• 한국수정란이식학회지
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• 제31권1호
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• pp.1-7
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• 2016

Xenotransplantation involves multiple steps of immune rejection. The present study was designed to produce nuclear transfer embryos, prior to the production of transgenic pigs, using fibroblasts carrying transgenes human complement regulatory protein hCD59 and interleukin-18 binding protein (hIL-18BP) to reduce hyperacute rejection (HAR) and cellular rejection in pig-to-human xenotransplantation. In addition to the hCD59-mediated reduction of HAR, hIL-18BP may prevent cellular rejection by inhibiting the activation of natural killer cells, activated T-cell proliferation, and induction of $IFN-{\gamma}$. Transgene construct including hCD59 and ILI-18BP was introduced into miniature pig fetal fibroblasts. After antibiotic selection of double transgenic fibroblasts, integration of the transgene was screened by PCR, and the transgene expression was confirmed by RT-PCR. Treatment of human serum did not affect the survival of double-transgenic fibroblasts, whereas the treatment significantly reduced the survival of non-transgenic fibroblasts (p<0.01), suggesting alleviation of HAR. Among 337 reconstituted oocytes produced by nuclear transfer using the double transgenic fibroblasts, 28 (15.3%) developed to the blastocyst stage. Analysis of individual embryos indicated that 53.6% (15/28) of embryos contained the transgene. The result of the present study demonstrates the resistance of hCD59 and IL-18BP double-transgenic fibroblasts against HAR, and the usefulness of the transgenic approach may be predicted by RT-PCR and cytolytic assessment prior to actual production of transgenic pigs. Further study on the transfer of these embryos to surrogates may produce transgenic clone miniature pigs expressing hCD59 and hIL-18BP for xenotransplantation.

• Journal of Chest Surgery
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• 제47권2호
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• pp.145-148
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• 2014

Here, we present a rare case of cardiac parasitic infection found in an adult female patient who had the symptoms of dyspnea upon exertion. She was diagnosed with a double-chambered right ventricle due to infundibular hypertrophy confirmed by transthoracic echocardiography and cardiac computed tomography. We performed surgery of infundibulectomy around the pulmonary valve. In the end, histopathological findings of the resected infundibular muscle demonstrated trichinellosis, a type of roundworm infection.

• Journal of Chest Surgery
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• 제16권1호
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• pp.34-39
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• 1983

The syndrome of anomalous muscle bundle dividing the right ventricle into two pressure chambers has been described by many authors. The malformation should not be confused with tetralogy of Fallot itself because the obstructive hypertrophic bands are usually proximal to the right ventricular infundibulum. One case [11 years old male] of double chambered right ventricle due to aberrant muscle bundle with intact ventricular septum is presented. The pressure gradient was 68 mmHg between inflow and outflow tracts of right ventricle on cardiac catheterization. On opening the right ventricle, there noted stenosis of outflow tract by infundibular membrane, hypertrophied anomalous muscle bundle, thickened moderator band & hypertrophied anterior papillary muscle. Open heart surgery was carried out with the aid of extracorporeal support, Anomalous muscle bundle [1.0 cm x 4.0 cm] and infundibular membrane were resected safely. The hypertrophied moderator band was cut at mid-portion and anterior papillary muscle was split vertically. The postoperative course was uneventful and discharged in good condition 2 weeks later.

• 한국정보디스플레이학회:학술대회논문집
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• 한국정보디스플레이학회 2007년도 7th International Meeting on Information Display 제7권2호
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• pp.1327-1329
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• 2007

We developed a 4 inch (qVGA, 320x240) a-Si TFT LCD which has the function of color scanner. We have designed the novel pixel structure and got good scanning quality with minimum aperture loss. In this new pixel, the sensor capacitance was increased in double without decreasing the aperture loss.

