• Applied Biological Chemistry
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• 제33권2호
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• pp.169-173
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• 1990

곡류 등에 오염되어 황색 독소성분인 skyrin을 생합성하는 Penicillium islandicum 배지에 grapefruit 종자로부터 추출한 천연유기 복합물인 DF-100을 처리하여 곰팡이 생육 및 skyrin 생성을 저해하는 효과를 볼 수 있었다. DF-100 500ppm 농도로 Penicillium islandicum의 생육을 91% 저해하였고 750ppm 농도에서 곰팡이 생육을 완전히 저해하였으며 500ppm농도로 skyrin생성을 완전히 저해하였다. skyrin 생합성경로를 고려할 때 DF-100은 낮은 농도에서 emodinanthrone으로부터 emodin을 거쳐 skyrin으로 전환되는 효소 반응계와 skyrinanthrone으로 진행하는 반응단계를 저해하는 것으로 밝혀 졌다.

• 약학회지
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• 제17권3호
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• pp.129-136
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• 1973

In order to establish the chemical standards for the quality control of ginsentgextract, an approach for the assay of sapogenin contents in the part of main roots and fibrous side roots was performed by combination of preparative thin layer chromotographic procedure and vanillin-$H_{2}SO_{4}$ color reaction. The contents of dammarane aglycones as funcction of dammarane glycosides in 80%-EtOH extracts were analyzed by the method from the main roots and fibrous side roots of Korean ginseng grown for 4-6 years. The differences by their grown ages in the contents of dammarane glycosides, in the ratio of panaxadiol to panaxatriol contents, and in the mounts of 80% EtOH extract were not significant in the parts of main roots and fibrous side roots of Korean gingeng. Differences due to the part for medicinal uses were highly significant in all parameters mentioned, showing following results ; in the main roots ; 80% EtOH extract, 12.7-15.7 % : the ratio of aglycone composition, 0.955-1.012 : dammarane glycoside (as diglucoside bases), 1.537-1.863 ; in fibrous isde roots ; 80% EtOH extract, 26.0-26.02% : dammarane glycoside, 4.767-5.641 : the ratio, 1.456-1.50.

• 한국식품영양과학회지
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• 제35권3호
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• pp.353-358
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• 2006

유자씨앗 중의 플라보노이드 및 리모노이드 화합물을 methanol 및 ethanol로 추출 HPLC로 정량한 결과 ethanol추출의 경우가 거의 모든 화합물에서 높게 나타났고 화합물의 종류도 더 많이 검출되었다. Limonin이 methanol 추출의 경우 140.34 mg/100g, ethanol 추출의 경우 170.98 mg/100g으로 개별화합물 중 가장 높은 함량을 나타내었고 그다음이 caffeic acid, naringin, lutin, nomilin 등의 순으로 높았다. HPLC/MS에 의하여 약42가지 화합물의 분자량 측정이 가능하였으며 mass spectrum과 extracted current ion chromatogram으로 분자량 측정이 가능하였다.

• 한국식품영양과학회지
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• 제27권5호
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• pp.821-826
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• 1998

Functional properties of mucilage and pigment extracted from Opuntia ficus-indica var. saboten were determined at various temperatures, pHs and alcohol concentrations. The crude mucilage extracted from pickly pear showed pH 4.2, 0.14% total acidity and 8.1% total soluble solid content(w/w, wet basis). Polysaccharide was purified from mucilage extract by isopropanol precipitation. Intrinsic viscosity of polysaccharide was 18.1dl/g and decreased with increasing KCl concentration. Relative viscosity and color stability of mucilage extract were determined with capillary viscometer and spectrophotometer at 534nm, respectively. In additions of 1~20%(v/v) ethanol, the red pigment of mucilage extract was very stable, but relative viscosity, increased gradually. For heating above 7$0^{\circ}C$, the stability of red pigment decreased drastically, but rheological property of mucilage was not changed. During storage, the red pigment was extremely unstable at above pH 8.3. At both pH 3.0 and pH 4.2, the red pigment was the most stable at 4$^{\circ}C$ for 18 days. In the case of storage at 37$^{\circ}C$, pigment of mucilage extract at pH 3.0 was destroyed more quickly than that at pH 4.2. Natural mucilage extract(pH 4.2) showed the good stability of red pigment at 3$0^{\circ}C$ for 10 days.

