• 제목/요약/키워드: drosophila melanogaster

검색결과 217건 처리시간 0.024초

Comparative analyses of susceptibility to chemicals associated with fermentation between Drosophila melanogaster and Drosophila suzukii

  • KIM, YiSeul;LEE, Sungho;KIM, Yeong Ho;KIM, Young Ho
    • Entomological Research
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    • 제48권6호
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    • pp.514-521
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    • 2018
  • Drosophila melanogaster Meigen and Drosophila suzukii Matsumura are taxonomically close Drosophila species belonging to the family Drosophilidae and melanogaster group. D. melanogaster is thought to be evolutionarily adapted to overripe, decaying, and fermented fruits, in which large amounts of chemicals such as ethanol, acetic acid, and 2-phenylethanol are produced, whereas, D. suzukii is attracted to fresh ripening fruit. Considering the distinct habitats of the two flies, D. suzukii is hypothesized to exhibit higher susceptibility to these chemicals than D. melanogaster. Therefore, in this study, we investigated the survival rate of the flies at various concentrations of three chemicals (2-phenlyethanol, acetic acid, and ethanol) and calculated the lethal concentration (LC) values to compare the tolerance and susceptibility of D. melanogaster and D. suzukii to the chemicals. Our results revealed that D. melanogaster exhibited higher tolerance than D. suzukii to all chemicals, supporting the hypothesis of different evolutionary adaptations to distinct habitats of the two flies.

초파리(Drosophila melanogaster)의 CdR Aminohydrolase에 관한 연구 (A Study on the CdR Aminohydrolase in Drosophila melanogaster)

  • 강만식
    • 한국동물학회지
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    • 제20권3호
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    • pp.129-134
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    • 1977
  • CdR aminohydrolase의 활성을 老化와의 관련하에서 보기 위해서 초라피(Drosophila melanogaster)의 이효소의 활성을 발생단계별로 측정하여 다음의 결과를 얻었다. 1. 초파리에서 CdR이 분해되는 과정은 쥐나 생쥐와 마찬가지로 $CdR \\to UdR \\to U$임을 알수 있었다. 2. CdR-aminohydrolase외 활성은 유충과 번데기에서 검출이 불가능하였고 성체에서만 검출되었다. 3. 성체의 효소활성은 젊은 개체에 비해서 늙은 개체에서 증가를 보였다. 4. 실험의 결과는 CdR-aminohydrolase의 활성이 초파리의 老化또는 발생단계와 관련성이 있음을 시사하였다.

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Drosophila melanogaster comple 3종 및 그들간 잡종의 성즐과 생식궁의 표현형적 유연관계 (Phenogenetical Relationships of Sex Comb and Genital Arch in Drosophila melanogaster Complex and Their Hybrids)

  • 최영현;유미애;이원호
    • 한국응용곤충학회지
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    • 제34권4호
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    • pp.308-313
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    • 1995
  • Drosophila melanogaster와 D. Simulan는 전세계적으로 분포하며, D. Sechellia 는 Africa의 Seychelles 제도에만 서식하는 지역종으로, 모두 D. melanogaster complex에 속하는 동포종이다. 이들 D. melanogaster complex 3종을 대상으로 종간 교배를 통한 잡종을 형성하여 성증(sex-bomb)과 생식궁(genital arch)의 표현형적 유연관계를 부모계통과 상호 비교하였다. 상기 3종들 사이의 교배를 통해 4가지 유형의 잡종 수컷을 얻었으며 이들은 모두 불임이었다. 성즐의 평균 치열 수는 D. melanogaster(OR)가 10.73개, D. sechellia(Ja)는 10.69개였으며, D. simulans($O_9$)에서는 8.35개였다. 종간 잡종의 치열수 분석으로 뚜렷한 성즐의 유전양식을 제시할 수가 없었다. 외부 생식기를 구성하는 각 부속기관 중 분류상 중요 기준이 되는 생식궁은 종 특이적 모양을 띠고 있었으며, 종간 접종 생식궁의 일반적인 형태는 부모종간의 중간형인 mosaic 구조였다.

