• 한국직업건강간호학회지
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• 제20권3호
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• pp.250-260
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• 2011

Purpose: The purpose of this study was to describe the role experiences of occupational health nurse. The research question was "what are daily experiences in practice?" Methods: The data were collected through the focus-group interviews with nine occupational health nurses and analyzed by the grounded theory of Strauss and Corbin (1998). Results: The core category on occupational health nurse role experience was named as "struggling alone with the dual role". The causal conditions included 'unfamiliar area' and 'new work'. The contextual conditions included 'overload of work, 'tasks beyond OHN job' and 'disharmony with colleagues'. The intervening conditions were analyzed as supportive factors and discouraging factors. There were action/interaction strategies 'getting information about tasks', 'working as a professional', 'establishing a cooperative relationship', 'seeking help' by trying to regulate the phenomenon. Finally they succeed in 'establishing the identity' and 'feeling proud', but sometimes they could be 'suffering from low morale', or 'considering turnover'. Conclusion: Through these results, it can be explained that occupational health nurses have been exerting themselves to establish their role with various situation through the conflicted context. Based on the findings, we suggest developing the diverse continuing education programs to be tailored for OHN's needs and reinforcing the occupational health services under the legal and political support.

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 1988년도 학술대회지
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• pp.151-155
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• 1988

인삼사포닌, 프로토파낙사다이옥 사포닌 및 푸로토파낙사트리올 사포닌의 몰핀내성 및 의존성 형성억제 작용을 연구하였다. 인삼사포닌은 독성이 10배, 진통력은 1/2인 morphinone으로 대사시키는 morphine 6-hydrogenase의 작용을 억제시켜 morphinone의 생성을 증가시켜 morphine glutathione conjugation을 촉진시키므로 morphinone의 해독작용이 증가된다. 또한 인삼은 척수하행성 억제계의 활성을 억제하여 몰핀의 진통력을 길항하므로 신경계 기능상의 변화도 몰핀의 내성 및 의존성형성 억제작용에 관여하는 것으로 추정된다.

• The Korean Journal of Physiology and Pharmacology
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• 제12권5호
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• pp.253-258
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• 2008

Somatostatin (SOM) is a widely distributed peptide in the central nervous system and exerts a variety of hormonal and neural actions. Although SOM is assumed to play an important role in spinal nociceptive processing, its exact function remains unclear. In fact, earlier pharmacological studies have provided results that support either a facilitatory or inhibitory role for SOM in nociception. In the current study, the effects of SOM were investigated using anesthetized cats. Specifically, the responses of rostrally projecting spinal dorsal horn neurons (RPSDH neurons) to different kinds of noxious stimuli (i.e., heat, mechanical and cold stimuli) and to the $A{\delta}$ -and C-fiber activation of the sciatic nerve were studied. Iontophoretically applied SOM suppressed the responses of RPSDH neurons to noxious heat and mechanical stimuli as well as to C-fiber activation. Conversely, it enhanced these responses to noxious cold stimulus and $A{\delta}$-fiber activation. In addition, SOM suppressed glutamate-evoked activities of RPSDH neurons. The effects of SOM were blocked by the SOM receptor antagonist cyclo-SOM. These findings suggest that SOM has a dual effect on the activities of RPSDH neurons; that is, facilitation and inhibition, depending on the modality of pain signaled through them and its action site.

• 한국콘텐츠학회:학술대회논문집
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• 한국콘텐츠학회 2008년도 춘계 종합학술대회 논문집
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• pp.116-120
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• 2008

스마트 카메라는 종래의 획득한 영상을 압축하여 전송하는 네트워크 카메라 기능에 더하여, 획득한 영상을 해석하여 상황을 인지하고 이에 따른 실시간 조치가 가능한 지능 비젼 기능을 추가적으로 갖춘 카메라이다. 지능 비젼 알고리즘들은 연산량이 많다. 따라서 싱글 CPU로 영상을 압축하고 전송하는 일 뿐만 아니라 지능 비젼 처리까지 모두 실시간으로 처리하기에는 무리가 있다. Texas Instruments 사가 제공하는 다빈치 프로세서는 ARM 코어와 DSP 코어의 듀얼 코어이며 네트워킹 인터페이스 및 비디오 획득 인터페이스를 비롯하여 디지털 비디오 응용 임베디드 제품 개발에 필요한 다양한 I/O을 지원하는 인기 있는 ASSP(Application Specific Standard Product)이다. 본 논문에서는 다빈치 프로세서 기반 스마트 카메라의 S/W 를 설계하고 구현한 결과를 기술한다. 얼굴 검출 응용을 예로 구현하였고 동작이 잘 수행됨을 확인하였다. 향후 보다 광범위하고 실시간으로 동작되는 비젼 기능이 지원되는 스마트 카메라 개발을 위해 보다 효율적인 비젼 응용 S/W 구조와 알고리즘의 최적화에 대한 연구가 필요하다.