• 한국식품영양과학회지
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• 제24권6호
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• pp.874-879
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• 1995

본 연구는 오리고기로 부터 추출한 오리기름을 사용하여 성숙(52주령)된 흰주의 혈청과 장기의 지질 함량에 미치는 영향을 조사하고자 대조군으로 식물성 유지인 대두유군, 동물성 유지인 오리기름군, 오리기름에 1% 콜레스테롤을 첨가한군 등 3구능로 나누터 각각 8주간 사육한 후 각군의 혈청과 장기의 지질 농도를 측정한 결과는 다음과 같다. 혈정 중 중성지방 함량은 대조구넹 비하여 오름기름군, 오리기름에 1% 콜레스테롤을 첨가한 군에서 유의적으로 낮았으며(p<0.05), 인지질 함량은 차이가 없었다. 혈청 중 콜레스테롤 함량은 대조군과 오리기름군에 비하여 오리기름에 1% 콜레스테롤을 첨가한 군에 유의하게 높았으나(p<0.05), HDL - 콜레스테롤 함량은 유의하게 낮았다(p<0.05). HDL - cholesterol/Total-cholesterol의 비율은 대조군에 비하여 오리기름군과 오리기름에 1% 콜레스테롤을 첨가한 군이 각각 유의적으로 (p<0.05) 오리기름에 1% 콜레스테롤을 첨가한 군이 더 낮게 나타났다(p<0.05). 장기의 지질 함량에서 간의 총 지질, 중성지질 및 총콜레스테롤 함량은 대조군과 오리기름군에 비하여 오리기름이 1% 콜레스테롤을 첨가한 군이 유의하게 높았으며(p< 0.05), 대조군과 오리기름군은 차이가 없엇고, 인지질 함량은 세군 모두 차이가 없었다. 이상의 결과로 보아 오리기름이 혈청과 가의 중성지질은 대두유에 비하여 감소시키는 효과가 있으나 총 콜레스테롤 농도는 대두유군과 비슷한 수준을 보였다. 그러나 오리기름에 1% 콜레스테롤을 첨가군에서는 hypercholester rolemic 개선 효과는 나타나지 않았다.

• 사상체질의학회지
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• 제9권2호
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• pp.203-225
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• 1997

Jihwangbakhotang(地黃白虎楊) is made by Li Je Ma, the creator of the Four Constitutional Medicine. Single and 13 weeks oral repeated dose toxicity studies were conducted in Sprague Dawley rats of both sexes to elucidate the potential acute and subchronic toxicity of JBT extract and reversibility of any effects. In the single dose study, JBT extract was administered orally to rats with the dose of 2 g/kg and 8 g/kg. In the long term administration of 13 weeks, the JBT extract of 125 mg/kg/day, 500 mg/kg/day, 2000 mg/kg/day was administered to rats. The change of blood weight, urine volume, electrolyte in urine, hematological change, the change of blood chemistry, autopsy finding, and histological observation were researched, the results were as follows; 1. The lethal dose of JBT extract seems to be over 10 g/kg, the single administration of JBT extract 8 g/kg showed no toxical signs except little increase of urine volume. 2. The change of body weight had the trend of decrease in the group of, but has no significance, and also the consumption of food and water had no changes. 3. The hematological changes induced by the 13 weeks administration of JBT extract showed the significance in the item of Hb, MCH, MCV, WBC in the group of 125 mg/kg/day. 4. In the test of blood chemistry, total cholesterol showed little decrease and A/G ratio showed little increase, but the change was not clear, and the standard error was large. So the result was obtained insignificantly and the toxicity of JBT extract was not observed. 5. In the male group after recovery period, the level of cholesterol and triglyceride decreased slightly, but the result was not significant. 6. In the urine test, the little change of electrolyte was appeared, but it seemed not to be the result induced by the toxicity of JBT extract. 7. In each group of male and female rats, the weight change of organ and the serum histological changes was observed, but the result did not showed the dose dependent toxicity. So the toxicity of JBT extract was not regarded. In the conclusion, the toxicity of JBT extract was not observed in the single dose treatment and long term repetitive administration of JBT extract.

• 생약학회지
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• 제16권4호
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• pp.181-190
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• 1985

To determine contents of inorganic elements of Ganoderma lucidum, the horn-shaped carpophores and the pileus of Ganoderma lucidum were incinerated and analyzed by inductively coupled plasma atomic emission spectrophotometry. The ash contents of the pileus and the horn-shaped carpophore were 1.48% and 1.40%, respectively. The pileus contained calcium, magnesium, sodium, manganese, iron, zinc and germanium in that order. The horn-shaped carpophore contained magnesium, calcium, zinc, manganese, iron, copper and germanium in that order. To examine the protein-bound polysaccharide from Ganoderma lucidum for immunopotentiating activity, its fruit bodies were extracted with hot water. Purification of the extract was carried out by acetone precipitation and dialysis. The fraction obtained during the purification procedure consisted of a polysaccharide moiety (51%) and a protein moiety (5%). When the compound was administered intraperitoneally to the mice at a dose of 50mg/kg, it enhanced the accumulation of the peritoneal exudate cells, macrophage and polymorphonuclear leucocytes, thereby indicating immunopotentiation.

