• Microbiology and Biotechnology Letters
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• v.37 no.1
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• pp.33-41
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• 2009

The crushed fruiting body of Alaskan Porodaedalea pini (Brot.) Murrill (syn. Phellinus pini) was extracted in boiling water for 4 h with the yield of 20.5% in dry mass. This hot-water extract showed significant antiviral activity by inhibiting the plaque formation in HeLa cells by coxackievirus B3 (CVB3) and also showed highest inhibitory effect against neuraminidase activity among water extracts of various mushrooms. From the water extract, the ethanol precipitate (EP) and supernatant fraction (ES) were obtained through 75% ethanol precipitation with the yield of 43.3% and 28.3% in dry mass, respectively. Whereas ES did not show any detectable level of antiviral activity, EP showed significant dose-dependent inhibition of plaque formation by CVB3 in HeLa cells with an $EC_{50}$ (50% effective concentration) of 0.45 mg/mL. The cytotoxicity on HeLa cells by EP was relatively low with the $CC_{50}$ (50% cytotoxic concentration) of 2.25 mg/mL. EP also effectively inhibited neuraminidase activity in a dose-dependent manner showing up to 75% inhibition at 1.7 mg/mL. These results suggest that the hot-water extract and its EP of P. pini fruiting body can be a candidate for the development of a potent broad-range antiviral agent against influenza virus(Flu) as well as CVB3. The major active component of EP was shown to be a heteropolysaccharide-protein complex containing glucose as the main sugar residue with mole percentage of 79.8% and other sugars like galactose (19.2%), xylose (17%), mannose (5.8%), and fucose (4.6%) and a small portion (12.7%, in mass) of protein.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.11 no.2
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• pp.68-81
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• 2006

As one of the on-going projects to investigate the biogeochemical characteristics of tidal flat, we develop seasonal mass balance calculations (or DIP, DIN and DON in Gomso Bay. We have obtained 13-hours time-series data of salinity, tidal current, nutrients, and chlorophyll-a of seawater for spring, dry summer, rainy summer and winter during $1999{\sim}2000$. DIP of $-1.10{\times}10^6g\;P\;day^{-1},\;-4.50{\times}10^5g\;P\;day^{-1}$ was out-fluxed from the bay to the bay proper for spring and dry summer, respectively. Whereas $1.06{\times}10^4g\;P\;day^{-1}$ of net influx of DIP was found during winter and $2.72{\times}10^6g\;P\;day^{-1}$ of net influx was also found during the rainy summer. Therefore we suggest the role of Gomso tidal flat as a source of DIP fur the seasons of spring and summer, but as an opposite role during the rainy summer and winter but much smaller in magnitude. Except winter, the advection process by tidal current is found the most dominant flux among the diverse fluxes of DIP in the bay. Whereas ground water is estimated as the strongest flux of TDN except winter. TDN of $1.38{\times}10^7g\;N\;day^{-1},\;2.45{\times}10^6g\;N\;day^{-1},\;and\;4.65{\times}10^7g\;N\;day^{-1}$ was in-fluxed to the bay from the bay proper far spring, rainy summer and summer, respectively. Only $-1.70{\times}10^7g\;N\;day^{-1}$ of net out-flux was found during the winter. Therefore we suggest the role of Gomso tidal flat as a sink of TDN far the year round except winter.

• Korean Journal of Soil Science and Fertilizer
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• v.29 no.2
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• pp.165-172
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• 1996

Symbiotic effectiveness and significance of Bradyrhizobium japonicum strains in five serogroups which were consisted of three corresponding serotype strains, respectively, indigenous to Korean soils were evaluated in terms of utility of strain serogroup for symbiotic improvement on soybean plant. The nodulation by rhizobia of each serogroup on four soybean cultivars(Danweonkong, Kwangkyo, Pangsakong, and Eunhakong) was good in order of USDA 123 > YCK 150 > YCK 117 > YCK 141 > USDA 110 serogroup members. Shoot dry weight of soybean was relatively high with USDA 110 serogroup members as well as with YCK 141 serogroup members, whereas the effectiveness of USDA 123 serogroup members was the lowest among the serogroups examined. In particular, Pangsakong among soybeans inoculated with five-serogroup members was positively outstanding on nodulation and shoot dry weight of the plant. Overall, symbiotic parameters of serogroup members associated with soybean plant such as nodule number, nodule mass, $N_2$ase activity, and shoot dry weight showed significantly different responses at level of 1% probability among both rhizobial serogroups and soybean cultivars, respectively. The rate in symbiotic similarity of the members of each serogroup from F-test ($$P{\leq_-}0.05$$) was 100% for nodule No., 90% for $N_2$ase activity. and 80% for soybean shoot dry weight. Taken together, the results indicated that the serological grouping of B. japonicum could be strongly useful for improving the symbiotic effectiveness hetween soybean and Rhizobium.

