were found in the grazing pasture. They started to appear around the middle of April when grazing begins on pasture. Dung booties kept on laying eggs until the beginning of August and maintained their activity until the end of October. They passed the winter as a form of an imago twenty five to thirty centimeters under the ground. Loamy soil and sandy soil containing plenty of humus were prefered as a hibemaculum by them. Five genera of dung beetles. Aprodius spp, Onthophagus spp., Liatongus spp., Copris spp., Scarabaeus spp. were found and observed in s study. Three species of them like Scarabaeus affinis had more an twenty eight millimeter long body, and the body length of five species like Copris tripartitus were between ten and twenty millimeters. Three species of them like Apodius sublimbatus were had the body length of less than ten millimeters. The results of indoor experiments to study propagation power of dung beetle showed that the optimum temperature for propagation of Copris ochus and Copris tripartitus were between twenty and thirty degrees and the lowest temperature for living of dung beetle was eighteen degrees while e highest temperature being thirty five degrees. A light did not effect the propagation power of dung beetles.

• Journal of Animal Science and Technology
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• v.63 no.1
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• pp.16-24
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• 2021

The suppressive effect of monensin as an ionophore-feed additive on enteric methane (CH4) emission and renewable methanogenesis were evaluated. To clarify the suppressive effect of monensin a respiratory trial with head cage was performed using Holstein-Friesian steers. Steers were offered high concentrate diets (80% concentrate and 20% hay) ad libitum with or without monensin, galacto-oligosaccharides (GOS) or L-cysteine. Steers that received monensin containing diet had significantly (p < 0.01) lower enteric CH4 emissions as well as those that received GOS containing diet (p < 0.05) compared to steers fed control diets. Thermophilic digesters at 55℃ that received manure from steers fed on monensin diets had a delay in the initial CH4 production. Monensin is a strong inhibitor of enteric methanogenesis, but has a negative impact on biogas energy production at short retention times. Effects of the activity of coprophagous insects on CH4 and nitrous oxide (N2O) emissions from cattle dung pats were assessed in anaerobic in vitro continuous gas quantification system modified to aerobic quantification device. The CH4 emission from dungs with adults of Caccobius jessoensis Harold (dung beetle) and the larvae of the fly Neomyia cornicina (Fabricius) were compared with that from control dung without insect. The cumulative CH4 emission rate from dung with dung insects decreased at 42.2% in dung beetles and 77.8% in fly larvae compared to that from control dung without insects. However, the cumulative N2O emission rate increased 23.4% in dung beetles even though it reduced 88.6% in fly larvae compared to dung without coprophagous insects. It was suggested that the antibacterial efficacy of ionophores supplemented as a growth promoter still continued even in the digested slurry, consequently, possible environmental contamination with the antibiotics might be active to put the negative impact to land ecosystem involved in greenhouse gas mitigation when the digested slurry was applied to the fields as liquid manure.

• Journal of Species Research
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• v.6 no.2
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• pp.181-189
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• 2017

Sixteen species of macrochelid mites were collected on the surface of scarab dung beetles in Mount Gede-Pangrango National Park, West Java, Indonesia. Of these, one species, Macrocheles gedeensis sp. nov. is described here as new to science. The other fifteen species were Holostaspella katakurai Hartini and Takaku, 2003, H. oblonga Hartini and Takaku, 2010, H. villosa Hartini and Takaku, 2010, Neopodocinum halimunense Hartini and Takaku, 2003, N. subjaspersi Hartini and Takaku, 2003, Neopodocinum sp., Macrocheles dispar (Berlese, 1910), M. entetiensis Hartini and Takaku, 2005, M. hallidayi Walter and Krantz, 1986, M. jabarensis Hartini and Takaku, 2003, M. kraepelini (Berlese, 1905), M. pumilus Hartini, Dwibadra and Takaku, 2009, M. turgoensis Hartini, Dwibadra and Takaku, 2009, M. sukabumiensis Hartini and Takaku, 2003, and M. sukaramiensis Takaku, 2001.

• Archives of Pharmacal Research
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• v.28 no.7
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• pp.816-822
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• 2005

Catharsius protease-2 (CPM-2) was isolated from the body of dung beetles, Catharsius molossus, using a three step purification process (ammonium sulfate fractionation, gel filtration on Bio-Gel P-60, and affinity chromatography on DEAE Affi-Gel blue). The purified CPM-2, having a molecular weight of 24 kDa, was assessed homogeneously by SDS-polyacrylamide gel electrophoresis. The N-terminal amino acid sequence of CPM-2 was composed of X Val Gin Asp Phe Val Glu Glu lie Leu. CPM-2 was inactivated by $Cu^{2+}\;and\;Zn^{2+}$ and strongly inhibited by typical serine proteinase inhibitors such as TLCK, soybean trypsin inhibitor, aprotinin, benzamidine, and ${\alpha}_1$-antitrypsin. However, EDTA, EGTA, cysteine, $\beta$-mercaptoethanol, E64, and elastatinal had little effect on enzyme activity. In addition, antiplasmin and antithrombin III were not sensitive to CPM-2. Based on the results of a fibrinolytic activity test, CPM-2 readily cleaved $A{\alpha}-$ and $B{\beta}$-chains of fibrinogen and fibrin, and y-chain of fibrinogen more slowly. The nonspecific action of the enzyme resulted in extensive hydrolysis, releasing a variety of fibrinopeptides of fibrinogen and fibrin. Polyclonal antibodies of CPM-2 were reactive to the native form of antigen. The ELISA was applied to detect quantities, in nanograms, of the antigen in CPM-2 protein.

• Journal of Species Research
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• v.13 no.3
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• pp.306-309
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• 2024

Rhyssemus Mulsant, 1842, represents one of the largest genera in the tribe Psammodiini, comprising approximately 170 species to date. This group is nearly cosmopolitan, excluding the Neotropical region, with 59 species recorded in the Palearctic region. On the Korean Peninsula, only one species, Rhyssemus koreanus Stebnicka, 1980 was recorded from North Korea. In this study, the genus Rhyssemus is recorded for the first time with a newly recorded species Rhyssemus inscitus (Walker, 1858) from South Korea. Adult specimens were collected from the costal sand dunes in Taean-gun and Jeju island. We herein provide a diagnosis, illustrations of morphological characters, and habitat information. Partial mitochondrial COI sequences of the species are also provided for DNA barcoding.