• 한국유기농업학회지
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• 제13권3호
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• pp.291-300
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• 2005

우리나라 벼 유기농업 실천농가의 가장 큰 애로사항은 알려져 있는 병해 및 잡초 제어기술을 정립하기 위하여 강화 등 5개 지역의 벼 유기농재배지와 관행재배지에서 병해충의 발생 실태를 조사한 결과는 다음과 같다. 1. 병해 발생조사결과 도열병은 관행재배지보다 오리농법 재배지에서 발병이 많았고 품종별로는 추청벼 보다 일품벼에서 발병이 많았으며, 잎집무의마름병은 오리농법과 쌀겨농법재배지에서 발병이 많았고 품종별로는 추청벼 보다 일품벼에서 발병이 많았으며, 문고병도 관행재배지보다 오리농법과 쌀겨농법에서 발병이 많은 경향이었다. 2. 친환경 자재의 벼 종자소독 효과시험 결과 현미식초 1,000배액이 키다리병 방제에 효과가 있었으며 발아율도 양호하였고, 시중에 종자소독용으로 유통되고 있는 친환경자재의 도열병에 대한 예방 및 치료효과는 미미하였다. 3. 헤어리벳치 재배구의 헤어리벳치 건물 중이 이앙시기에 추천량의 1/40에 불과하여 헤어리벳치 재배에 의한 토양물리성의 변화는 없었다. 4. 헤어리베치 잎과 뿌리의 추출물질은 상추종자의 발아 억제효과를 보였다.

• 한국미생물·생명공학회지
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• 제34권4호
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• pp.318-322
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• 2006

알칼리와 고온에 대한 내성을 가진 invertase를 대량생산하기 위하여 호알칼리성이며 고온성 세균인 Bacillus sp. TA-11의 세포내 invertase유전자를 pUC 19벡터를 이용하여 E. coli HB101에 클로닝 시키고 발현시켜 invertase를 강력하게 생산하는 재조합 E. coli (pYC17)를 얻었다. 재조합 E. coli(pYC17)를 이용한 invertase 생산 최적조건을 검토한 결과 E. coil(pYC17)를 0.25% sucrose, 0.5% yeast extract, 0.1% $K_2HPO_4$와 0.1% $KH_2PO_4$을 함유한 SY배지(초기 pH 9.0)에 접종하여 37$^{\circ}C$에서 9시간 배양하였을 때 친주보다도 많은 47.7 U/ml-cell free extract의 invertase가 생산되었다.

• 한국수산과학회지
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• 제50권6호
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• pp.656-661
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• 2017

To develop a highly value-added product from extract from small and damaged sea mussels Mytilus edulis, we prepared two types of sea mussel sauce (MS): bottled (BMS) and retort pouched (RMS). We investigated the processing conditions, quality metrics and flavor compounds in each type of sauce. We found that the most appropriate base formulation for both BMS and RMS consisted of 40.0% SME (Brix $30^{\circ}$), 15.0% sugar, 6.0% salt, 4.0% monosodium glutamate, 4.0% soy sauce, 3.5% starch, 3.0% yeast extract, 3.5% wheat flour and 21.0% water. The crude protein, salinity, volatile basic nitrogen and amino-nitrogen content of the BMS and RMS were 8.7% and 8.8%, 9.3% and 9.2%, 24.9 and 31.4 mg/100 g, and 468.5 and 455.1 mg/100 g, respectively. For comparison, the ranges of these values in commercial oyster sauces (COS) are 4.7-7.5%, 10.7-12.0%, 8.2-12.5 mg/100 g, and 225.7-448.2 mg/100 g, respectively. The total free amino acid content of RMS and Premium COS was 7,215.7 and 6,160.7 mg/100 g, respectively, and the main free amino acids were glutamic acid, taurine, glycine, alanine, arginine, proline and lysine. These results demonstrate that BMS and RMS have favorable organoleptic qualities and good storage stability compared to COS, and are suitable for commercialization as high-flavor seasoning sauces.