• Food Science of Animal Resources
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• v.31 no.6
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• pp.914-920
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• 2011

The aim of this study was to analyze the effects of salt and drying period on the physicochemical and sensory parameters of dry-cured ham ripened in a controlled condition. In this study, three treatments were performed: High salt group (HS), salted with 7 g $kg^{-1}salt$ (w/w); Middle salt group (MS), 5 g $kg^{-1}salt$ and; Low salt group (LS), 3 g $kg^{-1}salt$. Three conditions of drying period were applied including 180, 270 and 360 d at $19^{\circ}C$ and 65% relative humidity, and the physicochemical character and sensory properties of M. biceps femoris were investigated. pH and water activity were decreased with increasing drying period, and the pH of LS was higher than that of other treatments (p<0.05). When increasing the drying period, the hardness of HS was steadily raised for 360 d, whereas LS and MS hardened between 180 and 270 d (p<0.05), and there was no significant difference after 270 d. Cohesiveness, glumness and chewiness had a positive relationship with the drying period. Also, springiness, glumness and chewiness showed a positive relationship with salt level. Chroma and hue value were improved by increasing the salt level. As the drying period increased, the rate of hardness and flavor intensity was increased. In general, this research can be used as essential information for the mass production of dry cured ham.

• Korean Journal of Environmental Biology
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• v.38 no.3
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• pp.404-415
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• 2020

We investigated the seasonal variations in growth and physiological responses of the kelp species Ecklonia cava to different nitrogen sources to establish indoor culture conditions for mass production. Ecklonia cava was cultivated for 10 days in 16 combinations of seawater temperatures (15, 17, 21, and 25℃) and different nitrogen sources (control; NH-NH₄⁺ 100 μM; NO-NO₃^- 100 μM; and NHNO-NH₄⁺ 50 μM+NO₃^- 50 μM). The growth and growth rate of the blade were affected by temperature. The mean fresh weight and area-based daily growth rate were the highest (5.8±0.5 and 6.6±0.5% day^-1, respectively) at 15℃ and the lowest (2.2±0.2 and 3.0±0.3% day^-1, respectively) at 25℃. The daily growth rate was the highest in the NH and NO treatments and lowest in the control. The nitrate reductase activity of E. cava varied with water temperature (season). The highest activity was in the control (1.32±0.10 μmol NO₂^- g^-1 dry weight h^-1) and the lowest was in the NH treatment (0.25±0.02 μmol NO₂^- g^-1 dry weight h^-1). The photosynthetic pigment concentrations reached a maximum value in the NHNO treatment and a minimum value in the control. These results showed that water temperature played an important role in the cultivation of E. cava and that a single supply of NH₄⁺ or NO₃^- may induce the accelerated growth of E. cava. The growth and physiological responses of E. cava to different nitrogen sources during each season provide valuable information for determining the optimal nitrogen source in E. cava cultivation under indoor conditions.

• Journal of the Korean earth science society
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• v.33 no.3
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• pp.269-279
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• 2012