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Drosophila melanogaster와 D. simulans 및 그들간 잡종의 형태학적 유연관계 (Morphological Relationship between Drosophila melanogaster, D. simulans and their Hybrids)

  • 최영현;유미애;이원호
    • 한국응용곤충학회지
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    • 제32권2호
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    • pp.193-199
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    • 1993
  • Drosophila melanogaster(OR)와 D. simulans(09) 및 그들 종간 잡종 사이의 형태적 유연관계를 비교하기 위하여 내.외부생식기 및 성즐을 조사하였다. 이를 위하여 D. melanogaster 암컷과 D. simulans 수컷의 교배에서 암컷을, 그 정역 교배로서 수컷을 얻었으며 두 종간 사이의 잡종 $F_{1}$들은 모두 불임이었다. 내부생식기의 비교에서, 두종간 (melanogaster female $\times$ simulans male) 잡종 {$F_{1}$ 암컷의 경우는 양쪽 난소가 모두 퇴화된 형태였으며, 그 정역교배에 의한 $F_{1}$ 수컷의 정소는 미발달된 상태로 남아 있었다. 이런 형태적 특징은 D. melanogaster P-M system에서의 hybrid dysgensesis에 의한 양상과 매우 유사하였다. 수컷의 앞다리 제1부절에 존재하는 성즐의 평균 치열수는 D. melanogaster와 D. simulans에서 각각 10.73개와 8.35개 였으며, 잡종 $F_{1}$ 수컷의 경우는 9.97개 정도였다. 두 종간 잡종 $F_{1}$ 수컷의 외부 생식기의 전체적인 형태는 D. melanogaster와 비슷하였으나, 부분적으로 D. simulans에 유사하거나 중간적인 형질을 가지는 mosaic 구조였다.

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Analysis of Two Promoters that Control the Expression of the GTP cyclohydrolase I Gene in Drosophila melanogaster

  • Byun, Jaegoo;Yoon, Jaeseung;Baek, Kwanghee
    • Molecules and Cells
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    • 제27권5호
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    • pp.583-589
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    • 2009
  • GTP cyclohydrolase I (GTPCH) is a key enzyme in the de novo synthesis of tetrahydrobiopterin. Previously, the Drosophila melanogaster GTPCH gene has been shown to be expressed from two different promoters (P1 and P2). In our study, the 5'-flanking DNA regions required for P1 and P2 promoter activities were characterized using transient expression assay. The DNA regions between -98 and +31, and between -73 and +35 are required for efficient P1 and P2 promoter activities, respectively. The regions between -98 and -56 and between -73 and -41 may contain critical elements required for the expression of GTPCH in Drosophila. By aligning the nucleotide sequences in the P1 and P2 promoter regions of the Drosophila melanogaster and Drosophila virilrs GTPCH genes, several conserved elements including palindromic sequences in the regions critical for P1 and P2 promoter activities were identified. Western blot analysis of transgenic flies transformed using P1 or P2 promoter-lacZ fusion plasmids further revealed that P1 promoter expression is restricted to the late pupae and adult developmental stages but that the P2 promoter driven expression of GTPCH is constitutive throughout fly development. In addition, X-gal staining of the embryos and imaginal discs of transgenic flies suggests that the P2 promoter is active from stage 13 of embryo and is generally active in most regions of the imaginal discs at the larval stages.

노랑초파리(Drosophila melanogaster)와 그 동포종들에 있어서 Gramoxone의 독성에 관한 비교 연구 (Comparative Toxic Effects of Gramoxone in the D. melanogaster and its Sibling Species)

  • 최영현;이원호;유미애
    • 한국환경과학회지
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    • 제1권2호
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    • pp.1-13
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    • 1992
  • Physiological toxic and mutagenic effects of gramoxone in Drosophila melanogaster were invetigated. Gramoxone was highly toxic on the development, resulting in of lowering the viability and in prolongation of the developmental times. Adults treated with gramoxone during the developmental stages caused a lowering of the productivity and a little chinge in protein quantity. But the effect on the sex-linked lethal mutagenesis was found to be negative. The order of mortality causing ado리t stoa형e feeding to gramoxone in the D. melanogaster complex was like this ; D. mauritiana, D. sechellia, D. simulans and D. melanogasteu Two species of the D. yakuba complex were alike. Those results were more or less correlation with speciation of the D. melanogaster subgroup.