• Journal of Microbiology and Biotechnology
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• 제31권1호
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• pp.25-32
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• 2021

Inflammatory reactions activated by lipopolysaccharide (LPS) of gram-negative bacteria can lead to severe septic shock. With the recent emergence of multidrug-resistant gram-negative bacteria and a lack of efficient ways to treat resulting infections, there is a need to develop novel anti-endotoxin agents. Antimicrobial peptides have been noticed as potential therapeutic molecules for bacterial infection and as candidates for new antibiotic drugs. We previously designed the 9-meric antimicrobial peptide Pro9-3 and it showed high antimicrobial activity against gram-negative bacteria. Here, to further examine its potency as an anti-endotoxin agent, we examined the anti-endotoxin activities of Pro9-3 and elucidated its mechanism of action. We performed a dye-leakage experiment and BODIPY-TR cadaverine and limulus amebocyte lysate assays for Pro9-3 as well as its lysine-substituted analogue and their enantiomers. The results confirmed that Pro9-3 targets the bacterial membrane and the arginine residues play key roles in its antimicrobial activity. Pro9-3 showed excellent LPS-neutralizing activity and LPS-binding properties, which were superior to those of other peptides. Saturation transfer difference-nuclear magnetic resonance experiments to explore the interaction between LPS and Pro9-3 revealed that Trp3 and Tlr7 in Pro9-3 are critical for attracting Pro9-3 to the LPS in the gram-negative bacterial membrane. Moreover, the anti-septic effect of Pro9-3 in vivo was investigated using an LPS-induced endotoxemia mouse model, demonstrating its dual activities: antibacterial activity against gram-negative bacteria and immunosuppressive effect preventing LPS-induced endotoxemia. Collectively, these results confirmed the therapeutic potential of Pro9-3 against infection of gram-negative bacteria.

• The Korean Journal of Physiology and Pharmacology
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• 제26권1호
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• pp.15-24
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• 2022

The development of selective targeting of drug molecules towards the mitochondria is an important issue related to therapy efficacy. In this study, we report that gallic acid (GA)-mitochondria targeting sequence (MTS)-H₃R₉ exhibits a dual role as a mitochondria-targeting vehicle with antioxidant activity for disease therapy. In viability assays, GA-MTS-H₃R₉ showed a better rescue action compared to that of MTS-H₃R₉. GA-MTS-H₃R₉ dramatically exhibited cell penetration and intercellular uptake compared to MTS and fit escape from lysosome release to the cytosol. We demonstrated the useful targeting of GA-MTS-H₃R₉ towards mitochondria in AC16 cells. Also, we observed that the antioxidant properties of mitochondrial-accrued GA-MTS-H₃R₉ alleviated cell damage by reactive oxygen species production and disrupted mitochondrial membrane potential. GA-MTS-H₃R₉ showed a very increased cytoprotective effect against anticancer activity compared to that of MTS-H₃R₉. We showed that GA-MTS-H₃R₉ can act as a vehicle for mitochondria-targeting and as a reagent for therapeutic applications intended for cardiovascular disease treatment.

• BMB Reports
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• 제55권5호
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• pp.205-212
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• 2022

Interstitial cystitis/bladder pain syndrome (IC/BPS) is a debilitating chronic disorder characterized by suprapubic pain and urinary symptoms such as urgency, nocturia, and frequency. The prevalence of IC/BPS is increasing as diagnostic criteria become more comprehensive. Conventional pharmacotherapy against IC/BPS has shown suboptimal effects, and consequently, patients with end-stage IC/BPS are subjected to surgery. The novel treatment strategies should have two main functions, anti-inflammatory action and the regeneration of glycosaminoglycan and urothelium layers. Stem cell therapy has been shown to have dual functions. Mesenchymal stem cells (MSCs) are a promising therapeutic option for IC/BPS, but they come with several shortcomings, such as immune activation and tumorigenicity. MSC-derived extracellular vesicles (MSC-EVs) hold numerous therapeutic cargos and are thus a viable cell-free therapeutic option. In this review, we provide a brief overview of IC/BPS pathophysiology and limitations of the MSC-based therapies. Then we provide a detailed explanation and discussion of therapeutic applications of EVs in IC/BPS as well as the possible mechanisms. We believe our review will give an insight into the strengths and drawbacks of EV-mediated IC/BPS therapy and will provide a basis for further development.