Satellite-retrieved data on Aerosol Optical Depth (AOD) and ${\AA}$ngstr$\ddot{o}$m exponent (AE) using a Moderate Resolution Imaging Spectrometer (MODIS) were used to analyze large-scale distributions of atmospheric aerosols in East Asia. AOD was relatively high in March ($0.44{\pm}0.25$) and low in September ($0.24{\pm}0.21$) in the East Asian region in 2009. Sandstorms originating from the deserts and dry areas in Northern China and Mongolia were transported on a massive scale during the springtime, thus contributing to the high AOD in East Asia. Although $PM_{10}$ with diameters ${\leq}10{\mu}m$ was the highest in February at Anmyon, Cheongwon and Ulleung, which is located leeward about half-way through the Korean Peninsula, AOD rose to a high in May. The growth of hygroscopic aerosols moving with increases in relative humidity prior to the Asian monsoon season contributed to a high AOD level in May. AE typically reaches its highest value ($1.30{\pm}0.37$) in August due to anthropogenic aerosols originating from industrial areas in Eastern China, while AOD stays low in summer due to the removal process caused by rainfall. The linear correlation coefficients of the MODIS AOD and ground-based mass concentrations of $PM_{10}$ at Anmyon, Cheongwon and Ulleung were 0.4-0.6. Four cases (six days) of mineral dustfall from sandstorms and six cases (twelve days) of anthropogenically polluted particles were observed in the central area of the Korean Peninsula in 2009. $PM_{10}$ mass concentrations increased at both Anmyon and Cheongwon in the cases of mineral dustfall and anthropogenically polluted particles. Cases of dustfall from sandstorms and anthropogenic polluted particles, with increasing $PM_{10}$ mass concentrations, exhibited higher AOD values in the Yellow Sea region.

• Journal of Environmental Science International
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• v.17 no.2
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• pp.211-224
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• 2008

Characteristics of the esterification reaction between free fatty acid in rice bran oil and methanol was investigated in the presence of catalysts, such as PTS(p-toluene sulfonic acid), Amberlyst 15 dry and SCX(silica gel based strong cation exchange resin). While reaction temperature was kept constant at $65^{\circ}C$, initial feed content of free fatty acid was varied from 100% to 1% by addition of pure free fatty acid which was previously made from rice bran oil. Also, the effect of mole ratio of methanol to fatty acid on the final conversion was examined. When esterification of pure free fatty acid was catalyzed by several acids, final conversions were increased in order of Amberlyst 15 dry, SCX and PTS. Using PTS catalyst, initially the reaction proceeded in homogeneous 2nd oder reaction mechanism. However, phase of reaction mixture changed from homogeneous to heterogeneous along the reaction time and then reaction rate was retarded by mass transfer resistance of methanol. Final conversion of free fatty acid in reaction mixture was depended on initial feed content of free fatty acid, and had maximum value at 30% of initial feed free fatty acid content for all kinds of catalysts used. And the final conversion was increased with mole ratio of methanol by the improvement of reaction rate. When initial feed free fatty acid content below 10% and the reaction was catalyzed by PTS, concentration of free fatty acid in reaction mixture was increased in the middle of reaction time by hydrolysis of triglyceride in reaction mixture. Also, if silica gel was added into the reaction mixture which had initial feed free fatty acid content below 50%, final conversion was increased by the adsorption of moisture produced. The SCX catalyst made the esterification reaction of free fatty acid to progress like in case of PTS catalyst. However, when initial feed free fatty acid content below 10%, concentration of free fatty acid in. reaction mixture was decreased monotonically and not increased in the middle of reaction time on the contrary to the case of PTS. Thus, SCX catalyst accomplished more high value of final conversion than PTS catalyst for the initial feed fatty acid content range from 50% to 5% In case of initial feed free fatty acid content of 1% and mole ratio of methanol was 2, concentration of free fatty acid in reaction mixture increased over the initial feed free fatty acid content for all kind of catalysts used. Although SCX catalyst was added into reaction mixture which had 1% of initial feed fatty acid content, final conversion was hardly raised by mole ratio of methanol.

• Journal of the Korean Society of Hazard Mitigation
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• v.9 no.4
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• pp.11-20
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• 2009

By using the artificial lightweight aggregate for the natural aggregate depletes and destruction of environment and the application of lightweight concrete in structure, the lightweight concrete is manufactured. The fundamental characteristics by the waterbinder ratio was evaluated. It is suggested the method to control of pre-absorbed water of the lightweight aggregate. Lightweight concrete with pre-absorbed aggregate has similar characteristics compared to normal weight concrete regardless of water-binder ratio. According to the water-binder ratio, the drying condition, and the rebar, the unit mass of the lightweight concrete showed the reduction of 14.6${\sim}$21.0% as the range of 1,668${\sim}$1,998 $kg/m^3$ in comparison to the normal weight concrete. The lightweight aggregate pre-absorbed water showed the deferent evaporation quantity according to the water-binder ratio. As the water-binder ratio is lower, the oven dry vapour water is larger, therefore the internal curing water is increasing. In the same water-binder, comparing the normal concrete the lightweight concrete shows lower compressive strength which is due to the different strength of an aggregate. In the air dry curing, the normal weight concrete has a lower strength improvement effect in w/c 0.3 than the ratio 0.4 and 0.5. However, the strength improvement effect has increasing as the water-binder ratio was low in the light concrete.