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언양 자연 집단내 Drosophila melanogaster의 유해 유전자 빈도 분석 (Analysis of Frequencies of Deleterious Chromosomes in On-yang Natural Population of Drosophila melanogaster)

  • 김영필;최영현
    • 한국환경과학회지
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    • 제5권1호
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    • pp.1-6
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    • 1996
  • The genetic variabilities of second chromosomes of Drosophila melanogaster concealed in On-yang natural Population have been analyzed by the Cy/Pm method and an allelism test during two years(1993-1994). The mean frequencies of deleterious(lethal and semilethal) genes in On-yang natural population were estimated to be 23.97% in 1993 and 27.15% in 1994, respectively. The allelism rates between lethal genes in the population were 0.654%(1993) and 1.429%(1994). The mean values of elimination by frequencies of deleterious genes and allelism rates were 0.0004(1993) and 0.0010(1994), respectively. The frequencies of phenotypic sterility of males in 1994 were estimated to be 1.95%, and thoses of genotypic sterility of females and males were estimated to be 1.54% and 2.31%, respectively.

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Drosophila melanogaster as a Model for Studying Aspergillus fumigatus

  • AL-Maliki, Hadeel Saeed;Martinez, Suceti;Piszczatowski, Patrick;Bennett, Joan W.
    • Mycobiology
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    • 제45권4호
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    • pp.233-239
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    • 2017
  • Drosophila melanogaster is a useful model organism that offers essential insights into developmental and cellular processes shared with humans, which has been adapted for large scale analysis of medically important microbes and to test the toxicity of heavy metals, industrial solvents and other poisonous substances. We here give a brief review of the use of the Drosophila model in medical mycology, discuss the volatile organic compounds (VOCs) produced by the opportunistic human pathogen, Aspergillus fumigatus, and give a brief summary of what is known about the toxicity of some common fungal VOCs. Further, we discuss the use of VOC detection as an indirect indicator of fungal growth, including for early diagnosis of aspergillosis. Finally, we hypothesize that D. melanogaster has promise for investigating the role of VOCs synthesized by A. fumigatus as possible virulence factors.

Maternal Exposure to Bisphenol A Impacts on Fecundity in F1 and F2 Generations in Drosophila melanogaster

  • Kim, Sohee;Kang, Kyong-hwa;Koh, Hyongjong
    • 한국발생생물학회지:발생과생식
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    • 제25권3호
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    • pp.193-197
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    • 2021
  • In previous reports, bisphenol A (BPA) exposure affects reproductive function in Drosophila melanogaster females. To test the maternal effect of BPA exposure on fly reproductive function, F0 mothers were exposed to 0, 0.1, 1, and 10 mg/L of BPA and the fecundity in F1 and F2 generations were checked. In this experiment, 1 and 10 mg/L BPA significantly decreased the fecundity of F1 females. Moreover, 0.1 and 1 mg/L BPA substantially reduced egg production in the F2 generation. These results suggested that maternal exposure to BPA at enviromentally relavant concnetrations reduces reproductive function in Drosophila melanogaster females and that this effect is transgenerational.

세계 14지역 계통에 대한 초파리 미토콘드리아 DNA의 다형현상 (Mitochondrial DNA polymorphism in Fourteen Geographical Strains of Drosophila melanogoater)

  • 김봉기
    • 한국동물학회지
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    • 제31권3호
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    • pp.218-224
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    • 1988
  • Drosophila melanogaster의 세계 14지역 계통으로부터 mitochondrial DNA(mtDNA)를 추출하여, 제한 요소에 의하여 mtDNA종내 변이를 조사하였다. 그 결과, site variation(Hpall와 Haelll 및 Seal효소)과 length variation(최대550bp)이 나타났다. 또한 6종류(Ml, M2, M3, M4, M6 및 M7)의 mtDNA genotype이 검출되었으며, 종내 평균 염기 치환율은 1.88%로써 낮은 지역 분화(low divergence)를 나타내었다. 그러나 일본의 Ogasawara계통의 M5 type은 본 연구에서는 검출되지않았다. 이처럼 지역 계통간의 낮은 지역 분화는 세계14지역 계통의 D. melanogaster가 최근에 소수의 개체로부터 확산되었기 때문에 집단전체에 아직 충분한 mtDNA변이가 축적되지 않았거나 혹은 지리적 격리가 충분함에도 불구하고 지역 계통간에 빈번한 migration이 일어났기 때문에 mtDNA의 지역 분화가 방해되지않은 것으로 추측된다.

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