• 한국전문물리치료학회지
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• 제22권3호
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• pp.91-97
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• 2015

This study was conducted to investigate the effects of the removable ankle-foot orthosis (RAFO) which was developed to improve the gait of stroke patients. The subjects of this study were five stroke patients who agreed to participate in this study by signing a written consent form. To verify gait improvement after wearing the orthosis, a Timed Up and Go test and Functional Gait Assessment were performed, and spatiotemporal gait variables such as gait speed, cadence, stride length, double limb support, and the efficient gait test of body sway angle were performed. For every variable, the differences prior to and after wearing the RAFO were compared using the Wilcoxon signed-rank test. Every gait variable improved significantly after wearing the RAFO compared to prior to wearing it. The pilot study will enhance future efforts to evaluate orthotic function objectively during gait in stroke patients.

• 한국해양공학회지
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• 제3권2호
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• pp.601-601
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• 1989

The behavior of fatigue was studied by using low carbon steel bar with microhole(artificial small defect) under the condition of rotary bending stress which is made artificially at smooth surface and round notch root. The results obtained can be summerized; The behavior of non-propagating cracks which are produced at both tips of small defect occurred to dissymmetry, and it is found to be double size of small defect. For the range of l>lc, threshold stress intensity is constant. However, for the range of l

• 한국해양공학회지
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• 제3권2호
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• pp.101-107
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• 1989

The behavior of fatigue was studied by using low carbon steel bar with microhole(artificial small defect) under the condition of rotary bending stress which is made artificially at smooth surface and round notch root. The results obtained can be summerized; The behavior of non-propagating cracks which are produced at both tips of small defect occurred to dissymmetry, and it is found to be double size of small defect. For the range of l>lc, threshold stress intensity is constant. However, for the range of l

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2009년도 춘계학술대회 논문집
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• pp.98-102
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• 2009

The magneto-rheological fluid expresses different cohesiveness according to the strength of the external electric current. To manufacture the magneto-rheological fluid damper that uses such characteristics of the fluid, a flow analysis of the inner damper was conducted to forecast the damper's capacity. In addition, using the finite element method software, analysis on the characteristics of electromagnetic field around the coil operation unit inside the damper. Based on the result of the analysis, a single core damper and a double core damper were built and tested for their dynamic function. Based on the result of the experiment, the propriety of the flow analysis was demonstrated, and the proposed model was verified.

• 한국소음진동공학회논문집
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• 제22권7호
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• pp.650-658
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• 2012

This paper proposes a high performance position controller for a driving system using a time optimal controller which has been widely used to control driving systems to achieve desired reference position or velocity in a minimum response time. The main purpose of this research lies in an improvement of transient response performance rather than that of steady-state response in comparison with other control strategies. In order to refine the scheme of time optimal control, Lyapunov stability proofs are incorporated in a controller of standard second order system model. This scheme is applied to the control of a driving system. In view of the simulation and experiment results, the standard second order system model exhibits better minimum-time control performance and robustness than double integral system model does.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2011년도 제40회 동계학술대회 초록집
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• pp.416-416
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• 2011

태양전지의 고효율화를 위해, 생성된 전자와 전공을 전극에서 효과적으로 수집하는 방법에 대한 연구가 많이 진행되고 있다. 태양전지 전면전극은 빛의 조사에 의해 생성된 전자를 수집하는 매체로써 finger width가 넓어질수록, 전자를 수집하기 쉬워진다. 하지만 finger width가 넓어짐에 따라, shadowing loss 증가에 의해 단락전류 밀도가 감소하여 태양전지 효율이 저하 될 수 있다. 본 연구에서는 스크린 프린팅에 의해 형성된 전면전극의 finger width를 기존의 $80{\mu}m$에서 $50{\mu}m$로 변경하고, double printing에 의해 finger height를 높이는 방법으로 태양전지의 효율을 향상 시키고자 하였다. 그 결과, 전극패턴 50에서는 전극패턴 80보다 0.47 단락전류밀도가 증가하였고, 효율(efficiency)은 0.16%가 증가하는 것을 확인할 수 있었다.