• Journal of Korea Water Resources Association
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• v.36 no.6
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• pp.1013-1023
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• 2003

This study was performed to improve water quality of stream by applying hydraulic structures (weir and river bed material) made of porous concrete. The physical and chemical characteristics of porous concrete were measured to estimate application possibility of it in hydraulic structures and it was considered as a proper material for the hydraulic structures. In the results of comparison for the component of matters attached on the hydraulic structures made of porous and ordinary concrete, DW (dry weight) amount attached on porous concrete was 1.6 times higher than that on ordinary concrete under the condition of the same flow rate but influence by flow rate (difference of 10 times) was not shown. Therefore, we could understand that the material of media was more important in DW amount than flow rate. The rate of AFDM (ash free dry mass) to DW also was more at porous concrete than at ordinary concrete. Especially, the high rates of nitrogen and phosphorous in matters attached on porous concrete verify that they were removed by assimilation, adsorption and metabolism of periphyton. The removal percentage of SS, BOD, COD, T-N and T-P by hydraulic structures applying porous concrete compared with ordinary concrete was increased by 34.6%, 36.9%, 33.9%, 18.3% and 21.6%, respectively. Therefore, applying porous concrete to hydraulic structure is expected to contribute to improvement of stream water quality.

• Proceedings of the Korean Society of Near Infrared Spectroscopy Conference
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• 2001.06a
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• pp.1510-1510
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• 2001

NIRS uses reflectance signals resulting from bending and stretching vibrations in chemical bonds between carbon, nitrogen, hydrogen, sulfur and oxygen. These reflectance signals are used to measure the concentration of major chemical composition and other descriptors of homogenized and freeze-dried whole broiler carcasses. Six strains of chicken were analyzed and the NIRS model predictions compared to reference data. The results of this comparison indicate that NIRS is a rapid tool for predicting dry matter (DM), fat, crude protein (CP) and ash content in the broiler carcass. Males and females of six commercial strain crosses of broiler chicken (Gallus domesticus) were used in this study (6$\times$2 factorial design). Each strain was grown to 16 weeks of age, and duplicate serial samples were taken for body composition analysis. Each whole carcass was pressure-cooked, homogenized, and a representative sample was freeze-dried. Body composition determined as follows: DM by oven dried method at 105$^{\circ}C$ for 3 hours, fat by Mojonnier diethyl ether extraction, CP by measuring nitrogen content using an auto-analyzer with Kjeldhal digest and ash by combustion in a muffle furnace for 24 hour at 55$0^{\circ}C$. These homogenized and freeze-dried carcass samples were then scanned with a Foss NIR Systems 6500 visible-NIR spectrophotometer (400-2500nm) (Foss NIR Systems, Silver Spring, MD., US) using Infra-Soft-International, ISI, WinISl software (ISI, Port Matilda, US). The NIRS spectra were analyzed using principal component (PC) analysis. This data was corrected for scatter using standard normal “Variate” and “Detrend” technique. The accuracy of the NIRS calibration equations developed using Partial Least Squares (PLS) for predicting major chemical composition and carcass descriptors- such as body mass (BM), bird dry matter and moisture content was tested using cross validation. Discrimination analysis was also used for sex and strain identification. According to Dr John Shenk, the creator of the ISI software, the calibration equations with the correlation coefficient, $R^2$, between reference data and NIRS predicted results of above 0.90 is excellent and between 0.70 to 0.89 is a good quantifying guideline. The excellent calibration equations for DM ($R^2$= 0.99), fat (0.98) and CP (0.92) and a good quantifying guideline equation for ash (0.80) were developed in this study. The results of cross validation statistics for carcass descriptors, body composition using reference methods, inter-correlation between carcass descriptors and NIRS calibration, and the results of discrimination analysis for sex and strain identification will also be presented in the poster. The NIRS predicted daily gain and calculated daily gain from this experiment, and true daily gain (using data from another experiment with closely related broiler chicken from each of the six strains) will also be discussed in the paper.