• Molecules and Cells
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• 제45권8호
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• pp.537-549
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• 2022

Preproenkephalin (PPE) is a precursor molecule for multiple endogenous opioid peptides Leu-enkephalin (ENK) and Met-ENK, which are involved in a wide variety of modulatory functions in the nervous system. Despite the functional importance of ENK in the brain, the effect of brain-derived factor(s) on PPE expression is unknown. We report the dual effect of neural epidermal growth factor (EGF)-like-like 2 (NELL2) on PPE gene expression. In cultured NIH3T3 cells, transfection of NELL2 expression vectors induced an inhibition of PPE transcription intracellularly, in parallel with downregulation of protein kinase C signaling pathways and extracellular signal-regulated kinase. Interestingly, these phenomena were reversed when synthetic NELL2 was administered extracellularly. The in vivo disruption of NELL2 synthesis resulted in an increase in PPE mRNA level in the rat brain, suggesting that the inhibitory action of intracellular NELL2 predominates the activation effect of extracellular NELL2 on PPE gene expression in the brain. Biochemical and molecular studies with mutant NELL2 structures further demonstrated the critical role of EGF-like repeat domains in NELL2 for regulation of PPE transcription. These are the first results to reveal the spatio-specific role of NELL2 in the homeostatic regulation of PPE gene expression.

• 한국프랜차이즈경영연구
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• 제5권2호
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• pp.69-79
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• 2014

본 연구는 현 시대에 점점 증가하는 여가 스포츠인 골프에 대한 선택 기준이 일반인들의 신규 회원 가입결정에 어떠한 작용을 야기 시키는 지를 현실적으로 입증하고자 수행되었다.결과를 살펴보면 심층면담, 직접참여를 통해 "시간적 여유", "점점 저렴해지는 기자재", "다양해진 프로그램", "가까운 곳에 위치", "자기개발 및 대인관계"의 항목들이 여가 스포츠 중 일반인들이 선택하는데 있어 많은 영향을 미치는 것으로 나타났다. 이중에서도 가장 많은 답변을 지닌 것은 "점점 저렴해지는 기자재", "자기개발 및 대인관계"로 나타났다. 이 같은 결과는 각박한 현시대를 살아감에 있어 여가 스포츠를 선택하는 가운데 경제적 측면을 무시할 수 없는 것이 사회현상이며 자기계발 및 대인관계가 조직사회에서는 진급이나 매출향상, 신분상승 의 기회를 제공하는 핵심적 역할의 통로가 되고 있음을 제시하고 있다.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제23권2호
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• pp.107-114
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• 2001

Objectives : To compare jaw bone density of young adults (control group) and post-menopausal women(experimental group) in periapical and panoramic film. Materials and Methods : The bone mineral density values of lumbar and femur were measured by dual-energy X-ray absorptiometry(DEXA) and T scores of lumbar were obtained. T scores were classified into 3 group (T<-2.5, $-2.5{\leqq}T<-1$, $-1{\leqq}T$). Radiographic densities of alveolar bones were measured from interdental bones of premolar, molar areas in the maxilla and mandible and expressed into copper step wedge thickness by Scion $Image^{(R)}$ program. We considered these values of step wedge thickness as bone density of alveolar bone. Panorama mandibular index(PMI) was calculated by the method that the height of the inferior cortex of the mandible was divided by the height from the lower border of the mandible to the superior edge of the mental foramen. Bone density of alveolar bone and PMI were analysed statistically. Results : There were significant differences in bone mineral density of lumbar and femoral neck between control and experimental groups. There were also significant differences in bone density of premolar and molar area of jaw between control and experimental groups by MANOVA test. When considered lumbar T variables, there was only difference in interdental bone density of maxillary molar area between control and experimental group, but there was interaction. Interdental bone density of experimental group was appeared higher in $-1{\leqq}T$ group and lower in T<-2.5 group than control group. There was significant difference in PMI between control and experimental groups, but there was also inter action, thus, PMI of experimental group was appeared higher in $-1{\leqq}T$ group and lower in T<-2.5 group than control group. Conclusion : There were significant differences of alveolar density and cortical bone thickness between young men and post-menopausal women in periapical and panoramic film. These differences were dependent on lumbar T.